Prion protein 2 (dublet) gene (PRND): role in ovine semen capacitation, cryopreservation and fertility

2017 ◽  
Vol 29 (5) ◽  
pp. 985 ◽  
Author(s):  
L. M. Ferreira ◽  
M. Garcia-Herreros ◽  
A. Domingos ◽  
C. C. Marques ◽  
P. Mesquita ◽  
...  
Keyword(s):  
Prion Protein ◽  
Male Fertility ◽  
Gene Polymorphisms ◽  
Physiological Function ◽  
Fluorescein Isothiocyanate ◽  
Cleavage Rate ◽  
Secondary Antibodies ◽  
Fresh Semen ◽  
Low Expression

The aim of the present study was to examine the role of Doppel protein in the capacitation process and fertilising ability of both fresh and frozen–thawed (FT) spermatozoa from rams carrying different prion protein 2 (dublet) (PRND) gene polymorphisms. The detection efficacy of new anti-Doppel monoclonal antibodies and PRND mRNA quantification were also explored in ovine spermatozoa. Three different genotypes (AA, GA, GG) were identified for codon 26 of ovine PRND-c.78G>A. Using flow cytometry, a higher fluorescence was detected in fresh compared with FT sperm samples incubated with anti-Doppel primary and fluorescein isothiocyanate-conjugated secondary antibodies (P < 0.05). Capacitation was affected by semen treatment (fresh and FT) and male PRND genotype (P < 0.05). After IVF, the use of fresh semen resulted in a higher cleavage rate than the use of FT spermatozoa (P = 0.004). IVF using spermatozoa from individuals classified as carriers of the AA or GA PRND genotypes resulted in higher cleavage rates than seen using spermatozoa from GG carriers (P ≤ 0.0006). Finally, using semen from rams with the AA PRND genotype resulted in the highest Day 6 and Day 8 embryo rates (P ≤ 0.04). In conclusion, the results of the present study confirm that the identification of different PRND genotypes is important for studying the sperm capacitation process and for improving sperm cryoresistance and embryo production. Furthermore, the detection of Doppel in ejaculated ovine spermatozoa, along with its low expression after cryopreservation, strongly suggests an important physiological function of this protein in male fertility.

Phase separation of a yeast prion protein promotes cellular fitness

Science ◽  
2018 ◽  
Vol 359 (6371) ◽  
pp. eaao5654 ◽  
Author(s):  
Titus M. Franzmann ◽  
Marcus Jahnel ◽  
Andrei Pozniakovsky ◽  
Julia Mahamid ◽  
Alex S. Holehouse ◽  
...  
Keyword(s):  
Phase Separation ◽  
Prion Protein ◽  
Physiological Function ◽  
Ph Sensor ◽  
Yeast Prion ◽  
Rapid Adaptation ◽  
Yeast Prions ◽  
Stress Sensors ◽  
Domain Phase

Despite the important role of prion domains in neurodegenerative disease, their physiological function has remained enigmatic. Previous work with yeast prions has defined prion domains as sequences that form self-propagating aggregates. Here, we uncovered an unexpected function of the canonical yeast prion protein Sup35. In stressed conditions, Sup35 formed protective gels via pH-regulated liquid-like phase separation followed by gelation. Phase separation was mediated by the N-terminal prion domain and regulated by the adjacent pH sensor domain. Phase separation promoted yeast cell survival by rescuing the essential Sup35 translation factor from stress-induced damage. Thus, prion-like domains represent conserved environmental stress sensors that facilitate rapid adaptation in unstable environments by modifying protein phase behavior.

scholarly journals UCP 2 gene polymorphisms in obesity and diabetes, and the role of UCP 2 in cancer

FEBS Letters ◽  
2019 ◽  
Vol 593 (18) ◽  
pp. 2525-2534 ◽  
Author(s):  
Jinran Li ◽  
Rihua Jiang ◽  
Xianling Cong ◽  
Yunfeng Zhao
Keyword(s):  
Gene Polymorphisms ◽  
Obesity And Diabetes

scholarly journals Selenium and Dogs: A Systematic Review

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 418
Author(s):  
Viola Zentrichová ◽  
Alena Pechová ◽  
Simona Kovaříková
Keyword(s):  
Male Fertility ◽  
Clinical Signs ◽  
Complex Data ◽  
Selenium Status ◽  
Dog Food ◽  
Balanced Diet ◽  
Naturally Occurring ◽  
Correct Function ◽  
Indirect Assay

The intent of this review is to summarize the knowledge about selenium and its function in a dog’s body. For this purpose, systematic literature search was conducted. For mammals, including dogs, a balanced diet and sufficient intake of selenium are important for correct function of metabolism. As for selenium poisoning, there are no naturally occurring cases known. Nowadays, we do not encounter clinical signs of its deficiency either, but it can be subclinical. For now, the most reliable method of assessing selenium status of a dog is measuring serum or plasma levels. Levels in full blood can be measured too, but there are no reference values. The use of glutathione peroxidase as an indirect assay is questionable in canines. Commercial dog food manufactures follow recommendations for minimal and maximal selenium levels and so dogs fed commercial diets should have balanced intake of selenium. For dogs fed home-made diets, complex data are missing. However, subclinical deficiency seems to affect, for example, male fertility or recovery from parasitical diseases. Very interesting is the role of selenium in prevention and treatment of cancer.

scholarly journals Hyperactivated RAGE in Comorbidities as a Risk Factor for Severe COVID-19—The Role of RAGE-RAS Crosstalk

Biomolecules ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 876
Author(s):  
Sara Chiappalupi ◽  
Laura Salvadori ◽  
Rosario Donato ◽  
Francesca Riuzzi ◽  
Guglielmo Sorci
Keyword(s):  
Gene Polymorphisms ◽  
Renin Angiotensin System ◽  
Molecular Target ◽  
Advanced Glycation ◽  
Angiotensin System ◽  
Major Player ◽  
Glycation End Products ◽  
End Products ◽  
And Oxidative Stress

The receptor for advanced glycation-end products (RAGE) is a multiligand receptor with a role in inflammatory and pulmonary pathologies. Hyperactivation of RAGE by its ligands has been reported to sustain inflammation and oxidative stress in common comorbidities of severe COVID-19. RAGE is essential to the deleterious effects of the renin–angiotensin system (RAS), which participates in infection and multiorgan injury in COVID-19 patients. Thus, RAGE might be a major player in severe COVID-19, and appears to be a useful therapeutic molecular target in infections by SARS-CoV-2. The role of RAGE gene polymorphisms in predisposing patients to severe COVID-19 is discussed. 

Association of TLR4 gene polymorphisms with sepsis after a burn injury: findings of the functional role of rs2737190 SNP

2021 ◽  
Author(s):  
Claudia A. Colín-Castro ◽  
Rafael Franco-Cendejas ◽  
Hector I. Rocha-González ◽  
Esteban Cruz-Arenas ◽  
Norberto Leyva-García ◽  
...  
Keyword(s):  
Gene Polymorphisms ◽  
Functional Role ◽  
Burn Injury ◽  
Tlr4 Gene

scholarly journals Kindlin-2 in Sertoli cells is essential for testis development and male fertility in mice

2021 ◽  
Vol 12 (6) ◽  
Author(s):  
Xiaochun Chi ◽  
Weiwei Luo ◽  
Jiagui Song ◽  
Bing Li ◽  
Tiantian Su ◽  
...  
Keyword(s):  
Sertoli Cells ◽  
Male Fertility ◽  
Nuclear Translocation ◽  
Hippo Pathway ◽  
Reproductive Organs ◽  
Male Reproduction ◽  
Barrier Structure ◽  
Male Mice ◽  
Sperm Development

AbstractKindlin-2 is known to play important roles in the development of mesoderm-derived tissues including myocardium, smooth muscle, cartilage and blood vessels. However, nothing is known for the role of Kindlin-2 in mesoderm-derived reproductive organs. Here, we report that loss of Kindlin-2 in Sertoli cells caused severe testis hypoplasia, abnormal germ cell development and complete infertility in male mice. Functionally, loss of Kindlin-2 inhibits proliferation, increases apoptosis, impairs phagocytosis in Sertoli cells and destroyed the integration of blood-testis barrier structure in testes. Mechanistically, Kindlin-2 interacts with LATS1 and YAP, the key components of Hippo pathway. Kindlin-2 impedes LATS1 interaction with YAP, and depletion of Kindlin-2 enhances LATS1 interaction with YAP, increases YAP phosphorylation and decreases its nuclear translocation. For clinical relevance, lower Kindlin-2 expression and decreased nucleus localization of YAP was found in SCOS patients. Collectively, we demonstrated that Kindlin-2 in Sertoli cells is essential for sperm development and male reproduction.

scholarly journals Role of Selenium and Selenoproteins in Male Reproductive Function: A Review of Past and Present Evidences

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 268 ◽  
Author(s):  
Izhar Hyder Qazi ◽  
Christiana Angel ◽  
Haoxuan Yang ◽  
Evangelos Zoidis ◽  
Bo Pan ◽  
...  
Keyword(s):  
Male Fertility ◽  
Defense Mechanisms ◽  
Antioxidant Defense ◽  
Biological Effects ◽  
Reproductive Function ◽  
Vital Role ◽  
Biologically Relevant ◽  
Male Reproductive Function ◽  
The Subject

Selenium (Se) is an important trace mineral having many essential roles at the cellular and organismal levels in animal and human health. The biological effects of Se are mainly carried out by selenoproteins (encoded by 25 genes in humans and 24 in mice). As an essential component of selenoproteins, Se performs structural and enzymic roles; in the latter context it is well known for its catalytic and antioxidative functions. Studies involving different animal models have added great value to our understanding regarding the potential implications of Se and selenoproteins in mammalian fertility and reproduction. In this review, we highlight the implications of selenoproteins in male fertility and reproduction followed by the characteristic biological functions of Se and selenoproteins associated with overall male reproductive function. It is evident from observations of past studies (both animal and human) that Se is essentially required for spermatogenesis and male fertility, presumably because of its vital role in modulation of antioxidant defense mechanisms and other essential biological pathways and redox sensitive transcription factors. However, bearing in mind the evidences from mainstream literature, it is also advisable to perform more studies focusing on the elucidation of additional roles played by the peculiar and canonical selenoproteins i.e., glutathione peroxidase 4 (GPX4) and selenoprotein P (SELENOP) in the male reproductive functions. Nevertheless, search for the elucidation of additional putative mechanisms potentially modulated by other biologically relevant selenoproteins should also be included in the scope of future studies. However, as for the implication of Se in fertility and reproduction in men, though a few clinical trials explore the effects of Se supplementation on male fertility, due to inconsistencies in the recruitment of subjects and heterogeneity of designs, the comparison of such studies is still complicated and less clear. Therefore, further research focused on the roles of Se and selenoproteins is awaited for validating the evidences at hand and outlining any therapeutic schemes intended for improving male fertility. As such, new dimensions could be added to the subject of male fertility and Se supplementation.

scholarly journals The Role of CatSper1 and CatSper2 Ion Channels in Male Fertility and Infertility

2015 ◽  
Vol 10 ◽  
pp. 730-736 ◽  
Author(s):  
Saikat Saha ◽  
Keka Talukdar ◽  
Amit K. Chakraborty
Keyword(s):  
Ion Channels ◽  
Male Fertility

A Study on the Role of Estrogen Receptor Gene Polymorphisms in Female Infertility

2016 ◽  
Vol 20 (11) ◽  
pp. 692-695 ◽  
Author(s):  
Monisha Swaminathan ◽  
Vijaya Ganesh ◽  
Teena Koshy ◽  
Priyanka Venugopal ◽  
Solomon Paul ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Gene Polymorphisms ◽  
Receptor Gene ◽  
Female Infertility ◽  
Estrogen Receptor Gene ◽  
Receptor Gene Polymorphisms
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