232 OIL SOURCES AND VITAMIN E IN THE DIET ON THE BOAR SEMEN QUALITY PRESERVED AT 5°C

2009 ◽  
Vol 21 (1) ◽  
pp. 214
Author(s):  
C. A. A. Torres ◽  
E. A. M. Amorim ◽  
L. S. Amorim ◽  
J. K. Graham ◽  
J. D. Guimarães ◽  
...  

Spermatozoa experience physical and chemical stresses during cooling and freezing as a result of ice formation and osmotic changes in the medium. Sperm cryosurvival appears to depend on intrinsic properties of the sperm plasma membrane, such as biochemical composition, thermal behavior, osmotic resistance, and the physical stresses determined by the protocol. The objective of this study was to evaluate the addition of oil sources and dietary supplementation of the vitamin E on boar sperm characteristics cooled at 5°C. Twenty-four mature Dalboard 85 boars, of proven fertility and in routine semen production for artificial insemination, were randomly divided, in factorial arrangement 2 × 3, with 2 oil sources (35 g kg–1 of soybean or salmon oil were added to the basal diet as a supplement) and 3 levels of antioxidants (150, 300, and 450 vitamin E mg kg–1). The antioxidant used in this study was vitamin E provided as DL-α-tocopheryl acetate. At the start of the experiment, the age of the boars ranged from 1 to 2 years old. Sperm samples were collected after 0, 1a, 4a, 7a, and 10a weeks of feeding the experimental diets, and total motility, vigor, hypoosmotic swelling (HOST), sperm morphology, and the fatty acid composition of the sperm were determined. Ejaculates from each of 24 boars were diluted to 3 × 109 sperm to 80 mL in a Beltsville thawing solution diluent and cooled to 5°C in a bottle (3 bottles/boar were cooled). After 24, 48, and 72 h, sperm samples from 1 bottle/boar were analyzed. Treatment differences for sperm parameters were determined using ANOVA. Dietary supplementation with salmon oil reduced (P < 0.05) the proportion of ω6 fatty acid in fresh sperm, specifically of docosapentaenoic acid (22:5ω6), and it increased (P < 0.05) the proportion of docosahexanoic acid (22:6ω3). The motility and HOST of the animals sperm treated with salmon oil at 5°C was superior (P < 0.05) than animals treated with soybean oil, after 24, 48, and 72 h. Feeding salmon oil also increased (P < 0.05) sperm vigor after 24 and 48 h. The motility, vigor, and HOST of sperm at 5°C differed (P < 0.05) over the time they were preserved, with values at 24 h of storage being superior to sperm stored for 48 h and both being superior to sperm stored for 72 h. The percentages of sperm with abnormal morphology increased (P < 0.05) in the semen from animals treated with soybean oil. The percentages of sperm with abnormal morphology were higher (P < 0.05) in the semen at 5°C after 24, 48, and 72 h. Feeding boars salmon oil improved the sperm characteristics of boar semen cooled at 5°C during the 24 and 48 h of preservation time without using a specific diluent designed for this temperature. Supported by grants from: CNPq, FAPEMIG, Perdigao S/A., MINITUB, and Lagoa da Serra.

2018 ◽  
Vol 58 (10) ◽  
pp. 1829
Author(s):  
M. Mohiti-Asli ◽  
M. Ghanaatparast-Rashti

This study investigated the effect of feeding vitamin E, vitamin C, and two sources of vegetable oil on immune response and meat quality of broilers. A total of 320 one-day-old chicks were used in a completely randomised design with eight treatments arranged as a 2 × 2 × 2 factorial with two levels of vitamin E (0 and 200 mg/kg), two levels of vitamin C (0 and 1000 mg/kg), and two sources of vegetable oil (soybean and canola). Dietary supplementation of either vitamin E or C increased (P < 0.05) secondary humoral response, whereas oil sources had no significant effect. Broilers fed soybean oil had lower cellular response to the phytohemagglutinin skin test than those fed canola oil in diet, and supplementation of vitamin E increased cellular immune response. However, fat, cholesterol and pH of meat were not affected by source of oil or antioxidants, lipid oxidation was higher (P < 0.05) in thigh and breast meat of broilers fed soybean oil than canola oil. Dietary supplementation of vitamin E decreased (P < 0.05) lipid oxidation in thigh and breast of broilers fed diet containing soybean oil, without any effect on meat oxidation of those fed canola oil. Dietary supplementation of vitamin C increased lipid oxidation in thigh meat of broilers (P < 0.05). It can be concluded that inclusion of soybean oil to the diet, compared with canola oil, increased need for antioxidant. Vitamin E had beneficial effects on immune response and reduced meat lipid oxidation; nonetheless future studies should explore the antioxidant effect of vitamin C in stored meat.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 202-203
Author(s):  
Ding Wang ◽  
Young Dal Jang ◽  
G K Rentfrow ◽  
H J Monegue ◽  
M J Azain ◽  
...  

Abstract The study objective was to assess the contribution of fat source and vitamin E (VE) supplementation on growth performance, antioxidant status, and fatty acid profile in the liver of pigs at heavy slaughter weight (148.09 ±1.64 kg). A total of 72 individually-fed pigs (36 barrows, 36 gilts; 28.55 ±1.16 kg) were randomly assigned to 12 dietary treatments in a 2 × 6 factorial arrangement. Fat treatments were tallow and corn-oil. The VE treatments included four levels of α-tocopheryl-acetate (ATA; 11, 40, 100, and 200 ppm) and two levels of mixed tocopherols (primarily γ-tocopherol; 40 and 100 ppm). Liver samples were collected at slaughter. Data analysis were performed by ANOVA using GLM in SAS. No effect of dietary fat sources, isoforms of VE, and levels of γ-tocopherol were detected on growth performance or liver antioxidant status (GSH, GSSG, SOD, and MDA). Increasing dietary ATA linearly increased overall ADG (0.98, 1.02, 1.02, and 1.08 kg/day; P = 0.02) from 28 to 150 kg; overall ADG of pigs fed γ-tocopherol-diets was 1.07 and 1.04 kg/day, respectively. An interaction between fat sources and ATA was observed on cumulative ADG during Phase1-3 (28-100 kg; P = 0.04) and Phase1-4 (28-125 kg; P = 0.03) wherein pigs fed corn-oil-diet, but not tallow-diet, had increased ADG with increased dietary ATA. Increasing dietary ATA quadratically increased SOD activity (P < 0.05; highest at 100 ppm), and quadratically decreased MDA content (P < 0.05; lowest at 40 ppm) in the liver. Liver fatty acid profile was affected by fat source (P < 0.05) but not VE treatment, pigs fed corn-oil-diets had less SFA (34.23 vs. 35.45%; P = 0.02) and MUFA (11.98 vs. 18.38%; P < 0.0001), but more PUFA (49.14 vs. 40.78%; P < 0.0001) than pigs fed tallow-diets in the liver. Beneficial effects of dietary VE supplementation were observed on growth performance and antioxidant status of pigs under conditions of this study.


Animals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 508 ◽  
Author(s):  
Shenfei Long ◽  
Sujie Liu ◽  
Di Wu ◽  
Shad Mahfuz ◽  
Xiangshu Piao

This study aimed to investigate the efficiency of dietary fatty acids from various sources on growth performance, meat quality, muscle fatty acid deposition and antioxidant capacity in broilers. 126 Arbor Acres broilers (1 d-old, initial body weight of 45.5 ± 0.72 g) were randomly assigned to three treatments with seven cages per treatment and six broilers per cage. The dietary treatments included: (1) corn–soybean meal basal diet containing 3% soybean oil (control diet, CTL); (2) basal diet + 1% microalgae + 1% linseed oil + 1% soybean oil (ML); (3) basal diet + 2% fish oil + 1% soybean oil (FS). The trial consisted of phase 1 (day 1 to 21) and 2 (day 22 to 42). Compared with CTL, broilers fed ML or FS diet showed improved (p < 0.05) average daily gain in phase 1, 2, and overall (day 1 to 42), as well as a decreased (p < 0.05) feed conversion ratio in phase 1 and overall. On day 42, broilers supplemented with FS diet showed increased (p ≤ 0.05) the relative weights of pancreas and liver, as well as higher (p < 0.05) redness value in breast and thigh muscle compared with CTL. Broilers offered ML or FS diet had lower (p < 0.05) the relative weight of abdominal fat and total serum cholesterol content in phase 1, and increased (p < 0.05) contents of serum glucose, n-3 polyunsaturated fatty acids (PUFA), eicosacagetaenoic acid, docosahexaenoic acid, glutathione peroxidase, superoxide dismutase and total antioxidant capacity, as well as lower (p < 0.05) concentrations of malondialdehyde, n-6 PUFA, and n-6/n-3 PUFA ratio in breast and thigh muscle compared with CTL. This research indicates that diets supplemented with fish oil or a combination of microalgae and linseed oil experience improved performance, antioxidant capacities and n-3 PUFA profile in muscle of broilers compared with traditional soybean oil supplemented diets


Reproduction ◽  
2001 ◽  
pp. 315-322 ◽  
Author(s):  
JA Rooke ◽  
CC Shao ◽  
BK Speake

The aim of the present study was to characterize the effects of feeding tuna oil on the lipid and fatty acid composition of boar spermatozoa and to relate changes in composition to boar semen characteristics. Ten boars were paired by age and allocated to one of two diets (five boars per diet). The diets, which were offered for 6 weeks, consisted of a basal diet that was either unsupplemented or supplemented with 30 g tuna oil kg(-1) diet. Adding tuna oil to the diet increased the ether extract concentration of the diets fed from 65 to 92 g kg(-1) dry matter and supplied 10.5 g long chain polyunsaturated (n-3) fatty acids per 100 g total fatty acids. There were no changes in semen fatty acid composition after 3 weeks of feeding tuna oil. However, after 5 and 6 weeks, the proportions (g per 100 g total fatty acids) of 22:6(n-3) in sperm phospholipid fatty acids were increased from 34.5 to 42.9 g by feeding tuna oil and 22:5(n-6) decreased from 29.8 to 17.9 g. No changes were observed in other sperm lipids or seminal plasma phospholipids as a result of the diets fed. Feeding tuna oil increased the proportion of spermatozoa with progressive motility and with a normal acrosome score and reduced the proportion of spermatozoa with abnormal morphologies.


2015 ◽  
Vol 55 (8) ◽  
pp. 1030 ◽  
Author(s):  
Fabiana Alves de Almeida ◽  
Américo Garcia da Silva Sobrinho ◽  
Gabriela Milani Manzi ◽  
Natália Ludmila Lins Lima ◽  
Viviane Endo ◽  
...  

This study aimed to evaluate the effects of supplementation with sunflower seeds and vitamin E for fattening lambs, on the chemical composition, cholesterol, vitamin E level, fatty acid profile and lipid oxidation of meat from sheep. Four treatments were evaluated, as follows: SC – basal diet consisting of sugarcane + concentrate; SCS – basal diet supplemented with sunflower seed; SCE – basal diet supplemented with 1000 mg vitamin E; SCSE – basal diet supplemented with sunflower seed and 1000 mg vitamin E. The Longissimus lumborum muscle of 32 Ile de France lambs, which were slaughtered when their bodyweight reached 32 kg, was used. The chemical composition of the lamb meat was not significantly different (P > 0.05) between the treatments. Sunflower seed supplementation increased (P < 0.05) the levels of linoleic acid, vaccenic acid and conjugated linoleic acid. Inclusion of vitamin E in the diet increased (P < 0.05) the concentration of vitamin E in the meat while decreasing lipid oxidation. Considering the current demand for healthy foods, inclusion of sunflower seeds and vitamin E in the diet of sheep is a viable possibility.


2000 ◽  
Vol 41 (sup001) ◽  
pp. 8-10 ◽  
Author(s):  
S. Cerolini ◽  
P. Surai ◽  
G. Mangiagalli ◽  
L.G. Cavalchini ◽  
R.C. Noble
Keyword(s):  

2008 ◽  
Vol 88 (3) ◽  
pp. 475-483 ◽  
Author(s):  
Maziar Mohiti-Asli ◽  
Farid Shariatmadari ◽  
Houshang Lotfollahian ◽  
Mohamad Taghi Mazuji

A 7-wk trial was carried out to investigate the effect of vitamin E and inorganic and organic selenium added to hens' diet on quality and lipid stability of eggs during storage. One hundred forty-four Hy-Line W-36 hens (63-wk of age) were divided into six equal groups. Five groups received a basal diet supplemented with 0.4 mg kg-1 sodium selenite or selenium yeast, 200 mg kg-1 vitamin E or a combination of selenium and vitamin E; whereas the control group received no supplementation. Hen production was assessed daily and fresh egg quality parameters were determined every 2 wk. Eggs were stored under different conditions (4°C, 23–27°C or 31°C) for 14 d. Eggs were analyzed for quality characteristics, egg component weight, Malondialdehyde values as a secondary oxidation product and yolk fatty acid (FA) composition. The performance of the hens and egg weights were not affected either by the source of the selenium or by the vitamin E. The inclusion of selenium or vitamin E in the diet significantly increased their concentrations in the egg. The supplemented diets also improved egg quality, oxidative stability and fatty acid composition during storage. Key words: Vitamin E, selenium, lipid peroxidation, egg fatty acid composition, storage


2020 ◽  
Vol 3 (10) ◽  
pp. 137-143
Author(s):  
Alagbe, J.O ◽  
Akintayo - Balogun Omolere. M

An experiment was carried out to determine the effects of dietary supplementation of Albizia lebbeck seed oil (ALO) on the fatty acid composition of weaner rabbits.50 weaned rabbits of mixed breed and sexes, aged between 6-7 weeks with an average initial body weight of 460 ± 1.3 were randomly assigned into five dietary treatments of ten rabbits per group; each group was further divided into 5 replicates consisting of two rabbits each. Basal diet was formulated to meet the nutritional requirements of rabbits according to NRC (1977). Rabbits in treatment 1, 2, 3, 4 and 5 were supplemented with ALO at 0 %, 0.1%, 0.2 %, 0.3 % and 0.4 % respectively. Feed and water was given ad libitum and the experiment lasted for 12 weeks.The results showed that significant differences (P<0.05) were observed in saturated fatty acid (SFA), polyunsaturated fatty acid (PUFA) and omega-6/omega -3 ratio (n-6:n-3) values obtained. Rabbits fed diet containing 0.4 % ALO had the highest PUFA value (54.17 %), followed by T4 (53.01 %), T3 (45.13 %), T2 (37.61 %) and T1 (26.93 %) respectively. Similarly (n-6:n-3) composition in T5 (3.65 %) increased in T5 fed 0.4 % ALO compared with T1 (1.38 %) fed 0% ALO.Antherogenic index were significantly (P<0.05) different among the treatments. It can be concluded that supplementation of ALO at 0.4 % highly influenced the composition of fatty acid in rabbit meat.


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