scholarly journals 143 SUPEROVULATORY RESPONSE AND EMBRYO PRODUCTION INFLUENCED BY THE ADDITION OF LH AND EFFECT OF THE REPEATABILITY IN SANTA INES SHEEP

2009 ◽  
Vol 21 (1) ◽  
pp. 171
Author(s):  
M. E. F. Oliveira ◽  
I. C. C. Santos ◽  
J. S. P. Pieroni ◽  
R. M. Ferreira ◽  
M. F. Cordeiro ◽  
...  
Keyword(s):  
Buenos Aires ◽  
Animal Health ◽  
Ovulation Rate ◽  
Embryo Production ◽  
Chi Square ◽  
Pfizer Animal Health ◽  
Santa Inês ◽  
Intravaginal Device

The aim this study was to evaluate the effect of the addition of LH in superovulatory response and embryo production in Santa Inês sheep. Ten donors with 60.3 ± 10.7 kg and BCS of 3.9 ± 0.3 were superovulated in a cross-over design, with a 60-day interval. Estrus was synchronized with a progesterone-releasing intravaginal device (CIDR™; Pfizer Animal Health, Brazil) inserted on Day 0 and replaced by a new one on Day 7, that was maintained to Day 14. Two doses of 37.5 g of D-cloprostenol (Prolise™, Arsa, Buenos Aires, Argentina) were administered, on Days 7 and 14. Donors also receive 256 mg of pFSH (Folltropin™, Bioniche, Belleville, ON, Canada) in 8 decreasing doses, starting on Day 12. On Day 14, all females received 200 IU of eCG (Novormon ™, Syntex, Argentina). On Day 15, the animals were homogeneously allocated in 1 of 2 groups: Control (GC, n = 10) and treated (G-LH, n = 10). Ewes in GC did not receive exogenous LH, whereas ewes in G-LH were treated with 7.5 mg of LH (Lutropin™, Bioniche), on Day 15. All females were inseminated by laparoscopy, with frozen–thawed semen, 42 and 48 h after CIDR removal. On Day 21, the embryos were surgically collected. The superovulatory response was classified in scores: (0) 4 or fewer CL; (1) between 5 and 10 CL, and (2) 11 or more CL. Means were compared using Kruskal-Wallis test and percentages using chi-square (P < 0.05). Most of donors (70%, 7/10) from G-LH presented a superovulatory response classified as score 2, and the remaining (30%, 3/10) as score 1, whereas, half of the controls were classified as score 2 and half as score 1. Ovulation rate tended to be greater in G-LH (135/158, 85.4% v. 105/135, 77.7%, P = 0.08). The number of CL (mean ± SD) was 10.5 ± 3.8 in GC and 13.5 ± 4.84 in G-LH, but was not statistically different. The number of anovulatory follicles (AF) did not differ between groups (GC: 3.0 ± 3.2; G-LH: 2.3 ± 1.6), but the proportion of AF tended to decrease in G-LH (30/135, 22.2% v. 23/158, 14.5%, P = 0.08). Considering embryo production, there was no difference between GC and G-LH (P > 0.05) related to number of recovered ova/embryos (6.1 ± 4.6 v. 8.4 ± 5.2), viable embryos (3.8 ± 4.3 v. 4.2 ± 5.2), unfertilized (1.7 ± 3.4 v. 2.0 ± 2.9) and degenerated embryos (0.7 ± 0.7 v. 2.2 ± 2.9), respectively. Data showed that the addition of LH tended to increase ovulation rate and to decrease the proportion of AF, but did not affect the number of viable embryos.

122 TIMED ARTIFICIAL INSEMINATION IN WOOD BISON USING FROZEN-THAWED SEMEN

2016 ◽  
Vol 28 (2) ◽  
pp. 191 ◽  
Author(s):  
G. P. Adams ◽  
S. X. Yang ◽  
J. M. Palomino ◽  
M. Anzar
Keyword(s):  
Animal Health ◽  
Egg Yolk ◽  
Ovulation Rate ◽  
Chi Square ◽  
Semen Cryopreservation ◽  
Wood Bison ◽  
The Mean ◽  
Intravaginal Device ◽  
Synchronization Procedure

Recent progress with methods to control ovulation and semen cryopreservation in Wood Bison was the impetus to test the feasibility of timed AI to facilitate reclamation of this threatened species. A 2 × 2 design was used to compare the efficacy of 2 ovulation synchronization techniques and 2 semen cryopreservation protocols. Female Wood Bison were assigned randomly to 2 groups (n = 24/group) in which ovarian synchronization was induced by ultrasound-guided ablation of follicles >5 mm or intramuscular treatment with 2.5 mg of estradiol 17B + 50 mg of progesterone (E+P) in canola oil. A progesterone-releasing intravaginal device (PRID) was placed at the time of follicle ablation (for 5 days) or E+P treatment (for 8 days) in the respective groups. A luteolytic dose of prostaglandin was given at the time of PRID removal, and 2500 IU of hCG was given IM 3 days later. Bison were inseminated 24 and 36 h after hCG treatment using frozen-thawed semen. The semen was collected by electro-ejaculation from 4 Wood Bison bulls, pooled, and divided into aliquots diluted in either egg-yolk extender (EY) or cholesterol-loaded cyclodextrin extender (CLC). Half the bison in each synchronization group were inseminated with either EY- or CLC-extended semen. Bison were examined by ultrasonography every 12 h beginning on the day of hCG treatment for 3 days or until ovulation was detected, whichever occurred first. Pregnancy diagnosis was made by ultrasonography 34–36 days after insemination. Two bison were excluded during the experiment because of handling difficulty; therefore, the total number of bison used was 46. Ovulation rate and interval to ovulation were compared between synchronization groups by chi-square and t-test, respectively. Pregnancy rates were compared among groups by 2-way ANOVA after transforming data to arcsin. The ovulation rate was not different between synchronization groups [combined mean, 37/46 (80%)], nor was the degree of synchrony, as assessed by the residuals (variation from the mean) in the respective groups. However, the diameter (mean ± standard error of the mean) of the dominant follicle at the time of hCG treatment was smaller in the follicle ablation group than in the E+P group (10.5 ± 0.6 v. 13.9 ± 0.6; P < 0.04), and the interval from hCG treatment to ovulation tended to be longer (35.3 ± 1.6 v. 31.8 ± 1.3 h; P ≤ 0.10). Pregnancy rate was not affected by synchronization procedure, but pregnancy was detected only in the EY-inseminated group (9/23 v. 0/23; P < 0.01). Despite that post-thaw sperm motility was similar for EY and CLC semen (41.7 ± 2.9 and 44.6 ± 3.3%; respectively), CLC-treated semen failed to impregnate bison in vivo. We concluded that synchronization and timed insemination with frozen-thawed semen is feasible in Wood Bison. Of the 23 bison inseminated with EY-extended semen, 21 ovulated (91%), and of those that ovulated 9 became pregnant (43%). Both synchronization schemes were effective, but the ablation protocol may be improved by an additional day between ablation and hCG treatment. We thank Vetoquinol Canada and Merck Animal Health for providing hormone treatments.

231 SUPEROVULATORY RESPONSE AND EMBRYO YIELD OF SANTA INES EWES SUBMITTED TO FOLLICLE STIMULATING HORMONE TREATMENTS STARTED NEAR THE TIME OF EMERGENCE OF THE FIRST AND LAST FOLLICULAR WAVE OF A PROGESTERONE-BASED PROTOCOL

2012 ◽  
Vol 24 (1) ◽  
pp. 228
Author(s):  
M. E. F. Oliveira ◽  
C. C. D'Amato ◽  
L. G. Oliveira ◽  
S. D. Bicudo ◽  
J. F. Fonseca ◽  
...  
Keyword(s):  
Buenos Aires ◽  
Animal Health ◽  
Follicle Stimulating Hormone ◽  
Corpora Lutea ◽  
Chi Square ◽  
Follicular Waves ◽  
Follicular Wave ◽  
Embryo Yield ◽  
Santa Inês ◽  
Follicular Dynamics

This study was designed to investigate if the superovulatory response and embryo yield in Santa Ines ewes are influenced by the time that FSH treatments are inititiated: near the emergence of the first or last follicular wave of a progesterone-based (P4) protocol. Days of emergence of the follicular waves was defined in a previous study that evaluated follicular dynamics in oestrus synchronization treatments (Oliveira et al. 2011 Acta Sci. Vet. 40). Twenty Santa Ines cyclic ewes were submitted to 2 superovulatory protocols according to the time that FSH treatments were initiated (G-first wave, n = 10 and G-last wave, n = 10). Thus, the FSH treatment started on Day 4 and 10 of protocol for G-first and G-last, respectively (Day 0 = onset of protocol). Ewes were treated with a P4 device (CIDR®; Pfizer-New Zealand) for 6 and 12 days for G-first and G-last, respectively. Additionally, in G-last, the CIDR was replaced by a new one on Day 7. All ewes received 2 injections of 37.5 μg of D-cloprostenol (Prolise®, Arsa-Argentina) on Day 0 and at CIDR removal. The superovulatory regimen consisted of 8 IM injections of pFSH (Folltropin®; Bioniche Animal Health, Belleville, Ontario, Canada) administrated twice daily (40, 40, 30, 30, 20, 20, 10 and 10 mg of porcine FSH). A single IM dose of 200 IU of eCG (Novormon®, Syntex, Buenos Aires, Argentina) was given concurrently with CIDR removal. Ewes were mated by a fertile ram. Embryo collections were accomplished 7 days after CIDR removal. The superovulatory response was classified in scores: (0) 4 or fewer corpora lutea (CL); (1) between 5 and 10 CL and (2) 11 or more CL. Means (± SD) were compared using Kruskal-Wallis test and percentages using Chi-square (P < 0.05). Most donors (70%, 7/10) from G-last had a superovulatory response classified as score 2 and the remainder (30%, 3/10) as score 1, whereas half of ewes from G-first were classified as score 2 and half as score 1 (P > 0.05).There was no effect between treatments (P > 0.05) for ovulation rate (G-first: 90.7 ± 10.2% vs G-last: 93.0 ± 7.2%), number of ovulations (G-first: 13.1 ± 7.3 vs G-last: 14.0 ± 5.8), or number of luteinized unovulated follicles (G-first: 1.4 ± 1.3 vs G-last: 0.9 ± 1.0). Furthermore, there was no effect between G-first and G-last (P > 0.05) in the rate of ova/embryos recovered (55.1 ± 27.1% vs 67.0 ± 19.4%), mean number of ova/embryos recovered (7.0 ± 5.5 vs 9.1 ± 3.8), number of viable embryos (3.9 ± 6.1 vs 5.7 ± 4.4), or viability rate (49.1 ± 43.7 vs 58.5 ± 34.6). In conclusion, superovulatory response and embryo yield did not differ between FSH treatments initiated during the first or last follicular wave of a progesterone-based treatment in Santa Ines ewes. Financial support of FAPESP is acknowledged.

387 EFFECT OF NUMBER OF INSEMINATIONS ON EMBRYO PRODUCTION IN SUPERSTIMULATED NELORE (BOS INDICUS) COWS SUBJECTED TO FIXED-TIME ARTIFICIAL INSEMINATION

2007 ◽  
Vol 19 (1) ◽  
pp. 309
Author(s):  
C. M. Martins ◽  
H. Ayres ◽  
J. R. S. Torres-Junior ◽  
A. H. Souza ◽  
G. A. Bó ◽  
...  
Keyword(s):  
Animal Health ◽  
Bos Indicus ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Ovulation Rate ◽  
Embryo Production ◽  
Chi Square ◽  
Chi Square Test ◽  
Level Of Significance ◽  
Ovulatory Follicles

In embryo transfer programs, 2 inseminations per superstimulated donor are usually recommended. Based on studies of follicular dynamics in Bos indicus donors (Baruselli et al. 2006 Theriogenology 65, 77–88), we tested the hypothesis that a single fixed-time AI (FTAI) at 16 h after pituitary luteinizing hormone (pLH) treatment is as efficacious as 2 FTAI at 12 and 24 h after the pLH treatment. Suckling Nelore cows (n = 10), 60 � 12 days postpartum, were equally distributed in 2 experimental groups: G-1TAI vs. G-2TAI, in a crossover design. All animals received an intravaginal progesterone device (DIB; Syntex S.A., Buenos Aires, Argentina) and 2 mg of estradiol benzoate (IM, Ric Be; Syntex) at random stages of the estrous cycle (Day 0). Superstimulatory treatments started on Day 4 with pFSH (133 mg; Folltropin-V; Bioniche Animal Health Canada, Inc., Belleville, Ontario, Canada) in 8 decreasing doses, given IM 12 h apart. On Day 6, all cows received 2 (AM and PM) treatments of 0.15 mg of d-cloprostenol (Prolise; Syntex). The DIB devices were removed 36 h after the first PGF2� injection. The pLH treatment (25 mg IM; Lutropin-V; Bioniche) was given 48 h after the first PGF2� treatment (12 h after the last pFSH treatment). In the G-2TAI group, all cows received 2 inseminations (12 and 24 h after the pLH). Cows in the G-1TAI group received a single insemination (16 h after the pLH). All inseminations were done with the same batch of semen from a single bull. Ultrasound examinations (PIE Medical Scanner 200; Pie Medical Equipment, Maastricht, The Netherlands) were performed every 12 h from 0 to 72 h after pLH treatment to evaluate the number of ovulatory follicles (&lt;8 mm), the ovulation rate, and the time of ovulation. Variables were analyzed by ANOVA and chi-square test. The homogeneity of the variances (distribution of ovulations) was analyzed by Bartlett&apos;s test. Conclusions were based on a 5% level of significance. The results for G-1TAI and G-2TAI were, respectively: number of follicles &gt;8 mm at the time of pLH administration (16.2 � 1.4 vs. 14.8 � 1.2; P &gt; 0.05), ovulation rate (63.8 � 3.8% vs. 64.2 � 4.3%; P &gt; 0.05), interval from the first to the last ovulation (32.4 � 1.8 vs. 33.6 � 1.6 h; P &gt; 0.05), total ova/embryos (8.2 � 0.9 vs. 7.2 � 0.8; P &gt; 0.05), Grade 1 embryos (2.0 � 0.5 vs. 2.3 � 0.4; P &gt; 0.05), transferable embryos (Grades 1, 2, and 3) (4.3 � 0.7 vs. 4.2 � 0.6; P &gt; 0.05), freezable embryos (Grades 1 and 2) (2.9 � 0.6 vs. 2.8 � 0.4; P &gt; 0.05), unfertilized ova (0.6 � 0.2 vs. 0.8 � 0.2; P &gt; 0.05), and degenerated embryos (3.3 � 0.9 vs. 2.2 � 0.3; P &gt; 0.05). These results suggest no significant differences in embryo production between superstimulated Nelore donors inseminated once or twice by fixed time following administration of pLH. This work was supported by Tecnopec, Brazil.

scholarly journals Effect of pFSH dose reduction on in vivo embryo production in Dorper ewes

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4215
Author(s):  
João Bosco Loiola Filho ◽  
Alane Pains Oliveira do Monte ◽  
Thais Thatiane Dos Santos Souza ◽  
Mayara De Souza Miranda ◽  
Lívia Correia Magalhães ◽  
...  
Keyword(s):  
Dose Reduction ◽  
Fertilization Rate ◽  
Corpora Lutea ◽  
Device Removal ◽  
Embryo Production ◽  
Chi Square ◽  
Estrus Detection ◽  
Chi Square Test ◽  
Intravaginal Device

To evaluate the effect of pFSH dose on the in vivo embryo production of Dorper ewes in the semi-arid northeast of Brazil, 40 sheep females were distributed into two groups of 20 animals that received intravaginal CIDR for 14 days, and two days before device removal, they received one of the following treatments: in the FSH200 group, the ewes received 200 mg of pFSH; and in the FSH128 group, the ewes received a total of 128 mg in decreasing doses every 12 h. Beginning 12 h after the conclusion of the treatments, estrus detection was performed every four hours using two Dorper rams of proven fertility. The ewes were mated at estrus onset and 24 hours later. Seven days after intravaginal device removal, the superovulatory response was evaluated, and embryo collection was performed using the laparotomy method. The recovered flushings were subjected to embryo searches under a stereomicroscope and classified according to their qualities. Analyses of variance (ANOVAs) and LSD tests were used to compare the different parameters. The data expressed as percentages were analysed by chi-square test. The ovulation rate was higher in the FSH200 group, which had 16.3 ± 0.3 corpora lutea (CL), than in the FSH128 group, which had 11.3 ± 0.3 CL (P<0.05). However, higher fertilization rate (83.6% vs. 62.4%) and higher transferable (86.0% vs. 71.6%) and freezable (67.9% vs. 40.8%) embryo rates were observed in the FSH 128 group compared with the group that received 200 mg. Furthermore, no significant differences in the remaining parameters were observed between the experimental groups (P>0.05), demonstrating that pFSH dose reduction promoted a greater production of freezable and transferable embryos in Dorper ewes subjected to MOET.

402 SUPEROVULATION OF TROPICAL-ADAPTED BOS TAURUS AND BOS INDICUS COWS IN A TROPICAL ENVIRONMENT

2010 ◽  
Vol 22 (1) ◽  
pp. 357
Author(s):  
R. H. Alvarez ◽  
A. C. Martinez ◽  
R. M. L. Pires
Keyword(s):  
Ovarian Function ◽  
Bos Taurus ◽  
Animal Health ◽  
Bos Indicus ◽  
Ovarian Response ◽  
Estradiol Benzoate ◽  
Maximum Temperature ◽  
Embryo Production ◽  
Chi Square ◽  
Average Maximum

Breed differences in ovarian function were found among beef Bos indicus and Bos taurus cows maintained in a subtropical environment (Alvarez P et al. 2000 J. Anim. Sci. 78, 1291-1302). The aim of this study was to compare ovarian response to superovulation and embryo production of tropical-adapted Bos taurus and Bos indicus cows. The experiment was carried out in a tropical wet climate at the experimental station of Instituto de Zootecnia (latitude 22°46′S, longitude 47°17′W) from November to February (average maximum temperature = 30.0 ± 0.8°C and average absolute precipitation = 153.1 ± 78.8 mm3). Forty Caracu (a local Bos taurus breed) and 50 Nelore (Bos indicus breed) lactating cows were treated with an intravaginal device containing progesterone (1.38 mg; CIDR-B®, Pfizer Animal Health, Montreal, Québec, Canada) and 2.5 mg i.m. of estradiol benzoate (Estrogin®, Farmavet, São Paulo, Brazil). Four days later, the animals were superovulated with multiple i.m. injections of 400 IU of FSH (Pluset®, Calier, Spain) in decreasing doses (75-75, 75-50, 50-25, and 25-25 IU) at 12-h intervals over 4 days. The CIDR-B® device was removed 3 days after the first superovulatory injection and cows received i.m. 150 μg of cloprostenol (Veteglan®, Calier, Spain). Cows were inseminated 48 and 62 h after the cloprostenol injection and embryos were recovered nonsurgically 7 days after insemination. Differences in the number of CL (assessed by ultrasound scanning), total number of ova/embryos, and number of transferable embryos were analyzed by ANOVA. Differences in the number of animals with low response (<3 CL) to superovulation were analyzed by chi-square test. All donors (with the exception of 1 Caracu and 2 Nelore) with ovarian response >3 CL showed estrus at insemination. Three (8.9%) Caracu and 5 (10.0%) Nelore cows had <3 CL following the superovulation treatment (P = 0.68). There was no difference (P > 0.05) in the mean (± SEM) CL counts of Caracu (11.4 ± 3.3) and Nelore (12.0 ± 4.1) cows. Similarly, there were no differences (P > 0.05) between Caracu and Nelore cows for total number of ova/embryos collected (8.6 ± 2.6 v. 9.0 ± 4.3) or transferable embryos (6.0 ± 2.4 v. 5.1 ± 2.9). In conclusion, the superovulation of Caracu and Nelore cows carried out in a tropical climate resulted in similar ovarian responses and embryo production. Supported by FAPESP.

scholarly journals Effect of pFSH dose reduction on in vivo embryo production in Dorper ewes

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4215 ◽  
Author(s):  
João Bosco Loiola Filho ◽  
Alane Pains Oliveira do Monte ◽  
Thais Thatiane Dos Santos Souza ◽  
Mayara De Souza Miranda ◽  
Lívia Correia Magalhães ◽  
...  
Keyword(s):  
Dose Reduction ◽  
Fertilization Rate ◽  
Corpora Lutea ◽  
Device Removal ◽  
Embryo Production ◽  
Chi Square ◽  
Estrus Detection ◽  
Chi Square Test ◽  
Intravaginal Device

<p>To evaluate the effect of pFSH dose on the <em>in vivo </em>embryo production of Dorper ewes in the semi-arid northeast of Brazil, 40 sheep females were distributed into two groups of 20 animals that received intravaginal CIDR for 14 days, and two days before device removal, they received one of the following treatments: in the FSH200 group, the ewes received 200 mg of pFSH; and in the FSH128 group, the ewes received a total of 128 mg in decreasing doses every 12 h. Beginning 12 h after the conclusion of the treatments, estrus detection was performed every four hours using two Dorper rams of proven fertility. The ewes were mated at estrus onset and 24 hours later. Seven days after intravaginal device removal, the superovulatory response was evaluated, and embryo collection was performed using the laparotomy method. The recovered flushings were subjected to embryo searches under a stereomicroscope and classified according to their qualities. Analyses of variance (ANOVAs) and LSD tests were used to compare the different parameters. The data expressed as percentages were analysed by chi-square test. The ovulation rate was higher in the FSH200 group, which had 16.3 ± 0.3 corpora lutea (CL), than in the FSH128 group, which had 11.3 ± 0.3 CL (P&lt;0.05). However, higher fertilization rate (83.6% vs. 62.4%) and higher transferable (86.0% vs. 71.6%) and freezable (67.9% vs. 40.8%) embryo rates were observed in the FSH 128 group compared with the group that received 200 mg. Furthermore, no significant differences in the remaining parameters were observed between the experimental groups (P&gt;0.05), demonstrating that pFSH dose reduction promoted a greater production of freezable and transferable embryos in Dorper ewes subjected to MOET.</p>

scholarly journals 317 EFFECT OF TIME OF ONSET OF FOLLICLE-STIMULATING HORMONE TREATMENT ON SUPEROVULATORY RESPONSE AND EMBRYO YIELD OF SANTA INES EWES DURING BREEDING SEASON

2013 ◽  
Vol 25 (1) ◽  
pp. 306
Author(s):  
M. E. F. Oliveira ◽  
C. C. D'Amato ◽  
C. S. Oliveira ◽  
F. F. P. C. Barros ◽  
A. P. Perini ◽  
...  
Keyword(s):  
New York ◽  
Breeding Season ◽  
Animal Health ◽  
Hormone Treatment ◽  
Corpora Lutea ◽  
Mating Period ◽  
Follicular Wave ◽  
Embryo Yield ◽  
Pfizer Animal Health ◽  
Santa Inês

This study was designed to investigate if the time of onset of FSH treatment [near the emergence of first or last follicular wave on progesterone (P4) protocol] influenced the superovulatory response and embryo yield in Santa Ines ewes during breeding season. Days of emergence of the follicular waves were defined in a previous study that evaluated the follicular dynamic in oestrus synchronization treatments (Oliveira et al. 2011 Acta Sci. Vet. 40). We observed emergence of the first and last follicular wave on 5.69 ± 0.42 and 11.25 ± 0.39 days of protocol, respectively. Twenty Santa Ines ewes were submitted to 2 superovulatory protocols according to the time that FSH treatments were initiated (G-first wave, n = 10; G-last wave, n = 10). On Day 0, all ewes received a P4 device (CIDR®; Pfizer Animal Health, New York, NY, USA) and injection of 37.5 µg of d-cloprostenol, IM. The FSH treatments started on Day 6 and Day 11 of protocol for G-first and G-last, respectively. The superovulatory regimen consisted of 8 IM injections of pFSH administrated twice daily (40, 40, 30, 30, 20, 20, 10, and 10 mg of pFSH). The P4 device was removed on Day 8 and Day 13 for G-first and G-last, respectively. At these times, all ewes received another injection of 37.5 µg of d-cloprostenol and a dose of 200 IU of eCG. During 4 days after the P4 device removal, ewes were mated by a fertile ram. Embryo collections were accomplished 7 days after CIDR withdrawal. The ovaries were evaluated by ultrasonography (3 times daily, during the mating period) and laparotomy (concomitantly with embryo collection). The superovulatory response was observed by classified by score: 0 = 4 or fewer corpora lutea (CL); 1 = between 5 and 10 CL; and 2 = 11 or more CL. Data were analysed by GLIMMIX using SAS software (SAS Institute Inc., Cary, NC, USA). All donors from G-first had superovulatory response classified as score 2, whereas 60% of ewes from G-last were classified as score 2, 20% as score 1, and 20% as score 0 (P < 0.05). There were effects between treatments (P < 0.05) for ovulation rate (G-first: 97.9 ± 1.4% v. G-last: 88.5 ± 4.4%) and number of ovulations (G-first: 17.0 ± 2.3 v. G-last: 12.5 ± 2.6). The numbers of luteinized unovulated follicles were 0.7 ± 0.5 for G-first and 1.2 ± 0.4 for G-last (P > 0.05). There was no difference between G-first and G-last (P > 0.05) in the rate of ova/embryos recovered (54.9 ± 5.7% v. 49.3 ± 8.5%), mean number of ova/embryos recovered (9.0 ± 1.4 v. 6.3 ± 1.1), number of viable embryos (3.8 ± 1.5 v. 3.4 ± 0.8), or viability rate (40.3 ± 10.8 v. 53.4 ± 12.1). In conclusion, the FSH treatment started near the emergence of the first follicular wave of progesterone protocol in Santa Ines ewes during the breeding season resulted in a higher superovulatory response than that started near the last follicular wave; however, no improvements in embryo yield were observed. Financial support: FAPESP.

scholarly journals 172 REUSE OF AUTOCLAVED INTRAVAGINAL PROGESTERONE DEVICE TO ESTROUS SYNCHRONIZATION IN TOGGENBURG GOATS IN THE BREEDING SEASON

2011 ◽  
Vol 23 (1) ◽  
pp. 188
Author(s):  
J. M. G. Souza ◽  
C. A. A. Torres ◽  
M. C. Silva ◽  
A. L. R. S. Maia ◽  
J. H. Bruschi ◽  
...  
Keyword(s):  
Breeding Season ◽  
Disease Transmission ◽  
Body Condition Score ◽  
Animal Health ◽  
Treated Group ◽  
Chi Square ◽  
New Device ◽  
Estrous Synchronization ◽  
Pfizer Animal Health ◽  
New Devices

Reusing intravaginal devices represents an important alternative to reduce costs; however, this practice may increase disease transmission. The aim of this study was to evaluate the efficacy of reusing autoclaved intravaginal progesterone devices for oestrous synchronization in Toggenburg goats in the breeding season was studied. This study was done in March and April of 2009, in Piau, MG, Brazil (latitude 21°35′ and longitude 43°15′). Sixty-seven Toggenburg nulliparous (n = 17; 35.3 ± 5.4 kg and 3.3 ± 0.2) and pluriparous (n = 50; 52.9 ± 9.8 kg and 3.4 ± 0.3) goats were assigned according to weight and body condition score (BCS, 1 to 5 scale) into 3 treatments. Animals received new devices (n = 25; 48.2 ± 11.5 kg and BCS 3.4 ± 0.3) containing 0.33 g of progesterone (Eazi-Breed CIDR®, Pfizer Animal Health, São Paulo, Brazil) or autoclaved (121°C, 1 atm, 15 min) devices previously used for 6 days (n = 23; 48.3 ± 13.0 kg and 3.5 ± 0.3) or 12 days (n = 22; 48.2 ± 11.0 kg and 3.4 ± 0.3). All goats received 5 mg dinoprost (Lutalyse®, Pfizer Animal Health) in the vulvar submucosa on the day of CIDR insertion (Day 0) and 200 IU eCG (Novormon 5000®, Sintex Indústria Bioquímica, Buenos Aires, Argentina) 1 day before CIDR removal, also in the vulvar submucosa. The CIDR were removed on day 6, and goats were bred twice daily with fertile bucks at oestrous onset and 24 h later if they were still in oestrus. Parametric variables were analysed by ANOVA and SNK tests by the SAEG® program. Nonparametric variables were analysed using the chi-square test by the BioEstat® program. The results are described as mean ± SD. Oestrous response and conception rates did not differ (P > 0.05) among goats treated with the new devices (75.0; 54.2%) or those previously used for 6 (81.8; 50.0%) or 12 days (71.4; 47.6%), respectively. No differences were detected between nulliparous (82.3; 52.9%) and pluriparous (72.0; 50.0%) goats. The interval from device removal to oestrus and duration of oestrus were not different (P > 0.05) among animals receiving a new device (39.3 ± 15.8; 30.7 ± 16.6 h) or previously used devices for 6 (32.7 ± 11.5; 31.8 ± 7.3 h) or 12 day (40.8 ± 20.7; 32.8 ± 13.2 h) treatments, respectively, or between nulliparous (41.6 ± 14.9; 30.9 ± 13.9 h) and pluriparous (35.6 ± 16.8; 32.1 ± 12.8 h) goats. Since no differences were detected in the evaluated variables among goats receiving reused autoclaved devices or new ones, it can be suggested that the autoclaving process did not affect the efficiency of reusing intravaginal progesterone devices for oestrous synchronization in Toggenburg goats in the breeding season. Probably, P4 concentrations in goats receiving reused autoclaved devices reached at least minimum concentrations to promote oestrous response, since a non-treated group would not show oestrus in this level of synchronization as in goats in this study. This technique can be a simple and valuable tool to reduce sanitary risks of disease transmission without altering fertility in goats. CNPq, Pfizer Animal Health, Embrapa Goats, and Sheep Research, CAPES.

226 INCLUSION OF BOVINE SOMATOROPIN ON MULTIPLE-OVULATION EMBRYO TRANSFER TREATMENTS FOR LOW-RESPONDING BEEF DONOR COWS

2012 ◽  
Vol 24 (1) ◽  
pp. 225
Author(s):  
S. Kmaid ◽  
J. M. Saldaña ◽  
Z. Ramos ◽  
R. Ungerfeld
Keyword(s):  
Animal Health ◽  
Control Treatment ◽  
Embryo Production ◽  
Multiple Ovulation ◽  
Oestradiol Benzoate ◽  
Pfizer Animal Health ◽  
Universal Laboratory ◽  
To Receive ◽  
Test Treatment ◽  
Donor Cows

An experiment was designed to determine if embryo production of Angus donors could be improved by including a single dose of bovine somatotropin (bST) before starting the superovulatory treatment (SPO). Thirty-three multiparous Angus cows (body condition = 4.6 ± 0.6, range = 1–8) were submitted to 2 SPO treatments at random stages of the oestrous cycle in a crossover design (60 days apart). On Day 0, cows were divided at random to receive either 500 mg of bST (n = 18; Lactotropin, Elanco Saude Animal, Brasil) or remain without bST (control, n = 15). At the same time, all cows received 2 mg of oestradiol benzoate, 50 mg of progesterone and a CIDR (Pfizer Animal Health, Uruguay). On Day 2, all cows received 200 IU of eCG (Inducel, Universal Laboratory, Uruguay) and on Day 4 were superstimulated with 425 IU of FSH (Pluset, Calier, Spain) in twice-daily decreasing doses over 4 days. On Day 6, all cows received 2 doses (a.m. and p.m.) of a PGF analogue (800 μg, Delprostenate, Glandinex, Universal Laboratory, Uruguay) and the CIDRs were removed in the morning of Day 7. Cows were injected with 10 μg of GnRH (Receptal, Intervet, the Netherlands) on Day 8 (a.m.) and were inseminated 12, 24 and 36 h later. On Day 15, ova/embryos were collected nonsurgically and evaluated in accordance to IETS guidelines. For statistical analysis, cows were categorized according to the number of transferable embryos obtained in control treatment (without bST) in terciles: low-responding cows (LR, ≤6; n = 11), middle-responding cows (7–9, n = 12), or high-responding cows (HR ≥ 10; n = 10). Data were analysed by a paired t-test. Treatment with bST increased the total ova and embryos and the number of transferable embryos in LR cows (Table 1). However, the number of transferable embryos and the percentage of transferable embryos werereduced in HR cows. The inclusion of a single bST dose 4 days before initiating SPO with FSH treatments may be a useful alternative to improve embryo production in low-responding cows. Table 1.Response of low-responding and high-responding Angus cows to superovulatory treatments with or without bST (mean ± SD)

212 EFFECT OF A LOW DOSE OF eCG ON SUPEROVULATION AND EMBRYO COLLECTION IN WOOD BISON DURING THE BREEDING SEASON

2014 ◽  
Vol 26 (1) ◽  
pp. 220
Author(s):  
J. M. Palomino ◽  
R. J. Mapletoft ◽  
M. R. Woodbury ◽  
M. P. Cervantes ◽  
G. P. Adams
Keyword(s):  
Breeding Season ◽  
Low Dose ◽  
Transvaginal Ultrasound ◽  
Animal Health ◽  
Ovulation Rate ◽  
Corpora Lutea ◽  
Chi Square ◽  
Chi Square Test ◽  
Wood Bison ◽  
Follicular Response

In an effort to conserve a threatened Canadian species, Bison bison athabascae, we developed an embryo collection protocol subsequent to superovulatory treatment involving two doses of FSH diluted in hyaluronan given 48 h apart. The follicular response to superstimulatory treatment was satisfactory, but many follicles did not ovulate, thus limiting the number of embryos collected. Based on recent results in cattle, where replacement of the final doses of FSH with a low dose of eCG resulted in the recovery of a greater number of ova/embryos, the objective of the present study was to evaluate the effect of adding eCG to the superovulatory protocol to increase ovulation rate, embryo collection, and embryo quality in wood bison during the breeding season (September). Ovarian synchronization was induced in wood bison (n = 24) by treatment with a luteolytic dose of prostaglandin (500 mcg, Cloprostenol) followed 8 days later by transvaginal ultrasound-guided follicular ablation. Follicular wave emergence (Day 0) was defined as the day after follicle ablation. Bison were assigned randomly to two groups: FSH (n = 12) and FSH+eCG (n = 12). FSH was diluted in hyaluronan (5 mg mL–1, MAP-5, Bioniche Animal Health, Belleville, ON, Canada) and given intramuscularly on Day 0 (300 mg) and Day 2 (100 mg) in both groups. Bison in the FSH+eCG group received 450 IU eCG (Pregnecol, Bioniche Animal Health) intramuscularly on Day 3, and bison in both groups were administered a luteolytic dose of prostaglandin on Day 3. On Day 5, bison were given 2500 IU hCG (Chorulon, Merck Animal Health, Summit, NJ, USA) intramuscularly to induce ovulation. The bison were artificially inseminated with chilled semen 12 and 24 h after hCG treatment. Nonsurgical embryo collection was performed on Day 13. The ovaries were examined by transrectal ultrasonography on Days 5, 7, and 13 to record the follicular response, ovulation rate, and number of corpora lutea (CL), respectively. Results were compared between groups by t-test or chi-square test (Table 1). The number of ovulatory-sized follicles (≥9 mm), ovulation rate (number ovulations/ovulatory-sized follicles), number of CL, number of ova/embryos, and number of transferable embryos were not different between groups (P = 0.23, P = 0.19, P = 0.25, P = 0.18, P = 0.09, respectively). In conclusion, the superovulatory response and embryo collection rate in wood bison approached that observed in cattle, but were not improved by the addition of a low dose of eCG. Table 1.Response of wood bison (mean ± s.e.m.) to superovulatory treatment with or without eCG

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