scholarly journals Sperm chromatin structure assay and classical semen parameters: systematic review

2010 ◽  
Vol 20 (1) ◽  
pp. 114-124 ◽  
Author(s):  
J.A. Castilla ◽  
S. Zamora ◽  
M.C. Gonzalvo ◽  
J.D. Luna del Castillo ◽  
J.A. Roldan-Nofuentes ◽  
...  
Keyword(s):  
Systematic Review ◽  
Chromatin Structure ◽  
Semen Parameters ◽  
Sperm Chromatin ◽  
Sperm Chromatin Structure Assay

scholarly journals 262 SPERM CHROMATIN STRUCTURE, OXIDATIVE STRESS AND BASIC SEMEN PARAMETERS OF MEN FROM SUBFERTILE COUPLES

2005 ◽  
Vol 17 (2) ◽  
pp. 281
Author(s):  
M. Bochenek ◽  
P. Gogol ◽  
J. Janeczko
Keyword(s):  
Chromatin Structure ◽  
Sperm Concentration ◽  
World Health ◽  
Semen Parameters ◽  
Sperm Chromatin ◽  
Sperm Chromatin Structure Assay ◽  
Progressive Motility ◽  
Fertility Potential ◽  
Health Organization ◽  
High Level

It is known that the mammalian sperm chromatin structure plays an important role in male fertility. In opposition to many other areas of biological research, the human sperm chromatin can be considered as a model for animal fertility investigations. This is due to the great number of males with high levels of chromatin abnormalities and the ease of tracking their fertility potential. The aim of the study was to find a relationship between sperm chromatin structure, level of reactive oxygen species (ROS) and the basic semen parameters: sperm concentration and motility. The semen from a total of 391 men from subfertile couples 22–51 years old was used. The sperm chromatin abnormalities were examined flow cytometrically according to the SCSA method (sperm chromatin structure assay; Evenson D.P. Methods In Cell Biology, vol. 33, 1990) and ROS level was examined by luminometry (Kolletis et al. 1999 Fertil. Steril.). Sperm concentration and motility were checked microscopically. Sperm concentration of the examined ejaculates ranged from 0.05 × 106/mL to 627.5 × 106/mL and progressive motility ranged from 0% to 70%. More than 30% of spermatozoa with abnormal chromatin (level considered as the infertility threshold) was found in 70 (17.9%) patients; 15–30% of spermatozoa with abnormal chromatin (level of decreased fertility potential) was found in 154 (39.4%) patients; and in 167 (42.7%) patients the number of abnormal spermatozoa did not exceed 15% (level of normal fertility potential; Evenson et al. 1999 Hum. Reprod.; Zini et al. 2001 Fertil. Steril.). High significant correlations were found between chromatin abnormality and: patients' age (0.1008, P = 0.017), sperm concentration (−0.2735, P < 0.001), progressive motility (−0.4365, P < 0.001), and ROS level (0.2709, P < 0.001). However in patients with normal sperm concentration (>20 × 106/mL, according to the World Health Organization), as many as 11.5% had a high level of chromatin abnormality (>30% of abnormal chromatin) and 29.7% of moderate chromatin abnormality (15–30% abnormal chromatin). Similarly, in patients with normal progressive sperm motility (>50%, according to the World Health Organization) 1.7% had a high level of chromatin abnormality (>30% of abnormal chromatin), and 33.9% had a moderate level of chromatin abnormality (15–30% abnormal chromatin). Contrary to the findings of many earlier investigations, a strong relationship between sperm chromatin damages and basic semen parameters was observed in this work. The sperm chromatin structure assay should be included in standard semen examination to avoid expensive and time consuming in vitro procedures for spermatozoa with damaged DNA.

Evaluating human sperm DNA integrity: relationship between 8-hydroxydeoxyguanosine quantification and the sperm chromatin structure assay

Zygote ◽  
2003 ◽  
Vol 11 (4) ◽  
pp. 367-371 ◽  
Author(s):  
Isabelle Oger ◽  
Christelle Da Cruz ◽  
Gilles Panteix ◽  
Yves Menezo
Keyword(s):  
Dna Fragmentation ◽  
Chromatin Structure ◽  
Inverse Relationship ◽  
Sperm Concentration ◽  
Sperm Chromatin ◽  
Sperm Chromatin Structure Assay ◽  
Fragmentation Index ◽  
Sperm Dna ◽  
Oxidative Products ◽  
Dna Fragmentation Index

In our work, we have used 8-hydroxy-deoxyguanosine (8-OH-dG), one of the major oxidative products of sperm DNA, in a population of patients consulting for infertility. We found an inverse relationship between sperm concentration and the log of the ratio of 8-OH-dG to dG (P<0.01). On the same patients' sperm samples, the sperm chromatin structure assay (SCSA) was performed. An inverse relationship was observed between the DNA fragmentation index and sperm concentration (P<0.001). There was also a positive relationship between SCSA and log 8-OH-dG/dG. This indicates that DNA fragmentation measured by the SCSA originates in part from oxidative products. In a few patients, antioxidant treatment decreased the DNA fragmentation index below the threshold of 30% that is crucial for subfertility.

Increased DNA damage in sperm from leukocytospermic semen samples as determined by the sperm chromatin structure assay

2002 ◽  
Vol 78 (2) ◽  
pp. 319-329 ◽  
Author(s):  
Juan G Alvarez ◽  
Rakesh K Sharma ◽  
Mario Ollero ◽  
Ramadan A Saleh ◽  
Mari C Lopez ◽  
...  
Keyword(s):  
Dna Damage ◽  
Chromatin Structure ◽  
Sperm Chromatin ◽  
Sperm Chromatin Structure Assay
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