Free Amino Acid Concentrations in Leaves of Sodium-Deficient C4 Plants

1986 ◽  
Vol 13 (3) ◽  
pp. 343 ◽  
Author(s):  
CPL Grof ◽  
M Johnston ◽  
PF Brownell

Consistent changes in the free amino acid concentrations in response to sodium nutrition were observed in mature leaves of the C4 species Amaranthus tricolor and Chloris gayana. The amino acids alanine, γ-aminobutyric acid and glycine were present in greater and aspartate and arginine in lower concentrations in mature leaves of sodium-deficient than in normal plants of both species.

1987 ◽  
Vol 17 (1) ◽  
pp. 27-30 ◽  
Author(s):  
Y. T. Kim ◽  
C. Glerum ◽  
J. Stoddart ◽  
S. J. Colombo

Greenhouse-grown black spruce and jack pine container seedlings were fertilized weekly with a 20–8–20 fertilizer at four concentrations during the fall. Seedlings were sampled when 23 weeks old towards the end of the greenhouse cultural period to determine the effect of fertilization on the free amino acid concentrations. All amino acids, except tryptophan, showed significant increases in concentration with higher levels of fertilizer; the concentration of tryptophan decreased with increasing fertilizer concentration. Amino acids with the highest concentrations in black spruce were arginine, glutamic acid, and proline, while in jack pine, besides these three, aspartic acid and glutamine were also found in high concentrations. Black spruce had significantly higher amino acid concentrations than jack pine. The concentrations of certain free amino acids may be more sensitive indicators of seedling nitrogen status than total foliar nitrogen.


1973 ◽  
Vol 59 (1) ◽  
pp. 57-63 ◽  
Author(s):  
W. DE LOECKER ◽  
M. L. STAS

SUMMARY Changes in the concentrations of free amino acids in intracellular fluids and blood plasma were measured in rats treated with cortisol. Increasing age raised the concentrations of free amino acids in plasma, while in liver, with the exception of glycine and alanine, decreased concentrations were observed. Cortisol treatment reduced free amino acid levels in plasma and liver which suggested a progressive catabolism of body proteins and increased protein synthesis in the liver. In skeletal muscle of control rats the free amino acid concentrations increased during the experimental period. Cortisol increased the concentration of certain amino acids and decreased that of others due to an increased protein turnover in muscle.


1991 ◽  
Vol 71 (3) ◽  
pp. 953-957 ◽  
Author(s):  
G. W. Sedgwick ◽  
T. W. Fenton ◽  
J. R. Thompson

The effect of the protein precipitants acetone, acetonitrile, perchloric acid and trichloroacetic acid on free amino acid concentrations in supernatants from ovine plasma and bovine serum albumin solutions was determined. The organic precipitants decreased (P < 0.05) free amino acid concentrations in a protein concentration dependent manner while the acid precipitants had no effect. Key words: Amino acids, protein precipitating agents


1974 ◽  
Vol 61 (2) ◽  
pp. 463-472
Author(s):  
P. D. EVANS ◽  
A. C. CROSSLEY

Free amino acid concentrations have been measured in haemolymph samples taken from 3rd instar larvae of the blowfly, Calliphora vicina, at various stages prior to pupariation. The amino acids found in the haemocyte fraction only accounted for 6% of the total free amino acid concentration of the haemolymph. However, a high percentage of the dicarboxylic amino acids, glutamate and aspartate, 62% and 69% respectively, appeared to be sequestered in the haemocyte fraction at 72 h prior to pupariation. The percentage of the other individual amino acids found in the haemocyte fraction represented less than 10% of their amount in whole haemolymph. It is proposed that these results, together with the increase in the haemocyte levels of glutamate observed after injecting larvae with saline containing glutamate, are one of the first indications of a homeostatic function of insect haemocytes with respect to haemolypmh amino acids.


1990 ◽  
Vol 20 (8) ◽  
pp. 1132-1136 ◽  
Author(s):  
Ann-Britt Edfast ◽  
Torgny Näsholm ◽  
Anders Ericsson

The effects of nitrogen deposition and site quality on amino acid concentrations in needles of Piceaabies (L.) Karst. and Pinussylvestris L. trees were studied in two areas that represent different levels of nitrogen deposition: one area in southern Sweden and one area in northern Sweden receive, respectively, approximately 20–30 and 3 kg•ha−1•year−1 of nitrogen. On each area three site quality classes were chosen for each tree species. The site classes were chosen to represent poor, medium, and good sites typical for each area. Free amino acids in the needles were analyzed as 9-fluorenylmethyl formate derivatives by high-performance liquid chromatography. The total nitrogen was determined with a CHN elemental analyzer, and other mineral nutrients were determined with an inductively coupled plasma analyzer. Arginine, glutamic acid, glutamine, γ-aminobutyric acid, and aspartic acid were the quantitatively dominating amino acids in the needles of both species from all sites in both northern and southern Sweden. These amino acids represented 50–80% of the total concentration of free amino acids in the needles. The concentration of arginine in the needles of both spruce and pine increased with decreasing site index and showed high variations between individual trees. For both species, the highest concentrations of arginine were found in the southern area, which had the highest deposition of nitrogen. Concentrations of glutamic acid, glutamine, and γ-aminobutyric acid in the needles of both species showed significant differences between some of the sites on both areas, but these differences showed no general pattern that correlated with the site indexes. In relation to nitrogen, low concentrations of phosphorus and potassium were found in needles from the poorest spruce sites in both areas compared with corresponding values for the good spruce sites. The results are discussed in relation to nitrogen deposition and mineral nutrient imbalance.


1976 ◽  
Vol 54 (11) ◽  
pp. 1927-1931 ◽  
Author(s):  
J. Alison Hanson ◽  
Thomas H. Dietz

Free amino acids constitute 11% of the cellular solute in Ligumia subrostrata acclimated in pond water but less than 1% of blood total solute. Arginine, glutamate, histidine, and alanine account for 60% of the cellular free amino acid concentration. Tissue free amino acid concentrations increase when the animals are exposed to dehydration and anoxic stress. Free amino acids contributing most to the elevated tissue amino acid concentrations are glutamate, alanine, threonine, and serine. These specific free amino acids play an important role in cellular volume regulation.


1988 ◽  
Vol 18 (10) ◽  
pp. 1286-1290 ◽  
Author(s):  
Y. T. Kim ◽  
C. Glerum

Seasonal free amino acid concentrations in current-year red pine (Pinusresinosa Ait.) needles were determined weekly or biweekly from September to December over a 3-year period. Tryptophan and histidine were the only amino acids that had significant positive trends, increasing in concentration between September and December in all 3 years, while threonine had a significant negative trend, decreasing in concentration in all 3 years. The positive tryptophan trend was the most consistent.


2013 ◽  
Vol 57 (1) ◽  
pp. 47-52 ◽  
Author(s):  
Adam Brodzki ◽  
Piotr Brodzki ◽  
Maria Szpetnar ◽  
Marcin R. Tatara

Abstract The aim of the study was to determine serum free amino acid concentration in male dogs suffering from benign and malignant perianal tumours. Serum concentrations of cysteic acid, glutamic acid, glutamine, aspartic acid, glycine, γ-aminobutyric acid, tryptophan, methionine, arginine, taurine, threonine, tyrosine, alanine, valine, isoleucine, and leucine were significantly changed in dogs suffering from benign and/or malignant tumours when compared to the control group (P<0.05). Serum concentration of serine, phenylalanine, lysine and histidine was not influenced in dogs with neoplastic disease (P>0.05). The evaluation of serum free amino acid concentration, has shown that threonine, glutamic acid, alanine, valine, isoleucine, leucine, γ-aminobutyric acid, and glycine have the highest diagnostic and prognostic value in dogs suffering from benign and malignant perianal tumours. Highly diagnostic and prognostic value in relation to benign tumour growth was also found while evaluating branched chain and acidic groups of amino acids. Serine, phenylalanine, lysine, and histidine, as well as alkaline amino acids were proved not to have diagnostic and prognostic value in dogs with the tumours. Thus, the evaluation of free amino acid concentration may serve diagnostic purposes and help in tumour malignancy differentiation. The elaborated experimental model may be used in further studies on neoplastic disease development and monitoring of applied treatment efficiency.


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