Development of a porcine follicle-stimulating hormone and porcine luteinizing hormone induced ovulation protocol in the seasonally anoestrus brushtail possum (Trichosurus vulpecula)

2002 ◽  
Vol 14 (8) ◽  
pp. 453 ◽  
Author(s):  
A. M. Glazier ◽  
F. C. Molinia
Keyword(s):  
Luteinizing Hormone ◽  
Single Injection ◽  
Polar Body ◽  
Follicle Stimulating Hormone ◽  
Brushtail Possum ◽  
Trichosurus Vulpecula ◽  
Cell Stage ◽  
Nuclear Maturation ◽  
Pregnant Mare Serum Gonadotrophin ◽  
Stimulating Hormone

Monovulatory brushtail possums (Trichosurus vulpecula) were stimulated with exogenous hormones during seasonal anoestrus to overcome ovarian insensitivity and induce ovulation. Seasonal ovarian insensitivity to pregnant mare serum gonadotrophin (PMSG) was overcome by a new porcine follicle-stimulating hormone/porcine luteinizing hormone (pFSH/pLH) protocol. This protocol was refined because the original treatment produced oocytes with abnormal morphology. Possums (n = 12 per group) received eight injections of pFSH of 1.5, 3.0 or 6.0 mg per injection (at 12-h intervals for 4 consecutive days). Ovulation was induced 12 h after the final pFSH injection with a 4-mg injection of pLH. Control animals were treated with the established protocol of a single injection of 15 IU of PMSG, followed 48 h later with an injection of 4 mg of pLH. All females responded to pFSH/pLH treatment, although optimal stimulation occurred in those receiving 8 × 3 mg pFSH, with 13–14�ovulations and recovery of 11–12 oocytes per female (8 × 1.5 mg pFSH: 13 ovulations, 8–9 oocytes; 8�×�6�mg pFSH: 7–8 ovulations, 4–5 oocytes). In contrast, only seven of 12 females responded to PMSG/pLH and, of those responding, only 2–3 ovulations occurred and only 1–2 oocytes per female were recovered. However, around 80% of oocytes recovered after PMSG/pLH treatment had undergone nuclear maturation (metaphase II/1st polar body) compared with around 60% of oocytes from pFSH/pLH-treated animals. In possums killed from 27 to 39 h after pLH treatment, ovulation onset was first observed from 30 h and by 31.5 h, all animals had completed ovulation. Laparoscopic artificial insemination (LAI) was performed on pFSH/pLH-treated animals to determine whether the oocytes produced were capable of fertilization. Uterine LAI performed 27.5–28.5 h after pLH treatment yielded 11/26 fertilized oocytes (up to 4-cell stage), whereas vaginal LAI performed 13–14 h after pLH treatment yielded 21/53 fertilized oocytes. A proportion of oocytes generated from the refined pFSH/pLH protocol are thus properly mature and capable of fertilization. Further refinement of the protocol is now needed to improve the yield of fully matured oocytes.

PREGNANT MARE SERUM GONADOTROPHIN: RATIO OF FOLLICLE-STIMULATING HORMONE AND LUTEINIZING HORMONE ACTIVITIES MEASURED BY RADIORECEPTOR ASSAY

1976 ◽  
Vol 71 (3) ◽  
pp. 371-382 ◽  
Author(s):  
FRANCESCA STEWART ◽  
W. R. ALLEN ◽  
R. M. MOOR
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Radioreceptor Assay ◽  
Trophoblast Cells ◽  
Rat Testis ◽  
A Value ◽  
Tissue Receptors ◽  
Pregnant Mare Serum Gonadotrophin ◽  
Stimulating Hormone

SUMMARY Specific radioreceptor assays for FSH and LH, which employ tissue receptors from rat testis and highly purified human FSH (LER 1575-C) and LH (Hartree IRC-2, 24/6/69) as standards, have been developed to determine the FSH-like and LH-like activities in pregnant mare serum gonadotrophin (PMSG). Measurements of FSH and LH concentrations in the serum of six pregnant Pony mares showed that the ratio of these two activities did not vary significantly between mares and remained constant between days 40 and 80 of gestation with a value of 1·45 ± 0·04 (s.e.m.). The FSH:LH ratio of PMSG produced by cultured equine trophoblast cells was found to be 0·72 ± 0·03 (s.e.m.) and that of partially purified serum extracts of PMSG 1·08 (range 0·87–1·30).

GONADOTROPHIN REQUIREMENTS FOR IMPLANTATION IN THE MOUSE

1971 ◽  
Vol 50 (1) ◽  
pp. 19-27 ◽  
Author(s):  
B. M. BINDON
Keyword(s):  
Luteinizing Hormone ◽  
Half Life ◽  
Single Injection ◽  
Follicle Stimulating Hormone ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Suckling Mice ◽  
Rat Pituitary ◽  
Pituitary Glands ◽  
Pregnant Mare Serum Gonadotrophin

SUMMARY Gonadotrophins were injected into mated hypophysectomized and suckling mice in an attempt to induce implantation. In these two classes of animal implantation is normally delayed by absence or suppression of pituitary gonadotrophin release. Antibodies raised against ovine gonadotrophins were injected into mice soon after mating in an attempt to inhibit implantation. Pregnant mare serum gonadotrophin (PMSG) was effective in inducing implantation in both hypophysectomized and in suckling mice. This may mean that a gonadotrophin with the qualities of PMSG normally initiates implantation. Alternatively, PMSG may have been effective by virtue of its long half-life rather than any special hormonal attributes. Human chorionic gonadotrophin was ineffective in both types of mouse. Mixtures of ovine follicle-stimulating hormone (FSH) and luteinizing hormone (LH) (100 μg of each), injected daily for 3 days, were necessary to induce implantation in hypophysectomized mice. Implantation was readily induced in suckling mice by a single injection of FSH (equivalent to 12·5 μg NIH-FSH-S3) prepared from rat pituitary glands. Implantation was readily inhibited by anti-ovine LH. Anti-ovine FSH was ineffective but this did not cross-react with mouse FSH.

Luteinizing hormone and follicle stimulating hormone induce premature condensation of chromatin in goat (Capra hircus) oocytes

1991 ◽  
Vol 3 (5) ◽  
pp. 585 ◽  
Author(s):  
J Kumar ◽  
JC Osborn ◽  
AW Cameron
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Germinal Vesicle ◽  
Capra Hircus ◽  
High Dose ◽  
Germinal Vesicle Breakdown ◽  
Nuclear Maturation ◽  
Plasma Injection ◽  
High Doses ◽  
Stimulating Hormone

This study tested the hypothesis that premature condensation of chromatin in goat oocytes following superovulation with 1200 i.u. pregnant mare serum gonadotrophin (PMSG) is mediated by the high luteinizing hormone (LH) activity inherent in this gonadotrophin. Goats were treated with either a standard (3.95 mL) or high (7.90 mL) dose of a highly purified follicle-stimulating hormone (FSH) preparation (Ovagen), and different doses of human chorionic gonadotrophin (hCG) were added to increase the level of LH bioactivity during superovulation. The meiotic status of oocytes obtained at sponge withdrawal was compared between different treatments and correlated with profiles of LH bioactivity in peripheral plasma. Injection of 100 i.u. hCG (which gave a plasma LH profile comparable to 1200 i.u. PMSG) or 200 i.u. hCG resulted in significantly more oocytes showing premature condensation of chromatin without germinal vesicle breakdown than with 25 i.u. hCG or treatment with FSH alone. Nevertheless, nuclear maturation was also prematurely activated in a significant number of oocytes with a high dose of FSH alone, even though LH bioactivity was not detected in plasma. It is concluded that high LH bioactivity during superovulation of goats with gonadotrophins activates the initial stages of nuclear maturation in oocytes. However, highly purified FSH preparations in high doses can also induce this apparent abnormality in the timing of oocyte maturation through mechanisms unrelated to any LH contamination.

ADMINISTRATION OF ANTISERUM AGAINST OVINE FOLLICLE-STIMULATING HORMONE OR OVINE LUTEINIZING HORMONE AT PRO-OESTRUS IN THE RAT: EFFECTS ON FOLLICULAR DEVELOPMENT DURING THE ONCOMING CYCLE

1976 ◽  
Vol 70 (2) ◽  
pp. 301-306 ◽  
Author(s):  
R. WELSCHEN ◽  
J. DULLAART
Keyword(s):  
Luteinizing Hormone ◽  
Histological Study ◽  
Follicle Stimulating Hormone ◽  
Follicular Development ◽  
Antral Follicles ◽  
Lh Release ◽  
Ovulatory Follicles ◽  
Pregnant Mare Serum Gonadotrophin ◽  
Stimulating Hormone

SUMMARY Cyclic rats received at 13.00 h on the day of pro-oestrus a single i.v. injection of one of the following antiserum preparations: AOLH (raised in rabbits against NIH-LH-S17); AOFSH (raised against NIH-FSH-S9) or pAOFSH (AOFSH preincubated with 195 μg NIH-LH-S16/ml). Rats were killed at day 1, 3 or 5 after injection, and the ovaries prepared for histological study of the antral follicles. After AOLH, ovulation and resumption of meiosis in oocytes in pre-ovulatory follicles were prevented but follicular development during the following cycle appeared undisturbed. After either AOFSH or pAOFSH, blockade of ovulation was never observed but the formation of antral follicles normally occurring between mid-pro-oestrus and mid-oestrus was postponed by about one day. The later development of antral follicles might reflect a supranormal compensatory secretion of endogenous gonadotrophin because the development does not occur in AOFSH- or pAOFSH-treated rats hypophysectomized 24 h after injection and subsequently treated with pregnant mare serum gonadotrophin in dosage approximating the amount of gonadotrophin secreted endogenously during dioestrus. The results imply (1) that the pre-ovulatory surge of LH release is not essential for follicular development during the oncoming cycle whereas (2) a surge of FSH release is required for the formation of the new cohort of antral follicles that is normally seen at the start of a new cycle.

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