Direct Gene Transfer to Protoplasts

1990 ◽  
Vol 17 (3) ◽  
pp. 291 ◽  
Author(s):  
PJ Larkin ◽  
BH Taylor ◽  
M Gersmann ◽  
RIS Brettell
Keyword(s):  
Polyethylene Glycol ◽  
Nicotiana Tabacum ◽  
Gene Transfer ◽  
Suspension Culture ◽  
Medicago Sativa ◽  
Transient Expression ◽  
Nicotiana Plumbaginifolia ◽  
Direct Gene Transfer ◽  
Direct Transfer ◽  
Direct Gene

Direct transfer of genes to protoplasts is considered, using electroporation and polyethylene-glycol-induced plasmid uptake. Both transient expression and stable transformation are discussed, with data and comparisons from the following protoplast systems: Nicotiana plumbaginifolia suspension culture, Nicotiana tabacum leaf, Medicago sativa leaf and Triticum monococcum suspension culture.

Polyethylene glycol-mediated direct gene transfer in Nicotiana spp.

1992 ◽  
Vol 85 (2) ◽  
pp. 345-351 ◽  
Author(s):  
Gokarna B. Gharti-Chhetri ◽  
Wichai Cherdshewasart ◽  
Jocelyne Dewulf ◽  
Michel Jacobs ◽  
loan Negrutiu
Keyword(s):  
Polyethylene Glycol ◽  
Gene Transfer ◽  
Direct Gene Transfer ◽  
Direct Gene

Direct gene transfer via polyethylene glycol

1989 ◽  
Vol 12 (4) ◽  
pp. 127-133 ◽  
Author(s):  
C. M. Johnson ◽  
G. K. Carswell ◽  
R. D. Shillito
Keyword(s):  
Polyethylene Glycol ◽  
Gene Transfer ◽  
Direct Gene Transfer ◽  
Direct Gene
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