151 SEASONAL REPRODUCTION IN WILD AND CAPTIVE MALE KOALA POPULATIONS IN SOUTH-EAST QUEENSLAND

2009 ◽  
Vol 21 (1) ◽  
pp. 174
Author(s):  
C. D. Allen ◽  
D. L. de Villiers ◽  
B. D. Manning ◽  
D. S. Dique ◽  
M. Burridge ◽  
...  
Keyword(s):  
Seasonal Change ◽  
Plasma Membranes ◽  
Semen Quality ◽  
Animal Husbandry ◽  
Captive Population ◽  
Population Total ◽  
Free Range ◽  
Male Reproductive Function ◽  
Captive Populations ◽  
Service Staff

Seasonal changes in male reproductive function were assessed in a wild free-range population (n = 10; obtained every six weeks from January to November 2005), a deceased wild free-range population (n = 84; obtained monthly from September to August 2005) and a captive population (n = 7; obtained monthly from October 2005 to October 2006) of koalas in south-east Queensland. This study also determined the practicality of using free-range wild male koalas as potential semen donors for genome resource banks. Examination of a range of reproductive variables initially revealed no significant seasonal change in the 3 koala populations; however, when the data were adjusted to account for individual koalas, their size and/or their health status, the majority of reproductive parameters showed evidence of seasonal variation that was supported by statistical modeling. Relationships between variables were based on simple polynomials, up to a cubic for some variables. Total testicular volume changed throughout the year in the wild and captive populations with an increase over spring and summer and a decrease in autumn and winter; no such change was detected in the deceased population. Maximum area of the sternal gland stain occurred in spring in both the deceased and captive populations but in winter for the wild free-range population. Total bulbo-urethral gland volume in the deceased population showed an increase over spring, a decrease over summer and autumn and then an increase towards the end of winter. The steroidogenic capacity of the koala testis (testosterone secretion) in both the wild free-range (live) and captive populations showed a peak during spring and a nadir in autumn. The quality of semen samples collected by electroejaculation from the wild (live) and captive koala populations showed evidence of being influenced by season. Initial percentage motility of the wild population decreased marginally throughout the study and initial rate of sperm movement was highest in winter. Motility of spermatozoa after thawing from the wild koala population was also highest in winter as was the percentage of cryopreserved spermatozoa with intact plasma membranes collected from the captive population. This study has shown that male koala reproduction in south-east Queensland is seasonal and that it is possible to repeatedly collect semen from free-range koalas as potential genetic donors. Nevertheless, semen quality from captive and wild caught animals appears to be susceptible to seasonal change and winter appears to be the optimal season in which to collect such samples. We are grateful to the veterinary staff, zookeepers, and volunteers at Dreamworld for their assistance with general animal husbandry. We also sincerely thank the many veterinarians, volunteers, and Queensland Parks and Wildlife Service staff that assisted with this project whose help was invaluable for the accomplishment of this project. This project was funded by an Australian Research Council (ARC) Grant, the School of Animal Studies (SAS) The University of Queensland, and the Queensland Government’s Koala Enhanced Genetic Exchange Program (KEGEP).

Kualitas Semen Cair Kambing Boer Berbahan Pengencer Air Kelapa Muda Varietas Viridis Setelah Simpan Dingin

2019 ◽  
Vol 6 (1) ◽  
pp. 78
Author(s):  
Muhammad Ade Salim ◽  
Muhammad Nur Ihsan ◽  
Nur Isnaini ◽  
Trinil Susilawati
Keyword(s):  
Semen Quality ◽  
Block Design ◽  
Animal Husbandry ◽  
Egg Yolk ◽  
Coconut Water ◽  
Boer Goat ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Artificial Vagina

ABSTRAKAir kelapa muda varietas viridisdapat dijadikan pengencer aletrnatif semen cair bagi program IB di daerah minim sarana semen beku. Tujuan penelitian ini untuk menguji pengaruh penggunaan air kelapa muda viridissebagai bahan pengencer terhadap kualitas semen cair kambing Boer setelah didinginkan. Dilaksanakanselama 3 bulan di Laboratorium Fakultas Peternakan UBUnit SumberSekar,Malang. Metodenya yaitu eksperimen. Semen dari  3 pejantan Boer umur 3-5 tahun, dikoleksi seminggu sekali dengan VB. Air kelapa mudaviridis umur 5-7 bulan serta tris aminomethane sebagai kontrol. Didesain menggunakan Rancangan Acak Kelompok (RAK) dengan 2 perlakuan yaitu P0 (tris aminomethane + 10% KT) dan  P1 (air kelapa muda viridis + 10% KT) masing-masing diulang 10 kali. Data dianalisis dengan analisis Ragam (Anova) dengan software Genstat 18. Variabelnya yaitu motilitas individu, viabilitas dan abnormalitas. Hasil penelitian yaitu motilitas individu pada P1bertahan sampai 4 hari (40,5± 24,3%), viabilitas terbaik sampai hari ke-5 (42±24,6%), abnormalitas terendah di hari ke-7(1,31± 0,6). Kesimpulannya, Pengencer air kelapa muda viridis dapat mempertahankan kualitas semen cair kambing Boer selama 4 hari untuk motilitas dan 5 hari untuk viabilitas.Kata Kunci:pengencer, air kelapa, varietas viridisABSTRACTYoung viridis coconut water could be used as an alternative to liquid semen diluent for artificial insemination program in the area with limited facility for frozen semen production. This study evaluated the use of young coconut water as a diluent on liquid semen quality of Boer goat after cold storage. This study was carried out for 3 months at Sumber Sekar Laboratory, Faculty of Animal Husbandry, University of Brawijaya, Malang. The semen was collected from 3 Boer bucks aged at 3 to 5 years old. The semen collection was done once a week with the aid of artificial vagina. The diluents used were young Viridis coconut (5 to 7 months old) and tris aminomethane. The method used was an experiment in a randomized block design with 2 treatments and 10 replicates. The treatments used were T0: tris aminomethane + 10% egg yolk (control) and T1:  young Viridis coconut water + 10% egg yolk. Data were analyzed by analysis of variance using Genstat 18 software. The variables measured were sperm individual motility, viability, and abnormality. The results showed that the sperm individual motility in T1 survived up to 4 days (40.5± 24.3%), the best viability at 5 days (42.0±24.6%),  while the lowest abnormality at 7 days (1.31±0.6). It could be concluded that: 1. Tris aminomethane diluent has higher quality with the storage length up to 9 days, 2. Young Viridis coconut water diluent could preserve liquid semen quality of Boer goat up to 4 days for sperm motility and 5 days for sperm viability.Keywords: diluents, coconut water, viridis variety

The Prevalence of Bacteriospermia in Infertile Men and Association with Semen Quality in Southwestern Iran

2020 ◽  
Vol 20 (2) ◽  
pp. 198-202 ◽  
Author(s):  
Mohammad Motamedifar ◽  
Yalda Malekzadegan ◽  
Parisa Namdari ◽  
Behzad Dehghani ◽  
Bahia Namavar Jahromi ◽  
...  
Keyword(s):  
Semen Quality ◽  
Sperm Quality ◽  
Reproductive Function ◽  
World Health ◽  
Public Health Issue ◽  
Control Group ◽  
Microbiological Analysis ◽  
Male Reproductive Function ◽  
Infertile Men ◽  
Southwestern Iran

Introduction: Infertility considered as a social and public health issue and estimated that most of these infertile couples are residents of developing countries. Infectious diseases including the history of Sexually Transmitted Infections (STIs) may impact on male reproductive function. Therefore, the present study aimed to investigate the prevalence of bacterial contaminants of semen and probable association with sperm quality of infertile men in Iranian population. Methods: The study population consisted of 200 infertile men and 150 fertile men attending an infertility Center in southwestern Iran during the study period in 2015. The assessment of sperm parameters was according to the World Health Organization (WHO) guidelines. The presumptive pathogens were identified using standard microbiology tests and confirmed by specific PCR primers. Results: The prevalence of bacteriospermia in the semen of the infertile group was significantly higher than that in the fertile group (48% vs. 26.7%, P <0.001). The microbiological analysis of samples showed that the most abundant species of bacteria in semen of infertile men were Chlamydia trachomatis (12.5%) followed by Neisseria gonorrhoeae (11%). On the other hand, in the control group, Lactobacillus spp. (17.3%) was the most isolated pathogen. Results showed that the presence of N. gonorrhoeae, C. trachomatis, Mycoplasma genitalium, Haemophilus, and Klebsiella was significantly associated with sperm abnormality. Conclusion: Based on our findings, it seems that bacteriospermia is associated with alterations in the properties of semen which may lead to a decrease in the fertilization potential of sperm. Therefore, immediate and appropriate treatment is necessary before investigating every other possible cause of infertility.

scholarly journals Comparison of Serum Inhibin B and Follicle-Stimulating Hormone (FSH) Level between Normal and Infertile Men in Yaoundé

2020 ◽  
Vol 2020 ◽  
pp. 1-9 ◽  
Author(s):  
Tchoula Mamiafo Corinne ◽  
Pieme Constant Anatole ◽  
Ngogang Yonkeu Jeanne
Keyword(s):  
Semen Quality ◽  
Follicle Stimulating Hormone ◽  
Sperm Concentration ◽  
Reproductive Hormones ◽  
Inhibin B ◽  
World Health ◽  
Infertile Couple ◽  
Male Reproductive Function ◽  
Significant Difference ◽  
Stimulating Hormone

Objective. Hormones play a vital role in initiating and maintaining male reproductive function. The present study explores the influence and predictive ability of two reproductive hormones on semen quality among men who were partners in an infertile couple. Design. During our cross sectional study, men were recruited from private and public hospital and laboratories for clinical evaluation of fertility status. Methods. Fresh semen samples were assessed for quality (concentration, motility and morphology) according to the 2010 World Health Organization manual and the serum levels of hormones, including follicle-stimulating hormone (FSH), Inhibin B was measured (ELISA). Results. We found a significant difference in the two groups regarding sperm concentration (p<0.0001), total sperm count (p<0.0001), progressive motility (p<0.0001), vitality (p<0.0001) and the percentage of normal forms (p=0.043). We found a strong and negative correlation between FSH and Inhibin B in the overall population, the normozoospermic reference group and the case group. Our study confirmed that Inhibin B was significantly and positively correlated with sperm concentration and leucocytes, and that FSH was negatively correlated to sperm concentration and vitality. Conclusion. Consistent with other studies, our results show strong association between semen quality and FSH and Inhibin B.

scholarly journals Coenzyme Q10, oxidative stress, and male infertility: A review

2021 ◽  
Vol 48 (2) ◽  
pp. 97-104
Author(s):  
Ahmed T. Alahmar ◽  
Aldo E. Calogero ◽  
Rajender Singh ◽  
Rossella Cannarella ◽  
Pallav Sengupta ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Male Infertility ◽  
Coenzyme Q10 ◽  
Semen Quality ◽  
Sperm Count ◽  
Specific Treatment ◽  
Physiological Role ◽  
Semen Parameters ◽  
Sperm Dna Fragmentation ◽  
Male Reproductive Function

Male infertility has a complex etiopathology, which mostly remains elusive. Although research has claimed that oxidative stress (OS) is the most likely underlying mechanism of idiopathic male infertility, the specific treatment of OS-mediated male infertility requires further investigation. Coenzyme Q10 (CoQ10), a vitamin-like substance, has been found in measurable levels in human semen. It exhibits essential metabolic and antioxidant functions, as well as playing a vital role in mitochondrial bioenergetics. Thus, CoQ10 may be a key player in the maintenance of biological redox balance. CoQ10 concentrations in seminal plasma directly correlate with semen parameters, especially sperm count and sperm motility. Seminal CoQ10 concentrations have been shown to be altered in various male infertility states, such as varicocele, asthenozoospermia, and medical or surgical regimens used to treat male infertility. These observations imply that CoQ10 plays an important physiological role in the maintenance and amelioration of semen quality. The present article thereby aimed to review the possible mechanisms through which CoQ10 plays a role in the regulation of male reproductive function, and to concisely discuss its efficacy as an ameliorative agent in restoring semen parameters in male infertility, as well as its impact on OS markers, sperm DNA fragmentation, pregnancy, and assisted reproductive technology outcomes.

scholarly journals Molecular Characterization Reveals Genetic Differences Between Wild and Captive Populations of Mandiangin Giant Gourami (Osphronemus goramy)

2019 ◽  
Vol 4 (1) ◽  
pp. 1-10
Author(s):  
Agus Nuryanto ◽  
Maria Bramasti Susilo ◽  
Adi Amurwanto
Keyword(s):  
Wild Population ◽  
Breeding Strategy ◽  
Genetic Differences ◽  
Genetic Alteration ◽  
Rflp Markers ◽  
Captive Population ◽  
Freshwater Aquaculture ◽  
Captive Populations ◽  
Pcr Rflp ◽  
Scientific Base

  Captive population of Mandiangin giant gourami (Osphronemus goramy Lac.) is a new strain developed from wild pop-ulations in Riam Kanan Dam. No much is known about the genetic constituent of this strain compared to the parental population. This re-search was done to obtain information whether genetic alteration has occurred in captive population compared to their wild parental pop-ulation. Wild population was caught from Riam Kanan Dam, while captive population was collected from Balai Perikanan Budidaya Air Tawar (BPBAT) “Freshwater Aquaculture Centre” Mandiangin, South Kalimantan. The captive population of Batanghari strain from BPBAT Sungai Gelam, Jambi was also analyzed. Molecular assess-ment was done using CO1 PCR-RFLP which was developed by digest-ing the amplicon of the CO1 gene from all populations using two re-striction enzymes, namely HindIII and TaqI and resulted CO1-HindIII and CO1-TaqI RFLP markers. All tested populations showed uniform patterns of those CO1-RFLP markers but with different fragment sizes among populations. This means all resulted RFLP markers were mono-morphic in each populations but polymorphic among giant gourami populations. In other words, no genetic diversity within population but substantial genetic differences was observed between wild and cap-tive Mandiangin populations. This proved that genetic alteration has occurred in Mandiangin captive populations compared to the wild pa-rental giant gourami population. In conclusion, captive and wild pop-ulations of Mandiangin giant gourami were genetically different. This result is vital as scientific base for future development of inland fish-eries, especially for breeding strategy of Mandiangin giant gourami. 

ANALISIS PENGEMBANGAN WILAYAH PETERNAKAN SAPI POTONG DI KABUPATEN SUBANG

2020 ◽  
Vol 1 (2) ◽  
Author(s):  
Ferdi Fathurohman
Keyword(s):  
Beef Cattle ◽  
Animal Health ◽  
Secondary Data ◽  
Animal Husbandry ◽  
Primary Data ◽  
Cattle Population ◽  
Location Quotient ◽  
Potential Region ◽  
Population Total ◽  
Independent Variable

This research aims to analyze the development potential of the regions of beef cattle and the factors that influence the development of beef cattle in the Regency of Subang. Research carried out by analyzing the primary data from a breeder and secondary data are sourced from the Department of animal husbandry and animal health District of Subang, food security and animal husbandry Office of West Java province and the Central Bureau of statistics the Regency of Subang. The data are analyzed in a descriptive and statistical. Analysis of the potential of the region using the parameters of location quotient (LQ) and factors development of beef cattle were analyzed by linear regression model, with multiple dependent factor (Y) beef cattle Population and the independent variable (X) from the a row is beef cattle population total population of Subang, County, and sales of beef cattle out of the Kabupaten Subang. Keywords: Potential, Region, Beef Cattle

Determination of the presence of Mycobacterium avium on Guam as a precursor to reintroduction of indigenous bird species

1998 ◽  
Vol 4 (3) ◽  
pp. 227
Author(s):  
Ilse Silva-Krott ◽  
M. Kelly Brock ◽  
Randall E. Junge
Keyword(s):  
Mycobacterium Avium ◽  
Captive Breeding ◽  
Bird Species ◽  
Animal Husbandry ◽  
Breeding Population ◽  
Native Forest ◽  
Captive Population ◽  
Boiga Irregularis ◽  
Bird Populations ◽  
High Degree

Eight of 11 native forest bird species on Guam were extirpated by the introduction of the Brown Tree Snake Boiga irregularis. Emergency measures necessary to rescue the Guam subspecies of Micronesian Kingfisher Halcyon cinnamomina cinnamomina from extinction involved translocation and captive breeding in American mainland zoos. Soon after the establishment of a captive breeding population, the kingfisher demonstrated a high degree of susceptibility to avian tuberculosis (ATB), a disease that proved to be a major threat to the preservation of the species. The cause of ATB is Mycobacterium avium which produces a prolonged course of infection in kingfishers and other birds. Kingfishers infected with M. avium are difficult to detect until late in the course of the disease, thereby potentially posing a risk of transmitting ATB to the Guam captive population of Guam Rails Gallirallus owstoni, if kingfishers are repatriated. M. avium is considered to be ubiquitous in nature. However, there are no reported mortalities due to ATB in any bird species on Guam. In this study, six of twenty-one cultures yielded Mycobacterium spp., two of which were further identified as M. avium. Since this study demonstrates that M. avium already exists on Guam, repatriating kingfishers to Guam poses no threat of introducing a new pathological agent to the island's ecosystem. Strict quarantine procedures along with rigorous animal husbandry protocols should minimize risks of repatriating infected kingfishers to Guam, and prevent transmission of ATB to the captive population of Guam Rails and other bird populations on Guam.

scholarly journals Association between GH receptor polymorphism (exon 3 deletion), serum IGF1, semen quality, and reproductive hormone levels in 838 healthy young men

2014 ◽  
Vol 170 (4) ◽  
pp. 555-563 ◽  
Author(s):  
M Andreassen ◽  
R B Jensen ◽  
N Jørgensen ◽  
A Juul
Keyword(s):  
Semen Quality ◽  
Final Height ◽  
Reproductive Function ◽  
Postnatal Growth ◽  
Reproductive Hormones ◽  
Inhibin B ◽  
Sex Hormone Binding Globulin ◽  
Gh Receptor ◽  
Male Reproductive Function ◽  
Semen Volume

IntroductionGH activity may be involved in male reproductive function. A common genetic polymorphism in the gene encoding the GH receptor (GHR) results in deletion of the entire exon 3 sequence (GHRd3 isoform). The short GHRd3/d3 isoform seems more sensitive compared with full-length receptors (GHRfl/fl).AimTo investigate the associations between GH activity, evaluated by exon 3 GHR polymorphism, and serum IGF1 vs reproductive hormones, semen quality, and pre- and postnatal growth in healthy young males (n=838, mean age: 19.4 years).ResultsCompared with GHRfl/fl homozygous individuals (n=467) GHRd3/d3 homozygous individuals (n=69) tended to have larger semen volume (3.2 (2.4–4.3) vs 3.6 (2.6–4.7) ml, P=0.053) and higher serum inhibin-B levels (208 pg/ml (158–257) vs 227 pg/ml (185–264), P=0.050). Semen quality, levels of gonadotropins, testosterone, estradiol, sex hormone-binding globulin, and IGF1 were not associated with GHRd3 genotype.A twofold increase in serum IGF1 was associated with a 13% (4–23) increase in calculated free testosterone (P=0.004). By contrast IGF1 was inversely associated with serum inhibin-B (P=0.027), but showed no associations to semen quality. GHR genotype and serum IGF1 were not associated with size at birth or final height.ConclusionsGHRd3 polymorphism seemed only to have a weak influence on male reproductive function of borderline significance. The sensitive GHRd3/d3 genotype may slightly increase testicular function, as evaluated by semen volume and levels of inhibin-B, but does not seem to influence Leydig cell steroidogenesis. GHR genotype did not influence pre- and postnatal growth.

scholarly journals Retriever and Pointer: Software to Evaluate Inbreeding and Genetic Management in Captive Populations

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1332
Author(s):  
Jack J. Windig ◽  
Ina Hulsegge
Keyword(s):  
Population Structure ◽  
Allelic Diversity ◽  
Captive Population ◽  
Pedigree Data ◽  
Effective Population ◽  
Genetic Management ◽  
Captive Populations ◽  
Population Structures ◽  
Population Sizes ◽  
Well Data

The Retriever and Pointer software has been developed for genetic management of (small) captive populations The Retriever program uses as input pedigree data and extracts data on population structure that determine inbreeding rates such as skewness of sire contributions. Levels and rates of inbreeding and kinship and effective population sizes are determined as well. Data on population structure can be used as input for the Pointer program. This program uses stochastic simulation to evaluate a population and provides expected levels and rates of inbreeding and kinship, and optionally allelic diversity. The user can simulate different options for genetic management such as sire restrictions, restrictions on inbreeding levels, mean kinships and breeding circles. Both Retriever and Pointer can analyze populations with subpopulations and different rates of exchange between them. Although originally devised for dogs, the software can be, and has been, used for any captive population including livestock and zoo populations, and a number of examples are provided. The pointer software is also suitable in education where students may generate their own populations and evaluate effects of different population structures and genetic management on genetic diversity. Input is provided via a graphical user interface. The software can be downloaded for free.

Levels of seminal tRNA-derived fragments from normozoospermic men correlate with the success rate of ART

2021 ◽  
Vol 27 (4) ◽  
Author(s):  
Julieta B Grosso ◽  
Luciana Zoff ◽  
Karina L Calvo ◽  
María Belén Maraval ◽  
Mariana Perez ◽  
...  
Keyword(s):  
Semen Quality ◽  
Physiological Stress ◽  
Sperm Quality ◽  
Semen Analysis ◽  
Reproductive Function ◽  
Medical Problem ◽  
Sperm Dna Fragmentation ◽  
Male Factor ◽  
Male Reproductive Function ◽  
A Prospective Study

Abstract Decreased fertility is becoming an important social and medical problem and the male factor is involved in at least half of infertility cases. Since conventional semen analysis provides limited prediction of male fertility; in this work, we evaluated the potential use of seminal small RNAs (sRNA) as markers of semen quality in ART. Our bioinformatic analyses of available sRNA-seq databases showed that the most abundant sRNA species in seminal plasma of normozoospermic men are tRNA-derived fragments (tRFs), a novel class of regulatory sRNAs. These molecules not only exert their function within cells but also are released into the extracellular environment where they could carry out signaling functions. To evaluate whether the assessment of seminal tRFs in normozoospermic men has a predictive value for the clinical outcome in ART, we performed a prospective study with couples who underwent ICSI cycles with donated oocytes. The results obtained demonstrated that levels of 5’tRF-Glu-CTC, 5’tRF-Lys-CTT, and 5’tRF-Gly-GCC are significantly elevated in seminal samples from cases with repeated failed ICSI cycles, suggesting a potential association between increased seminal tRFs and unexplained male infertility. Interestingly, these tRFs showed a negative association with seminal testosterone, highlighting their involvement in male endocrinology. Our findings also suggest that tRFs could play a role in modulating male reproductive function in response to physiological stress since they showed significant associations with the levels of sperm DNA fragmentation in couples that achieved pregnancy but not in cases with failed ICSI cycles where seminal cortisol levels correlate with sperm quality.

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