Endosperm specific expression of a gliadin-actin hybrid promoter in transgenic rice (Oryza sativa L.)

1999 ◽  
Vol 27 (3) ◽  
pp. 241-249 ◽  
Author(s):  
Ildikó Vibók ◽  
Tibor Nagy ◽  
Pedro Bittencourt ◽  
Barnabás Jenes ◽  
Géza Dallmann
Keyword(s):  
Oryza Sativa ◽  
Transgenic Rice ◽  
Oryza Sativa L ◽  
Specific Expression ◽  
Hybrid Promoter

scholarly journals Senescence-Specific Expression of RAmy1A Accelerates Non-structural Carbohydrate Remobilization and Grain Filling in Rice (Oryza sativa L.)

2021 ◽  
Vol 12 ◽  
Author(s):  
Ning Ouyang ◽  
Xuewu Sun ◽  
Yanning Tan ◽  
Zhizhong Sun ◽  
Dong Yu ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Transgenic Rice ◽  
Oryza Sativa L ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Grain Filling ◽  
Leaf Sheath ◽  
Specific Expression ◽  
Total Soluble Sugar ◽  
Transgenic Lines

Remobilization of pre-anthesis NSCs (non-structural carbohydrates) is significant for effective grain filling in rice (Oryza sativa L.). However, abundant starch particles as an important component of NSCs are still present in the leaf sheath and stem at the late stage of grain filling. There are no studies on how bioengineering techniques can be used to improve the efficiency of NSC remobilization. In this study, RAmy1A was expressed under the senescence-specific promoter of SAG12, which was designed to degrade starch in the leaf sheath and stem during grain filling. RAmy1A mRNA successfully accumulated in the leaf, stem, and sheath of transgenic plants after anthesis. At the same time, the starch and total soluble sugar content in the leaf, stem, and leaf sheath were obviously decreased during the grain-filling period. The photosynthetic rate of transgenic lines was higher than that of the wild types by an average of 4.0 and 9.9%, at 5 and 10 days after flowering, respectively. In addition, the grain-filling rate of transgenic lines was faster than that of the wild types by an average of 26.09%. These results indicate an enhanced transport efficiency of NSCs from source tissues in transgenic rice. Transgenic rice also displayed accelerated leaf senescence, which was hypothesized to contribute to decreased grain weight.

State of the foreign gene and of the genome in transgenic rice (Oryza sativa L.)

1993 ◽  
Vol 11 (S1) ◽  
pp. S123-S125 ◽  
Author(s):  
P. H. Bao ◽  
S. Castiglione ◽  
C. Giordani ◽  
W. Li ◽  
G. Wang ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Transgenic Rice ◽  
Oryza Sativa L ◽  
Foreign Gene

A novel fungal arsenic methyltransferase, WaarsM reduces grain arsenic accumulation in transgenic rice (Oryza sativa L.)

2018 ◽  
Vol 344 ◽  
pp. 626-634 ◽  
Author(s):  
Shikha Verma ◽  
Pankaj Kumar Verma ◽  
Alok Kumar Meher ◽  
Amit Kumar Bansiwal ◽  
Rudra Deo Tripathi ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Transgenic Rice ◽  
Oryza Sativa L ◽  
Arsenic Accumulation

A TFIIIA-type zinc finger protein confers multiple abiotic stress tolerances in transgenic rice (Oryza sativa L.)

2012 ◽  
Vol 80 (3) ◽  
pp. 337-350 ◽  
Author(s):  
Ji Huang ◽  
Shujing Sun ◽  
Dongqing Xu ◽  
Hongxia Lan ◽  
Hui Sun ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Abiotic Stress ◽  
Zinc Finger ◽  
Transgenic Rice ◽  
Zinc Finger Protein ◽  
Oryza Sativa L ◽  
Finger Protein

Overexpression of OsRLCK241 confers enhanced salt and drought tolerance in transgenic rice (Oryza sativa L.)

Gene ◽  
2021 ◽  
Vol 768 ◽  
pp. 145278
Author(s):  
Hui Zhang ◽  
Niu Zhai ◽  
Xiang Ma ◽  
Huina Zhou ◽  
Yanchun Cui ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Drought Tolerance ◽  
Transgenic Rice ◽  
Oryza Sativa L ◽  
Salt And Drought Tolerance

scholarly journals High throughput metabolome and proteome analysis of transgenic rice plants (Oryza sativa L.)

2005 ◽  
Vol 22 (1) ◽  
pp. 47-50 ◽  
Author(s):  
Hideyuki Takahashi ◽  
Yuji Hotta ◽  
Mitsunori Hayashi ◽  
Maki Kawai-Yamada ◽  
Setsuko Komatsu ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Transgenic Rice ◽  
High Throughput ◽  
Oryza Sativa L ◽  
Proteome Analysis ◽  
Rice Plants ◽  
Transgenic Rice Plants

Analysis of a promoter for the NADH - glutamate synthase gene in rice (Oryza sativa): cell type-specific expression in developing organs of transgenic rice plants

2000 ◽  
Vol 27 (9) ◽  
pp. 787 ◽  
Author(s):  
Soichi Kojima ◽  
Michiko Kimura ◽  
Yukine Nozaki ◽  
Tomoyuki Yamaya
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Glutamate Synthase ◽  
Gus Activity ◽  
Start Codon ◽  
Assimilate Transport ◽  
Upstream Region ◽  
Vascular Bundles ◽  
Specific Expression ◽  
Gus Reporter

This paper originates from a presentation at the International Conference on Assimilate Transport and Partitioning, Newcastle, NSW, August 1999 The entire 3.7 kbp 5´-upstream region (–2840 to +886) from the translational start codon of NADH–glutamate synthase (NADH–GOGAT, EC 1.4.1.14) gene from rice (Oryza sativa L.) or the region sequentially deleted from the 5´-end was fused with the β−glucuronidase (GUS) reporter gene. The chimeric gene was introduced into calli derived from rice scutellum via Agrobacterium tumefaciens-mediated transformation and tissue-specific GUS activity determined in T0 generations. When the entire region was fused, GUS activity was detected in vascular bundles of the developing leaf blade and in dorsal and lateral vascular bundles of developing grains. This corresponds with our previous immunodetection of NADH–GOGAT protein (Hayakawa et al., Planta 193, 455–460, 1994). A series of deletion experiments showed that a 149-nucleotide region between –142 and +7 was essential for promoter activity in the NADH–GOGAT gene.

Sign in / Sign up

Export Citation Format

Share Document