Glycoconjugates as positive and negative modulators of embryo implantation

1992 ◽  
Vol 4 (3) ◽  
pp. 271 ◽  
Author(s):  
DD Carson ◽  
AL Jacobs ◽  
J Julian ◽  
LH Rohde ◽  
MC Valdizan
Keyword(s):  
Binding Proteins ◽  
Embryo Implantation ◽  
Comprehensive Understanding ◽  
Process Identification ◽  
Implantation Process ◽  
Adhesive Interactions ◽  
Multiple Stages

A variety of studies indicate that complex glycoproteins participate in or modulate adhesive interactions occurring during embryo implantation. In particular, proteoglycans and proteins that bind proteoglycans are involved at multiple stages of this process. Identification of these binding proteins and the molecular controls over glycoconjugate expression are required to develop a comprehensive understanding of the implantation process.

scholarly journals Transcriptomic Analysis of Porcine Endometrium during Implantation after In Vitro Stimulation by Adiponectin

2019 ◽  
Vol 20 (6) ◽  
pp. 1335 ◽  
Author(s):  
Nina Smolinska ◽  
Karol Szeszko ◽  
Kamil Dobrzyn ◽  
Marta Kiezun ◽  
Edyta Rytelewska ◽  
...  
Keyword(s):  
Reproductive Success ◽  
Cytokine Production ◽  
Regulatory Mechanism ◽  
Embryo Implantation ◽  
Prostaglandin Synthesis ◽  
Comprehensive Understanding ◽  
Implantation Process ◽  
The Relationship ◽  
Pathway Networks

Comprehensive understanding of the regulatory mechanism of the implantation process in pigs is crucial for reproductive success. The endometrium plays an important role in regulating the establishment and maintenance of gestation. The goal of the current study was to determine the effect of adiponectin on the global expression pattern of genes and relationships among differentially expressed genes (DE-genes) in the porcine endometrium during implantation using microarrays. Diverse transcriptome analyses including gene ontology (GO), biological pathway, networks, and DE-gene analyses were performed. Adiponectin altered the expression of 1286 genes with fold-change (FC) values greater than 1.2 (p < 0.05). The expression of 560 genes were upregulated and 726 downregulated in the endometrium treated with adiponectin. Thirteen genes were selected for real-time PCR validation of differential expression based on a known role in metabolism, steroid and prostaglandin synthesis, interleukin and growth factor action, and embryo implantation. Functional analysis of the relationship between DE-genes indicated that adiponectin interacts with genes that are involved in the processes of cell proliferation, programmed cell death, steroid and prostaglandin synthesis/metabolism, cytokine production, and cell adhesion that are critical for reproductive success. The presented results suggest that adiponectin signalling may play a key role in the implantation of pig.

Effects of low doses of mifepristone on human embryo implantation process in a three-dimensional human endometrial in vitro co-culture system

Contraception ◽  
2016 ◽  
Vol 94 (2) ◽  
pp. 143-151 ◽  
Author(s):  
N.R. Boggavarapu ◽  
C. Berger ◽  
C. von Grothusen ◽  
J. Menezes ◽  
K. Gemzell-Danielsson ◽  
...  
Keyword(s):  
Human Embryo ◽  
Culture System ◽  
Embryo Implantation ◽  
Three Dimensional ◽  
Low Doses ◽  
Implantation Process

scholarly journals Leptin Serves as an Upstream Activator of an Obligatory Signaling Cascade in the Embryo-Implantation Process

Endocrinology ◽  
2005 ◽  
Vol 146 (2) ◽  
pp. 694-701 ◽  
Author(s):  
M. P. Ramos ◽  
B. R. Rueda ◽  
P. C. Leavis ◽  
R. R. Gonzalez
Keyword(s):  
Embryo Implantation ◽  
Signaling Cascade ◽  
Implantation Process

Angiopoietin-1 and -2 mRNA and protein expression in mouse preimplantation embryos and uteri suggests a role in angiogenesis during implantation

2006 ◽  
Vol 18 (5) ◽  
pp. 509 ◽  
Author(s):  
A. P. Hess ◽  
J. Hirchenhain ◽  
A. Schanz ◽  
S. Talbi ◽  
A. E. Hamilton ◽  
...  
Keyword(s):  
Protein Expression ◽  
Embryo Implantation ◽  
Mouse Embryos ◽  
Preimplantation Embryos ◽  
Preimplantation Mouse Embryos ◽  
Preimplantation Mouse ◽  
Mrna And Protein Expression ◽  
Implantation Process ◽  
Endometrial Epithelium ◽  
Angiopoietin 1

After attachment and migration through the endometrial epithelium, the embryo must induce angiogenesis within the endometrial stroma to successfully complete the implantation process. Growth factors have been shown to play an important role in embryo implantation and placentation. The aim of the study was to investigate the expression of angiopoietin-1 and -2 (Ang-1 and -2) mRNA and protein expression during the development of single preimplantation mouse embryos and of possible complementary expression in mouse uteri. Angiopoietin-1 mRNA was expressed throughout development in 78% of zygotes, 66% of 2-cell-embryos, 71% of 4-cell-embryos, 70% of 8-cell-embryos, 60% of morula stages, 48% of early blastocysts and 78% of late blastocysts. The number of Ang-1-expressing embryos in the early-blastocyst group was significantly different in comparison with zygotes, 4-cell-embryos, 8-cell-embryos and late blastocysts. Angiopoietin-2 mRNA and protein expression could not be detected in preimplantation embryos. Examination of the uteri revealed Ang-2 mRNA and protein expression in the oestrogen-dominated cycling phase and the progesterone-dominated mated phase, whereas Ang-1 expression was restricted to the mated phase. Herein, Ang-1 expression in preimplantation mouse embryos as well as Ang-1 and -2 expression in mouse uteri is demonstrated, suggesting a possible role for angiopoietins in the embryo–maternal dialogue of the implantation process via an enhancement of the vascular remodelling in favour of an implanting conceptus.

scholarly journals Microarray analyses of closely related glycoforms reveal different accessibilities of glycan determinants on N-glycan branches

Glycobiology ◽  
2019 ◽  
Vol 30 (5) ◽  
pp. 334-345 ◽  
Author(s):  
Lei Li ◽  
Wanyi Guan ◽  
Gaolan Zhang ◽  
Zhigang Wu ◽  
Hai Yu ◽  
...  
Keyword(s):  
Binding Proteins ◽  
The Other ◽  
Comprehensive Understanding ◽  
Plant Lectins ◽  
Glycan Microarray ◽  
Comprehensive Knowledge ◽  
Analysis Platform ◽  
Microarray Analyses

Abstract Glycans mediate a wide variety of biological roles via recognition by glycan-binding proteins (GBPs). Comprehensive knowledge of such interaction is thus fundamental to glycobiology. While the primary binding feature of GBPs can be easily uncovered by using a simple glycan microarray harboring limited numbers of glycan motifs, their fine specificities are harder to interpret. In this study, we prepared 98 closely related N-glycoforms that contain 5 common glycan epitopes which allowed the determination of the fine binding specificities of several plant lectins and anti-glycan antibodies. These N-glycoforms differ from each other at the monosaccharide level and were presented in an identical format to ensure comparability. With the analysis platform we used, it was found that most tested GBPs have preferences toward only one branch of the complex N-glycans, and their binding toward the epitope-presenting branch can be significantly affected by structures on the other branch. Fine specificities described here are valuable for a comprehensive understanding and applications of GBPs.

In Vitro Tests for Detecting Chemicals Affecting the Embryo Implantation Process

2007 ◽  
Vol 35 (4) ◽  
pp. 421-439 ◽  
Author(s):  
Susanne Bremer ◽  
Eva Brittebo ◽  
Lennart Dencker ◽  
Lisbeth Ehlert Knudsen ◽  
Line Mathisien ◽  
...  
Keyword(s):  
Embryo Implantation ◽  
In Vitro Tests ◽  
Implantation Process

scholarly journals Identification of the Junctional Plaque Protein Plakophilin 3 in Cytoplasmic Particles Containing RNA-binding Proteins and the Recruitment of Plakophilins 1 and 3 to Stress Granules

2006 ◽  
Vol 17 (3) ◽  
pp. 1388-1398 ◽  
Author(s):  
Ilse Hofmann ◽  
Marialuisa Casella ◽  
Martina Schnölzer ◽  
Tanja Schlechter ◽  
Herbert Spring ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Stress Proteins ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Binding Protein ◽  
Gradient Centrifugation ◽  
Intercellular Junctions ◽  
Stress Granules ◽  
Spectrometric Analysis ◽  
Adhesive Interactions

Recent studies on the subcellular distribution of cytoplasmic plaque proteins of intercellular junctions have revealed that a number of such proteins can also occur in the cyto- and the nucleoplasm. This occurrence in different, and distant locations suggest that some plaque proteins play roles in cytoplasmic and nuclear processes in addition to their involvement in cell–cell adhesive interactions. Plakophilin (PKP) 3, a member of the arm-repeat family of proteins, occurs, in a diversity of cell types, both as an architectural component in plaques of desmosomes and dispersed in cytoplasmic particles. In immuno-selection experiments using PKP3-specific antibodies, we have identified by mass spectrometric analysis the following RNA-binding proteins: Poly (A) binding protein (PABPC1), fragile-X-related protein (FXR1), and ras-GAP-SH3-binding protein (G3BP). Moreover, the RNA-binding proteins codistributed after sucrose gradient centrifugation in PKP3-containing fractions corresponding to 25–35 S and 45–55 S. When cells are exposed to environmental stress (e.g., heat shock or oxidative stress) proteins FXR1, G3BP, and PABPC1 are found, together with PKP3 or PKP1, in “stress granules” known to accumulate stalled translation initiation complexes. Moreover, the protein eIF-4E and the ribosomal protein S6 are also detected in PKP3 particles. Our results show that cytoplasmic PKP3 is constitutively associated with RNA-binding proteins and indicate an involvement in processes of translation and RNA metabolism.

scholarly journals Role of adipokines in embryo implantation

2021 ◽  
Author(s):  
Davoud Jafari-Gharabaghlou ◽  
Mostafa Vaghari-Tabari ◽  
Hajar Oghbaei ◽  
Laura Lotz ◽  
Reza Zarezadeh ◽  
...  
Keyword(s):  
Early Pregnancy ◽  
Molecular Mechanisms ◽  
Embryo Implantation ◽  
Endometrial Receptivity ◽  
Complex Process ◽  
Strict Regulation ◽  
Implantation Process ◽  
Maternal Fetal Transmission ◽  
Multiple Molecules

Embryo implantation is a complex process in which multiple molecules acting together under strict regulation. Studies showed the production of various adipokines and their receptors in the embryo and uterus, where they can influence the maternal-fetal transmission of metabolites and embryo implantation. Therefore, these cytokines have opened a novel area of study in the field of embryo-maternal cross-talk during early pregnancy. In this respect, the involvement of adipokines has been widely reported in the regulation of both physiological and pathological aspects of the implantation process. However, the information about the role of some recently identified adipokines is limited. This review aims to highlight the role of various adipokines in embryo-maternal interactions, endometrial receptivity, and embryo implantation, as well as the underlying molecular mechanisms.

P–403 Sodium tungstate increases embryo adhesion through a direct effect on endometrial cells

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
I Canals ◽  
D Cotán ◽  
R Torres ◽  
J A Horcajadas ◽  
A Arbat
Keyword(s):  
Direct Effect ◽  
Sodium Tungstate ◽  
Embryo Implantation ◽  
In Vivo Models ◽  
Endometrial Cells ◽  
A Cells ◽  
Adhesion Rate ◽  
Implantation Process

Abstract Study question Does sodium tungstate treatment induce a change in endometrial cells’ capacity to implant trophoblasts? Summary answer Administration of sodium tungstate to endometrial cells increases trophoblast adhesion. What is known already Sodium tungstate (ST) has shown its capacity to modulate the activity of cytokines, such as leptin, an activator of an obligatory signalling cascade in the embryo-implantation process. STAT3, a signal transducer molecule critical for the embryo implantation process, is also known to be activated by ST. Still, ST’s effect on implantation using biological systems has never been studied. Embryo implantation process and endometrium roles are complicated to study in vivo due to a lack of animal models and appropriate techniques. In vitro techniques using immortalised cell lines allows a first approach to study early implantation stages, such as embryo adhesion. Study design, size, duration An in vitro study was carried out using a human endometrial carcinoma cell line (HEC–1-A) treated with sodium tungstate for 24 and 48h, and choriocarcinoma cell spheroids (JAr). Different times of treatment and concentrations were studied. Each experiment was performed in triplicate. Participants/materials, setting, methods Confluent endometrial HEC–1-A cultures were treated with ST at concentrations (0–150mM) and withaferin A (1mM), negative control for embryo adhesion. After the treatment period, HEC–1-A cultures were washed with ST-free culture medium to eliminate ST. Immediately, 15 JAr trophoblast spheroids were added to cultures and coincubated with gentle agitation for 30, 60 and 90 minutes. An inverted light microscope was used to count adhered and floating spheroids, and determine the trophoblast adherence ratio. Main results and the role of chance HEC–1-A cells treated with ST showed normal morphology and growth at all doses except 150mM. At the highest dose tested, the cells’ culture was still viable (negative blue trypan staining) and maintained morphology, but the adhesion to the plate surface was affected. Doses from 0.15 to 15mM were used to perform adhesion assays. HEC–1-A cells treated with ST for 24h showed an increased capacity to adhere JAr trophoblast spheroids. Adhesion rates reached significant differences at doses of 1.5 and 15mM after 60 and 90 minutes of coincubation. After 90 minutes, untreated cells reached 32.8% adhesion rate, while 1.5 and 15mM ST-treated cells reached 54.6% and 53.4% respectively (p &lt; 0.05 ST vs untreated). Thus, the increment of trophoblast adhesion rate induced by ST reached 66%. Lower adhesion rates were observed after 60 minutes of coincubation but were also significant with a relative increase of 49.1% at 1.5mM and 50.5% at 1.5mM when compared with untreated cells (p &lt; 0.05) Longer treatments (48h) showed similar trends to 24h-treatments, but with a lower extent of ST effect on HEC–1-A receptivity. Maximum adhesion rates were also observed at 90 minutes of coincubation and 1.5 and 15mM doses. The Mean adhesion rate increase was &gt;40% with both doses. Limitations, reasons for caution: The current study is the first approach to evaluate sodium tungstate effect on endometrium using an in vitro model. Future research using in vivo models should be performed to assess sodium tungstate effect on endometrium receptivity and its potential as a fertility treatment. Wider implications of the findings: We conclude that the direct effect of sodium tungstate on endometrial cells increases embryo adhesion rate. These results open a new research line to a potential treatment in human reproduction management with sodium tungstate to solve the unmet need of inducing embryo implantation. Trial registration number Not applicable

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