Effect of Brassinolide and other Growth Regulators on the Germination and Growth of Pollen Tubes of Prunus avium using a Multiple Hanging-drop Assay

1985 ◽  
Vol 12 (2) ◽  
pp. 201 ◽  
Author(s):  
FR Hewitt ◽  
T Hough ◽  
P O'Neill ◽  
JM Sasse ◽  
EG Williams ◽  
...  

A new multiple hanging-drop assay has been tested with a growth inhibitor, cycloheximide, and a growth-promoter, fusicoccin. This assay has been used to examine the growth-response of pollen-tubes of Prunus avium to brassinolide, a naturally occurring promoter of plant growth. This response is compared with that of indol-3-ylacetic acid, gibberellic acid, and kinetin. Pollen tubes responded to brassinolide and fusicoccin at 1 nM and above, a concentration one order of magnitude lower than that for indol-3-ylacetic acid and gibberellic acid. Kinetin did not stimulate growth of the pollen tubes.

2005 ◽  
Vol 60 (1-2) ◽  
pp. 72-78 ◽  
Author(s):  
Henriete S. Vieira ◽  
Jacqueline A. Takahashi ◽  
Lúcia P. S. Pimenta ◽  
Maria Amélia D. Boaventura

Kaurenoic and grandiflorenic acid, isolated from Wedelia paludosa (Asteraceae), some derivatives from these acids (alcohols, esters, amides, lactones, oximes) and other naturally occurring kaurane diterpenes were tested for their action on the growth of radical and shoot of Lactuca sativa. Gibberellic acid, GA3, a commercially available phytohormone, belonging to the same class of diterpenes, was also tested. Some of the tested substances showed a remarkable activity either in the inhibition or in stimulation of L. sativa growth. The activity, in some cases, was even higher than that of GA3.


1961 ◽  
Vol 41 (4) ◽  
pp. 740-743 ◽  
Author(s):  
G. W. Eaton

Effects of fungicidal concentrations of sulphur, dichlone, ferbam, and captan on sweet cherry (Prunus avium L.) pollen germination and growth were studied in artificial media. Sulphur did not reduce the germination of pollen. Dichlone and ferbam reduced germination from 53.0 per cent to 47.1 per cent and 40 per cent, respectively, reductions which were significant statistically. Sprayed at 0.2 pound/100 gallons or less, captan did not reduce germination. Captan, sprayed at 2 pounds/100 gallons, almost entirely prevented pollen germination, and arrested the elongation of pollen tubes.


2007 ◽  
Vol 87 (3) ◽  
pp. 545-550 ◽  
Author(s):  
J. A. Cline ◽  
M. Trought

Rain-induced cracking of sweet cherries (Prunus avium L.) is a major problem in many cherry growing regions throughout the world. One method to reduce cracking is to grow cracking resistant cultivars; however, no completely effective control measures are currently available to eliminate this problem. Studies conducted in New Zealand to test the ameliorating properties of gibberellic acid sprays to reduce cracking were examined. The results revealed that repeated or singular foliar applications at 10 or 40 mg L-1 gibberellic acid (GA3), respectively, increased both fruit cracking, and fruit firmness, but delayed fruit colour development. Key words: Prunus avium, cherry splitting, cracking


2018 ◽  
Vol 115 (20) ◽  
pp. 5072-5076 ◽  
Author(s):  
Christian Schröter ◽  
Jong Chan Lee ◽  
Thomas Schultz

We present mass-correlated rotational alignment spectroscopy, based on the optical excitation of a coherent rotational quantum wave and the observation of temporal wave interferences in a mass spectrometer. Combined electronic and opto-mechanical delays increased the observation time and energy resolution by an order of magnitude compared with preceding time-domain measurements. Rotational transition frequencies were referenced to an external clock for accurate absolute frequency measurements. Rotational Raman spectra for six naturally occurring carbon disulfide isotopologues were resolved with 3 MHz resolution over a spectral range of 500 GHz. Rotational constants were determined with single-kilohertz accuracy, competitive with state-of-the-art frequency domain measurements.


2011 ◽  
Vol 33 (No. 3) ◽  
pp. 119-123 ◽  
Author(s):  
M. Çetinbaş ◽  
F. Koyuncu

To break dormancy and increase the germination of Prunus avium L. (mazzard cherry) seeds, various methods were tested including the removal of the seed coat after cold moist stratification and treatment with GA<sub>3</sub>, KNO<sub>3</sub>, or thiourea. Treatments with 7,500 ppm KNO<sub>3</sub> after 120 days of stratification were more effective, yielding 64.54% germination of seeds with coat. In seeds without coat, 500 ppm GA<sub>3</sub> treatment after 120 days of stratification gave 79.74% germination; a value increased about 29% compared to control. &nbsp;


1996 ◽  
Vol 36 (2) ◽  
pp. 237 ◽  
Author(s):  
OP Damasco ◽  
ID Godwin ◽  
MK Smith ◽  
SW Adkins

Detection of dwarf offtypes produced by micropropagation of Cavendish bananas (Musa spp.) cultivars New Guinea Cavendish and Williams was achieved by spraying gibberellic acid (GA3) solution (289 pmol/L) onto deflasked plants and measuring various plantlet responses. The most useful identification criterion was elongation of the sheath of the first leaf to form after GA3 application. Elongation of this structure was about 2-fold greater in normal plants than observed in dwarfs. Similar measurements taken earlier during in vitro culture or later during plant establishment in soil were not as useful in discriminating between normals and dwarfs as the measurements made at deflasking. The similar GA3-induced elongation response of the dwarf offtype and that of the naturally occurring dwarf cultivar Dwarf Parfitt suggests that the mechanism for dwarfism could be the same in the 2 cases.


2003 ◽  
Vol 30 (9) ◽  
pp. 995 ◽  
Author(s):  
Hisashi Kato-Noguchi ◽  
Takeshi Ino ◽  
Masahiko Ichii

Momilactone B was released into the neighboring environment from rice throughout its life cycle. The rate of momilactone B release from rice increased until flowering initiation, and then decreased. The release rate of momilactone B at the day of flowering started was 2.1 μg plant–1 d–1. On average, a single rice plant released about 100 μg of momilactone B into the neighboring environment over its life cycle. Since momilactone B is a growth inhibitor, these results suggest that momilactone B released from rice plants may serve as an allelochemical to inhibit the germination and growth of neighboring plants.


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