Prostaglandin and progesterone production by bovine luteal cells incubated in the presence or absence of the accessory cells of the corpus luteum and treated with interleukin-1β, indomethacin and luteinizing hormone

1997 ◽  
Vol 9 (6) ◽  
pp. 651 ◽  
Author(s):  
R. P. Del Vecchio ◽  
W. D. Sutherland
Keyword(s):  
Luteinizing Hormone ◽  
Corpus Luteum ◽  
Interleukin 1Β ◽  
Luteal Cells ◽  
Progesterone Production ◽  
Accessory Cells ◽  
Pge2 Release

This experiment examined production of prostaglandin (PG) F2α , PGE2 and progesterone by bovine luteal cells incubated with or without the accessory cells and treated with recombinant bovine interleukin-1β (10 ng), indomethacin (5 µg) or luteinizing hormone (50 ng). During pretreatment, progesterone production was similar in the luteal and luteal plus accessory cells, being greater in either of these than in accessory cells; PGF2α was greatest in luteal plus accessory, intermediate in accessory and lowest in the luteal cells; PGE2 was similar among all cell arrangements. Luteinizing hormone or luteinizing hormone plus indomethacin stimulated progesterone in the luteal and luteal plus accessory cells, this being similar in these two cell arrangements. Interleukin-1β stimulated PGF2α in luteal and luteal plus accessory cells, and tended to elevate PGF2α in accessory cells. Interleukin-1β stimulated PGE2 in all cell arrangements. Indomethacin inhibited the interleukin-1b-stimulated PGF2α and PGE2 release. Indomethacin or indomethacin plus luteinizing hormone inhibited basal PGE2 in luteal and luteal plus accessory cells. These data suggest that progesterone production is not inßuenced by the presence of accessory cells of the corpus luteum, that accessory and luteal cells produce appreciable amounts of PG, and that recombinant bovine interleukin-1b stimulates PGE2 and PGF2α in the luteal and accessory cells.

Oxytocin may play a role in the control of the human corpus luteum

1982 ◽  
Vol 95 (1) ◽  
pp. 65-70 ◽  
Author(s):  
G. J. S. Tan ◽  
R. Tweedale ◽  
J. S. G. Biggs
Keyword(s):  
Menstrual Cycle ◽  
Corpus Luteum ◽  
Inhibitory Action ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Corpora Lutea ◽  
Luteal Cells ◽  
Progesterone Production ◽  
Human Corpus Luteum

The effects of oxytocin on dispersed luteal cells from human corpora lutea of the menstrual cycle were studied. Oxytocin at a concentration of 4 mi.u./ml produced a slight increase in basal progesterone production. However, higher oxytocin concentrations (400 and 800 mi.u./ml) markedly inhibited both basal and human chorionic gonadotrophin-induced progesterone production. These data provide evidence for an effect of oxytocin on the human corpus luteum. In view of the inhibitory action of oxytocin, increased secretion of this hormone may be important in the demise of the corpus luteum at the end of the menstrual cycle.

In. vitro evaluation of corpus luteum function of cycling and pregnant rhesus monkeys: Progesterone production by dispersed luteal cells

Steroids ◽  
1976 ◽  
Vol 27 (4) ◽  
pp. 543-551 ◽  
Author(s):  
Richard L. Stouffer ◽  
Wilbert E. Nixon ◽  
Bela J. Gulyas ◽  
David.K. Johnson ◽  
Gary D. Hodgen
Keyword(s):  
Corpus Luteum ◽  
Rhesus Monkeys ◽  
In Vitro Evaluation ◽  
Luteal Cells ◽  
Progesterone Production

scholarly journals Interleukin-1β Stimulates Progesterone Production byin VitroHuman Luteal Cells: Evidence of a Mediatory Role of Prostaglandins

2003 ◽  
Vol 88 (6) ◽  
pp. 2690-2694 ◽  
Author(s):  
Fiorella Miceli ◽  
Anna Tropea ◽  
Francesca Minici ◽  
Pierluigi Navarra ◽  
Antonio Lanzone ◽  
...  
Keyword(s):  
Interleukin 1Β ◽  
Luteal Cells ◽  
Progesterone Production

Effect of ovine conceptus and endometrial secretory products on luteal progesterone production in vitro

1986 ◽  
Vol 64 (12) ◽  
pp. 1556-1560
Author(s):  
D. W. Beckner ◽  
J. G. Manns
Keyword(s):  
Luteinizing Hormone ◽  
Conditioned Medium ◽  
Endometrial Tissue ◽  
Minimal Essential Medium ◽  
Luteal Cells ◽  
Endometrial Cells ◽  
Progesterone Production ◽  
Luteal Tissue ◽  
Secretory Products

The objectives of this study were the following: (i) to determine if ovine conceptus secretory products are directly luteotrophic to luteal tissue in vitro and (ii) to determine if ovine conceptus secretory products stimulate endometrial tissue to secrete a luteotropin in vitro. Conceptus-conditioned medium (CCM) was prepared by incubating day 14 ovine conceptuses in minimal essential medium (MEM) for 24 h and harvesting the supernatant. Endometrium-conditioned CCM (E-CCM) and endometrium-conditioned medium (ECM) were prepared by incubating dispersed ovine endometrial cells from day 9–10 cycling ewes in CCM or MEM, respectively, for 16 h and harvesting the supernatants. Media, conditioned as described, were incubated at various dilutions with dispersed luteal cells from day 9–10 cycling ewes for 90 min or 6 h in the absence or presence of 50 ng/mL ovine luteinizing hormone (oLH). CCM did not alter progesterone (P4) production in the 90-min incubation but did increase (p < 0.05) P4 production in the 6-h incubation (1:4, 1:8, 1:16 dilutions). When coincubated with oLH, CCM did not increase P4 production above that stimulated by oLH alone. The effect of E-CCM was similar to CCM or ECM and did not differ significantly from basal. It is concluded that the day 14 ovine conceptus does secrete a factor that is able to directly stimulate P4 secretion by luteal cells in a 6-h, but not a 90-min, incubation. Conceptus secretory products did not stimulate endometrial cells to secrete a luteotropin. The data are consistent with the suggestion that the luteotrophic factor secreted by the conceptus is a prostaglandin of the E series.

Lack of direct inhibitory action of oxytocin on progesterone production by dispersed cells from human corpus luteum

1985 ◽  
Vol 104 (1) ◽  
pp. 149-151 ◽  
Author(s):  
M. C. Richardson ◽  
G. M. Masson
Keyword(s):  
Corpus Luteum ◽  
Luteal Phase ◽  
Inhibitory Action ◽  
Corpora Lutea ◽  
Luteal Cells ◽  
Menstrual Cycles ◽  
Progesterone Production ◽  
Human Corpus Luteum ◽  
Dispersed Cells

ABSTRACT Suspensions of luteal cells were prepared from samples of human corpora lutea obtained during the luteal phase of menstrual cycles. Addition of oxytocin (1 μmol/l) to the various cell preparations had no effect on either basal production of progesterone or on steroidogenic responses to a range of concentrations of gonadotrophin. J. Endocr. (1985) 104, 149–151

Luteinizing Hormone Up-Regulates the Expression of Interleukin-1β mRNA in Human Granulosa-Luteal Cells

2000 ◽  
Vol 43 (3) ◽  
pp. 125-133 ◽  
Author(s):  
HSIN-FU CHEN ◽  
KUANG-HAN CHAO ◽  
LI-JUNG CHANG ◽  
HONG-NERNG HO ◽  
YU-SHIH YANG ◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Interleukin 1Β ◽  
Luteal Cells

Inhibitory action of chemically deglycosylated human chorionic gonadotrophin on hormone-induced steroid production by dispersed cells from human corpus luteum

1984 ◽  
Vol 101 (3) ◽  
pp. 327-332 ◽  
Author(s):  
M. C. Richardson ◽  
G. M. Masson ◽  
M. R. Sairam
Keyword(s):  
Corpus Luteum ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Maximum Response ◽  
Luteal Cells ◽  
Progesterone Production ◽  
Anhydrous Hydrogen Fluoride ◽  
Tenfold Excess ◽  
Human Corpus Luteum ◽  
Dispersed Cells

ABSTRACT The biological activity of deglycosylated human chorionic gonadotrophin (hCG) prepared by treatment of the native hormone with anhydrous hydrogen fluoride was evaluated using suspensions of dispersed cells from biopsies of human corpus luteum obtained during the luteal phase of normal menstrual cycles. A reproducible pattern of response to hCG in terms of progesterone production by luteal cells was established for a range of luteal ages. Deglycosylation of hCG led to a diminished level of maximum response to the hormone. Co-incubation of luteal cells with a level of hCG just sufficient to elicit a maximum response and increasing concentrations of deglycosylated hCG led to a progressive inhibition of the hormonal response; at a concentration of 103 ng deglycosylated hCG/ml (a tenfold excess of deglycosylated hCG over the native hormone), hCG-induced progesterone production was reduced by about 50%. Deglycosylated hCG therefore acts as a partial antagonist for the action of hCG on human luteal cells. J. Endocr. (1984) 101, 327–332

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