Actions of activin A, connective tissue growth factor, hepatocyte growth factor and teratocarcinoma-derived growth factor 1 on the development of the bovine preimplantation embryo

2017 ◽  
Vol 29 (7) ◽  
pp. 1329 ◽  
Author(s):  
Jasmine Kannampuzha-Francis ◽  
Paula Tribulo ◽  
Peter J. Hansen
Keyword(s):  
Growth Factor ◽  
Connective Tissue ◽  
Hepatocyte Growth Factor ◽  
Connective Tissue Growth Factor ◽  
Activin A ◽  
Blastocyst Stage ◽  
Tissue Growth ◽  
Bioactive Molecules ◽  
Bovine Embryo ◽  
Hepatocyte Growth

The reproductive tract secretes bioactive molecules collectively known as embryokines that can regulate embryonic growth and development. In the present study we tested four growth factors expressed in the endometrium for their ability to modify the development of the bovine embryo to the blastocyst stage and alter the expression of genes found to be upregulated (bone morphogenetic protein 15 (BMP15) and keratin 8, type II (KRT8)) or downregulated (NADH dehydrogenase 1 (ND1) and S100 calcium binding protein A10 (S100A10)) in embryos competent to develop to term. Zygotes were treated at Day 5 with 0.01, 0.1 or 1.0 nM growth factor. The highest concentration of activin A increased the percentage of putative zygotes that developed to the blastocyst stage. Connective tissue growth factor (CTGF) increased the number of cells in the inner cell mass (ICM), decreased the trophectoderm : ICM ratio and increased blastocyst expression of KRT8 and ND1. The lowest concentration of hepatocyte growth factor (HGF) reduced the percentage of putative zygotes becoming blastocysts. Teratocarcinoma-derived growth factor 1 increased total cell number at 0.01 nM and expression of S100A10 at 1.0 nM, but otherwise had no effects. Results confirm the prodevelopmental actions of activin A and indicate that CTGF may also function as an embryokine by regulating the number of ICM cells in the blastocyst and altering gene expression. Low concentrations of HGF were inhibitory to development.

Intracrine signalling of activin A in hepatocytes upregulates connective tissue growth factor (CTGF/CCN2) expression

2008 ◽  
Vol 28 (9) ◽  
pp. 1207-1216 ◽  
Author(s):  
Olav A. Gressner ◽  
Birgit Lahme ◽  
Monika Siluschek ◽  
Katharina Rehbein ◽  
Ralf Weiskirchen ◽  
...  
Keyword(s):  
Growth Factor ◽  
Connective Tissue ◽  
Connective Tissue Growth Factor ◽  
Activin A ◽  
Tissue Growth ◽  
Ccn2 Expression

scholarly journals Activin A-Smad Signaling Mediates Connective Tissue Growth Factor Synthesis in Liver Progenitor Cells

2016 ◽  
Vol 17 (3) ◽  
pp. 408 ◽  
Author(s):  
Ze-Yang Ding ◽  
Guan-Nan Jin ◽  
Wei Wang ◽  
Yi-Min Sun ◽  
Wei-Xun Chen ◽  
...  
Keyword(s):  
Growth Factor ◽  
Connective Tissue ◽  
Progenitor Cells ◽  
Connective Tissue Growth Factor ◽  
Activin A ◽  
Tissue Growth ◽  
Smad Signaling

93 DEVELOPMENT OF BOVINE PRE-IMPLANTATION EMBRYOS IS ALTERED BY ADDITION OF ACTIVIN AND CONNECTIVE TISSUE GROWTH FACTOR FROM DAYS 5 TO 7 AFTER FERTILIZATION

2016 ◽  
Vol 28 (2) ◽  
pp. 176
Author(s):  
J. Kannampuzha-Francis ◽  
P. J. Hansen
Keyword(s):  
Growth Factor ◽  
Connective Tissue ◽  
Connective Tissue Growth Factor ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Cell Number ◽  
Tissue Growth ◽  
Bioactive Molecules ◽  
Bovine Embryos

The reproductive tract secretes bioactive molecules collectively known as embryokines that can regulate embryonic growth and development. Here we tested actions of two molecules that are highly expressed in the endometrium for actions to modify development of bovine embryos. The molecules tested were activin and connective tissue growth factor (CTGF), and endpoints were percentage of fertilized oocytes becoming blastocysts and number of inner cell mass (ICM) and trophectoderm (TE) cells in Day 7 blastocysts. Bovine embryos produced in vitro from slaughterhouse ovaries were cultured in a serum-free culture medium. On Day 5 of culture, culture drops were supplemented with vehicle (control), human recombinant activin or CTGF (10–11, 10–10, or 10–9 M). On Day 7, blastocysts (n = 202) were collected and labelled with a nuclear dye (Hoescht 33342) and a TE cell marker (anti-CDX2). Statistical analysis was performed using the GLM procedure of SAS. Results are shown as least squares means ± s.e.M. The percentage of putative zygotes becoming a blastocyst on Day 7 was 28.4 ± 1.5% for control embryos; 32.2 ± 1.5%, 30.7 ± 1.5%, and 33.1 ± 1.5% for embryos cultured with 10–11, 10–10, and 10–9 M activin, respectively; and 27.8 ± 1.5%, 25.1 ± 1.5%, and 28.4 ± 1.5% for embryos cultured with 10–11, 10–10, and 10–9 M CTGF, respectively. Activin increased the proportion of putative zygotes becoming a blastocyst when added at 10–9 M (P = 0.0321) and tended to have the same effect at 10–11 M (P = 0.0898). There was no effect of any concentration of CTGF on development of putative zygotes to blastocyst on Day 7. There was no effect of treatment on total cell number or number of TE or of activin on ICM cell number or TE : ICM ratio. However, CTGF increased (P = 0.0011) the number of ICM (40.1 ± 1.5, 46.2 ± 1.4, 46.5 ± 1.4, and 56.3 ± 1.4 for control and 10–11, 10–10, and 10–9 M CTGF, respectively). CTGF also reduced (P = 0.0319) the TE : ICM ratio. Values were 2.8 ± 0.2 for control and 2.1 ± 0.2, 1.9 ± 0.2, and 1.6 ± 0.2 for 10–11, 10–10, and 10–9 M CTGF, respectively. Results indicate that activin and CTGF can affect embryo development. In particular, activin increases competence of embryos to develop to the blastocyst stage and CTGF affects blastocyst differentiation. Support was provided by NIH R03 HD080855.

Identification of connective tissue growth factor as a hepatic negative acute phase protein

Hepatology ◽  
2009 ◽  
pp. NA-NA
Author(s):  
Ieva Peredniene ◽  
Eddy van de Leur ◽  
Birgit Lahme ◽  
Monika Siluschek ◽  
Axel M. Gressner ◽  
...  
Keyword(s):  
Growth Factor ◽  
Connective Tissue ◽  
Connective Tissue Growth Factor ◽  
Acute Phase ◽  
Acute Phase Protein ◽  
Tissue Growth ◽  
Phase Protein
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