311 RISK ANALYSIS OF ALPHA-LACTALBUMIN TRANSGENE TRANSFER TO NON-TRANSGENIC CONTROL ANIMALS DURING REARING AND BREEDING

2009 ◽  
Vol 21 (1) ◽  
pp. 252
Author(s):  
M. B. Wheeler ◽  
W. L. Hurley ◽  
S. Lane ◽  
J. Mosley ◽  
G. E. Bressner ◽  
...  
Keyword(s):  
Risk Assessment ◽  
Mammary Gland ◽  
Large Scale ◽  
Initial Step ◽  
Scientific Data ◽  
Bodily Fluids ◽  
Transgenic Livestock ◽  
Lactating Mammary Gland ◽  
Transgenic Swine

Assessment of general risk posed from transgenic (T) animals is important to their future contributions to society. Identification of potentially harmful properties of transgenic livestock is the initial step in a risk assessment. Direct and indirect impacts of potential harmful properties of T livestock need to be evaluated at 3 levels, namely (1) characterization of how the transgene, its product, and the T livestock behave in their immediate environment, that is, in their barn or pen; (2) determination of possible impacts of large-scale release of T livestock, that is, if they were to be integrated into the larger population of food animal livestock; and (3) determination of the more complex environmental and safety consequences of their release into livestock populations. We previously developed and characterized transgenic swine containing a mammary-specific transgene (bovine α-lactalbumin, bALAC) that results in increased milk production in sows. We currently are determining whether bALAC is expressed in tissues of T swine other than the lactating mammary gland and whether the transgene DNA (Tg) crosses into non-transgenic control (C) swine under various physiological and physical conditions. The specific aims addressed in the present study were to determine: (1) whether the Tg can be transferred directly from T animals to C animals by physical association or contact and (2) whether the Tg can be transferred directly from an adult T animal to an adult C animal via mating. The T animals utilized in these studies are in at least generation 10 and have stable incorporation of the Tg. Comparable age- and weight-matched animals, T and C, were housed together allowing for general contact that is normal within swine production, for either 180, 220, or 250 d of age after weaning. Swine due to their behavior ingest saliva, regurgitated food, and stool or urinary products, as well as other bodily fluids and cells during normal housing. In a second study, vaginal, cervical, uterine, oviductal, and ovarian tissues from C females on 2, 7, or 90 d after mating to T males and penis, bulbourethral gland, urethra, testis, and epididymis tissues from C males on 2 or 7 days after mating to Tg females were collected. The presence of Tg in tissues from all C animals was tested via PCR. We have analyzed for the presence of the Tg in various tissues [including mammary gland, salivary gland, skin (sebaceous gland), muscle, lung, liver, kidney, brain, ovary, oviduct, uterus, cervix, vagina, penis, bulbourethral gland, urethra, testis, epididymis, and intestine]. Results indicate no presence of the Tg in tissues of C animals (n = 28) after co-habitation for 180, 220, or 250 d (n = 305 samples analyzed) or at 2 (n = 5), 7(n = 14), or 90 (n = 2) d post-mating (n = 60, 174, or 24 samples analyzed, respectively). The present results suggest that there is no horizontal Tg transmission between T and C pigs due to rearing or mating. This work provides a critical step toward providing rigorous scientific data for risk assessment of transgenic livestock. This project supported by the USDA BRAG Project #2005-03799.

311 RISK ASSESSMENT OF α-LACTALBUMIN TRANSGENIC PIGS

2008 ◽  
Vol 20 (1) ◽  
pp. 235 ◽  
Author(s):  
M. B. Wheeler ◽  
W. L. Hurley ◽  
S. J. Lane ◽  
G. E. Bressner ◽  
T. VanEtten ◽  
...  
Keyword(s):  
Risk Assessment ◽  
Mammary Gland ◽  
Large Scale ◽  
Transgenic Animals ◽  
Initial Step ◽  
Scientific Data ◽  
Transgenic Pigs ◽  
Transgenic Livestock ◽  
Transgenic Swine

Assessment of the general risk posed by transgenic animals is important to their future contributions to society. Identification of potentially harmful properties of transgenic livestock is the initial step in a risk assessment. Direct and indirect impacts of potential harmful properties of transgenic livestock need to be evaluated at 3 levels: (1) characterization of how the transgene, the transgene product, and the transgenic livestock behave in their immediate environment; that is, in their barn or pen; (2) determination of possible impacts of large-scale release of transgenic livestock; that is, if they were to be integrated into the larger population of food animal livestock; and (3) determination of the more complex environmental and safety consequences of their release into the livestock population. We previously developed and characterized transgenic swine containing a mammary-specific transgene (bovine α-lactalbumin, bALAC) that results in increased milk production in sows (Bleck et al. 1998). We are currently determining whether bALAC is expressed in tissues of transgenic (T) swine other than the lactating mammary gland, and whether the transgene (DNA; Tg) crosses into non-transgenic control (C) swine under various physiological and physical conditions. The specific aims addressed in the present study were to determine (1) whether the Tg can be transferred directly from T animals to C animals by physical association or contact, and (2) whether the Tg can be transferred directly from an adult T animal to an adult C animal via mating. The T animals utilized in these studies were in generation 10 at least and have stable incorporation of the Tg. Comparable age- and weight-matched animals, T and C, were housed together allowing for general contact that is normal within swine production, for 180, 220, or 250 days after weaning. Due to the nature of swine behavior, these animals may ingest saliva, regurgitated food, and stool and urinary products as well as other bodily fluids and cells during normal housing and establishment of dominance hierarchy. In a second study, vaginal, cervical, uterine, oviductal, and ovarian tissues were collected from C females on 2 or 7 days after mating to T males. The presence of Tg in tissues from all C animals was tested via PCR. We have analyzed for the presence of the Tg in various tissues, including mammary gland, salivary gland, skin (sebaceous gland), muscle, lung, liver, kidney, brain, ovary, oviduct, uterus, cervix, vagina, and intestine. Preliminary results indicate no presence of the Tg in tissues of C animals (n = 20) after cohabitation for 180, 220, or 250 days (n = 201 samples analyzed) or at 2 (n = 3) or 7 (n = 5) days post-mating (n = 38 and 59 samples analyzed, respectively). This work provides a critical first step toward providing rigorous scientific data for risk assessment of transgenic livestock. The USDA BRAG Program, Project No. 2005–03799, supported this work.

436 RISK ANALYSIS OF α-LACTALBUMIN TRANSGENE TRANSFER TO NONTRANSGENIC CONTROL ANIMALS DURING REARING, BREEDING, PARTURITION AND LACTATION

2010 ◽  
Vol 22 (1) ◽  
pp. 375 ◽  
Author(s):  
M. B. Wheeler ◽  
W. L. Hurley ◽  
J. Mosley ◽  
G. E. Bressner ◽  
E. Monaco ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Close Contact ◽  
Initial Step ◽  
Swine Production ◽  
Physical Conditions ◽  
Bodily Fluids ◽  
Transgenic Livestock ◽  
Lactating Mammary Gland ◽  
Placental Membranes ◽  
Transgenic Swine

Assessment of general risk posed from transgenic (T) animals is important to their future contributions to society. Identification of potentially harmful properties of transgenic livestock is the initial step in a risk assessment. We previously developed and characterized transgenic swine containing a mammary-specific transgene (bovine a-lactalbumin, bALAC) that results in increased milk production in sows. We are currently determining whether bALAC is expressed in tissues of T swine other than the lactating mammary gland and whether the transgene DNA (Tg) crosses into nontransgenic control (C) swine under various physiological and physical conditions. The specific aims addressed in the present study were to determine (1) whether the Tg can be transferred directly by physical association or contact; (2) whether the Tg can be transferred directly via mating; (3) whether the Tg can be transferred directly during gestation and parturition; and (4) whether the Tg can be transferred directly during lactation. The T animals utilized in these studies are in at least generation 10 and have stable incorporation of the Tg. Comparable age- and weight-matched animals, T and C, were housed together allowing general contact that is normal in swine production, for either 180, 220, or 250 days of age after weaning. Swine typically ingest saliva, regurgitated food, and stool or urinary products, as well as other bodily fluids and cells during normal housing. In the second study, vaginal, cervical, uterine, oviductal, and ovarian tissues from C females on 2, 7, or 90 days after mating to T males, and penis, bulbourethral gland, urethra, testis, and epididymis tissues from C males on 2 or 7 days after mating to Tg females were collected. The presence of Tg in tissues from all C animals was tested by using PCR. We have analyzed for the presence of the Tg in various tissues [including mammary gland, salivary gland, skin (sebaceous gland), muscle, lung, liver, kidney, brain, ovary, oviduct, uterus, cervix, vagina, penis, bulbourethral gland, urethra, testis, epididymis, blood, inner and outer placental membranes and intestine]. Results indicate no presence of the Tg in tissues of C animals (n = 28) after co-habitation for 180, 220, or 250 days (n = 305 samples analyzed) or at 2 (n = 7), 7 (n = 16), or 90 (n = 6) days post-mating (n = 72, 192, or 71 samples analyzed, respectively). At Day 112 of gestation, all the samples (n = 78 samples analyzed) from nontransgenic piglets (n = 13) whose dam was aTg female were negative except for the outer placental membrane (n = 13), which screened positive for the transgene. This is not surprising because the outer placental membrane is in close contact with the uterus of the Tg dam. Finally, control piglets (n = 4) that were cross-fostered (3 days after birth) and suckled Tg dams showed no evidence of the transgene in their tissues (n = 20 samples analyzed) at weaning. The present results suggest that there is no horizontal Tg transmission between T and C pigs caused by rearing, mating, gestation, or lactation. This project was supported by USDA BRAG Project #2005-03799.

Toward Risk Assessment of Explosion Hazard: Experimental Determination of Flame Fractal Dimension

2011 ◽  
Vol 320 ◽  
pp. 151-155 ◽  
Author(s):  
Daisuke Takeda ◽  
Woo Kyung Kim ◽  
Yukari Wada ◽  
Kazunori Kuwana ◽  
Toshio Mogi ◽  
...  
Keyword(s):  
Risk Assessment ◽  
Fractal Dimension ◽  
Large Scale ◽  
Previous Method ◽  
Small Scale ◽  
Explosion Hazard ◽  
Scale Experiment ◽  
The Cost ◽  
Explosion Experiment

Quantitative risk analysis is a method to evaluate risk and to identify areas for risk reduction. The final goal of our study is to propose an effective method for risk assessment of explosion hazard. To achieve the goal, a phenomenon that influences the consequences of explosion is first identified: self-turbulization and resulting acceleration of expanding flame during explosion. The fractal dimension is then identified as the key parameter that characterizes the phenomenon. Since the previous method to determine fractal dimension relies on large-scale explosion experiment, it has not been easy to determine fractal dimension. This paper demonstrates the possibility of determining fractal dimension by analyzing flame images of small-scale experiment, which might significantly reduce the cost of risk assessment of explosion hazard.

Antibody production in milk serum after antigen instillation of the goat mammary gland. IX. Sham infection studies with Neisseria meningitidis L.C.D.C. 608B

1975 ◽  
Vol 21 (5) ◽  
pp. 662-667 ◽  
Author(s):  
Arthur E. Pasieka ◽  
G. Calver ◽  
C. P. Kenny ◽  
C. Perusse ◽  
L. F. Guerin ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Neisseria Meningitidis ◽  
Large Scale ◽  
Analytical Ultracentrifugation ◽  
Gel Filtration ◽  
Goat Milk ◽  
Scale Production ◽  
Strain Isolation ◽  
Lactating Mammary Gland ◽  
Immune Globulins

Specific immune globulins have been prepared in goat milk in response to the intramammary gland instillation of Neisseria meningitidis 608, serogroup B strain. Isolation, purification, and characterization of the goat whey by gel filtration, electrophoresis, and analytical ultracentrifugation demonstrated that the active immune component resided in the IgA class of globulins, specifically 9.2-S IgA. The potential of the lactating mammary gland as a "biological factory" for the large-scale production of diagnostic antiserum to killed bacterial whole cell antigen is described.

Large-scale Motion of Solar Filaments

2000 ◽  
Vol 179 ◽  
pp. 205-208
Author(s):  
Pavel Ambrož ◽  
Alfred Schroll
Keyword(s):  
Magnetic Flux ◽  
Proper Motion ◽  
Time Scale ◽  
Large Scale ◽  
Accuracy Of Measurements ◽  
Solar Filaments ◽  
General Movement ◽  
Scale Motion ◽  
Velocity Scatter

AbstractPrecise measurements of heliographic position of solar filaments were used for determination of the proper motion of solar filaments on the time-scale of days. The filaments have a tendency to make a shaking or waving of the external structure and to make a general movement of whole filament body, coinciding with the transport of the magnetic flux in the photosphere. The velocity scatter of individual measured points is about one order higher than the accuracy of measurements.

EFFECT OF LOCALLY IMPLANTED OESTROGEN ON THE RESPONSE OF THE RAT'S LACTATING MAMMARY GLAND TO OXYTOCIN

1973 ◽  
Vol 73 (4) ◽  
pp. 700-712 ◽  
Author(s):  
J. D. Bruce ◽  
X. Cofre ◽  
V. D. Ramirez
Keyword(s):  
Mammary Gland ◽  
Mammary Glands ◽  
Myoepithelial Cells ◽  
Recording System ◽  
Saline Infusion ◽  
High Dose ◽  
Low Doses ◽  
Milk Ejection ◽  
Lactating Mammary Gland ◽  
Small Rise

ABSTRACT On the day following delivery (day 1 of lactation) one abdominal mammary gland was implanted with oestrogen and the contralateral gland received an empty needle. At 2, 5 or 10 days of lactation the rats were anaesthetized with pentobarbital and the nipples of both abdominal glands were cannulated and their pressures recorded by means of transducers coupled to an amplifier and recording system. The normal mammary glands of 5-day lactating rats responded to very low doses of oxytocin (Syntocinon®, Sandoz) (5× 10−8 mU) with a rhythmic elevation in pressure. However, saline infusion also evoked a small rise in intra-mammary pressure. Earlier (2 days) and later (10 days) in lactation the responses were smaller. Oestrogen decreases significantly the milk ejection response to oxytocin, and the effect was maximal at day 10 of lactation. Histological observations confirmed the diminished reaction of the gland to oxytocin, since the milk was retained in the alveoli of rats bearing a mammary-oestrogen implant. A paradoxical rise in pressure was detected in normal as well as in oestrogen-implanted glands when the lowest dose of oxytocin was injected in lactating rats which had previously received a high dose of oxytocin (50 mU or 500 mU). These results reinforce the hypothesis that oestrogen alters the milk ejection response to oxytocin and that the mechanism is probably related to changes in the contractility of the myoepithelial cells.

ÜBER DIE WIRKUNG PHARMAKOLOGISCHER OXYTOCINDOSEN AUF DIE MILCHDRÜSE

1960 ◽  
Vol XXXIV (IV) ◽  
pp. 543-557 ◽  
Author(s):  
B. Berde ◽  
A. Cerletti
Keyword(s):  
Mammary Gland ◽  
Intravenous Administration ◽  
Intravenous Infusion ◽  
Intranasal Administration ◽  
Intramuscular Injection ◽  
Different Types ◽  
Lactating Mammary Gland ◽  
Synthetic Oxytocin

ABSTRACT A study was made of the influence of pharmacological amounts of synthetic oxytocin (»Syntocinon«) on the lactating mammary gland of the rabbit. The drug was given by intravenous infusion, by intramuscular injection and by intranasal administration. Two different types of reaction were noted: a tonic reaction, i. e. a lasting increase in pressure in the mammary gland without significant fluctuations, or a rhythmic reaction, i. e. a series of increases in pressure at more or less regular intervals. In order to elicit reactions approximately identical in intensity and character with those produced by intravenous infusion, it was necessary to give approximately 1.5 to 8.0 times as much by intramuscular injection and approximately 10 to 100 times as much by intranasal administration. Intravenous administration of adrenaline transiently suppressed a long-lasting reaction to oxytocin.

Methods of risk assessment in forecasting the consequences of accidents at pipeline transport facilities

2020 ◽  
Vol 10 (6) ◽  
pp. 663-673
Author(s):  
Vladislav N. Slepnev ◽  
◽  
Alexander F. Maksimenko ◽  
Elena V. Glebova ◽  
Alla Т. Volokhina ◽  
...  
Keyword(s):  
Risk Assessment ◽  
Evaluation Method ◽  
Assessment Procedure ◽  
Expert Evaluation ◽  
Main Pipeline ◽  
Pipeline Transport ◽  
Forecasting Accuracy ◽  
Distribution Of Resources ◽  
Expert Evaluation Method

The choice of risk assessment procedure is one of the essential stages of efficient structuring of processes on prevention, localization and elimination of the consequences of accidents at main pipeline transport facilities. The authors analyzed themed publications and regulatory documents, governing procedures of risk assessment and forecasting of the consequences of possible accidents, and defined main problems in this area. Procedure for the risk assessment of accidents at main pipeline facilities was developed, the basis of which is the expert evaluation method. The procedure includes the determination of the main criteria for the assessment the probability of accident initiation and development and the evaluation of the severity of its consequences, an expert evaluation of criteria significance, their classification, and creation of a rating for hazardous pipeline sections. The application of the procedure application allows to specify the list of facilities that require high priority forecasting of accidents consequences, thus to optimize the distribution of resources and the overall increase of efficiency in planning while defining forces and special technical devices, necessary for containment and rectification of emergencies. Expert evaluation method application allows considering the specifics of certain enterprises, their technical and technological peculiarities, thereby increasing forecasting accuracy.

Methods for the Quantification of Salivary Cortisol and of a-amylase in Biosensors and Portable Devices

2018 ◽  
Vol 68 (12) ◽  
pp. 2857-2859
Author(s):  
Cristina Mihaela Ghiciuc ◽  
Andreea Silvana Szalontay ◽  
Luminita Radulescu ◽  
Sebastian Cozma ◽  
Catalina Elena Lupusoru ◽  
...  
Keyword(s):  
Real Time ◽  
Medical Practice ◽  
Salivary Cortisol ◽  
Clinical Studies ◽  
Large Scale ◽  
Psychological Research ◽  
Portable Devices ◽  
Salivary Amylase ◽  
Specificity And Sensitivity

There is an increasing interest in the analysis of salivary biomarkers for medical practice. The objective of this article was to identify the specificity and sensitivity of quantification methods used in biosensors or portable devices for the determination of salivary cortisol and salivary a-amylase. There are no biosensors and portable devices for salivary amylase and cortisol that are used on a large scale in clinical studies. These devices would be useful in assessing more real-time psychological research in the future.

Identification of N-Substituted Triazolo-azetidines as Novel Antibacterials using pDualrep2 HTS Platform

2019 ◽  
Vol 22 (5) ◽  
pp. 346-354
Author(s):  
Yan A. Ivanenkov ◽  
Renat S. Yamidanov ◽  
Ilya A. Osterman ◽  
Petr V. Sergiev ◽  
Vladimir A. Aladinskiy ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Large Scale ◽  
Underlying Mechanism ◽  
Luciferase Assay ◽  
Reporter System ◽  
E Coli ◽  
Starting Point ◽  
High Concentrations ◽  
Mic Value

Aim and Objective: Antibiotic resistance is a serious constraint to the development of new effective antibacterials. Therefore, the discovery of the new antibacterials remains one of the main challenges in modern medicinal chemistry. This study was undertaken to identify novel molecules with antibacterial activity. Materials and Methods: Using our unique double-reporter system, in-house large-scale HTS campaign was conducted for the identification of antibacterial potency of small-molecule compounds. The construction allows us to visually assess the underlying mechanism of action. After the initial HTS and rescreen procedure, luciferase assay, C14-test, determination of MIC value and PrestoBlue test were carried out. Results: HTS rounds and rescreen campaign have revealed the antibacterial activity of a series of Nsubstituted triazolo-azetidines and their isosteric derivatives that has not been reported previously. Primary hit-molecule demonstrated a MIC value of 12.5 µg/mL against E. coli Δ tolC with signs of translation blockage and no SOS-response. Translation inhibition (26%, luciferase assay) was achieved at high concentrations up to 160 µg/mL, while no activity was found using C14-test. The compound did not demonstrate cytotoxicity in the PrestoBlue assay against a panel of eukaryotic cells. Within a series of direct structural analogues bearing the same or bioisosteric scaffold, compound 2 was found to have an improved antibacterial potency (MIC=6.25 µg/mL) close to Erythromycin (MIC=2.5-5 µg/mL) against the same strain. In contrast to the parent hit, this compound was more active and selective, and provided a robust IP position. Conclusion: N-substituted triazolo-azetidine scaffold may be used as a versatile starting point for the development of novel active and selective antibacterial compounds.

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