124 Effect of transvaginal oocyte aspiration on equine blood and peritoneal fluid parameters

2021 ◽  
Vol 33 (2) ◽  
pp. 170
Author(s):  
D. Orellana-Guerrero ◽  
E. Santos-Villanueva ◽  
S. Koshak ◽  
A. De La Fuente ◽  
G. Dujovne
Keyword(s):  
Peritoneal Fluid ◽  
Complete Blood Count ◽  
Invasive Procedure ◽  
Blood Chemistry ◽  
Ultrasound Probe ◽  
Transvaginal Aspiration ◽  
Clinical Responses ◽  
Oocyte Aspiration ◽  
The Relationship ◽  
Fluid Parameters

Transvaginal aspiration of oocytes (TVA) in the equine industry has gained more relevance and become a valuable technique to produce offspring from subfertile mares. TVA is a semi-invasive procedure and requires handling the ovaries transrectally to position them closely to an ultrasound probe located in the mare’s vagina. Once the ovary lies in close apposition to the ultrasound probe, a 12G needle is inserted through the needle guide, puncturing, aspirating, and scraping each follicle to recover the oocyte. Potential complications described include rectal tears, puncturing of blood vessels, ovarian abscesses, and peritonitis. Occasionally, problems occur after uneventful procedures, such as colic, peritonitis, pain, and anorexia. However, the source of these complications is not fully known. We hypothesize that blood and peritoneal fluid parameters would differ pre- and post-TVA in mares. A few reports provide some parameters after TVA (e.g. peritoneal protein, neutrophils, nucleated cells) without reference to pre-TVA values. These studies have not identified an effect in peritoneal fluid variables due to multiple abdominocenteses. Therefore, our aim was to analyse blood and peritoneal fluid in mares pre- and post-TVA, and to identify changes in parameters of the procedure (duration, number of pokes, number of follicles) and the mares’ clinical responses. Ten healthy mares were selected to undergo the procedure. Thirty minutes before starting TVA, a blood sample was drawn for complete blood count (CBC) and blood chemistry, and abdominocentesis was performed to obtain abdominal fluid and assess the cytology. This same protocol was repeated 24 hours after TVA. Physical exams were performed pre- and post-TVA. Paired t-tests were used to identify differences between groups (pre- and post-TVA). Spearman correlations (ρ) were used to assess the relationship between variables. There was a significant increase in peritoneal lactate (5.65-fold), peritoneal total protein (2.4-fold), and total nucleated cells (46-fold) between pre- and post-samples. These parameters were not associated with operator, number of times the needle was introduced into the ovaries, or number of aspirated follicles. The remaining parameters evaluated in CBC and blood chemistry did not differ. A positive correlation between total peritoneal protein and blood albumin was found post-TVA (ρ=0.72, P=0.01) but not pre-TVA (ρ=−0.1, P=0.65), suggesting an increase in protein level due to bleeding. Clinically, 9 mares were healthy throughout the study except one that presented signs of pain (facial grimace, anorexia, hyperthermia) the day following TVA. In conclusion, we showed changes in the peritoneal fluid during uneventful TVA procedures. The information provided by this research gives further insight into changes potentially caused by a TVA in abdominal fluid parameters. Further studies are necessary to determine expected standards and the duration of the changes after TVA.

scholarly journals Multidisciplinary Treatment after Orthodontics: Crown Lengthening and Dental Whitening in the Final Aesthetic Resolution of Smile

2020 ◽  
Vol 24 (4) ◽  
pp. 402-409
Author(s):  
Thais Akemi Sako ◽  
Geórgia Rondó Peres ◽  
Diego Oliveira Bavaresco ◽  
Danielle Gregorio ◽  
Larissa Sgarbosa de Araujo Matuda ◽  
...  
Keyword(s):  
Orthodontic Treatment ◽  
Correct Diagnosis ◽  
Multidisciplinary Treatment ◽  
Invasive Procedure ◽  
Tooth Bleaching ◽  
Tooth Whitening ◽  
The Aesthetic ◽  
Smile Aesthetics ◽  
Dental Whitening ◽  
The Relationship

Após a conclusão do tratamento ortodôntico, os pacientes começam a se preocupar com outros fatores estéticos para garantir um sorriso harmonioso, sendo mais exigentes com a aparência. Além de dentes alinhados e harmoniosos, os pacientes estão em busca de um sorriso mais branco, se tornando cada vez mais exigentes com a aparência, cabendo ao dentista atender a essas necessidades. Um outro fator que pode tornar o sorriso antiestético é a presença de dentes curtos, que resulta em exposição excessiva da gengiva e um sorriso com aspecto mais infantil, e que muitas vezes é percebido apenas a finalização do tratamento ortodôntico. O objetivo do presente estudo foi revisar o tratamento estético do sorriso após ortodontia, associando cirurgia periodontal para aumento de coroa clínica e clareamento dental. O sorriso gengival pode ser corrigido integrando várias especialidades odontológicas devido às suas inúmeras causas, podendo ser indicada a realização de procedimentos cirúrgicos, ortodônticos ou restauradores. Quanto ao amarelamento dos dentes, uma das técnicas utilizadas é o clareamento dental, que consiste num procedimento pouco invasivo, indicado na maioria dos casos de manchas extrínsecas. O clareamento pode ser caseiro ou de consultório, mas em ambos os casos, o cirurgião-dentista precisa dominar a técnica. Nesse contexto, cabe ao ortodontista identificar e diagnosticar essas necessidades do paciente e encaminhá-lo para outros especialistas. Em conclusão, para alcançar os resultados esperados pelo paciente são imprescindíveis uma boa anamnese, um correto diagnóstico e o planejamento multidisciplinar do caso. Portanto a inter-relação das especialidades é imprescindível a fim de obter resultados satisfatórios e agradáveis ao paciente.   Palavras-chave: Periodontia. Gengivectomia. Estética Dentária. Sorriso. Clareamento Dental.   Abstract After the end of orthodontic treatment, patients become more rigorous with their appearance and worry about other aesthetic factors to ensure a harmonious smile. In addition to aligned and harmonious teeth, patients are looking for a whiter smile, becoming more and more rigorous with their appearance, leaving the dentist to meet these needs. Another factor that can impair smile aesthetics is the presence of short teeth, which results in an overexposure of the gum and a smile with a more childlike aspect, and that it is often perceived only at the end of orthodontic treatment. The aim of the present study was to review the aesthetic treatment of the smile after orthodontics, associating periodontal surgery to increase the clinical crown and tooth whitening. Aesthetics is the relationship between several factors. The gummy smile can be corrected by integrating several dental specialties, due to its numerous causes, and surgical, orthodontic or restorative procedures may be indicated. For tooth whitening, one of the techniques most indicated is dental bleaching, which consists of a minimally invasive procedure, indicated in most cases of extrinsic stains. The whitening can be made at home or in office, but in both cases, the dentist needs to master the technique. In this context, it is up to the orthodontist to identify and diagnose these needs of the patient and refer him to other specialists. In conclusion, a good anamnesis, a correct diagnosis and multidisciplinary case planning are essential to achieve the results expected by the patient. Therefore, the interrelationship between specialties is important in order to obtain satisfactory and pleasant results for the patient.   Keywords: Periodontics, Gingivectomy, Esthetics, Smiling. Tooth Bleaching.

scholarly journals Parathyroid Hormone Levels in the Prediction of Ischemic Stroke Risk

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Güner Çelik ◽  
Ali Doğan ◽  
Şefik Dener ◽  
Şerefnur Öztürk ◽  
Sevsen Kulaksızoğlu ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Stroke Risk ◽  
Blood Chemistry ◽  
Dihydroxyvitamin D ◽  
Stroke Risk Factors ◽  
Ischemic Stroke Risk ◽  
Age Range ◽  
The Relationship ◽  
Estimation Models

Objective. It was examined whether PTH and 25-dihydroxyvitamin D (25(OH)D) levels, together or separately, are indicators of the risk of stroke. Materials and Methods. This prospective study was performed at two centers. In the study, 100 patients diagnosed with acute ischemic stroke and 100 control individuals in the same age range were examined. In addition to neurological examination, cranial imaging, extensive routine blood chemistry, PTH, and 25(OH)D levels were evaluated in all cases. Stroke risk factors were determined. Logistic regression was used for statistical analysis. Results. A total of 60 patients and 79 control individuals were included in the study. Different estimation models were designed in order to examine the relationship between PTH and 25(OH)D levels with stroke. According to modeling results, it was determined that the most effective predictor for risk of stroke was 25(OH)D levels, followed by hypertension and PTH levels, respectively. Conclusion. PTH and 25(OH)D levels together can make important contributions to determination of stroke risk, and further investigations are needed to understand this relationship more fully.

Concomitant Rash and Blood Dyscrasias in Geriatric Psychiatry Patients Treated with Carbamazepine

1998 ◽  
Vol 32 (9) ◽  
pp. 884-887 ◽  
Author(s):  
Marshall Cates ◽  
Richard Powers
Keyword(s):  
Blood Count ◽  
Geriatric Patients ◽  
Complete Blood Count ◽  
Case Reports ◽  
Signs And Symptoms ◽  
Geriatric Psychiatry ◽  
Drug Induced ◽  
Carbamazepine Therapy ◽  
A Prospective Study ◽  
The Relationship

BACKGROUND: Rashes and blood dyscrasias are disconcerting adverse effects associated with carbamazepine therapy. Rashes are quite common, as are mild blood dyscrasias, such as mild leukopenias. Fortunately, severe rashes and blood dyscrasias are rare. There are few reports on the relationship between carbamazepine-induced rashes and blood dyscrasias, including a prospective study in which rash appeared concomitantly with leukopenia and/or thrombocytopenia in 10 patients, two case reports in which simultaneous rash and agranulocytosis occurred, and two case reports in which rashes served as harbingers of fatal aplastic anemia. CASE REPORTS: We report two cases of concomitant rashes and blood dyscrasias in geriatric psychiatry patients receiving carbamazepine therapy for bipolar disorder. One patient was found to have a severe leukopenia within several days after rash onset. The other patient was discovered to have a severe leukopenia and thrombocytopenia within about a month after rash onset. DISCUSSION: Current hematologic monitoring guidelines for carbamazepine rely heavily on the recognition of signs and symptoms of blood dyscrasias by clinicians and patients. We believe that our cases support the suggestion that patients who develop rashes receive more vigilant monitoring of the complete blood count, should carbamazepine therapy be continued. Given the currently available case reports and the fact that the incidence of drug-induced blood dyscrasias increases with advanced age, this recommendation may be particularly relevant for geriatric patients. CONCLUSIONS: Further study is required to establish whether carbamazepine-induced concomitant rashes and blood dyscrasias are valid associations insofar as monitoring is concerned.

scholarly journals Molecular and Immune Biomarkers for Cutaneous Melanoma: Current Status and Future Prospects

Cancers ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 3456 ◽  
Author(s):  
Lorenzo Pilla ◽  
Andrea Alberti ◽  
Pierluigi Di Mauro ◽  
Maria Gemelli ◽  
Viola Cogliati ◽  
...  
Keyword(s):  
Molecular Basis ◽  
Predictive Biomarkers ◽  
Therapeutic Interventions ◽  
Current Status ◽  
Immune Biomarkers ◽  
Clinical Responses ◽  
Melanoma Patients ◽  
Immunological Assays ◽  
The Relationship

Advances in the genomic, molecular and immunological make-up of melanoma allowed the development of novel targeted therapy and of immunotherapy, leading to changes in the paradigm of therapeutic interventions and improvement of patients’ overall survival. Nevertheless, the mechanisms regulating either the responsiveness or the resistance of melanoma patients to therapies are still mostly unknown. The development of either the combinations or of the sequential treatment of different agents has been investigated but without a strongly molecularly motivated rationale. The need for robust biomarkers to predict patients’ responsiveness to defined therapies and for their stratification is still unmet. Progress in immunological assays and genomic techniques as long as improvement in designing and performing studies monitoring the expression of these markers along with the evolution of the disease allowed to identify candidate biomarkers. However, none of them achieved a definitive role in predicting patients’ clinical outcomes. Along this line, the cross-talk of melanoma cells with tumor microenvironment plays an important role in the evolution of the disease and needs to be considered in light of the role of predictive biomarkers. The overview of the relationship between the molecular basis of melanoma and targeted therapies is provided in this review, highlighting the benefit for clinical responses and the limitations. Moreover, the role of different candidate biomarkers is described together with the technical approaches for their identification. The provided evidence shows that progress has been achieved in understanding the molecular basis of melanoma and in designing advanced therapeutic strategies. Nevertheless, the molecular determinants of melanoma and their role as biomarkers predicting patients’ responsiveness to therapies warrant further investigation with the vision of developing more effective precision medicine.

Potassium as a respiratory signal in humans

1990 ◽  
Vol 69 (5) ◽  
pp. 1799-1803 ◽  
Author(s):  
C. G. Newstead ◽  
G. C. Donaldson ◽  
J. R. Sneyd
Keyword(s):  
Minute Ventilation ◽  
Blood Chemistry ◽  
Carbon Dioxide Production ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
Arterial Blood ◽  
Respiratory Signal ◽  
Carbon Dioxide Production Rate ◽  
The Relationship ◽  
Resting Muscle

Six renal transplant recipients underwent a series of incremental exercise experiments. Minute ventilation (VE), carbon dioxide production rate (VCO2), and arterial blood chemistry were measured at rest and while subjects exercised on a stationary bicycle. Four of the subjects performed a similar experiment while exercising on a static rowing machine. Within each subject, arterial potassium concentration ([K+]a) was linearly related to VCO2 and VE during exercise. The slope of the relationship between [K+]a and VCO2 was similar in the cycling and rowing experiments. This implies that the absorption of potassium by resting muscle does not significantly limit the arterial hyperkalemia seen during exercise. When VE, VCO2, and [K+]a were measured 1 and 5 min after the end of cycling there was no correlation, whereas VE continued to be closely correlated with VCO2. The relationship demonstrated between change in [K+]a and VCO2 in these experiments is compatible with change of [K+]a acting as a respiratory signal during exercise but not during recovery from exercise in humans.

scholarly journals Estimation of quality of experience (QoE) in e-Health ecosystems

2017 ◽  
Vol 37 (2) ◽  
pp. 52-59 ◽  
Author(s):  
Veronica Alexandra Rojas-Mendizabal ◽  
Arturo Serrano-Santoyo ◽  
Roberto Conte-Galvan ◽  
Salvador Villarreal-Reyes ◽  
Raul Rivera-Rodriguez
Keyword(s):  
Human Factors ◽  
Quality Of Experience ◽  
Complexity Science ◽  
Health Interventions ◽  
Ultrasound Probe ◽  
Remote Diagnosis ◽  
Satellite Link ◽  
Comprehensive Manner ◽  
The Relationship

This article proposes a framework to design and implement e- Health interventions in a comprehensive manner. We draw on complexity science to study the interplay of the ecosystem, the behavior and interactions among its agents. We provide a platform to estimate the Quality of Experience (QoE) to assess the relationship between technology and human factors involved in e-Health projects. Our aim is to estimate QoE in e-Health ecosystems from the perspective of complexity by adopting a methodology that uses fuzzy logic to study the behavior of the ecosystem’s agents. We apply the proposed framework to a remote diagnosis case by means of an ultrasound probe through a satellite link. Despite the ambiguities for determining QoE, the experiment demonstrates the applicability of the framework and allows to stressing the importance of human factors in the implementation of e-Health projects.

scholarly journals Peritoneal fluid changes in horses subjected to small colon distension

2011 ◽  
Vol 31 (5) ◽  
pp. 367-373 ◽  
Author(s):  
Rafael R Faleiros ◽  
Delphim G Macoris ◽  
Carlos Henrique C Saquetti ◽  
Andrea C Aita ◽  
Anderson Farias ◽  
...  
Keyword(s):  
Total Protein ◽  
Peritoneal Fluid ◽  
Clinical Signs ◽  
Experimental Period ◽  
Blood Chemistry ◽  
Control Group ◽  
Colon Obstruction ◽  
Before And After ◽  
Colon Distension ◽  
Biochemical Analyses

Intestinal devitalization in cases of small colon obstruction may be difficult to detect based only in clinical signs. The purpose was to serially evaluate blood and peritoneal fluid of horses subjected to small colon distension. Seventeen adult horses were allotted in three groups. In the small colon-distended group (DG, n=7) a surgically-implanted latex balloon was inflated to promote intraluminal small colon distension. In the shamoperated group (SG, n=5), the balloon was implanted but not inflated, and no surgery was done in the control group (CG, n=5). Blood and peritoneal fluid were sampled before and after (6 samples with a 30-minute interval) intestinal obstruction for cytological and biochemical analyses. No significant changes in clinical signs occurred within groups or across time during the experimental period. There were no statistical differences among SG and SG groups in hematologic and blood chemistry variables. Although total protein concentration and lactate dehydrogenase (LDH) activity in peritoneal fluid remained most of the time within reference values during the experimental period in all groups, increases from baseline values were detected in SG and DG groups. Such increases occurred earlier, progressively and with greater magnitude in the DG when compared with the SG (P<0.05). Increases from baselines values were also observed in total nucleated cells and neutrophils counts in the DG (P<0.05). In conclusion, distension of the equine small colon induced progressive subtle increases in total protein and LDH concentrations in the peritoneal fluid during the first hours. Serial evaluation of these variables in peritoneal fluid may be useful for early detection of intestinal devitalization in clinical cases of equine small colon obstruction.

scholarly journals ATIM-35. THE ASSOCIATIONS BETWEEN TOTAL LYMPHOCYTE COUNTS AFTER CONCOMITANT CHEMORADIATION WITH OVERALL SURVIVAL IN PATIENTS WITH NEWLY DIAGNOSED GLIOBLASTOMA

2019 ◽  
Vol 21 (Supplement_6) ◽  
pp. vi9-vi9
Author(s):  
Stephen Ahn ◽  
Jae-Sung Park ◽  
Yong Kil Hong ◽  
Seung Ho Yang ◽  
Sin-Soo Jeun
Keyword(s):  
Overall Survival ◽  
Complete Blood Count ◽  
Malignant Tumors ◽  
P Value ◽  
Newly Diagnosed ◽  
Total Lymphocyte ◽  
Concomitant Chemoradiation ◽  
Newly Diagnosed Glioblastoma ◽  
The Relationship

Abstract Several studies have been conducted to determine the relationship between post-treatment total lymphocyte count (TLC) and overall survival (OS) in patients with malignant tumors including glioblastomas (GBMs). In this retrospective study, whether patients with newly diagnosed GBM experience significant lymphopenia after concomitant chemoradiation (CCRT) was evaluated, and whether TLC after this treatment is associated with OS in the treated population was examined. Using electronic medical records, all patients newly diagnosed with GBM between 2008 and 2016 at Seoul St. Mary’s Hospital were retrospectively examined. The eligible criteria included the following: 1) craniotomy with surgical resection or biopsy, 2) completion of CCRT, 3) accessible baseline and/or follow-up complete blood count (CBC). Median TLC significantly decreased after completion of CCRT, compared to TLC at baseline (1,742 versus 1,319 cells/mm3, P-value &lt; 0.001). Patients with TLC &lt; 1,200 cells/mm3 at 4 weeks after the completion of CCRT showed shorter survival than those with TLC ≥ 1,200 cells/mm3 with median OS of 14.5 versus 21.0 months (P-value = 0.017). Also, in multivariate analysis for OS, TLC &lt; 1,200 cells/mm3 at 4 weeks after the completion of CCRT (HR 1.97, 95% CI 1.61 – 2.25, P-value = 0.004) were significantly associated with shorter survival. The results from the present study indicate that treatment-related total lymphocyte counts after CCRT is associated with worse survival in patients with newly diagnosed GBM.

scholarly journals Rethinking Lupus Nephritis Classification on a Molecular Level

2019 ◽  
Vol 8 (10) ◽  
pp. 1524 ◽  
Author(s):  
Salem Almaani ◽  
Stephenie D. Prokopec ◽  
Jianying Zhang ◽  
Lianbo Yu ◽  
Carmen Avila-Casado ◽  
...  
Keyword(s):  
Lupus Nephritis ◽  
Mrna Abundance ◽  
Paired Samples ◽  
Galectin 3 ◽  
Histologic Evaluation ◽  
Clinical Responses ◽  
Molecular Phenotypes ◽  
Secreted Phosphoprotein 1 ◽  
The Relationship

The International Society of Nephrology/Renal Pathology Society (ISN/RPS) lupus nephritis (LN) classification is under reconsideration, given challenges with inter-rater reliability and resultant inconsistent relationship with treatment response. Integration of molecular classifiers into histologic evaluation can improve diagnostic precision and identify therapeutic targets. This study described the relationship between histological and molecular phenotypes and clinical responses in LN. Renal compartmental mRNA abundance was measured in 54 biopsy specimens from LN patients and correlated to ISN/RPS classification and individual histologic lesions. A subset of transcripts was also evaluated in sequential biopsies of a separate longitudinal cohort of 36 patients with paired samples obtained at the time of flare and at follow up. Unsupervised clustering based on mRNA abundance did not demonstrate a relationship with the (ISN/RPS) classification, nor did univariate statistical analysis. Exploratory analyses suggested a correlation with individual histologic lesions. Glomerular FN1 (fibronectin), SPP1 (secreted phosphoprotein 1), and LGALS3 (galectin 3) abundance correlated with disease activity and changed following treatment. Exploratory analyses suggested relationships between specific transcripts and individual histologic lesions, with the important representation of interferon-regulated genes. Our findings suggested that the current LN classification could be refined by the inclusion of molecular descriptors. Combining molecular and pathologic kidney biopsy phenotypes may hold promise to better classify disease and identify actionable treatment targets and merits further exploration in larger cohorts.

The Relationship Between the Gensini Score and Complete Blood Count Parameters in Coronary Artery Disease

2012 ◽  
Vol 15 (2) ◽  
pp. 51-54 ◽  
Author(s):  
Muhammet Raşit Sayın ◽  
Mehmet Ali Çetiner ◽  
Turgut Karabağ ◽  
Sait Meut Doğan ◽  
Mustafa Aydın ◽  
...  
Keyword(s):  
Coronary Artery Disease ◽  
Coronary Artery ◽  
Blood Count ◽  
Complete Blood Count ◽  
Gensini Score ◽  
Artery Disease ◽  
The Relationship
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