Anabolic effects of chrysin on the ventral male prostate and female prostate of adult gerbils (Meriones unguiculatus)

2018 ◽  
Vol 30 (9) ◽  
pp. 1180 ◽  
Author(s):  
Mônica S. Campos ◽  
Naiara C. S. Ribeiro ◽  
Rodrigo F. de Lima ◽  
Mariana B. Santos ◽  
Patrícia S. L. Vilamaior ◽  
...  
Keyword(s):  
Secretory Pathway ◽  
Intraepithelial Neoplasia ◽  
Meriones Unguiculatus ◽  
Epithelial Hyperplasia ◽  
Female Adult ◽  
Experimental Conditions ◽  
Cellular Toxicity ◽  
Male And Female ◽  
Female Prostate ◽  
Testicular Morphology

Chrysin is a bioflavonoid found in fruits, flowers, tea, honey and wine, which has antioxidant, anti-inflammatory, antiallergic and anticarcinogenic properties. This flavone has also been considered as beneficial for reproduction due its testosterone-boosting potential. Thus, the aim of this study was to evaluate the effects of chrysin on the prostate and gonads of male and female adult gerbils. In addition, a comparative analysis of the effects of testosterone on these same organs was conducted. Ninety-day-old male and female gerbils were treated with chrysin (50 mg kg−1 day−1) or testosterone cypionate (1 mg kg−1 week−1) for 21 days. The ventral male prostate and female prostate were dissected out for morphological, morphometric–stereological and ultrastructural assays. Testes and ovaries were submitted to morphological and morphometric­­–stereological analyses. Chrysin treatment caused epithelial hyperplasia and stromal remodelling of the ventral male and female prostate. Ultrastructurally, male and female prostatic epithelial cells in the chrysin group presented marked development of the organelles involved in the biosynthetic–secretory pathway, whereas cellular toxicity was observed only in female glands. Chrysin preserved normal testicular morphology and increased the number of growing ovarian follicles. Comparatively, testosterone treatment was detrimental to the prostate and gonads, since foci of prostatic intraepithelial neoplasia and gonadal degeneration were observed in both sexes. Thus, under the experimental conditions of this study, chrysin was better tolerated than testosterone in the prostate and gonads.

Combined oral contraceptives promote androgen receptor and oestrogen receptor alpha upregulation in the female prostate (Skene’s paraurethral glands) of adult gerbils (Meriones unguiculatus)

2018 ◽  
Vol 30 (10) ◽  
pp. 1286
Author(s):  
Eliana G. Pinto ◽  
Mônica S. Campos ◽  
Luiz R. Falleiros-Júnior ◽  
Mara R. Marques ◽  
Sebastião R. Taboga ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Hormonal Regulation ◽  
Oestrogen Receptor ◽  
Combined Treatment ◽  
Secretory Activity ◽  
Intraepithelial Neoplasia ◽  
Meriones Unguiculatus ◽  
Proliferation Index ◽  
Cell Degeneration ◽  
Female Prostate

The aim of this study was to evaluate the effects of cyproterone acetate (CPA) and ethinyloestradiol (EE) alone or in combination on the female prostate of adult gerbils. Adult females were exposed for 21 days to daily oral doses of CPA (1 mg kg−1), EE (10 µg kg−1) or a combination of CPA and EE. Female prostatic complexes were removed, weighed and subjected to morphological, stereological, immunohistochemical and ultrastructural analyses. CPA treatment caused epithelial atrophy and decreased prostate secretory activity. The EE treatment group showed glandular hyperplasia, a high cell-proliferation index and an increase in androgen and oestrogen receptor α (AR and ERα) immunoreactivity. Combined treatment (CPA+EE) caused adverse effects, such as an increase in cell proliferation, higher AR and ERα immunoreactivity, prostatic intraepithelial neoplasia, cell degeneration and aging. In conclusion, the CPA-only treatment promoted antiandrogenic effects on the female gerbil prostate, whereas EE-only had a potent oestrogenic activity. However, when combined, EE overlapped the effects of CPA, changing the pattern of glandular hormonal regulation and stimulating the development of prostatic lesions in female gerbils.

THE PITUITARY AND ADRENAL RESPONSES TO ADMINISTRATION OF OESTRIOL IN GONADECTOMIZED RATS

1961 ◽  
Vol 38 (1) ◽  
pp. 50-58 ◽  
Author(s):  
N. E. Borglin ◽  
L. Bjersing
Keyword(s):  
Pituitary Gland ◽  
Adrenal Cortex ◽  
Clinical Experience ◽  
Uterine Cervix ◽  
Morphological Changes ◽  
Physiological Significance ◽  
Female Rats ◽  
Experimental Conditions ◽  
Male And Female ◽  
Male And Female Rats

ABSTRACT Oestriol (oestra-1,3,5(10)-triene-3,16α,17β-triol) is a weakly oestrogenic substance which, however, in contrast to what was formerly believed, is of physiological significance. Its effect is localized largely to the uterine cervix and vagina. Clinical experience argues both for and against an effect on the pituitary gland. This investigation is concerned with the morphological changes in the pituitary gland and adrenal cortex of gonadectomized male and female rats after the injection of oestriol. It was found that oestriol has the same type of action on these glands as other oestrogens, but under the experimental conditions used, this effect proved much weaker than that produced by oestradiol (oestra-1,3,5(10)-triene-3,17β-diol).

The impact of maternal protein restriction during perinatal life on the response to a septic insult in adult rats

2020 ◽  
pp. 1-8
Author(s):  
Reza Khazaee ◽  
Anastasiya Vinokurtseva ◽  
Lynda A. McCaig ◽  
Cory Yamashita ◽  
Daniel B. Hardy ◽  
...  
Keyword(s):  
Control Diet ◽  
Protein Restriction ◽  
Saline Control ◽  
Female Adult ◽  
Adult Rats ◽  
Male And Female ◽  
Maternal Protein Restriction ◽  
And Control ◽  
The Impact ◽  
Septic Insult

Abstract Although abundant evidence exists that adverse events during pregnancy lead to chronic conditions, there is limited information on the impact of acute insults such as sepsis. This study tested the hypothesis that impaired fetal development leads to altered organ responses to a septic insult in both male and female adult offspring. Fetal growth restricted (FGR) rats were generated using a maternal protein-restricted diet. Male and female FGR and control diet rats were housed until 150–160 d of age when they were exposed either a saline (control) or a fecal slurry intraperitoneal (Sepsis) injection. After 6 h, livers and lungs were analyzed for inflammation and, additionally, the amounts and function of pulmonary surfactant were measured. The results showed increases in the steady-state mRNA levels of inflammatory cytokines in the liver in response to the septic insult in both males and females; these responses were not different between FGR and control diet groups. In the lungs, cytokines were not detectable in any of the experimental groups. A significant decrease in the relative amount of surfactant was observed in male FGR offspring, but this was not observed in control males or in female animals. Overall, it is concluded that FGR induced by maternal protein restriction does not impact liver and lung inflammatory response to sepsis in either male or female adult rats. An altered septic response in male FGR offspring with respect to surfactant may imply a contribution to lung dysfunction.

scholarly journals Aortic depressor nerve morphometry in male and female adult spontaneously hypertensive rats.

2013 ◽  
Vol 27 (S1) ◽  
Author(s):  
Milena Menezes Amorim ◽  
Jaci Airton Castania ◽  
Helio Cesar Salgado ◽  
Valéria Paula Sassoli Fazan
Keyword(s):  
Spontaneously Hypertensive Rats ◽  
Hypertensive Rats ◽  
Female Adult ◽  
Male And Female ◽  
Spontaneously Hypertensive ◽  
Aortic Depressor Nerve ◽  
Nerve Morphometry

scholarly journals Effects of Sex and 17 β-Estradiol on Cardiac Fibroblast Morphology and Signaling Activities In Vitro

Cells ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2564
Author(s):  
Kelsey Watts ◽  
William J. Richardson
Keyword(s):  
Cardiac Fibrosis ◽  
Cardiac Fibroblasts ◽  
Cellular Level ◽  
Cellular Morphology ◽  
Structural Level ◽  
Experimental Conditions ◽  
Male And Female ◽  
Protein Protein Interaction ◽  
Complex Signaling

Several studies have demonstrated estrogen’s cardioprotective abilities in decreasing the fibrotic response of cardiac fibroblasts (CFs). However, the majority of these studies are not sex-specific, and those at the cellular level utilize tissue culture plastic, a substrate with a much higher stiffness than physiological conditions. Understanding the intrinsic differences between male and female CFs under more physiologically “healthy” conditions will help to elucidate the divergences in their complex signaling networks. We aimed to do this by conducting a sex-disaggregated analysis of changes in cellular morphology and relative levels of profibrotic signaling proteins in CFs cultured on 8 kPa stiffness plates with and without 17 β-estradiol (E2). Cyclic immunofluorescent analysis indicated that there was a negligible change in cellular morphology due to sex and E2 treatment and that the differences between male and female CFs occur at a biochemical rather than structural level. Several proteins corresponding to profibrotic activity had various sex-specific responses with and without E2 treatment. Single-cell correlation analysis exhibited varied protein–protein interaction across experimental conditions. These findings demonstrate the need for further research into the dimorphisms of male and female CFs to develop better tailored sex-informed prevention and treatment interventions of cardiac fibrosis.

scholarly journals Subchronic Toxicity Assessment of Phytolacca americana L. (Phytolaccaceae) in F344 Rats

2020 ◽  
Vol 15 (7) ◽  
pp. 1934578X2094165
Author(s):  
Hyoung-Yun Han ◽  
Kang-Hyun Han ◽  
Jun-Ho Ahn ◽  
Se-Myo Park ◽  
Soojin Kim ◽  
...  
Keyword(s):  
Body Weight ◽  
Adverse Effects ◽  
Clinical Chemistry ◽  
Toxicity Assessment ◽  
Phytolacca Americana ◽  
Subchronic Toxicity ◽  
Experimental Conditions ◽  
Male And Female ◽  
Long Term Treatment ◽  
F344 Rats

Phytolacca americana L. is traditionally used in Korea, Japan, and China as a diuretic, antibacterial, antiviral, anticancer, and anti-inflammatory agent, and also in the treatment of hepatitis B, psoriasis, edema, and rheumatism. In this study, we evaluated the subchronic toxicity of an aqueous extract of P. americana (PAAE) in male and female F344 rats. The rats were orally administered PAAE (0, 500, 1000, and 2000 mg/kg body weight) once daily for 13 weeks. Mortality rate, body weight, food consumption, and organ weights were measured and assessed. Additionally, ophthalmological, hematological, and histopathological parameters were evaluated. Urinalysis and necropsy were also performed. The clinical chemistry values for potassium in the treated female groups (500, 1000, and 2000 mg/kg/ body weight/day) were higher than those in the control. Further, the relative weights of the kidneys in the treated female groups (1000 and 2000 mg/kg/ body weight/day) were higher than those in the control. However, these changes were not consistent in either sex, and no abnormalities were found in the corresponding pathological findings. Thus the results showed no adverse effects in all the parameters assessed. The findings show that after 13 weeks of treatment, the “no-observed-adverse-effect level” of PAAE is 2000 mg/kg body weight in both male and female F344 rats under the experimental conditions applied. Although treatment-related adverse effects were not seen, potassium-level changes in the blood should be examined to establish the safety profile of PAAE after long-term treatment.

258 Comparing Post-hospitalization Global Health Scale Assessments in Male and Female Adult Burn Patients for Quality of Life

2018 ◽  
Vol 39 (suppl_1) ◽  
pp. S96-S96
Author(s):  
N Alver ◽  
K Koetsier ◽  
G Carrougher ◽  
L Muffley ◽  
N Gibran
Keyword(s):  
Quality Of Life ◽  
Global Health ◽  
Burn Patients ◽  
Female Adult ◽  
Male And Female
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