Fusion of Agrobacterium and E. coli spheroplasts with Nicotiana tabacum protoplasts ? Direct gene transfer from microorganism to higher plant

1984 ◽  
Vol 3 (2) ◽  
pp. 60-64 ◽  
Author(s):  
R. Hain ◽  
H.-H. Steinbi� ◽  
J. Schell
Keyword(s):  
Nicotiana Tabacum ◽  
Gene Transfer ◽  
Direct Gene Transfer ◽  
Higher Plant ◽  
E Coli ◽  
Direct Gene

Direct Gene Transfer to Protoplasts

1990 ◽  
Vol 17 (3) ◽  
pp. 291 ◽  
Author(s):  
PJ Larkin ◽  
BH Taylor ◽  
M Gersmann ◽  
RIS Brettell
Keyword(s):  
Polyethylene Glycol ◽  
Nicotiana Tabacum ◽  
Gene Transfer ◽  
Suspension Culture ◽  
Medicago Sativa ◽  
Transient Expression ◽  
Nicotiana Plumbaginifolia ◽  
Direct Gene Transfer ◽  
Direct Transfer ◽  
Direct Gene

Direct transfer of genes to protoplasts is considered, using electroporation and polyethylene-glycol-induced plasmid uptake. Both transient expression and stable transformation are discussed, with data and comparisons from the following protoplast systems: Nicotiana plumbaginifolia suspension culture, Nicotiana tabacum leaf, Medicago sativa leaf and Triticum monococcum suspension culture.

Polyethylene glycol-mediated direct gene transfer in Nicotiana spp.

1992 ◽  
Vol 85 (2) ◽  
pp. 345-351 ◽  
Author(s):  
Gokarna B. Gharti-Chhetri ◽  
Wichai Cherdshewasart ◽  
Jocelyne Dewulf ◽  
Michel Jacobs ◽  
loan Negrutiu
Keyword(s):  
Polyethylene Glycol ◽  
Gene Transfer ◽  
Direct Gene Transfer ◽  
Direct Gene

Direct Gene Transfer: Basic Studies and Human Therapies

1993 ◽  
Vol 70 (01) ◽  
pp. 202-203 ◽  
Author(s):  
Elizabeth G Nabel ◽  
Gary J Nabel
Keyword(s):  
Gene Transfer ◽  
Direct Gene Transfer ◽  
Direct Gene ◽  
Basic Studies
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