scholarly journals Anti-Müllerian hormone receptor type 2 is expressed in gonadotrophs of postpubertal heifers to control gonadotrophin secretion

2018 ◽  
Vol 30 (9) ◽  
pp. 1192 ◽  
Author(s):  
Onalenna Kereilwe ◽  
Kiran Pandey ◽  
Vitaliano Borromeo ◽  
Hiroya Kadokawa
Keyword(s):  
Immunofluorescence Microscopy ◽  
Receptor Type ◽  
Chain Reaction ◽  
Extracellular Region ◽  
Gonadotrophin Secretion ◽  
Secretion Expression ◽  
Gonadotrophin Releasing Hormone ◽  
Polymerase Chain ◽  
Lh Secretion

Preantral and small antral follicles may secret anti-Müllerian hormone (AMH) to control gonadotrophin secretion from ruminant gonadotrophs. The present study investigated whether the main receptor for AMH, AMH receptor type 2 (AMHR2), is expressed in gonadotrophs of postpubertal heifers to control gonadotrophin secretion. Expression of AMHR2 mRNA was detected in anterior pituitaries (APs) of postpubertal heifers using reverse transcription–polymerase chain reaction. An anti-AMHR2 chicken antibody was developed against the extracellular region near the N-terminus of bovine AMHR2. Western blotting using this antibody detected the expression of AMHR2 protein in APs. Immunofluorescence microscopy using the same antibody visualised colocalisation of AMHR2 with gonadotrophin-releasing hormone (GnRH) receptor on the plasma membrane of gonadotrophs. AP cells were cultured for 3.5 days and then treated with increasing concentrations (0, 1, 10, 100, or 1000 pg mL−1) of AMH. AMH (10–1000 pg mL−1) stimulated (P < 0.05) basal FSH secretion. In addition, AMH (100–1000 pg mL−1) weakly stimulated (P < 0.05) basal LH secretion. AMH (100–1000 pg mL−1) inhibited GnRH-induced FSH secretion, but not GnRH-induced LH secretion, in AP cells. In conclusion, AMHR2 is expressed in gonadotrophs of postpubertal heifers to control gonadotrophin secretion.

scholarly journals Anti-Müllerian hormone receptor type 2 (AMHR2) expression in bovine oviducts and endometria: comparison of AMHR2 mRNA and protein abundance between old Holstein and young and old Wagyu females

2020 ◽  
Vol 32 (8) ◽  
pp. 738
Author(s):  
Raihana Nasrin Ferdousy ◽  
Onalenna Kereilwe ◽  
Hiroya Kadokawa
Keyword(s):  
Polymerase Chain Reaction ◽  
Protein Expression ◽  
Receptor Type ◽  
Protein Abundance ◽  
Chain Reaction ◽  
Antral Follicles ◽  
Gonadotrophin Secretion ◽  
Mrna And Protein Expression ◽  
Polymerase Chain

Anti-Müllerian hormone (AMH) is a glycoprotein produced by granulosa cells of preantral and small antral follicles that has multiple important roles in the ovaries. Recent studies have revealed extragonadal AMH regulation of gonadotrophin secretion from bovine gonadotrophs. In this study we investigated whether the primary receptor for AMH, AMH receptor type 2 (AMHR2), is expressed in bovine oviducts and endometria. Reverse transcription–polymerase chain reaction detected expression of AMHR2 mRNA in oviductal and endometrial specimens. Western blotting and immunohistochemistry were performed to analyse AMHR2 protein expression using anti-bovine AMHR2 antibody. Immunohistochemistry revealed robust AMHR2 expression in the tunica mucosa of the ampulla and isthmus, as well as in the glandular and luminal epithelium of the endometrium. AMHR2 mRNA (measured by real-time polymerase chain reaction) and AMHR2 protein expression in these layers did not significantly differ among oestrous phases in adult Wagyu cows (P&gt;0.1). In addition, AMHR2 mRNA and protein expression in these layers did not differ among old Holsteins (mean (±s.e.m.) age 91.9±6.4 months) and young (26.6±0.8 months) and old (98.8±10.2 months) Wagyu cows. Therefore, AMHR2 is expressed in bovine oviducts and endometria.

Molecular characterisation of ovine herpesvirus type 2 (OvHV-2) in Turkey

2012 ◽  
Vol 60 (4) ◽  
pp. 521-527 ◽  
Author(s):  
Yakup Yildirim ◽  
Seval Bilge Dağalp ◽  
Volkan Yilmaz ◽  
Ali Faraji Majarashin
Keyword(s):  
Clinical Signs ◽  
Peripheral Blood Leukocyte ◽  
Malignant Catarrhal Fever ◽  
Molecular Characterisation ◽  
Laboratory Examination ◽  
Chain Reaction ◽  
Herpesvirus Type ◽  
Polymerase Chain ◽  
Blood Leukocyte

In this study, the physical examination of 22 cattle revealed clinical signs of malignant catarrhal fever (MCF). Peripheral blood leukocyte (PBL) samples of the 22 cattle, and nasal (n = 7) and conjunctival (n = 9) swab samples from 16 sheep from two different farms, were taken for laboratory examination. The clinical diagnosis of MCF in cows was confirmed by the detection of ovine herpesvirus type 2 (OvHV-2) DNA by polymerase chain reaction (PCR). OvHV-2 DNA was detected by nested-PCR in PBL of one cow with clinical signs and nasal (1/7)-conjunctival(1/9) swab samples of two sheep housed in the same barn. According to the sequence analysis, three slightly divergent viruses were detected. The results indicate the need for additional research in different regions of Turkey to gain a better understanding of the incidence of MCF and its implications for the livestock industry.

scholarly journals Vaginal and Extra-Vaginal Bacterial Colonization and Risk for Incident Bacterial Vaginosis in a Population of Women Who Have Sex With Men

2020 ◽  
Author(s):  
David N Fredricks ◽  
Anna Plantinga ◽  
Sujatha Srinivasan ◽  
Antoinette Oot ◽  
Andrew Wiser ◽  
...  
Keyword(s):  
Bacterial Vaginosis ◽  
Vaginal Discharge ◽  
Bacterial Colonization ◽  
Quantitative Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Associated Bacteria ◽  
Vaginal Colonization ◽  
Polymerase Chain ◽  
Sex With Men

Abstract Background Bacterial vaginosis (BV) is a common cause of vaginal discharge and associated with vaginal acquisition of BV-associated bacteria (BVAB). Methods We used quantitative polymerase chain reaction assays to determine whether presence or concentrations of BVAB in the mouth, anus, vagina, or labia before BV predict risk of incident BV in 72 women who have sex with men. Results Baseline vaginal and extra-vaginal colonization with Gardnerella spp, Megasphaera spp, Sneathia spp, BVAB-2, Dialister sp type 2, and other BVAB was more common among subjects with incident BV. Conclusions Prior colonization with BVAB is a consistent risk for BV.

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