120 Effects of antral follicle count in ovaries on follicular development and endocrine dynamics of follicle-stimulating hormone and steroid hormones in cattle

2019 ◽  
Vol 31 (1) ◽  
pp. 185
Author(s):  
K. Sakaguchi ◽  
Y. Yanagawa ◽  
K. Yoshioka ◽  
T. Suda ◽  
K. Kawano ◽  
...  
Keyword(s):  
Steroid Hormones ◽  
Prostaglandin F2α ◽  
Antral Follicle ◽  
Antral Follicle Count ◽  
Gonadotropin Releasing Hormone ◽  
Oestrous Cycle ◽  
Developmental Competence ◽  
Releasing Hormone ◽  
Selection Phase ◽  
Follicular Fluids

The antral follicle count (AFC) in mammalian ovaries positively correlates with female fertility. We have reported previously that cumulus-oocyte complexes derived from high-AFC ovaries produce more oestradiol-17β (E2) and have higher developmental competence than those from low-AFC ovaries in in vitro growth (Sakaguchi et al. WCRB2017, Okinawa, Japan; 1-15). We also indicated that follicular growth was different between high- and low-AFC cows, and that follicular deviation occurred earlier in high-AFC cows (Nagai et al. 2015 Anim. Reprod. Sci. 163, 172-178). To clarify the causes of the higher E2 production and earlier follicular deviation in high-AFC cows, we investigated circulating steroid hormones and FSH during the oestrous cycle, as well as steroidogenesis in follicles at different phases of the oestrous cycle. Four high-AFC cows (>40 follicles) and 3 low-AFC cows (<20 follicles) based on the peak AFC were used. We ablated their follicles 8 days after oestrus and administered prostaglandin F2α on Day 12. After 40h of prostaglandin F2α injection, follicular fluid of a dominant follicle (preovulatory phase) was collected and subordinate follicles were ablated. Gonadotropin-releasing hormone was then injected to induce LH surge. Seven days after gonadotropin-releasing hormone, follicular fluids were collected from dominant follicles (luteal phase) and all follicles were ablated. Four days later, follicular fluids were again collected from the largest follicles (selection phase). Steroid hormones in follicular fluids were measured by enzyme immunoassay. Throughout the study, we daily examined ovaries by ultrasonography and collected blood for hormone measurements. We analysed data using two-way ANOVA followed by Tukey-Kramer or Student’s t test. The number of small follicles (<4mm) was greater in high-AFC than low-AFC groups (low v. high=7.6v. 46.9; P<0.05), whereas that of large follicles (>8mm) was similar between groups (0.8v. 0.8) at any time. Although the number of medium-sized follicles (4-8mm) was not affected in the low-AFC group (4.2-6.7) after follicle ablation, the number increased from 1 to 4 days (10.5 to 17.8) then decreased 6 days after follicle ablation (11.5) in the high-AFC group, which means that degradation of follicles occurs at selection phase in high-AFC cows. Peripheral FSH concentration was higher (low v. high: 4.7v. 2.1 ng/mL), but E2 concentration was lower in the low-AFC (2.4 pg/mL) than high-AFC group (3.3 pg/mL) during the selection phase (P<0.05). Although follicular diameter was similar in both groups at any phase, E2 (low v. high=333.8v. 1127.4 ng/mL; P<0.05) and testosterone (low v. high=16.7v. 35.0 ng/mL; P=0.07), but not progesterone, concentrations were higher in the high-AFC than low-AFC group in the preovulatory phase. These findings suggest that a lower response to FSH causes low E2 production in the low-AFC group, resulting in high FSH concentration and a consistent development of medium-sized follicles. Conversely, higher E2 concentration suppresses FSH secretion, resulting in obvious degradation of follicles in the high-AFC group at the selection phase.

252 EFFECT OF SUPERSTIMULATORY TREATMENT TO DONOR COWS IN OXYGEN CONSUMPTION OF MATURED OOCYTES

2013 ◽  
Vol 25 (1) ◽  
pp. 273
Author(s):  
K. Imai ◽  
S. Sugimura ◽  
M. Ohtake ◽  
Y. Aikawa ◽  
Y. Inaba ◽  
...  
Keyword(s):  
Oxygen Consumption ◽  
Prostaglandin F2α ◽  
Gonadotropin Releasing Hormone ◽  
Oestrous Cycle ◽  
Control Group ◽  
Releasing Hormone ◽  
And Control ◽  
Bovine Oocytes

We previously reported that follicular wave synchronization and follicular growth treatment (FGT) before ovum pick-up (OPU) were effective in improving oocyte competence, which was associated with an increase in related embryos obtained by somatic cell nuclear transfer (Sugimura et al. 2012 Cell. Reprogram. 14, 29–37). However, oxygen consumption in oocytes remained unknown. The present study was designed to examine the differences in oxygen consumption between bovine oocytes obtained by OPU with or without FGT after in vitro maturation. Holstein dry cows (n = 8) were reared under the same feeding and environmental conditions. Two OPU sessions were conducted in each cow to collect immature oocytes, as described by Sugimura et al. (2012). The first OPU session (OPU group) was performed in cows on arbitrary days of the oestrous cycle, using a 7.5-MHz linear transducer with the needle connected to an ultrasound scanner. Follicles larger than 8 mm in diameter were then aspirated and a controlled internal drug release device (CIDR) was inserted on Day 5 (the day of the first OPU session = Day 0). Then 30 Armour units (AU) of FSH (Antrin, Kyoritsu Seiyaku, Tokyo, Japan) was administrated to cows twice a day from Day 7 to 10 in decreasing doses (6, 6, 4, 4, 3, 3, 2, 2 AU day–1). Cloprostenol (prostaglandin F2α; 0.75 mg) was administered in the morning of Day 9. The second OPU session (FGT-OPU group) was performed 48 h after prostaglandin F2α administration (Day 11), and only follicles larger than 5 mm in diameter were aspirated. The CIDR was removed from the cows just before OPU. Collected cumulus–oocyte complexes in the OPU and FGT-OPU groups were matured in vitro as described by Imai et al. [2006 J. Reprod. Dev. 52(Suppl.), S19–S29]. To collect in vivo-matured oocytes (control group), the CIDR was inserted into the cows on arbitrary days of the oestrous cycle (= Day 0), and oestradiol benzoate (0.8 mg) was administered on Day 1. The cows received the FGT treatment (as described above) from Day 6 to 10; however, the CIDR was removed in the evening of Day 8. Buserelin (gonadotropin-releasing hormone; 200 µg) was then administrated in the morning of Day 10, and OPU was performed at 24 h after gonadotropin-releasing hormone administration (Day 11). Oxygen consumption of matured oocytes was measured noninvasively with a scanning electron microscopy system (HV-405SP; Hokuto Denko Co., Tokyo, Japan). Data were analysed by ANOVA followed by a Tukey-Kramer test. There was no difference in the mean oxygen consumption between the FGT-OPU group (0.34 ± 0.02 × 10–14 mol–1, mean ± SEM) and control group (0.40 ± 0.01 × 10–14 mol–1). However, oxygen consumption in the FGT-OPU and control groups was significantly lower (P < 0.01) than that in the OPU group (0.50 ± 0.02 × 10–14 mol–1). These results revealed significantly lower oxygen consumption in OPU-derived in vitro-matured bovine oocytes after FGT treatment compared with those obtained without FGT treatment. Oxygen consumption of oocytes obtained from FGT-OPU was similar to that of in vivo-matured oocytes, which may reflect their cytoplasmic maturation status with high developmental competence.

142 EFFECT OF HIGH AND LOW ANTRAL FOLLICLE COUNT IN PUBERTAL BEEF HEIFERS ON IVF

2014 ◽  
Vol 26 (1) ◽  
pp. 184
Author(s):  
C. C. Chase ◽  
E. C. Wright ◽  
A. K. McNeel ◽  
R. A. Cushman ◽  
G. A. Perry ◽  
...  
Keyword(s):  
Prostaglandin F2α ◽  
Antral Follicle ◽  
Antral Follicle Count ◽  
Blastocyst Stage ◽  
Oestrous Cycle ◽  
Blastocyst Development ◽  
Maturation Medium ◽  
Frozen Semen ◽  
Oocyte Collection ◽  
Collection Medium

Pubertal heifers can be classified between those with high (n = 25) or low (n = 15) antral follicle counts (AFC). The objective of this study was to determine oocyte development and maturation (e.g. fertility) in an IVF system for high- and low-AFC heifers. From a pool of 120 heifers, 10 high- and 10 low-AFC heifers were determined by transrectal ultrasonography; all heifers with evidence of oestrous cyclicity (i.e. pubertal) were synchronized with two 5-mL injections of prostaglandin F2α 11 days apart. Heifers were euthanized over 4 days on Days 15 to 16 of the synchronized oestrous cycle. A total of 15 heifers (n = 7 high and n = 8 low AFC) were at the appropriate stage of the oestrous cycle. Ovaries were collected and transported to the laboratory. Follicles less than 8 mm in diameter were aspirated. The IVF procedures and media were as previously described (Miles et al. 2004. Biol. Reprod. 71, 1919–1926). Cumulus-oocyte complexes (COC) were identified and washed in oocyte collection medium and then in maturation medium and were cultured (5% CO2; 38.5°C) for 24 h. Following maturation, COC were transferred and washed in fertilization medium. Thawed frozen semen from a crossbred bull was subjected to the swim-up procedure. Motile spermatozoa were collected and added to COC to yield a final concentration of spermatozoa per milliliter of fertilization medium. About 24 h later, presumptive zygotes were washed in development medium, placed in microdrops of development medium, and cultured for 8 days. On Days 3 and 8 after fertilization, cleavage and blastocyst development, respectively, were assessed. Data were analysed using the Proc Mixed procedure of SAS (SAS Institute Inc., Cary, NC, USA) and the model included the effects of day of collection (n = 4), group (n = 7 high- or n = 8 low-AFC heifers), and the interaction. The interaction did not differ (P = 0.10). Day of collection influenced (P < 0.05) the number of COC and the number of oocytes cleaved. High- compared to low-AFC heifers had the greater (P < 0.05) numbers of COC (42.7 ± 4.66 v. 22.1 ± 4.59), oocytes that cleaved (28.1 ± 3.60 v. 15.9 ± 3.55), and developed to blastocysts (13.2 ± 1.71 v. 6.2 ± 1.69). However, there was no difference (P > 0.10) in the percentage of COC that cleaved (65.3 ± 5.58 v. 66.2 ± 5.50%, high v. low, respectively) or that developed to blastocysts (46.7 ± 6.75 v. 42.2 ± 6.65%). In conclusion, AFC did not appear to affect oocyte maturation and development through the blastocyst stage.

181 IN VITRO FERTILIZATION (IVF) USING SEMI-DEFINED CULTURE CONDITIONS FROM LOW OR HIGH ANTRAL FOLLICLE COUNT PUBERTAL BEEF HEIFERS

2016 ◽  
Vol 28 (2) ◽  
pp. 221
Author(s):  
C. C. Chase ◽  
R. A. Cushman ◽  
A. K. McNeel ◽  
O. L. Amundson ◽  
G. A. Perry ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Prostaglandin F2α ◽  
Antral Follicle ◽  
Antral Follicle Count ◽  
Oestrous Cycle ◽  
Vitro Fertilization ◽  
Development Rates ◽  
Frozen Semen ◽  
Defined Culture

To compare the in vitro fertilization (IVF) and production (IVP) of embryos from low and high antral follicle count (AFC) heifers, AFC were determined on 106 heifers using transrectal ultrasonography. Ten heifers with the lowest AFC (avg. 13.2) and 10 heifers with the highest AFC (avg. 27.4) with evidence of oestrous cyclicity were synchronised with 2 injections of prostaglandin F2α (PGF2α); half were harvested on Days 5 to 6 and half on Days 15 to 16 of the oestrous cycle. The IVF procedures included protocols for semi-defined media and were as described (IVP Protocol, P. J. Hansen’s Laboratory, University of Florida, Gainesville, FL, USA). Cumulus-oocyte complexes (COC) from follicles less than 8 mm in diameter were cultured in maturation medium (5% CO2; 38.5°C) for 24 h. Matured COC were fertilised using thawed frozen semen from a crossbred bull that was purified using Percoll gradient separation procedures. Motile spermatozoa were added to COC in fertilization medium at a final concentration of 1 × 106 spermatozoa per mL. About 24 h later, presumptive zygotes were placed in microdrops of development medium under oil, and cultured (5% CO2, 5% O2, balance N2, 38.5°C). On Days 3 and 8 after fertilization, cleavage and blastocyst development rates, respectively, were assessed. Data were analysed using the mixed procedure of SAS (SAS Institute, Cary, NC, USA) and the model included the effects of collection day, group (high or low AFC), and their interaction. More COC (P < 0.0005) were collected from high than low AFC heifers (30.3 v. 9.3 ± 3.12 per heifer). Both the number and percentage of COC that cleaved had an interaction between collection day and group (P < 0.03). The interaction appeared to be due to low cleavage and development rates on 1 of 4 collection days (appeared not related to day of oestrous cycle). Although high compared to low AFC heifers had more COC that cleaved (18.7 v. 4.4 ± 1.84 per heifer), the percentage of cleaved COC did not differ (59.2 v. 49.8 ± 3.36%). There were no significant differences between high and low AFC heifers in the number of blastocysts (3.1 v. 0.6 ± 1.21 per heifer) or in the percentage of COC that developed to blastocysts (8.8 v. 5.2 ± 3.60%). In previous replicates (years), we reported that high AFC heifers had more COC collected, more COC that cleaved, and more COC that developed to blastocysts than low AFC heifers. In contrast, in this study numbers of COC that developed to blastocysts did not significantly differ between high and low AFC heifers. Additionally, the percentage of COC that cleaved, and that developed to blastocyst have been similar between high and low AFC heifers. Therefore, high compared to low AFC heifers may produce more IVP embryos; however, AFC does not appear to affect the competence of an oocyte to develop and mature to the blastocyst stage.

121 Ovulatory response to gonadotropin-releasing hormone relative to day and diameter of dominant follicle during the first follicular wave in Beetal goats

2019 ◽  
Vol 31 (1) ◽  
pp. 186
Author(s):  
W. Ahmad ◽  
M. I.-R. Khan ◽  
A. Murtaza ◽  
I. Mohsin ◽  
A. Riaz ◽  
...  
Keyword(s):  
Growth Rate ◽  
Prostaglandin F2α ◽  
Binary Logistic Regression ◽  
Gonadotropin Releasing Hormone ◽  
Oestrous Cycle ◽  
New Wave ◽  
Dominant Follicle ◽  
Releasing Hormone ◽  
Follicular Wave ◽  
Ovulatory Follicles

In Beetal goats under subtropical conditions, ovulatory response of first-wave dominant follicle based on diameter and day of oestrous cycle is unknown to date. The objective of this study was to evaluate the ovulatory response to gonadotropin-releasing hormone (GnRH) relative to day and diameter of dominant follicle during the first follicular wave in Beetal goats. Fifty goats were synchronized using a single dose of prostaglandin F2α (75 µg; D+ cloprostenol, Fatro, Italy), and scanned every 6 hours for ovulation using a 7.5-MHz trans-rectal transducer (Honda HS: 1500, Japan). Following ovulation (Day 0), goats (n=40; 10/day) were given GnRH (25µg lecirelin, Fatro, Italy) on Days 2, 4, 6, and 8 of oestrous cycle. Follicular dynamics was monitored daily from D 0 until GnRH, and every 4 hours after GnRH until ovulation. Ovulation frequency relative to day and dominant follicular diameter was analysed by binary logistic regression, whereas diameter of dominant follicle at time of GnRH, ovulation time, time of wave emergence and growth rate of dominant follicle following GnRH were analysed by one-way ANOVA and independent sample t-test (SPSS 20, IBM Corp., Armonk, NY, USA). Eighty percent of the goats ovulated within 74.7±1.3h after prostaglandin F2α. Overall, 37% (14/38) of the goats ovulated following GnRH within 12.0±1.5h. Ovulations (%) following GnRH were higher (P=0.03) on Day 4 than 2 (60 v. 10%; i.e. 14 times higher than Day 2) and decreased gradually from Day 4 to 8 (P&gt;0.05). Similarly, ovulations following GnRH were higher (P=0.00) in first-wave dominant follicles having diameter 6.1-7.0mm than those having less than 6.1mm (88v. 23%; i.e. 24 times higher than &lt;6.1mm). Ovulations (%) decreased as the diameter of dominant follicle increased from 6.1-7.0mm (P&gt;0.05). Diameters of ovulatory follicles at time of GnRH between Day 2, 4, 6, and 8 were not different (P&gt;0.05). Regardless of day of GnRH treatment, diameter of ovulatory v. anovulatory follicles differed (6.44±0.03v. 5.1±0.4 mm; P=0.00). Overall, growth rate of dominant follicles following GnRH was 0.2±0.11mm and did not differ between ovulatory and anovulatory goats (0.29±0.13v. 0.13±0.15 mm; P&gt;0.05). Interval to the emergence of a new wave following GnRH was prolonged for Day 4 compared to Day 2, 6, and 8 (i.e. 22v. 8, 11, and 12 h; respectively, P&lt;0.05). In conclusion, GnRH treatment at Day 4 resulted in maximum ovulations as the first-wave dominant follicle reached a size of 6.1 to 7.0 mm.

Effect of gonadotropin-releasing hormone and prostaglandin F2α on reproduction in postpartum dairy cows

1983 ◽  
Vol 19 (6) ◽  
pp. 763-770 ◽  
Author(s):  
G.F. Richardson ◽  
L.F. Archbald ◽  
D.M. Galton ◽  
R.A. Godke
Keyword(s):  
Dairy Cows ◽  
Prostaglandin F2α ◽  
Gonadotropin Releasing Hormone ◽  
Releasing Hormone

Estrous synchronization and fertility of cross-bred dairy cows using prostaglandin F2α and gonadotropin releasing hormone

2021 ◽  
pp. 171-175
Author(s):  
Abubakr O. Ismail ◽  
◽  
Erneo B. Ochi ◽  
Ambrose S. Jubara ◽  
Bakri Ahmed A/Rahim ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Artificial Insemination ◽  
Prostaglandin F2α ◽  
Fixed Time ◽  
Gonadotropin Releasing Hormone ◽  
Pregnancy Rates ◽  
Releasing Hormone ◽  
Treatment Groups ◽  
Estrous Synchronization ◽  
Descriptive Statistical Analysis

A comparative hormonal study of prostaglandin, gonadotropin releasing hormone and their combination was conducted on 30 randomly selected cross-bred dairy cows of SEMEX project for artificial insemination in Hillat Kuku, Khartoum North, Sudan. The study attempts to determine the response of the animals to hormonal induction and synchronization of estrous as well as fertility following fixed time artificial insemination. Three treatment groups of 10 cows each were undertaken. Groups 1, 2 and 3 involved administration of prostaglandin F2α (PGF2α), gonadotropin releasing hormone (GnRH) and a combination of PGF2α + GnRH, respectively. Descriptive statistical analysis was performed. The results revealed that all the three protocols induced and synchronized estrous were almost synchronous in all the treated groups. However, protocol 3 revealed a significantly (p<0.05) better result of 70% pregnancy rate compared to other two protocols that equally provided 50% pregnancy rates. The study resolves that protocol 3 be highly recommended to change the mindset of farmers about the spread of reproductive technology in Sudan. Protocols 1 and 2 provided acceptable pregnancy rates that can be enhanced with the improvement of management.

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