Analysis of a promoter for the NADH - glutamate synthase gene in rice (Oryza sativa): cell type-specific expression in developing organs of transgenic rice plants

2000 ◽  
Vol 27 (9) ◽  
pp. 787 ◽  
Author(s):  
Soichi Kojima ◽  
Michiko Kimura ◽  
Yukine Nozaki ◽  
Tomoyuki Yamaya
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Glutamate Synthase ◽  
Gus Activity ◽  
Start Codon ◽  
Assimilate Transport ◽  
Upstream Region ◽  
Vascular Bundles ◽  
Specific Expression ◽  
Gus Reporter

This paper originates from a presentation at the International Conference on Assimilate Transport and Partitioning, Newcastle, NSW, August 1999 The entire 3.7 kbp 5´-upstream region (–2840 to +886) from the translational start codon of NADH–glutamate synthase (NADH–GOGAT, EC 1.4.1.14) gene from rice (Oryza sativa L.) or the region sequentially deleted from the 5´-end was fused with the β−glucuronidase (GUS) reporter gene. The chimeric gene was introduced into calli derived from rice scutellum via Agrobacterium tumefaciens-mediated transformation and tissue-specific GUS activity determined in T0 generations. When the entire region was fused, GUS activity was detected in vascular bundles of the developing leaf blade and in dorsal and lateral vascular bundles of developing grains. This corresponds with our previous immunodetection of NADH–GOGAT protein (Hayakawa et al., Planta 193, 455–460, 1994). A series of deletion experiments showed that a 149-nucleotide region between –142 and +7 was essential for promoter activity in the NADH–GOGAT gene.

scholarly journals Senescence-Specific Expression of RAmy1A Accelerates Non-structural Carbohydrate Remobilization and Grain Filling in Rice (Oryza sativa L.)

2021 ◽  
Vol 12 ◽  
Author(s):  
Ning Ouyang ◽  
Xuewu Sun ◽  
Yanning Tan ◽  
Zhizhong Sun ◽  
Dong Yu ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Transgenic Rice ◽  
Oryza Sativa L ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Grain Filling ◽  
Leaf Sheath ◽  
Specific Expression ◽  
Total Soluble Sugar ◽  
Transgenic Lines

Remobilization of pre-anthesis NSCs (non-structural carbohydrates) is significant for effective grain filling in rice (Oryza sativa L.). However, abundant starch particles as an important component of NSCs are still present in the leaf sheath and stem at the late stage of grain filling. There are no studies on how bioengineering techniques can be used to improve the efficiency of NSC remobilization. In this study, RAmy1A was expressed under the senescence-specific promoter of SAG12, which was designed to degrade starch in the leaf sheath and stem during grain filling. RAmy1A mRNA successfully accumulated in the leaf, stem, and sheath of transgenic plants after anthesis. At the same time, the starch and total soluble sugar content in the leaf, stem, and leaf sheath were obviously decreased during the grain-filling period. The photosynthetic rate of transgenic lines was higher than that of the wild types by an average of 4.0 and 9.9%, at 5 and 10 days after flowering, respectively. In addition, the grain-filling rate of transgenic lines was faster than that of the wild types by an average of 26.09%. These results indicate an enhanced transport efficiency of NSCs from source tissues in transgenic rice. Transgenic rice also displayed accelerated leaf senescence, which was hypothesized to contribute to decreased grain weight.

scholarly journals Mapping of QTLs associated with cytosolic glutamine synthetase and NADH‐glutamate synthase in rice ( Oryza sativa L.)

2001 ◽  
Vol 52 (359) ◽  
pp. 1209-1217 ◽  
Author(s):  
Mitsuhiro Obara ◽  
Makoto Kajiura ◽  
Yoshimichi Fukuta ◽  
Masahiro Yano ◽  
Makoto Hayashi ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Glutamine Synthetase ◽  
Oryza Sativa L ◽  
Glutamate Synthase ◽  
Cytosolic Glutamine Synthetase

scholarly journals Mapping of QTLs associated with cytosolic glutamine synthetase and NADH‐glutamate synthase in rice (Oryza sativa L.)

2001 ◽  
Vol 52 (359) ◽  
pp. 1209-1217 ◽  
Author(s):  
Mitsuhiro Obara ◽  
Makoto Kajiura ◽  
Yoshimichi Fukuta ◽  
Masahiro Yano ◽  
Makoto Hayashi ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Glutamine Synthetase ◽  
Oryza Sativa L ◽  
Glutamate Synthase ◽  
Cytosolic Glutamine Synthetase

Distribution, structure, organ-specific expression, and phylogenic analysis of the pathogenesis-related protein-3 chitinase gene family in rice (Oryza sativa L.)

Genome ◽  
2006 ◽  
Vol 49 (6) ◽  
pp. 619-630 ◽  
Author(s):  
T Nakazaki ◽  
T Tsukiyama ◽  
Y Okumoto ◽  
D Kageyama ◽  
K Naito ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Biological Effects ◽  
Plant Genome ◽  
Specific Expression ◽  
Rice Plants ◽  
Pathogenesis Related Protein ◽  
Pathogenesis Related ◽  
Organ Specific ◽  
Organ Specific Expression

Rice (Oryza sativa L.) pathogenesis-related (PR)-3 chitinases, like other PR proteins, are each coded by one of the genes of a multigene family in the plant genome. We assembled the database information about rice PR-3 chitinase sequences. A total of 12 PR-3 chitinase loci (Cht1 to Cht12) were found deployed in the rice genome. Some of the loci were occupied by 2 or more alleles. For all the loci expect Cht4, Cht5, Cht6, and Cht11, the amino acid sequence was polymorphic between japonica and indica varieties of rice, but glutamic acid acting as a catalytic residue was completely conserved in all the loci expect Cht7. All the genes except Cht7, which was not tested in this study, were transcripted in some organs (leaf, sheath, root, and meristem) of rice plants. These results suggest that chitinase proteins encoded by the genes at these loci have important biological effects, at least antifungal activities, on rice plants. We also proposed a new classification of rice PR-3 chitinases based on their domain structures. This classification was consistent with the results of phylogenetic analysis of rice chitinases.Key words: allelic relationship, classification, organ-specific expression, PR-3 chitinase, rice (Oryza sativa L.).

Promoter deletion analysis reveals root-specific expression of the alkenal reductase gene (OsAER1) in Oryza sativa

2019 ◽  
Vol 46 (4) ◽  
pp. 376
Author(s):  
Aniversari Apriana ◽  
Atmitri Sisharmini ◽  
Hajrial Aswidinnoor ◽  
Kurniawan R. Trijatmiko ◽  
Sudarsono Sudarsono
Keyword(s):  
Oryza Sativa ◽  
Transgenic Plants ◽  
Promoter Activity ◽  
Deletion Analysis ◽  
Specific Expression ◽  
Cis Acting ◽  
Promoter Fragment ◽  
Gus Reporter ◽  
Promoter Deletion Analysis

Root-specific promoters are useful in plant genetic engineering, primarily to improve water and nutrient absorption. The aim of this study was to clone and characterise the promoter of the Oryza sativa L. alkenal reductase (OsAER1) gene encoding 2-alkenal reductase, an NADPH-dependent oxidoreductase. Expression analysis using quantitative real-time PCR confirmed the root-specific expression of the OsAER1 gene. Subsequently, a 3082-bp fragment of the OsAER1 promoter was isolated from a local Indonesian rice cultivar, Awan Kuning. Sequencing and further nucleotide sequence analysis of the 3082-bp promoter fragment (PA-5) revealed the presence of at least 10 root-specific cis-regulatory elements putatively responsible for OsAER1 root-specific expression. Using the 3082-bp promoter fragment to drive the expression of the GUS reporter transgene confirmed that the OsAER1 promoter is root-specific. Further, the analysis indicated that OsAER1 promoter activity was absent in leaves, petioles and shoots during sprouting, vegetative, booting and generative stages of rice development. In contrast, the promoter activity was present in anthers and aleurone layers of immature seeds 7–20 days after anthesis. Moreover, there was no promoter activity observed in the aleurone layers of mature seeds. The OsAER1 promoter activity is induced by Al-toxicity, NaCl and submergence stresses, indicating the OsAER1 promoter activity is induced by those stresses. Exogenous treatments of transgenic plants carrying the PA-5 promoter construct with abscisic acid and indoleacetic acid also induced expression of the GUS reporter transgene, indicating the role of plant growth regulators in controlling OsAER1 promoter activity. Promoter deletion analysis was conducted to identify the cis-acting elements of the promoter responsible for controlling root-specific expression. The GUS reporter gene was fused with various deletion fragments of the OsAER1 promoter and the resulting constructs were transformed in rice plants to generate transgenic plants. The results of this analysis indicated that cis-acting elements controlling root-specific expression are located between −1562 to −1026bp of the OsAER1 CDS. Here we discusses the results of the conducted analyses, the possible role of OsAER1 in rice growth and development, possible contributions and the potential usage of these findings in future plant research.

Endosperm specific expression of a gliadin-actin hybrid promoter in transgenic rice (Oryza sativa L.)

1999 ◽  
Vol 27 (3) ◽  
pp. 241-249 ◽  
Author(s):  
Ildikó Vibók ◽  
Tibor Nagy ◽  
Pedro Bittencourt ◽  
Barnabás Jenes ◽  
Géza Dallmann
Keyword(s):  
Oryza Sativa ◽  
Transgenic Rice ◽  
Oryza Sativa L ◽  
Specific Expression ◽  
Hybrid Promoter
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