Change in distribution of cytoskeleton-associated proteins, lasp-1 and palladin, during uterine receptivity in the rat endometrium

2018 ◽  
Vol 30 (11) ◽  
pp. 1482 ◽  
Author(s):  
Leigh Nicholson ◽  
Laura Lindsay ◽  
Christopher R. Murphy
Keyword(s):  
Plasma Membrane ◽  
Focal Adhesion ◽  
Early Pregnancy ◽  
Morphological Changes ◽  
Cytoskeletal Proteins ◽  
Initial Contact ◽  
Luminal Epithelium ◽  
Uterine Receptivity ◽  
Uterine Lumen ◽  
Associated Proteins

The epithelium of the uterine lumen is the first point of contact with the blastocyst before implantation. To facilitate pregnancy, these uterine epithelial cells (UECs) undergo morphological changes specific to the receptive uterus. These changes include basal, lateral and apical alterations in the plasma membrane of UECs. This study looked at the cytoskeletal and focal adhesion-associated proteins, lasp-1 and palladin, in the uterus during early pregnancy in the rat. Two palladin isoforms, 140 kDa and 90 kDa, were analysed, with the migration-associated 140-kDa isoform increasing significantly at the time of implantation when compared with the time of fertilisation. Lasp-1 was similarly increased at this time, whilst also being located predominantly apically and laterally in the UECs, suggesting a role in the initial contact between the UECs and the blastocyst. This is the first study to investigate palladin and lasp-1 in the uterine luminal epithelium and suggests an importance for these cytoskeletal proteins in the morphological changes the UECs undergo for pregnancy to occur.

scholarly journals Structural Determinants and Their Role in Cyanobacterial Morphogenesis

Life ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 355
Author(s):  
Benjamin L. Springstein ◽  
Dennis J. Nürnberg ◽  
Gregor L. Weiss ◽  
Martin Pilhofer ◽  
Karina Stucken
Keyword(s):  
Cell Division ◽  
Cell Growth ◽  
Morphological Changes ◽  
Coiled Coil ◽  
Cytoskeletal Proteins ◽  
Specific Cell ◽  
Growth Strategies ◽  
Structural Determinants ◽  
Gram Negative ◽  
Associated Proteins

Cells have to erect and sustain an organized and dynamically adaptable structure for an efficient mode of operation that allows drastic morphological changes during cell growth and cell division. These manifold tasks are complied by the so-called cytoskeleton and its associated proteins. In bacteria, FtsZ and MreB, the bacterial homologs to tubulin and actin, respectively, as well as coiled-coil-rich proteins of intermediate filament (IF)-like function to fulfil these tasks. Despite generally being characterized as Gram-negative, cyanobacteria have a remarkably thick peptidoglycan layer and possess Gram-positive-specific cell division proteins such as SepF and DivIVA-like proteins, besides Gram-negative and cyanobacterial-specific cell division proteins like MinE, SepI, ZipN (Ftn2) and ZipS (Ftn6). The diversity of cellular morphologies and cell growth strategies in cyanobacteria could therefore be the result of additional unidentified structural determinants such as cytoskeletal proteins. In this article, we review the current advances in the understanding of the cyanobacterial cell shape, cell division and cell growth.

scholarly journals Signal transducer and activator of transcription 3 (Stat3) expression and activation in rat uterus during early pregnancy

Reproduction ◽  
2004 ◽  
Vol 128 (2) ◽  
pp. 197-205 ◽  
Author(s):  
Chun-Bo Teng ◽  
Hong-Lu Diao ◽  
Hong Ma ◽  
Jing Cong ◽  
Hao Yu ◽  
...  
Keyword(s):  
Early Pregnancy ◽  
Stromal Cells ◽  
Embryo Implantation ◽  
Luminal Epithelium ◽  
Signal Transducer ◽  
Uterine Receptivity ◽  
Rat Uterus ◽  
Stat3 Phosphorylation ◽  
Decidual Cells ◽  
Transcription 3

Signal transducer and activator of transcription 3 (Stat3), a member of the Stat family, is specifically activated during mouse embryo implantation. The aim of this study was to investigate the expression, activation and regulation of Stat3 in rat uterus during early pregnancy, pseudopregnancy, delayed implantation and artificial decidualization. Stat3 mRNA was highly expressed in the luminal epithelium on day 5 and in the luminal epithelium and underlying stromal cells at implantation sites on day 6 of pregnancy. There was a strong level of Stat3 protein expression and phosphorylation in the stromal cells near the lumen and in the luminal epithelium on day 5 of pregnancy, which was similar to day 5 of pseudopregnancy. In the afternoon of day 6, the strong level of Stat3 phosphorylation was detected only in the luminal epithelium. Stat3 was highly expressed and activated in the decidual cells from days 7 to 9 of pregnancy and under artificial decidualization in the present study. Our results suggest that the strong level of Stat3 activation in the luminal epithelium and underlying stromal cells during the pre-implantation period may be important for establishing uterine receptivity as in mice, and the high level of Stat3 expression and activation in decidual cells may play a role during decidualization.

THE PLASMA MEMBRANE TRANSFORMATION: A KEY CONCEPT IN UTERINE RECEPTIVITY

2001 ◽  
Vol 9 (3) ◽  
pp. 197-208 ◽  
Author(s):  
CR Murphy
Keyword(s):  
Plasma Membrane ◽  
Epithelial Cells ◽  
Early Pregnancy ◽  
Fetal Tissue ◽  
Human Beings ◽  
Human Uterus ◽  
Uterine Receptivity ◽  
Uterine Epithelial Cells ◽  
Considerable Body ◽  
Membrane Transformation

The first site of contact between maternal and fetal tissue at the beginning of blastocyst attachment and implantation is the plasma membrane of uterine epithelial cells. Indeed, as has been noted often, regardless of the mode of placentation which ultimately occurs, contact between this plasma membrane and that of the trophoblast is a common beginning to implantation in most species studied so far, which now range from viviparous lizards to human beings. The similarities in these early events of uterine receptivity and placentation go further than mere contact between opposing surfaces however. A considerable body of evidence indicates that the behaviour of the plasma membrane of uterine epithelial cells during early pregnancy has many common aspects across species including humans. This review pays special attention to events in the human uterus and the epithelial cells in particular, but examines them within the wider context of uterine receptivity for implantation across species.

238. Claudins and occludin in the rat endometrium

2005 ◽  
Vol 17 (9) ◽  
pp. 94
Author(s):  
M. D. O. Nicholson ◽  
C. R. Murphy
Keyword(s):  
Epithelial Cells ◽  
Tight Junctions ◽  
Early Pregnancy ◽  
Immunofluorescence Microscopy ◽  
Ovarian Hormones ◽  
Cellular Distribution ◽  
Luminal Epithelium ◽  
Uterine Lumen ◽  
Uterine Glands ◽  
Ovariectomised Rats

Regulation of the uterine luminal environment is important for the successful attachment and implantation of the blastocyst. Tight junctions regulate the paracellular pathway between epithelial cells lining the uterine lumen and the uterine glands. The aims of this present study was firstly to establish the presence and cellular distribution of claudins and occludin in the luminal epithelia during early pregnancy using immunofluorescence microscopy and deconvolution, and secondly to determine the influence of ovarian hormones on their expression. Occludin and claudins -1, -3, and -4 were present in luminal epithelium. Occludin and claudin-4 showed increased expression in luminal epithelium at the time of implantation, whereas claudin-1 and -3 expression remained the same throughout early pregnancy. In ovariectomised rats administered ovarian hormones, occludin and claudin-4 showed increased expression in luminal epithelium in progesterone-dominant regimes and decreased expression when administered oestrogen alone. Expression of claudin-1 and -3 in luminal epithelium was not effected by ovarian hormones. Claudin-2 was not expressed during early pregnancy nor in ovariectomised rats. In conclusion, these results show that occludin and claudins -1, -3 and -4 are present in luminal and glandular epithelium, and provide the permeability properties needed to separate the luminal and the stromal environment at the time of implantation. Furthermore, occludin and claudin-4 expression is controlled by ovarian hormones being upregulated by progesterone.

237. Aquaporins in rat uterine epithelial cells during early pregnancy and in response to progesterone

2005 ◽  
Vol 17 (9) ◽  
pp. 93
Author(s):  
L. A. Lindsay ◽  
C. R. Murphy
Keyword(s):  
Plasma Membrane ◽  
Epithelial Cells ◽  
Early Pregnancy ◽  
Water Movement ◽  
Immunogold Electron Microscopy ◽  
Apical Plasma Membrane ◽  
Uterine Lumen ◽  
Epithelial Barriers ◽  
Luminal Epithelial Cells ◽  
Ovariectomised Rats

Implantation of the rat blastocyst is a highly regulated process, involving transformation of the uterine environment into one which is receptive to an implanting blastocyst. At the time of implantation, in response to progesterone, there is a dramatic decrease in the amount of uterine luminal fluid leading to close apposition between the luminal epithelium and trophoblastic cells. The rat blastocyst also always implants at the antimesometrial pole of the uterine lumen and currently mechanisms regulating this process are unknown. Aquaporins, a family of transmembrane water channels, are involved in the regulation of water movement across epithelial barriers. We investigated several aquaporins in the rat uterus during early pregnancy using reverse transcriptase PCR. Immunofluorescence and immunogold electron microscopy techniques were then used to investigate the localisation of particular aquaporins including AQP5 in the uterine epithelium during early pregnancy and in ovariectomised rats treated with progesterone. There was an increase in AQP5 molecules in the apical plasma membrane of luminal epithelial cells at the time of implantation, with a greater increase at the mesometrial compared to antimesometrial pole. A similar result was seen in luminal epithelial cells from ovariectomised rats treated with progesterone, however there was no differential concentration between mesometrial and antimesometrial poles, as there was during early pregnancy. It is suggested that the increase in AQP5 protein expression in the apical plasma membrane of luminal epithelial cells is involved in reabsorption of luminal fluid at the time of implantation. Furthermore, the differential concentration of AQP5 on luminal epithelial cells at the time of implantation could lead to the establishment of a fluid gradient within the uterine lumen and hence lead to the asymmetrical implantation position of the rat blastocyst.

scholarly journals The actin cytoskeleton and plasma membrane connection: PtdIns(4,5)P(2) influences cytoskeletal protein activity at the plasma membrane

2000 ◽  
Vol 113 (21) ◽  
pp. 3685-3695 ◽  
Author(s):  
A.S. Sechi ◽  
J. Wehland
Keyword(s):  
Plasma Membrane ◽  
Actin Cytoskeleton ◽  
Adhesion Molecules ◽  
Focal Adhesion ◽  
Small Gtpases ◽  
Cytoskeletal Protein ◽  
Protein Activity ◽  
Associated Proteins ◽  
Tight Connection

The co-ordination of rearrangements of the actin cytoskeleton depends on its tight connection to the plasma membrane. Phosphatidylinositol 4,5-bisphosphate is thought to transmit signals originating at the plasma membrane to the underlying actin cytoskeleton. This lipid binds to, and influences the activity of, several actin-associated proteins in vitro that regulate the architecture of the actin cytoskeleton. Signalling intermediates in this process include focal adhesion molecules such as vinculin and members of two families of proteins, ERM and WASP. These proteins interact with phosphatidylinositol 4,5-bisphosphate and appear to be regulated by interplay between small GTPases and phosphatidylinositol 4,5-bisphosphate metabolism, and thus link the plasma membrane with cytoskeletal remodelling.

scholarly journals Binding of microtubule-associated protein 1B to LIS1 affects the interaction between dynein and LIS1

2005 ◽  
Vol 389 (2) ◽  
pp. 333-341 ◽  
Author(s):  
Eva M. JiméNez-Mateos ◽  
Francisco Wandosell ◽  
Orly Reiner ◽  
Jesús Avila ◽  
Christian González-Billault
Keyword(s):  
Neuronal Migration ◽  
Hippocampal Neurons ◽  
Morphological Changes ◽  
Molecular Motor ◽  
Cytoskeletal Proteins ◽  
Actin Binding ◽  
Microtubule Associated Proteins ◽  
Neural Processes ◽  
Associated Proteins ◽  
Differential Binding

For neuronal migration to occur, the cell must undergo morphological changes that require modifications of the cytoskeleton. Several different MAPs (microtubule-associated proteins) or actin-binding proteins are proposed to be involved in the migration of neurons. Therefore we have specifically analysed how two members of the MAP family, MAP1B and LIS1 (lissencephaly-related protein 1), interact with one another and participate in neuronal migration. Our results indicate that, in hippocampal neurons, MAP1B and LIS1 co-localize, associate and interact with each another. The interaction between these two MAPs is regulated by the phosphorylation of MAP1B. Furthermore, this interaction interferes with the association between LIS1 and the microtubule-dependent molecular motor, dynein. Clearly, the differential binding of these cytoskeletal proteins could regulate the functions attributed to the LIS1–dynein complex, including those related to extension of the neural processes necessary for neuronal migration.

THE EFFECTS OF AN INTRA-UTERINE DEVICE ON UTERINE CELL DIVISION AND EPITHELIAL MORPHOLOGY DURING EARLY PREGNANCY IN THE MOUSE

1970 ◽  
Vol 48 (3) ◽  
pp. 347-NP ◽  
Author(s):  
L. MARTIN ◽  
C. A. FINN
Keyword(s):  
Cell Division ◽  
Early Pregnancy ◽  
Luminal Epithelium ◽  
Mitogenic Effect ◽  
Oestrogen Treatment ◽  
Uterine Lumen ◽  
Epithelial Morphology ◽  
Suppressive Action

SUMMARY Intra-uterine silk threads in mice prevented the development of a normal progestational epithelium and closure of the uterine lumen on day 5 of pregnancy. It seems likely that either of these effects would prevent implantation. The intra-uterine device (IUD) produced an abnormal type of luminal epithelium resembling somewhat the hyperplastic type produced by oestrogen treatment, and characterized by increased cytoplasmic basophilia and irregularly placed, swollen nuclei with prominent nucleoli. The IUD also increased the numbers of mitoses in the luminal epithelium. This mitogenic effect was not due simply to inhibition of the suppressive action of progesterone since mitosis was increased on day 3 when progesterone is probably not involved. All these effects were seen in regions of the uterus remote from the site of the IUD, which suggests that they are caused by factors released into the uterine lumen. In contrast, the effects of the IUD on the glands and stroma appeared to be restricted to the immediate vicinity of the IUD. It is suggested that IUD's might prevent implantation by stimulating the production of substances which antagonize the actions of progesterone on the uterine luminal epithelium.

Biotinylation and Purification of Plasma Membrane-associated Proteins from Rodent Cultured Neurons

BIO-PROTOCOL ◽  
2016 ◽  
Vol 6 (10) ◽  
Author(s):  
Margarida Caldeira ◽  
Joana Ferreira ◽  
Ana Carvalho ◽  
Carlos Duarte
Keyword(s):  
Plasma Membrane ◽  
Cultured Neurons ◽  
Associated Proteins
Sign in / Sign up

Export Citation Format

Share Document