Mutational Analysis of Root Gravitropism and Phototropism of Arabidopsis thaliana Seedlings

1992 ◽  
Vol 19 (4) ◽  
pp. 439 ◽  
Author(s):  
K Okada ◽  
Y Shimura
Keyword(s):  
Arabidopsis Thaliana ◽  
Mutational Analysis ◽  
Agar Medium ◽  
Simple Procedure ◽  
Genetic Regulation ◽  
Response Type ◽  
Phototropic Response ◽  
Light Stimuli ◽  
Root Gravitropism ◽  
Complementation Groups

Root gravitropism and phototropism were analysed in young seedlings of Arabidopsis thaliana by a simple procedure using agar plates, by changing the position of plates or by covering the plates with black opaque sheet. Roots of A. thaliana seedlings growing on the surface of agar medium showed clear responses to gravity and light stimuli. A set of mutants with abnormal gravitropism or phototropism was isolated. Gravitropic mutants were separated into two types, reduced-response type and non-response type. The mutants were genetically divided into two complementation groups, aux1 and agr1. In both groups the reduced-response type mutants carried weaker genetic lesions, whereas the non-response type mutants carried heavier genetic lesions. Mutants with no phototropic response were classified into two complementation groups, rpt1 and rpt2. The root tropism mutants showed normal tropic responses in shoots, indicating that genetic regulation pathways are different in roots and shoots.

scholarly journals A Mutational Analysis of Leaf Morphogenesis in Arabidopsis thaliana

Genetics ◽  
1999 ◽  
Vol 152 (2) ◽  
pp. 729-742 ◽  
Author(s):  
Genoveva Berná ◽  
Pedro Robles ◽  
José Luis Micol
Keyword(s):  
Arabidopsis Thaliana ◽  
Large Scale ◽  
Mutational Analysis ◽  
Leaf Ontogeny ◽  
Leaf Morphogenesis ◽  
Complete Penetrance ◽  
Complementation Groups ◽  
Mutant Phenotypes ◽  
Leaf Mutants ◽  
Large Scale Screening

Abstract As a contribution to a better understanding of the developmental processes that are specific to plants, we have begun a genetic analysis of leaf ontogeny in the model system Arabidopsis thaliana by performing a large-scale screening for mutants with abnormal leaves. After screening 46,159 M2 individuals, arising from 5770 M1 parental seeds exposed to EMS, we isolated 1926 M2 putative leaf mutants, 853 of which yielded viable M3 inbred progeny. Mutant phenotypes were transmitted with complete penetrance and small variations in expressivity in 255 lines. Most of them were inherited as recessive monogenic traits, belonging to 94 complementation groups, which suggests that we did not reach saturation of the genome. We discuss the nature of the processes presumably perturbed in the phenotypic classes defined among our mutants.

scholarly journals MUTATIONAL ANALYSIS OF THE REGION SURROUNDING THE 93D HEAT SHOCK LOCUS OF DROSOPHILA MELANOGASTER

Genetics ◽  
1984 ◽  
Vol 106 (2) ◽  
pp. 249-265
Author(s):  
Jym Mohler ◽  
Mary Lou Pardue
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Mutational Analysis ◽  
Point Mutations ◽  
Γ Irradiation ◽  
Lethal Mutations ◽  
Complementation Groups ◽  
In Trans ◽  
Shock Locus ◽  
Shock Proteins

ABSTRACT The region containing subdivisions 93C, 93D and 93E on chromosome 3 of Drosophila melanogaster has been screened for visible and lethal mutations. Treatment with three mutagens, γ irradiation, ethyl methanesulfonate and diepoxybutane, has produced mutations that fall into 20 complementation groups, including the previously identified ebony locus. No point mutations affecting the heat shock locus in 93D were detected; however, a pair of deficiencies that overlap in the region of this locus was isolated. Flies heterozygous in trans for this pair of deficiencies are capable of producing all of the major heat shock puffs (except 93D) and the major heat shock proteins. In addition, these flies show recovery of normal protein synthesis following a heat shock.

scholarly journals A type III ACC synthase, ACS7, is involved in root gravitropism in Arabidopsis thaliana

2013 ◽  
Vol 64 (14) ◽  
pp. 4343-4360 ◽  
Author(s):  
Shih-Jhe Huang ◽  
Chia-Lun Chang ◽  
Po-Hsun Wang ◽  
Min-Chieh Tsai ◽  
Pang-Hung Hsu ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Acc Synthase ◽  
Type Iii ◽  
Root Gravitropism

scholarly journals CYTOGENETIC ANALYSIS OF THE CHROMOSOMAL REGION IMMEDIATELY ADJACENT TO THE ROSY LOCUS IN DROSOPHILA MELANOGASTER

Genetics ◽  
1980 ◽  
Vol 95 (1) ◽  
pp. 95-110 ◽  
Author(s):  
Arthur J Hilliker ◽  
Stephen H Clark ◽  
Arthur Chovnick ◽  
William M Gelbart
Keyword(s):  
Drosophila Melanogaster ◽  
Polytene Chromosome ◽  
Structural Information ◽  
Genetic Regulation ◽  
Chromosome Band ◽  
Genetic Dissection ◽  
Genetic Unit ◽  
Complementation Groups ◽  
The Relationship ◽  
Chromosome Bands

ABSTRACT This report describes the genetic analysis of a region of the third chromosome of Drosophila melanogaster extending from 87D2-4 to 87E12-F1, an interval of 23 or 24 polytene chromosome bands. This region includes the rosy (ry, 3-52.0) locus, carrying the structural information for xanthine dehydrogenase (XDH). We have, in recent years, focused attention on the genetic regulation of the rosy locus and, therefore, wished to ascertain in detail the immediate genetic environmcnt of this locus. Specifically, we question if rosy is a solitary genetic unit or part of a larger complex genetic unit encompassing adjacent genes. Our data also provide opportunity to examine further the relationship between euchromatic gene distrihution and polytene chromosome structure.—The results of our genetic dissection of the rosy microregion substantiate the conclusion drawn earlier (SCHALET, KERNAGHAN and CHOVNICK 1964) that the rosy locus is the only gene in this region concerned with XDH activity and that all adjacent genetic units are functionally, as well as spatially, distinct Erom the rosy gene. Within the rosy micro-region, we observed a close correspondence between the number of complementation groups (21) and the number of polytene chromosome bands (23 or 24). Consideration of this latter observation in conjunction with those of similar studies of other chhromosomal regions supports the hypothesis that each polytene chromosome band corresponds to a single genetic unit.

scholarly journals The Cyclic Nucleotide-Gated Channel CNGC14 Regulates Root Gravitropism in Arabidopsis thaliana

2015 ◽  
Vol 25 (23) ◽  
pp. 3119-3125 ◽  
Author(s):  
Han-Wei Shih ◽  
Cody L. DePew ◽  
Nathan D. Miller ◽  
Gabriele B. Monshausen
Keyword(s):  
Arabidopsis Thaliana ◽  
Cyclic Nucleotide ◽  
Gated Channel ◽  
Root Gravitropism

Root Morphology Mutants in Arabidopsis thaliana

1992 ◽  
Vol 19 (4) ◽  
pp. 427 ◽  
Author(s):  
TI Baskin ◽  
AS Betzner ◽  
R Hoggart ◽  
A Cork ◽  
RE Williamson
Keyword(s):  
Arabidopsis Thaliana ◽  
Root Growth ◽  
Mutational Analysis ◽  
Root Apex ◽  
Temperature Sensitive ◽  
Restrictive Temperature ◽  
Wild Type ◽  
In The Wild ◽  
Root Morphogenesis ◽  
Type Rate

We have begun a mutational analysis of root morphogenesis in Arabidopsis thaliana. We report here the initial genetic and physiological characterisation of six mutations that affect root growth and development. Three of them (rsw1, rsw2, rsw3) cause extensive radial swelling of the root apex. These mutations are recessive at different loci and show temperature-sensitive expression, such that the roots appear wild type when grown at 18�C but express the mutant phenotype when transferred to 31�C. Following transfer to the restrictive temperature, these three mutations have different kinetic and morphological patterns of radial swelling, and grow at different rates with continued time at high temperature. We believe that these mutations represent three different loci active in the wild type in regulating the shape of the root. We have also characterised two mutations that affect only the root epidermis, causing many epidermal cells to bulge (reb1-1, reb1-2). The two mutations are recessive and are alleles. However, rebl-1 is constitutive whereas reb1-2 is temperature sensitive, only expressing at 33�C. Reb1-2 also causes a deviation from the normal straight growth of the root such that the affected roots grow with sharp bends or meanders. The final mutant reported here is a stunted plant (stp1), in which the root growth rate is approximately 25% of the wild type rate. Moreover, root growth steadily accelerates over 5 days following germination in the wild type but remains constant in stp1, which grows at a constant rate over the same interval.

Abscisic acid is a negative regulator of root gravitropism in Arabidopsis thaliana

2009 ◽  
Vol 378 (4) ◽  
pp. 695-700 ◽  
Author(s):  
Woong Han ◽  
Honglin Rong ◽  
Hanma Zhang ◽  
Myeong-Hyeon Wang
Keyword(s):  
Arabidopsis Thaliana ◽  
Abscisic Acid ◽  
Negative Regulator ◽  
Root Gravitropism

A new Arabidopsis thaliana root gravitropism and chirality mutant

Plant Science ◽  
2000 ◽  
Vol 158 (1-2) ◽  
pp. 77-85 ◽  
Author(s):  
S Ferrari ◽  
S Piconese ◽  
G Tronelli ◽  
F Migliaccio
Keyword(s):  
Arabidopsis Thaliana ◽  
Root Gravitropism
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