Luteinizing hormone and follicle stimulating hormone induce premature condensation of chromatin in goat (Capra hircus) oocytes

1991 ◽  
Vol 3 (5) ◽  
pp. 585 ◽  
Author(s):  
J Kumar ◽  
JC Osborn ◽  
AW Cameron
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Germinal Vesicle ◽  
Capra Hircus ◽  
High Dose ◽  
Germinal Vesicle Breakdown ◽  
Nuclear Maturation ◽  
Plasma Injection ◽  
High Doses ◽  
Stimulating Hormone

This study tested the hypothesis that premature condensation of chromatin in goat oocytes following superovulation with 1200 i.u. pregnant mare serum gonadotrophin (PMSG) is mediated by the high luteinizing hormone (LH) activity inherent in this gonadotrophin. Goats were treated with either a standard (3.95 mL) or high (7.90 mL) dose of a highly purified follicle-stimulating hormone (FSH) preparation (Ovagen), and different doses of human chorionic gonadotrophin (hCG) were added to increase the level of LH bioactivity during superovulation. The meiotic status of oocytes obtained at sponge withdrawal was compared between different treatments and correlated with profiles of LH bioactivity in peripheral plasma. Injection of 100 i.u. hCG (which gave a plasma LH profile comparable to 1200 i.u. PMSG) or 200 i.u. hCG resulted in significantly more oocytes showing premature condensation of chromatin without germinal vesicle breakdown than with 25 i.u. hCG or treatment with FSH alone. Nevertheless, nuclear maturation was also prematurely activated in a significant number of oocytes with a high dose of FSH alone, even though LH bioactivity was not detected in plasma. It is concluded that high LH bioactivity during superovulation of goats with gonadotrophins activates the initial stages of nuclear maturation in oocytes. However, highly purified FSH preparations in high doses can also induce this apparent abnormality in the timing of oocyte maturation through mechanisms unrelated to any LH contamination.

Development of a porcine follicle-stimulating hormone and porcine luteinizing hormone induced ovulation protocol in the seasonally anoestrus brushtail possum (Trichosurus vulpecula)

2002 ◽  
Vol 14 (8) ◽  
pp. 453 ◽  
Author(s):  
A. M. Glazier ◽  
F. C. Molinia
Keyword(s):  
Luteinizing Hormone ◽  
Single Injection ◽  
Polar Body ◽  
Follicle Stimulating Hormone ◽  
Brushtail Possum ◽  
Trichosurus Vulpecula ◽  
Cell Stage ◽  
Nuclear Maturation ◽  
Pregnant Mare Serum Gonadotrophin ◽  
Stimulating Hormone

Monovulatory brushtail possums (Trichosurus vulpecula) were stimulated with exogenous hormones during seasonal anoestrus to overcome ovarian insensitivity and induce ovulation. Seasonal ovarian insensitivity to pregnant mare serum gonadotrophin (PMSG) was overcome by a new porcine follicle-stimulating hormone/porcine luteinizing hormone (pFSH/pLH) protocol. This protocol was refined because the original treatment produced oocytes with abnormal morphology. Possums (n = 12 per group) received eight injections of pFSH of 1.5, 3.0 or 6.0 mg per injection (at 12-h intervals for 4 consecutive days). Ovulation was induced 12 h after the final pFSH injection with a 4-mg injection of pLH. Control animals were treated with the established protocol of a single injection of 15 IU of PMSG, followed 48 h later with an injection of 4 mg of pLH. All females responded to pFSH/pLH treatment, although optimal stimulation occurred in those receiving 8 × 3 mg pFSH, with 13–14�ovulations and recovery of 11–12 oocytes per female (8 × 1.5 mg pFSH: 13 ovulations, 8–9 oocytes; 8�×�6�mg pFSH: 7–8 ovulations, 4–5 oocytes). In contrast, only seven of 12 females responded to PMSG/pLH and, of those responding, only 2–3 ovulations occurred and only 1–2 oocytes per female were recovered. However, around 80% of oocytes recovered after PMSG/pLH treatment had undergone nuclear maturation (metaphase II/1st polar body) compared with around 60% of oocytes from pFSH/pLH-treated animals. In possums killed from 27 to 39 h after pLH treatment, ovulation onset was first observed from 30 h and by 31.5 h, all animals had completed ovulation. Laparoscopic artificial insemination (LAI) was performed on pFSH/pLH-treated animals to determine whether the oocytes produced were capable of fertilization. Uterine LAI performed 27.5–28.5 h after pLH treatment yielded 11/26 fertilized oocytes (up to 4-cell stage), whereas vaginal LAI performed 13–14 h after pLH treatment yielded 21/53 fertilized oocytes. A proportion of oocytes generated from the refined pFSH/pLH protocol are thus properly mature and capable of fertilization. Further refinement of the protocol is now needed to improve the yield of fully matured oocytes.

Premature condensation of chromatin induced in goat (Capra hircus) oocytes after gonadotrophin treatment

1990 ◽  
Vol 2 (6) ◽  
pp. 661 ◽  
Author(s):  
J Kumar ◽  
JC Osborn ◽  
AW Cameron ◽  
PA Batt ◽  
AO Trounson
Keyword(s):  
Chromatin Condensation ◽  
Germinal Vesicle ◽  
Capra Hircus ◽  
Time Intervals ◽  
Germinal Vesicle Breakdown ◽  
Treatment Groups ◽  
Expected Time ◽  
Nuclear Maturation ◽  
Significant Difference ◽  
High Incidence

In comparison with ovine follicle stimulating hormone (FSH), superovulation of goats with pregnant mare serum gonadotrophin (PMSG) produced premature ovulations within 48 h of drug administration. To test the hypothesis that this may be associated with a differential effect of the two drugs on oocyte maturation, we have compared the meiotic status of oocytes obtained at three different time intervals from animals treated with 1200 i.u. PMSG or 12 mg ovine FSH and from untreated control animals. Significantly more oocytes from PMSG-treated, compared with control and FSH-treated, animals showed premature condensation of chromatin at both the time of sponge withdrawal and 20 h later. The chromatin condensation was, however, not associated with germinal vesicle breakdown. In contrast, when oocytes were examined 6 h before the expected time of ovulation following human chorionic gonadotrophin (hCG) injection, no significant difference was found in the proportion of oocytes undergoing germinal vesicle breakdown between the three treatment groups, with most oocytes being at the metaphase I or II stage of meiosis. We conclude that superovulation of goats with PMSG at a dose resulting in a high incidence of premature ovulations is associated with premature activation of the initial stages of nuclear maturation in oocytes. In contrast, although treatment with 12 mg ovine FSH did not cause premature ovulations, it was not totally devoid of premature chromatin-condensing activity in oocytes.

ACTIONS OF MAMMALIAN, AVIAN AND PISCINE GONADOTROPHINS IN THE LIZARD

1971 ◽  
Vol 49 (1) ◽  
pp. 113-NP ◽  
Author(s):  
P. LICHT ◽  
ANNE STOCKELL HARTREE
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Anolis Carolinensis ◽  
Relative Potency ◽  
Ovarian Growth ◽  
Gonadal Activity ◽  
High Doses ◽  
Relative Potencies ◽  
Stimulating Hormone

SUMMARY The gonadotrophic activities of crude glycoprotein fractions from pituitaries of man, sheep, chicken and carp and of partially purified follicle-stimulating hormone (FSH) and luteinizing hormone (LH) from the three tetrapod species were examined in both sexes of the lizard Anolis carolinensis. The piscine material did not show activity in the lizard but all mammalian and avian preparations promoted spermatogenesis, ovarian growth, ovulation and steroidogenesis. FSH preparations were far more potent than LH, in fact the actions of the LH preparations may have been due largely to FSH contamination. These findings are consistent with earlier conclusions that FSH alone may be able to stimulate all types of gonadal activity in lizards, except that high doses of FSH block ovulation. Comparisons of the relative potencies of the avian and mammalian preparations provide evidence for the zoological specificity of vertebrate gonadotrophins. In general, the relative potency of chicken gonadotrophins in lizards was greater than that estimated from standard rodent bioassays: i.e. the lizard is relatively more sensitive to chicken gonadotrophin than the rodent.

scholarly journals Short-term effect of Tramadol injection on the serum levels of Follicle Stimulating Hormone, Luteinizing Hormone and Testosterone in Male Sahel Goats in Maiduguri, Nigeria

2019 ◽  
Vol 7 (1) ◽  
pp. 6
Author(s):  
Babagana Bako ◽  
Sani Malami ◽  
Garba Uthman Sadiq ◽  
Lawan Gana Ashiekh
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Serum Levels ◽  
Term Effect ◽  
Control Group ◽  
High Dose ◽  
Short Term ◽  
Short Term Effect ◽  
Group 4 ◽  
Stimulating Hormone

Tramadol is a synthetic analogue of codeine. Its mood elevation property and sex enhancement potentials are the main reason for its abuse. The aim of the study was to determine the short-term effect of tramadol administration on Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) and Testosterone (TEST) levels in Male Sahel Goats. This was an experimental study conducted from 1st October 2017 to 12th November 2017 at the Livestock Teaching and Research Farm, University of Maiduguri, Maiduguri, Borno State Nigeria involving 20 Male Sahel Goats. The goats were divided in to 4 groups of 5 each; group 1 served as control and groups 2, 3 and 4 were injected intramuscularly with 4 mg/kg (low dose), 8 mg/kg (medium dose) and 12 mg/kg (high dose) of Tramadol respectively. The injections were given intramuscularly, 3 times a week for 4 weeks. Blood samples were collected to determine the serum levels of FSH, LH and TEST at 0, 1 week, 2 weeks, 3 weeks and 4 weeks of tramadol injections. The Mean±SD of the hormones were computed using SPSS 20. The difference in mean was compared using t test and ANOVA with p < 0.05 set for statistical significance. The baseline levels of FSH, LH and TEST in Male Sahel Goat in Maiduguri were 2.91±5.74 Miu/ml, 0.29±0.72 Miu/ml and 3.92±6.39 ng/ml respectively. Only the goats in group 4 showed a significant increase in serum FSH and LH by the 4th week (P=0.01 and 0.03 respectively) while no significant change was noted in the other groups. The was a decline in the level of Testosterone from 1st week through 4th week in all the experimental group but the level in the control group remain fairly constant throughout the experiment. The decline is inversely proportional to the dose of tramadol injection and most marked in group 4.High dose and prolonged used of Tramadol should be avoided because of side effects of Hypergonadotrophic hypogonadism.  

scholarly journals Effects on the Secretion of Pituitary Growth Hormone, Thyroid Stimulating Hormone, Luteinizing Hormone and Follicle Stimulating Hormone in Rats Rendered Hyperprolactinaemic by Chronic Treatment with Oestrogen

1981 ◽  
Vol 34 (3) ◽  
pp. 321 ◽  
Author(s):  
GA Smythe ◽  
JF Brandstater ◽  
RF Vining
Keyword(s):  
Growth Hormone ◽  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Chronic Administration ◽  
Serum Levels ◽  
Thyroid Stimulating Hormone ◽  
Significant Inhibition ◽  
Male Rat ◽  
High Dose ◽  
Stimulating Hormone

The induction of hyperprolactinaemia in the male rat following chronic high-dose oestrogen administration over 3 months was associated With a significant inhibition of the secretion of growth hormone (OH) (P < 0�02) thyroid stimulating hormone (TSH) (P < 0�0025), luteinizing hormone (LH) and follicle stimulating hormone (FSH) (both P < 0�01). Acute, but not chronic, administration of bromocriptine (1 mg/kg) to these hyperprolactinaemic animals had the effect of normalizing the serum levels of GH and TSH but not those of LH or FSH. While the effects observed on GH, TSH, LH and FSH following induction of hyperprolactinaemia are likely to be consequential to brain actions of prolactin, the present data do not exclude the possibility of direct actions of oestrogen itself.

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