Prospective evaluation of uterine receptivity in mice

2018 ◽  
Vol 30 (4) ◽  
pp. 619 ◽  
Author(s):  
Hitomi Nakamura ◽  
Takayoshi Hosono ◽  
Keiichi Kumasawa ◽  
Tadashi Kimura
Keyword(s):  
Roc Curve ◽  
Implantation Failure ◽  
Uterine Receptivity ◽  
Roc Curve Analysis ◽  
Failure Group ◽  
Uterine Endometrium ◽  
Oxidation Reduction ◽  
Oxidation Reduction Potential ◽  
And Control

In current infertility treatments it is necessary to evaluate uterine receptivity in each menstrual cycle. During the implantation period, the uterus goes through many complex orchestrated changes, including changes to the glycocalyx. The changes to the glycocalyx are due to sialylation, sulfation and fucosylation. Can the measurement of in-vivo uterine pH and/or oxidation-reduction potential (ORP) determine the alterations of uterine endometrium for implantation and evaluate prospective uterine receptivity? In the present study we assessed in vivo uterine pH and ORP during the early stages of pregnancy in naïve mice, as well as in a murine model of implantation failure created by local and transient suppression of signal transducer and activator of transcription 3. There was no change in the in vivo uterine pH between post-coitus Days 2 and 6. In vivo uterine ORP was significantly higher compared to the day before. One day before implantation began, uterine ORP was significantly decreased in the implantation failure group compared with the naïve and control groups. Receiver operator characteristic (ROC) curve analysis of uterine ORP as a predictor of non-conception showed an area under the ROC curve of 0.96 (95% confidence interval 0.92–1.00). Thus, in vivo uterine ORP could be a parameter to prospectively evaluate uterine receptivity.

Apparatus and techniques for the measurement of oxidation-reduction potentials, pH and oxygen tension in vivo

1957 ◽  
Vol 146 (923) ◽  
pp. 289-297 ◽  
Keyword(s):  
Oxygen Tension ◽  
Reduction Potential ◽  
Model Experiments ◽  
Reduction Potentials ◽  
Oxidation Reduction ◽  
Oxidation Reduction Potential ◽  
Small Voltage

In order to follow the changes which occur in tumours during treatment with radiation, and the effects of radiosensitizing or radioprotecting drugs, apparatus and techniques have been developed to record automatically changes of oxidation-reduction potential on eight different electrodes. Simultaneous records of pH and oxygen tension changes were used as controls in some experiments. The changes of oxygen tension were followed by applying a known small voltage to one electrode and measuring the current which flowed. Calibration of oxygen-tension measurements was attempted by the use of model experiments.

scholarly journals Effects of Electromagnetic Fields on Colloidal Nano Silver for Applications in Nano Medicine

2020 ◽  
pp. 20-27
Author(s):  
Ignat Ignatov ◽  
Christos Drossinakis
Keyword(s):  
Energy Spectrum ◽  
Electromagnetic Fields ◽  
Electromagnetic Waves ◽  
Control Sample ◽  
Nano Silver ◽  
Oxidation Reduction ◽  
Oxidation Reduction Potential ◽  
Nano Medicine ◽  
And Control ◽  
Nonequilibrium Energy

Colloidal Silver in European Union is food additive. The aim of the study is to show the common effects of colloidal nano silver and electromagnetic fields. The influence on colloidal nano silver with concentration of 30 ppm was studied using the method of Drossinakis in electromagnetic waves in the range of ν=20 -70 Hz. The research was performed with the methods for spectral analyses Nonequilibrium energy spectrum (NES) and Differential nonequilibrium energy spectrum (DNES). The study was performed with research of parameters of pH and oxidation reduction potential (ORP). The control sample is the sample with colloidal nano silver. The sample was taken after the influence with electromagnetic fields on the sample with colloidal nano silver. The effect of electromagnetic fields is connected with increasing of the effects of colloidal nano silver.  There are proofs with differences between samples and control sample with parameters of NES, DNES, pH, ORP. 

Inhibitors of Tyrosinase

1955 ◽  
Vol 32 (3) ◽  
pp. 468-484
Author(s):  
M. G. M. PRYOR
Keyword(s):  
Methylene Blue ◽  
Dissolved Oxygen ◽  
Inhibitory Effect ◽  
Tyrosinase Activity ◽  
Aromatic Substrate ◽  
Oxidation Reduction ◽  
Oxidation Reduction Potential ◽  
Low Concentrations ◽  
Sulphydryl Groups

1. It has been reported that if Drosophila larvae are ground to a fine paste with sand, the homogenate shows little tyrosinase activity, but that if the larvae are allowed to blacken in chloroform vapour before grinding, activity is increased. 2. This has been interpreted as showing the effect of an intracellular inhibitor, set free by rupturing the cells, but destroyed by chloroform. This inhibitor has been identified by previous authors as a dehydrogenase. 3. It is here suggested that the lack of activity of Drosophila extracts prepared with sand is due to destruction of tyrosinase as it oxidizes naturally occurring aromatic substrates. 4. It is shown that tyrosinase is destroyed by oxidizing the aromatic substrate present in the cuticle of Calliphora larvae, or by very low concentrations of homocatechol. 5. The aromatic substrate of Calliphora larvae is concentrated in the cuticle, and would be set free by fine grinding. 6. Drosophila or Calliphora larvae yield a more active extract when ground with sand than when simply crushed, provided that they are tested soon after grinding. 7. The tyrosinase activity of such extracts is not increased by chloroform or methanol. 8. The compound between o-quinones and amino-acids is capable of oxidizing ascorbic acid or excess amino-acid without the aid of an enzyme, and of simultaneously reducing methylene blue. 9. This reaction, rather than the activity of dehydrogenases, is probably responsible for most of the ability of damaged insect tissue to bleach methylene blue. 10. The blood of insects normally contains dissolved oxygen in equilibrium with the air. 11. The reaction involved in the blackening of insect blood may consume all the dissolved oxygen. 12. Previous observations on fluctuations in the oxidation-reduction potential of the blood of Calliphora larvae with age are probably due to changes in the rate at which oxygen is consumed by the blood after it is shed. 13. There does not therefore appear to be any valid evidence that tyrosinase is inhibited in vivo by the action of dehydrogenases. The absence of tyrosinase activity in undamaged tissue is probably due to the structure of the cytoplasm, which keeps enzyme and substrate apart. 14. Instances of the inhibition of tyrosinase reported in Crustacea and Echinodermata seem to be susceptible of the same explanation as in insects. 15. The supposed inhibitory effect of sulphydryl groups reported for vertebrate melanophores is shown to be due to the combination of sulphydryl groups with o-quinones, which prevents the formation of melanins.

Induced changes in oxidation-reduction potentials, pH, and oxygen tension in the intact lactating mammary gland

1957 ◽  
Vol 146 (924) ◽  
pp. 400-415 ◽  
Keyword(s):  
Growth Hormone ◽  
Mammary Gland ◽  
Oxygen Tension ◽  
Reduction Potential ◽  
Reduction Potentials ◽  
Oxidation Reduction ◽  
Oxidation Reduction Potential ◽  
Lactating Mammary Gland ◽  
Other Substances

This work was undertaken to find if a study of oxidation-reduction potentials, pH and oxygen tension would yield information concerning physiological changes induced in lactating mammary glands of rats and rabbits by hormones and other substances. Breathing oxygen at atmospheric pressure caused a rapid rise in oxygen tension in lactating mammary gland, and a small, slower rise of oxidation-reduction potential. Breathing nitrogen had the opposite effect. Oxytocin caused a rapid temporary fall of oxidation-reduction potential, synchronous with milk ejection. With adrenaline the response was more rapid and the oxygen tension fell to zero, to recover within 2 min. Vasopressin produced a slower fall and recovery. Insulin (35 μ g/kg) caused a preliminary rise of oxidation-reduction potential, followed by a fall lasting 1 h. The fall could be largely abolished by glucose. The synthetic oestrogen doisynolic acid caused a triphasic response in the oxidation-reduction potentials and increased oxygen tension in the gland. It reduced, but did not abolish, the changes due to insulin. Desoxycorticosterone glucoside caused a slow rise of oxidation-reduction potential, but did not alter the response to insulin. Intermedin caused a small rise of oxidation-reduction potential. The effects produced by commercial ACTH may have been due to the oxytocin and intermedin present. Growth hormone induced a small diphasic change in the oxidation-reduction potentials. The radiosensitizers tetrasodium 2-methyl-1:4-naphthohydroquinone diphosphate and tetrasodium trimethyl-hydroquinone diphosphate produced marked falls of oxidation-reduction potential even with small doses, without change of oxygen tension. Intravenous potassium ferricyanide appeared to liberate oxygen from haemoglobin in vivo . The pH of mammary gland became slightly more acid after breathing oxygen, and also in the preliminary response to insulin, glucose, doisynolic acid and desoxycorticosterone glucoside; and after oxytocin, vasopressin and growth hormone.

Modelling of Chromium Hexavalent Reduction by Ferrous Ion in a Batch Stirred Tank

2009 ◽  
Vol 4 (1) ◽  
Author(s):  
Mohamed Kheireddine Aroua ◽  
Chun Ming Chew ◽  
Mohamed Azlan Hussain
Keyword(s):  
Reduction Process ◽  
Stirred Tank ◽  
Quasi Equilibrium ◽  
Time Data ◽  
Equilibrium Conditions ◽  
Oxidation Reduction ◽  
Oxidation Reduction Potential ◽  
On Line ◽  
And Control ◽  
Non Equilibrium

In this paper mathematical models are proposed to simulate the dynamic behaviour of the reduction of Cr(VI) with Fe(II) in a batch stirred tank. Two approaches have been adopted to represent systems in non-equilibrium and quasi-equilibrium conditions. The models derived reflect the changes of the relevant reactant species concentration based on the Oxidation Reduction Potential (ORP) of the system. An automated pilot plant has been designed and commissioned to carry out on-line/real time data acquisition and control for this Cr(VI) reduction process experimentally. Both the quasi-equilibrium and non-equilibrium models were validated experimentally. Simulated and on-line results indicate distinctive ORP profiles pattern for these two processes. The ORP profiles for non-equilibrium processes show a distinctive pattern which indicates the complete reduction of Cr(VI) in the batch sample.

scholarly journals ESTIMATION OF EFFICIENCY OF FISH GROWING IN WATER WITH NEGATIVE OXIDATION-REDUCTION POTENTIAL

2020 ◽  
Vol 2020 (3) ◽  
pp. 103-110
Author(s):  
Anna Aleksandrovna Bakhareva ◽  
Yulia Nikolaevna Grozesku ◽  
Adelya Dzumaniyashevna Zhandalgarova ◽  
Leonid Matveevich Slavin ◽  
Aleksandr Nevalennyy
Keyword(s):  
Redox Potential ◽  
Physiological State ◽  
Electrical Potential ◽  
High Growth ◽  
High Growth Rate ◽  
Oxidation Reduction ◽  
Structure Changes ◽  
Oxidation Reduction Potential ◽  
Standard Values ◽  
And Control

The paper outlines the problems of fish rearing in the water with negative redox potential. Water is known to consist of supermolecules, the so-called clusters and cells, connected by a special molecular structure. This structure changes when exposed to water in different ways: chemical, electromagnetic, mechanical. Under these influences the water molecules rearrange forming the structured living water. The effectiveness of activated water is explained by the fact that in the process of electrolysis the dead water acquires a positive electric potential, and living water – a negative electrical potential. It becomes a weak electrolyte which quickly interacts with body fluids (gastric juice, blood, lymph, intercellular fluid, etc.). As a result of the studies carried out on juvenile tilapia, it has been found that the hydrochemical parameters in the experimental (growing in structured water) and control variants were within the standard values. Concentration of O2 made 7 mg/l, pH of the medium was 7.2, and nitrite concentration was 0.3 mg/l. It has been inferred that the redox potential (redox) of the blood of young tilapia has a negative value (–96 mV). It has been recorded that at a water temperature of 21°C that is sufficiently low for thermophilic tilapia and at a negative redox (–212 mV) there has been observed a high growth rate of fish. It was found that the use of redox water (–100 mV) is most effective, because the linear-weight gain of juveniles increases by 41% in comparison with the control. Negatively charged (cathode) water increases the efficiency of rearing tilapia fry and does not affect the physiological state of fish. Tilapia fry reared in the structured water have the highest hemoglobin content (62-64 g/l) and total protein (13.3-14.3 g/l).

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