363 EFFECT OF OESTRADIOL VALERATE OR BENZOATE ON INDUCTION OF A NEW FOLLICULAR WAVE EMERGENCE IN BOS INDICUS COWS AND HEIFERS TREATED WITH NORGESTOMET AURICULAR IMPLANT

2006 ◽  
Vol 18 (2) ◽  
pp. 289 ◽  
Author(s):  
M. F. de Sa Filho ◽  
E. L. Reis ◽  
H. Ayres ◽  
L. U. Gimenes ◽  
A. A. C. Peres ◽  
...  
Keyword(s):  
Bos Indicus ◽  
Variable Interval ◽  
Estradiol Benzoate ◽  
Recommended Dose ◽  
Estradiol Valerate ◽  
Corpora Lutea ◽  
Dominant Follicle ◽  
Estradiol Treatment ◽  
Follicular Wave ◽  
Animal Category

Estradiol given during progestin-treatment promotes emergence of a new follicular wave in cattle. The interval from estradiol treatment to follicular wave emergence is dependent on the estradiol preparation, the dose, and possibly the animal category (cows or heifers). Therefore, this experiment aimed to compare the effects of either estradiol benzoate (EB) or estradiol valerate (EV) on the synchrony of ovarian follicular wave emergence in Bos indicus (Nelore) heifers and cows treated with a Norgestomet implant (Crestar�; Intervet, Boxmeer, The Netherlands; factorial 3 � 2 design). Lactating cows (n = 30; 70-90 days postpartum) and cycling heifers (n = 29) were homogeneously assigned, according to the presence of corpora lutea, to six experimental groups (G2EB, G2.5EV, and G5EV for each animal category). At random stages of the estrous cycle (Day 0), the females received an auricular implant and were assigned to receive 2 mg of EB (Farmavet, S�o Paulo, Brazil; 10 cows and 10 heifers; G2EB), 2.5 mg of EV plus 1.5 mg of Norgestomet (10 cows and 10 heifers; G2.5EV; half of a recommended dose), or 5 mg of EV plus 3 mg of Norgestomet (10 cows and 10 heifers; G5EV, full recommended dose) i.m. Transrectal ultrasonography was performed once daily from Day 0 to confirm the follicular dominance (new growing dominant follicle >8 mm in diameter). The day of wave emergence was retrospectively defined as the day when the dominant follicle was first detected at a diameter of 3 mm. Two-way analysis of variance was used to compare the effects of treatments (G2EB, G2.5EV, and G5EV), category (cows and heifers), and the treatments-by-category interaction on the interval from beginning of the treatment to wave emergence. The LSD was used to locate differences among groups and the Bartlett's test revealed heterogeneity of variance. Statistical analysis was conducted by SAS for Windows (SAS Institute, Inc., Cary, NC, USA). There was a significant effect of treatment and category (P < 0.001), but there were no treatments-by-category interactions (P > 0.05). A new follicular wave was induced in all animals after treatment with EB or EV. The heifers given G2EB (2.50 � 0.19a; 2 to 3x days) had a reduced (P < 0.01) and least variable interval (P < 0.05) than those given G2.5EV (4.20 � 0.27b; 3 to 6xy days) or G5EV (6.10 � 0.57c; 3 to 8y days). The cows given G5EV (4.00 � 0.52b; 2 to 6y days) had the longest (P = 0.05) and more variable interval (P < 0.001) than those given G2EB (2.50 � 0.22a; 2 to 4x days), but were similar to those given G2.5EV (3.10 � 0.41ab; 2 to 6xy days). The effect of EB was similar in heifers and cows (P > 0.05); however, the EV or 2.5EV promoted a longer emergence wave interval in the heifers than in the cows (P < 0.05). Analyzing the main effects, cows and heifers given G2EB (2.50 � 0.15a; 2 to 4x days) had a smaller and less variable interval than those given G2.5EV (3.65 � 0.27b; 2 to 6y) or G5EV (5.05 � 0.44c; 2 to 8z). In conclusion, the EV treatment, in both doses (5 or 2.5 mg), resulted in a delayed and more variable interval to emergence of a new follicular wave than 2 mg of EB in Bos indicus cows and heifers treated with a Norgestomet auricular implant. This work was supported by Intervet, Brazil.

235 FOLLICULAR WAVE EMERGENCE AND DEVELOPMENT FOLLOWING ESTRADIOL PLUS PROGESTERONE AT A GROWING OR A STATIC PHASE OF THE DOMINANT FOLLICLE IN CIDR-TREATED, LACTATING DAIRY COWS

2006 ◽  
Vol 18 (2) ◽  
pp. 225
Author(s):  
U. H. Kim ◽  
G. H. Suh ◽  
I. H. Kim
Keyword(s):  
Dairy Cows ◽  
Estradiol Benzoate ◽  
Control Group ◽  
Corpora Lutea ◽  
Dominant Follicle ◽  
Exact Test ◽  
Follicular Wave ◽  
Lactating Dairy Cows ◽  
Array Transducer ◽  
Static Phase

Treatment with estradiol benzoate (EB), compared to GnRH, in a controlled internal drug release (CIDR)-based timed AI protocol induced relatively asynchronous follicular wave emergence, resulting in ovulation of smaller ovulating follicles (Kim et al. 2005 Theriogenology 63, 260-268). In this study, we compared new follicular wave emergence and development following treatment with EB (2 mg) plus progesterone (50 mg) at a growing (Day 3 after ovulation) or a static (Day 8) phase of the dominant follicle during the first follicular wave in CIDR-treated, lactating dairy cows. Previously synchronized, lactating dairy cows received CIDRs (InterAg, Hamilton, New Zealand; Day 0) 3 days after ovulation and were assigned to two treatment groups and a control group. Cows in the T1 group (n = 11) received 2 mg EB (SY Esrone; Samyang, Seoul, Korea) and 50 mg P4 (SY Ovaron; Samyang) i.m., and those in the control group (n = 10) received no treatment at that time. Other previously synchronized cows received a CIDR (Day 0) with injections of 2 mg EB plus 50 mg P4 i.m. 8 days after ovulation (T2 group, n = 10). Thereafter, all cows received PGF2� (Lutalyse; Pharmacia & Upjohn, Puurs, Belgium) at the time of CIDR removal on Day 7, and GnRH (Conceral; Dongbang Co., Seoul, Korea) on Day 9. Ovaries of each cow were examined by transrectal ultrasonography (Sonoace 600 with 7.5 MHz linear-array transducer; Medison Co., Ltd, Seoul, Korea) every 24 h from Days 0 to 9, on Day 11, and on Day 14 of treatment to observe changes in ovarian structures (follicles and corpora lutea (CL)). Data were analyzed by Fisher's exact test, Kruskal-Wallis test, and ANOVA using an SAS program (SAS Institute, Inc., Cary, NC, USA). Follicular wave emergence occurred within 7 days in 3/10 of the control group, 9/11 of the T1 group, and 9/10 of the T2 group (P < 0.05). The interval to wave emergence did not differ (P > 0.05) among groups (3.7 � 1.2, 4.8 � 0.5, 5.9 � 0.3 days, respectively). While the diameters of dominant follicles on Day 0 were greater (P < 0.01) in the T2 group (14.3 � 1.2 mm) than in the control (9.4 � 0.6 mm) and T1 (10.5 � 0.7 mm) group, diameters of preovulatory follicles on Day 9 were significantly smaller (P < 0.01) in the T1 (13.6 � 0.7 mm) and T2 (12.3 � 0.5 mm) groups than in the control group (16.4 � 0.8 mm). Synchronized ovulation by 40 h after the GnRH injection (Day 11) occurred in 10/10 of the control group, 10/11 of the T1 group, and 9/10 of the T2 group (P > 0.05). In conclusion, administration of 2 mg EB plus 50 mg P4 at both a growing and a static phase of the dominant follicle during the first follicular wave results in synchronous follicular wave emergence and development and also synchronized ovulation in CIDR-treated, lactating dairy cows.

287 EFFECT OF FOLLICULAR WAVE SYNCHRONIZATION AND ABSENCE OF CORPUS LUTEUM ON COC RECOVERY IN Gyr (BOS TAURUS INDICUS) COWS

2010 ◽  
Vol 22 (1) ◽  
pp. 300
Author(s):  
T. Miyauchi ◽  
C. A. C. Fernandes ◽  
E. R. Oliveira ◽  
B. F. L. Alves ◽  
J. H. M. Viana
Keyword(s):  
Bos Taurus ◽  
Animal Health ◽  
Cumulus Cell ◽  
Estradiol Benzoate ◽  
Corpora Lutea ◽  
Portable Ultrasound ◽  
Follicular Wave ◽  
Main Technique ◽  
Oocyte Donors

Transvaginal guided follicle aspiration (TGFA) is the main technique used in Brazil to recover oocytes for in vitro embryo production (IVP) in bovine. Different protocols have been proposed to synchronize follicular emergence in oocyte donors, but most of them were developed for use in European breeds of cattle, which show many differences in ovarian physiology when compared with Zebu breeds. The aim of this study was to compare different protocols for preparation of Gyr (dairy zebu breed) oocyte donors. The TGFA were performed in a donor management facility located in Minas Gerais State, southeast Brazil. Pluriparous cycling Gir cows (n = 42) were used as donors. All cows underwent 3 treatments: G1 (control), no treatment before TGFA; G2, 2 mg of estradiol benzoate (EB) for follicular wave synchronization given i.m. 5 days before TGFA; and G3, norgestomet auricular implants given 9 days and 2 mg of EB plus 0.53 mg of cloprostenol given 5 days before TGFA. The interval between TGFA in the same donor was greater than 30 days. All procedures were made by the same technician, using a portable ultrasound device, disposable 19G or 20G needles, and a vacuum pressure of 80 mm Hg. The aspirated follicular fluid was collected in 50-mL Falcon tubes and sent to the laboratory for COC identification and classification under 50 × magnification. Recovered oocytes were classified according to cumulus cell layers and cytoplasm morphology. The total number of oocytes and viable COC recovered and the procedure length (min), including time spent for TGFA and laboratory manipulation, were compared. Data were evaluated by ANOVA, and means compared by Tukey’s test. A total of 126 TGFA sessions were performed, with recovery of 2,809 oocytes (20.31 ± 12.32 of COC and 14.83 ± 7.97 of viable COC per cow/session; mean ± SD). The total number of recovered oocytes and viable COC recovered were lower in G1 compared with G2 and G3 (15.18 ± 11.07 v. 21.18 ± 9.71 and 24.68 ± 9.03; and 9.53 ± 7.22 v. 16.97 ± 6.47 and 18.84 ± 8.90, respectively; P < 0.05) There was no difference (P > 0.05) between G2 and G3 on the number of oocytes or viable COCs recovered. The procedure length, however, was longer in G1 and G2 compared with G3 (49.6 ± 15.1 and 46.9 ± 13.4 v. 35.8 ± 13.1 min, respectively; P < 0.05) The shorter procedure length in G3 was probably associated with the reduced number of cows showing no corpora lutea (38/42, 90%), which resulted in reduction of bleeding and clot formation in the aspirated fluid. These results show that (1) previous follicular wave synchronization by EB can improve the number and quality of recovered oocytes; (2) the absence of corpora lutea does not increase the number of recovered COC and viable COC, but reduces the time spent in the procedure. Intervet Schering Plough Animal Health.

16 STRATEGIES OF FOLLICULAR WAVE SYNCHRONIZATION WITH ESTRADIOL BENZOATE IN GYR (BOS TAURUS INDICUS) CATTLE

2013 ◽  
Vol 25 (1) ◽  
pp. 155
Author(s):  
L. D. P. Sinedino ◽  
B. T. Gerhardt ◽  
J. A. Moura ◽  
A. P. Dourado ◽  
I. L. Goulart ◽  
...  
Keyword(s):  
Blood Sample ◽  
Estrous Cycle ◽  
Bos Taurus ◽  
Animal Health ◽  
Estradiol Benzoate ◽  
Wilcoxon Test ◽  
Second Step ◽  
Follicular Atresia ◽  
Follicular Wave ◽  
Animal Category

Physiological and behavioral differences between Bos taurus and Bos indicus can influence the response to a fixed AI protocol. The objective of this study was to examine the reduction of the usual dose of 2 mg of estradiol benzoate (EB) to 1 mg at the beginning of a fixed-time AI protocol, aiming at follicular regression. In a second step, we evaluated the effect of EB on follicle development during follicular pre-deviation and dominance. The experiment was performed at Monte Verde Farm (Uberaba, MG, Brazil). Twenty-two cows (n = 10 heifers and n = 12 nonlactating cows) underwent an estrous synchronization protocol with a progesterone-releasing intravaginal device (P4; Sincrogest®, Ouro Fino Animal Health, São Paulo, Brazil) and received 1 (G1mg, n = 11) or 2 mg (G2mg, n = 11) of EB (Sincrodiol®, Ouro Fino Animal Health), on a random day (designated Day 0). Follicular dynamics was monitored once per day by ultrasonography from Day 0 to 4 with blood sample collections. In a second step, females received 2 mg of EB on Day 3 (GD3, pre-deviation, n = 4) or Day 5 (GD5, dominance, n = 4) of the estrous cycle (Day 0 was the ovulation). Following these treatments, follicular development was monitored daily for 6 days with blood sample collections. The statistical analysis was conducted using the SAS System for Windows 2 (2003; SAS Institute Inc., Cary, NC, USA). The explanatory variables included in the statistical model were the dose of EB, animal category (cows and heifers), and their interaction. The mean test was used to compare intervals from EB treatment to follicular atresia and follicular wave emergence using ANOVA. Progesterone concentrations between groups were compared using the Wilcoxon test. Independently of animal category or stage of the estrous cycle, both EB doses (1 or 2 mg) induced follicular atresia in 2.2 ± 0.9 and 2.1 ± 1.2 days (P > 0.05), respectively. Emergence of a new follicular wave was observed, from Day 0 to 4, in 64% (7/11) of females from G1mg and in 45% (5/11) from G2mg, and the interval between treatment and follicular emergence was 3.4 ± 0.8 and 3.0 ± 1.0 days (P > 0.05), respectively. Plasma progesterone concentrations of the 22 animals increased from 2.1 ± 2.0 ng mL–1 to 7.6 ± 3.0 ng mL–1 by 24 h after the device insertion (P < 0.05), reaching peak concentration (8.0 ± 3.0 ng mL–1) by 48 h after treatment beginning, decreasing to 6.4 ± 2.5 ng mL–1 by 72 h, and remaining constant up to 96 h. Estradiol benzoate injection at follicle pre-deviation (GD3) caused follicular atresia (2.0 ± 1.4 days) and emergence of a new follicular wave in 3.7 ± 0.1 days in all animals (4/4). However, EB injection during follicle dominance (GD5) did not synchronize a new follicular wave and follicles persisted during the time of monitoring. Furthermore, EB applied at dominance hastened luteolysis in 50% (2/4) of the treated animals. In conclusion, a reduced dose of EB (1 mg) at the beginning of the protocol with P4 effectively induces follicular atresia. To synchronize a wave emergence at any stage of the estrous cycle, EB must be associated with an exogenous source of progesterone.

scholarly journals Comparison of three doses of estradiol benzoate for synchronization of follicular wave emergence in suckled Bos indicus beef cows

2021 ◽  
Vol 18 (3) ◽  
Author(s):  
Amanda Guimarães Silva ◽  
Leonardo Marin Ferreira Pinto ◽  
Nadark de Amorim Silva ◽  
Ana Clara Degan Mattos ◽  
Pablo Henrique Ambrósio ◽  
...  
Keyword(s):  
Bos Indicus ◽  
Beef Cows ◽  
Estradiol Benzoate ◽  
Follicular Wave

418 EFFECT OF SUPEROVULATION INITIATED AT FOLLICULAR WAVE EMERGENCE IN Santa Inês EWES

2010 ◽  
Vol 22 (1) ◽  
pp. 366
Author(s):  
B. D. M. Silva ◽  
T. A. S. N. Silva ◽  
L. Dalcin ◽  
C. M. Lucci ◽  
J. P. Neves
Keyword(s):  
Total Dose ◽  
Buenos Aires ◽  
Ovarian Response ◽  
Corpora Lutea ◽  
Dominant Follicle ◽  
Treatment Groups ◽  
Follicular Wave ◽  
Preliminary Study ◽  
Santa Inês ◽  
Fresh Semen

Superovulation in ewes has been a source of many studies aimed at obtaining high superovulatory response and number of viable embryos recovered. In a protocol called Day 0, superovulatory treatment was initiated at the time of wave emergence in the absence of a dominant follicle (Menchaca A et al. 2002 Theriogenology 58, 1713-1721). The aim of this study was to compare ovarian response and number of embryos recovered after treatment between ewes treated with a Day 0 protocol and those treated with a traditional protocol. Santa InÊs ewes (n= 18) between 2 and 5 years old were randomly distributed into 2 superovulation treatment groups: traditional protocol and Day 0 protocol. Each treatment was repeated twice in a crossover model. The traditional protocol consisted of the intravaginal insertion of a sponge containing 60 mg of medroxiprogesterone acetate (MAP; Progespon®, Tecnopec, São Paulo, Brazil) for 14 days, which was replaced on Day 7, followed by 150 μg of cloprostenol (Prolise®, Tecnopec) i.m. On Day 12, FSH (Folltropin®-V, Bioniche, Belleville, Ontario, Canada) treatment was initiated using a total dose of 200 mg, given in twice-daily i.m. injections that decreased in dose over 4 days. A dose of 200 IU of eCG (Novormon®, Syntex, Buenos Aires, Argentina) i.m. was given at the time of sponge withdrawal. The AI was done by laparoscopy at 48 and 55 h after sponge withdrawal using fresh semen. The Day 0 protocol consisted of a previous 9-day synchronization treatment with a sponge containing 60 mg of MAP, followed by 150 μ of cloprostenol i.m. and 200 IU of eCG i.m. given on Day 7. A dose of 0.05 mg of GnRH (lecirelin; Gestran Plus®, Tecnopec) i.m. was given 16 h after sponge withdrawal. In a preliminary study, 38 ewes ovulated 42 ± 52 h after sponge withdrawal. Therefore, 48 h after sponge removal was considered as Day 0 and FSH treatment was initiated at that time, with a total dose of 200 mg of Folltropin®-V, given in 6 twice-daily decreasing doses. Two doses of cloprostenol (150 μg) were given i.m. concurrent with the fifth and sixth FSH treatments. Gonadotropin-releasing hormone (0.05 mg of lecirelin i.m.) was given 12 h after the last FSH treatment. Artificial insemination with fresh semen was done by laparoscopy 16 and 26 h after GnRH treatment. Five days after AI, embryos were recovered surgically. Results were evaluated by the parametric t-test. The number of corpora lutea and ova/embryos recovered did not differ (P > 0.05) between the traditional (9.8 ± 5.3; 4.5 ± 4.6) and Day 0 (10.0 ± 6.0; 3.5 ± 4.3) protocols. Similarly, no difference in the number of viable embryos was observed between treatments (1.6 ± 2.0 and 1.7 ± 2.4 for the traditional and Day 0 treatments, respectively). Within viable embryos, the traditional protocol (0.4 ± 1.0) resulted in ahigher (P < 0.05) number of morulae than that of the Day 0 protocol (0.1 ± 0.3). The ewes that had no superovulatory response did not differ (P > 0.05) between the traditional (11.11%) and Day 0 (5.56%) protocols. In summary, there was no difference in the parameters evaluated between both protocols. Financial support: FAPDF, CNPq, Tecnopec.

scholarly journals 15EFFECT OF DOSE OF ESTRADIOL VALERATE ON OVARIAN FOLLICULAR DYNAMICS IN CIDR-TREATED BEEF COWS

2004 ◽  
Vol 16 (2) ◽  
pp. 130 ◽  
Author(s):  
R.J. Mapletoft ◽  
M.G. Colazo ◽  
J.A. Small ◽  
D.R. Ward ◽  
J.P. Kastelic
Keyword(s):  
Ovarian Follicle ◽  
Animal Health ◽  
Beef Cows ◽  
Estradiol Valerate ◽  
Control Group ◽  
Dominant Follicle ◽  
Follicular Wave ◽  
Follicle Diameter ◽  
Growth Profiles ◽  
Follicular Dynamics

The objective was to investigate the effect of dose of estradiol valerate (EV) on ovarian follicular growth profiles, intervals to follicular wave emergence and, following CIDR removal, estrus and ovulation in beef cows. On Day 0, 43 non-lactating, crossbred beef cows, 3 to 9 yr of age and at random stages of the estrous cycle, received a CIDR (Bioniche Animal Health; Belleville, Ontario, Canada) and were randomly allocated to one of four groups to receive no further treatment (Control; n=10), or an injection of 1mg (n=11), 2mg (n=10), or 5mg (n=12) i.m. of EV (Sigma Chemical Co, St. Louis, MO, USA) in 2mL canola oil. On Day 7, CIDR were removed and cows received 500μg i.m. of cloprostenol (Estrumate, Schering Plough Animal Health, Pointe-Claire, Quebec, Canada). Ovaries were examined by transrectal ultrasonography once daily until 48h after CIDR removal to detect ovarian follicle growth profiles, and twice daily thereafter to detect ovulation. Data were analyzed by ANOVA (LSD and Bartlett’s tests) and chi-square procedures. One cow (5mg EV group) lost the CIDR and was removed from all analyses. There was an effect of day (P&lt;0.0001) on CL diameter, but the effects of treatment (P=0.3), and the treatment-by-day interaction (P=0.1), were not significant. Follicular wave emergence occurred within 7d in 7/10 (70%) Control cows and 31/32 (97%; P&lt;0.04) EV-treated cows (one cow in late diestrus at the time of treatment did not respond to 1mg EV). Mean (±SD) interval from treatment to wave emergence was longer (P&lt;0.03) in cows treated with 5mg EV (4.8±1.2d) than in those treated with 1mg (3.2±0.9 days) or 2mg EV (3.4±0.8 days), while Control cows were intermediate (3.8±2.0 days). Although follicular wave emergence tended (P&lt;0.09) to be more synchronous in cows receiving EV, intervals from CIDR removal to estrus (P=0.7) and ovulation (P=0.8) did not differ among groups. Diameter of the dominant follicle was smaller (P&lt;0.04) at CIDR removal and tended to be smaller (P&lt;0.08) just prior to ovulation in the 5mg EV group (8.5±2.2 and 13.2±0.6mm, respectively) than in the Control (11.8±4.6 and 15.5±2.9mm, respectively) or 1mg EV (11.7±2.5 and 15.1±2.2mm, respectively) groups, with the 2mg EV group (10.7±1.5 and 14.3±1.7mm, respectively) intermediate. Diameter of the dominant follicle at CIDR removal was less variable (P&lt;0.01) in the 2 and 5mg EV groups than in the Control group and intermediate in the 1mg EV group. In summary, dose of EV affected follicular dynamics, interval to and synchrony of follicular wave emergence, and dominant follicle diameter at CIDR removal and just prior to ovulation in CIDR-treated cows. However, interval from CIDR removal to estrus and ovulation was not affected by treatment. Results suggest that a dose of 2mg EV may be most efficacious in synchronizing follicular wave emergence in CIDR-treated cows.

scholarly journals PSXIII-39 Onset of ovulation in suckled cows treated with progesterone and estradiol benzoate

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 377-377
Author(s):  
Giovanni M Baez ◽  
Wilfran E Rivera
Keyword(s):  
Corpus Luteum ◽  
Bos Indicus ◽  
Estradiol Benzoate ◽  
Control Group ◽  
Dominant Follicle ◽  
Crossbred Cows ◽  
Cow Calf ◽  
Ovarian Cyclicity ◽  
And Control ◽  
Intravaginal Device

Abstract Tropical cow-calf operations using Bos indicus crossbreds are characterized by extended periods of time before the establishment of ovarian cyclicity. The aim was to determine whether the increase of progesterone concentrations during postpartum anestrous using intravaginal devices along with injection of estradiol benzoate would advance the occurrence of first ovulation after calving. 269 suckled crossbred cows kept in different farms at North-Santander (Colombia) were randomized in one of the following treatments: 1:Control (n = 88) no further treatment; 2:P4 (n = 91) cows received an intravaginal device containing 0.588 g of progesterone during 7 days at day 50±3 after calving; 3:P4+E2 (n = 90) as treatment 2 plus an additional injection of 2.0 mg of estradiol benzoate at the time of device insertion. Ovarian ultrasound was performed weekly starting on the day of treatments until ovulation (disappearance of a dominant follicle with later appearance of a corpus luteum within two consecutive examinations). Calving-Ovulation Interval was recorded and means between groups were analyzed using ANOVA (α=0.05). For the control group, COI tended (P = 0.08) to be longer for primiparous cows (149.6±4.5 days, n = 14) compared to multiparous cows (140.4±2.1d, n = 74). Treatment with P4 reduced the COI for both primiparous (105.3±5.4d, n = 15; P &lt; 0.0001) and multiparous (96.4±3.0d, n = 76; P &lt; 0.0001) compared to the controls (P = 0.21 between groups of age). When primiparous cows were treated with P4+E2 the COI decreased compared to both P4 and control treatments (82.3±2.8d, n = 17; P &lt; 0.0001). However, in multiparous cows there was no effect of P4+E2 compared to P4 treatment (94.6±2.6d, n = 73; P = 0.65), then COI was shorter for primiparous compared to multiparous cows (P = 0.03) with P4+E2 treatment. The use of an intravaginal device containing progesterone advances the time of first ovulation after calving in suckled cows. Additional use of estradiol benzoate reduces the time for first ovulation in primiparous but not multiparous cows.

scholarly journals GnRH or estradiol benzoate combination with CIDR improves in-vivo embryo production in bovines (Bos indicus and Bos taurus) under subtropics

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12077
Author(s):  
Khalid Mahmood ◽  
Muhammad Zahid Tahir ◽  
Mahboob Ahmad Butt ◽  
Shazia Mansoor Qureshi ◽  
Amjad Riaz
Keyword(s):  
Bos Taurus ◽  
Bos Indicus ◽  
Estradiol Benzoate ◽  
Limiting Factors ◽  
Control Group ◽  
Corpora Lutea ◽  
Embryo Production ◽  
Varied Response ◽  
Donor Cows

Multiple Ovulation and Embryo Transfer (MOET) technology is a potential technique to upgrade livestock species’ genetics. The varied response to super-stimulatory treatments remains one of the limiting factors to this technology’s widespread use. The present study was aimed to improve the superovulation response and in-vivo embryo production by using controlled internal drug release (CIDR)-GnRH or CIDR-EB (Estradiol Benzoate) along with conventional superovulation protocol in Holstein Frisian (HF): Bos taurus; n = 42) and Crossbred (XB: Cholistani (Bos indicus) × HF; n = 28) cows. In the CIDR-GnRH/CIDR-EB treatment, CIDR was implanted in the cows after confirming the presence of a corpus luteum (CL) on the 8th day after estrus. 2 ml GnRH (Lecirelin acetate 0.0262 mg/ml) or 2 mg EB was also administered in CIDR-GnRH/CIDR-EB groups, respectively. Both groups were given super-stimulatory treatment from the 11th day after estrus (FSH in tapering doses twice a day for four consecutive days). On day 13, two doses of 2 ml prostaglandin (75 µg/ml of dextrorotatory cloprostenol) were administered (am: pm), and CIDR was removed the following day. Two artificial inseminations (AI) of the cows were performed (12 h apart) on the 15th day. No CIDR and GnRH/E.B were given in the control group, but the remaining superovulation protocol was the same. Later on, seven days after the first AI, non-surgical embryo flushing was done. The transferable embryos produced from three different superovulation protocols were then transferred into the recipient cows (n = 90) for determining their fertility. Statistical analysis revealed that the number of super-estrus follicles (SEF), multiple corpora lutea (MCL), ovulation/fertilization percentage, fertilized structures recovered (FSR), and transferable embryos (TEs) remained significantly higher (p < 0.05), and days taken for return to estrus (RTE) after embryo collection remained significantly lower (p < 0.05) in CIDR-GnRH (n = 18) and CIDR-EB (n = 15) groups as compared to the control (n = 37). The comparison between XB and HF cows revealed that the TEs production in CIDR-GnRH (XB = 5 vs HF = 13) and CIDR-EB (XB = 6 vs HF = 9) based superovulation protocols were 11.60  ±  4.08 vs 04.31  ±  0.98 and 09.33  ±  1.78 vs 05.22  ±  1.36, respectively. TEs production in XB cows (n = 5) of the CIDR-GnRH group was significantly higher (11.60  ±  4.08) than other groups. On the other hand, the days taken for RTE after embryo collection remained significantly lower (p < 0.05) in HF cows of treatment groups. However, the fertility of TEs was neither affected significantly (p > 0.05) by the superovulation protocol used nor by breed differences among donor cows. In conclusion, using CIDR-GnRH or CIDR-EB along with conventional superovulation protocol may enhance the efficiency of MOET programs in cattle. Furthermore, XB donor cows demonstrated a better performance than HF donor cows under subtropical conditions.

scholarly journals 228DOMINANT FOLLICLE DEVIATION IN GUZERA BREED (BOS INDICUS) COWS

2004 ◽  
Vol 16 (2) ◽  
pp. 235
Author(s):  
J.H.M. Viana ◽  
G.T.R.M. Coutinho ◽  
M.P. Palhao ◽  
L.S.A. Camargo ◽  
W.F. Sa ◽  
...  
Keyword(s):  
Bos Indicus ◽  
Maximum Diameter ◽  
Zebu Cattle ◽  
Dominant Follicle ◽  
Estrous Cycles ◽  
Portable Ultrasound ◽  
Follicular Wave ◽  
Lower Maximum ◽  
Ovulatory Follicles ◽  
Follicular Dynamics

Follicular dynamics of Zebu Cattle are characterized by lower maximum diameter and persistence of dominant follicles, when compared to European breeds. However, the main effects of follicular dominance on subordinated follicle population, and consequently on oocyte recovery and embryo production, have been shown to be similar among breeds. The aim of this study was to evaluate the dominant follicle establishment period, indicated by follicle divergence, in Zebu cows. Pluriparous, non-lactating Guzera breed (Bos indicus) cows (n=5), presenting a mean body weight of 518.0±48.5kg and similar body score condition (ranging from 3.5 to 4, in a 1 to 5 scale), were used. In a first trial, follicular dynamics were evaluated daily during 1 (n=2) or 2 (n=3) estrous cycles, using a portable ultrasound device equipped with a linear rectal 5MHz transducer (Aloka SSD500, Aloka Co., Tokyo, Japan). In a second trial, estrous cycles were synchronized using progesterone intravaginal-releasing devices (CIDR, Pharmacia, Sao Paulo, Brazil), and follicular dynamics were evaluated every 6h after ovulation, during the first follicular wave, until the largest follicle achieved a diameter of 10mm. During each evaluation ovaries were mapped, and evolution of follicles followed individually. Data are normalized for the moment of follicular divergence, and analyzed by ANOVA. Results are presented as mean±SD. Data from the first evaluations confirmed the occurrence of cycles with three (4/8) and four (1/8) follicular waves, despite a mean cycle length of 19.1±1.9 days, with a maximum diameter of 11.6±2.4mm for non-ovulatory follicles, and 14.4±0.5mm for ovulatory follicles. Follicular deviation during the first follicular wave occurred 49.2±5.0h after ovulation, when the largest follicle presented a diameter of 7.3±0.6mm, and the second largest follicle 6.8±0.7mm. Growth rates of the dominant and the largest subordinated follicles were similar before (0.35mm/6h vs. 0.33mm/6h, respectively;; P&gt;0.05), but significantly different after deviation (0.52mm 6h v. −0.04mm 6h; P&lt;0.05). These results shows that the lower maximum diameter and persistence of dominant follicles, in Zebu cattle, is partially compensated by an earlier dominant follicle deviation.

Resynchronization of follicular wave using long-acting injectable progesterone or estradiol benzoate at 14 days post-TAI in Bos taurus x Bos indicus beef heifers

2021 ◽  
Author(s):  
Camila Cupper Vieira ◽  
Hirya Fernandes Pinto ◽  
Vanessa Buss ◽  
Bruno Gonzalez de Freitas ◽  
Bruna Martins Guerreiro ◽  
...  
Keyword(s):  
Bos Taurus ◽  
Bos Indicus ◽  
Estradiol Benzoate ◽  
Beef Heifers ◽  
Follicular Wave ◽  
Long Acting
Sign in / Sign up

Export Citation Format

Share Document