Molecular cloning and expression of a cDNA encoding a microsomal w-6 fatty acid desaturase from cotton (Gossypium hirsutum)

1999 ◽  
Vol 26 (2) ◽  
pp. 101 ◽  
Author(s):  
Qing Liu ◽  
Surinder P. Singh ◽  
Curt L. Brubaker ◽  
Peter J. Sharp ◽  
Allan G. Green ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Blot Analysis ◽  
Northern Blot Analysis ◽  
Fatty Acid Desaturase ◽  
Cloning And Expression ◽  
Tetraploid Cotton ◽  
Coding Region ◽  
Gossypium Hirsutum L ◽  
Cotton Species ◽  
Diploid Cotton

A cDNA (ghFAD2-1) encoding a seed-specific microsomal ω-6 desaturase was isolated from a cotton (Gossypium hirsutum L. cv. Deltapine-16) embryo cDNA library. The deduced amino acid sequence showed substantial similarity to other plant microsomal ω-6 desaturases. Northern blot analysis indicated that the ghFAD2-1 transcript was specifically induced during embryo development and expression of the transcript could not be detected in leaves. Southern blot analysis using the coding region and 3′ untranslated region of ghFAD2-1 revealed that microsomal ω-6 desaturase in cotton is encoded by a small multigene family. There are at least two copies of ghFAD2-1 in two tetraploid cotton species (G. hirsutum L. and G. barbadense L.) and at least one copy in diploid cotton species (G. herbaceum L., G. raimondii Ulbrich and G. robinsonii Mueller).

Molecular mapping of a new source of Fusarium wilt resistance in tetraploid cotton (Gossypium hirsutum L.)

2012 ◽  
Vol 30 (2) ◽  
pp. 1181-1191 ◽  
Author(s):  
L. Augusto Becerra Lopez-Lavalle ◽  
Vanessa J. Gillespie ◽  
Walter A. Tate ◽  
Marc H. Ellis ◽  
Warwick N. Stiller ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Fusarium Wilt ◽  
Molecular Mapping ◽  
Tetraploid Cotton ◽  
Gossypium Hirsutum L ◽  
Wilt Resistance ◽  
Fusarium Wilt Resistance

scholarly journals Genome Physical Mapping of Polyploids: A BIBAC Physical Map of Cultivated Tetraploid Cotton, Gossypium hirsutum L

PLoS ONE ◽  
2012 ◽  
Vol 7 (3) ◽  
pp. e33644 ◽  
Author(s):  
Meiping Zhang ◽  
Yang Zhang ◽  
James J. Huang ◽  
Xiaojun Zhang ◽  
Mi-Kyung Lee ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Physical Mapping ◽  
Physical Map ◽  
Tetraploid Cotton ◽  
Gossypium Hirsutum L

scholarly journals Morphological description of a novel synthetic allotetraploid(A1A1G3G3) of Gossypium herbaceum L.and G.nelsonii Fryx. suitable for disease-resistant breeding applications

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0242620
Author(s):  
Xiaomin Yin ◽  
Rulin Zhan ◽  
Yingdui He ◽  
Shun Song ◽  
Lixia Wang ◽  
...  
Keyword(s):  
Chromosome Doubling ◽  
Diploid Species ◽  
Morphological Description ◽  
Tetraploid Cotton ◽  
Distant Hybridization ◽  
Cotton Species ◽  
Cotton Plants ◽  
Wild Cotton ◽  
Diploid Cotton ◽  
Gossypium Herbaceum

Wild species of Gossypium ssp. are an important source of traits for improving commercial cotton cultivars. Previous reports show that Gossypium herbaceum L. and Gossypium nelsonii Fryx. have better disease resistance characteristics than commercial cotton varieties. However, chromosome ploidy and biological isolation make it difficult to hybridize diploid species with the tetraploid Gossypium hirsutum L. We developed a new allotetraploid cotton genotype (A1A1G3G3) using a process of distant hybridization within wild cotton species to create new germplasms. First of all, G. herbaceum and G. nelsonii were used for interspecific hybridization to obtain F1 generation. Afterwards, apical meristems of the F1 diploid cotton plants were treated with colchicine to induce chromosome doubling. The new interspecific F1 hybrid and S1 cotton plants originated from chromosome duplication, were tested via morphological and molecular markers and confirmed their tetraploidy through flowrometric and cytological identification. The S1 tetraploid cotton plants was crossed with a TM-1 line and fertile hybrid offspring were obtained. These S2 offsprings were tested for resistance to Verticillium wilt and demonstrated adequate tolerance to this fungi. The results shows that the new S1 cotton line could be used as parental material for hybridization with G. hirsutum to produce pathogen-resistant cotton hybrids. This new S1 allotetraploid genotype will contributes to the enrichment of Gossypium germplasm resources and is expected to be valuable in polyploidy evolutionary studies.

Analysis of genetic diversity, population structure and linkage disequilibrium in elite cotton (Gossypium L.) germplasm in India

2011 ◽  
Vol 62 (10) ◽  
pp. 859 ◽  
Author(s):  
Satya Narayan Jena ◽  
Anukool Srivastava ◽  
Uma Maheswar Singh ◽  
Sribash Roy ◽  
Nandita Banerjee ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Linkage Disequilibrium ◽  
Diploid Species ◽  
Aflp Markers ◽  
Tetraploid Cotton ◽  
Cotton Species ◽  
Geographical Regions ◽  
Wide Range ◽  
Diploid Cotton ◽  
Efficient Breeding

An understanding of the level of genetic diversity is a prerequisite for designing efficient breeding programs. Fifty-one cultivars of four cotton species (Gossypium hirsutum, G. barbadense, G. herbaceum and G. arboreum) representing core collections at four major cotton research stations with a wide range of eco-geographical regions in India were examined for the level of genetic diversity, distinct subpopulations and the level of linkage disequilibrium (LD) using 1100 amplified fragment length polymorphism (AFLP) markers with 16 primer pairs combinations. The AFLP markers enabled a reliable assessment of inter- and intra-specific genetic variability with a heterogeneous genetic structure. Higher genetic diversity was noticed in G. herbaceum, followed by G. arboreum. The genetic diversity in tetraploid cotton species was found to be less than that in the diploid species. The genotypes VAGAD, RAHS14, IPS187, 221 557, Jayhellar of G. herbaceum and 551, DLSA17, 221 566 of G. arboreum were identified as the most diverse parents, useful for quantitative trait loci (QTL) analysis in diploid cotton. Similarly, LRA 5166, AS3 and MCU5 of G. hirsutum and B1, B3, Suvin of G. barbadense were most diverse to develop mapping populations for fibre quality. The internal transcribed spacer sequences were sufficient to resolve different species and subspecies of diploid cotton. Low level of genome-wide LD was detected in the entire collection (r2 = 0.07) as well as within the four species (r2 = 0.11–0.15). A strong agreement was noticed between the clusters constructed on the basis of morphological and genotyping data.

scholarly journals RAPD and Cytogenetic Study of Some Tetraploid Cotton (Gossypium hirsutum L) Cultivars and Their Hybrids

CYTOLOGIA ◽  
2007 ◽  
Vol 72 (1) ◽  
pp. 77-82 ◽  
Author(s):  
Masoud Sheidai ◽  
Zahra H. Shahriari ◽  
H. Rokneizadeh ◽  
Zahra Noormohammadi
Keyword(s):  
Gossypium Hirsutum ◽  
Cytogenetic Study ◽  
Tetraploid Cotton ◽  
Gossypium Hirsutum L

A comparison of genetic maps constructed from haploid and BC1 mapping populations from the same crossing between Gossypium hirsutum L. and Gossypium barbadense L.

Genome ◽  
2005 ◽  
Vol 48 (3) ◽  
pp. 378-390 ◽  
Author(s):  
Xianliang Song ◽  
Kai Wang ◽  
Wangzhen Guo ◽  
Jun Zhang ◽  
Tianzhen Zhang
Keyword(s):  
Gossypium Hirsutum ◽  
Average Distance ◽  
Gossypium Barbadense ◽  
Genetic Maps ◽  
Marker Genes ◽  
Tetraploid Cotton ◽  
Linkage Groups ◽  
Gossypium Hirsutum L ◽  
Morphological Marker ◽  
Map Comparison

Simple sequence repeat (SSR) genetic maps have been separately constructed based on doubled haploid (DH) and (or) haploid and BC1 populations from the same cross between Gossypium hirsutum L. 'TM-1' and Gossypium barbadense L. 'Hai7124'. The BC1 population was produced by pollinating individual plants of the 'TM-1' × 'Hai7124' F1 with 'TM-1', whereas the DH and (or) haploid population developed from the offspring of Vsg × ('TM-1' × 'Hai7124'). Vsg is a virescently marked semigamy line of Gossypium barbadense L. Pima. The BC1 map included 34 linkage groups with an average distance between markers of 9.80 cM (Kosambi, K) and covered 4331.2 cM (K) or approximately 78.7% of the tetraploid cotton genome constructed using 440 SSR and 2 morphological marker genes. Among them, 26 were assigned to 20 chromosomes, 7 to A or D subgenomes, and 1 was unassigned. The haploid map comprised 444 SSR markers mapped to 40 linkage groups with an average distance of 7.35 cM (K) between markers, covering 3262.9 cM (K) or approximately 60.0% of the tetraploid genome. Twenty-nine linkage groups were assigned to all 19 identified chromosomes, 10 to A or D subgenomes, and 1 was unassigned. Fairly good collinearity of marker order was observed along most of the chromosomes or linkage groups. Significant differences in recombination between maps was observed at the chromosomal and genomic level and possible reasons were discussed. Map comparison and combined data provided an essential basis for further mapping of interested genes and QTLs and for studies of diversity, population structure, and phylogeny in Gossypium species.Key words: cotton, SSR, comparative mapping, semigamy.

scholarly journals Genetic finger printing of cotton cultivars by ISSR molecular markers

Genetika ◽  
2018 ◽  
Vol 50 (2) ◽  
pp. 627-634 ◽  
Author(s):  
Farah Farahani ◽  
Masoud Sheidai ◽  
Fahimeh Koohdar
Keyword(s):  
Molecular Markers ◽  
Genetic Variability ◽  
Gossypium Hirsutum ◽  
Agronomic Traits ◽  
Genetic Erosion ◽  
Continuous Selection ◽  
Tetraploid Cotton ◽  
Genetic Affinity ◽  
Cotton Species ◽  
Issr Molecular Markers

Gossypium hirsutum is one of the main tetraploid cotton species that is cultivated throughout the world. Due to continuous selection of cotton cultivars for specific agronomic traits, the genetic variability within the cultivars decrease that lead to genetic erosion. To tackle the problem of reduced genetic variability, we should track all available genetic diversity within cotton germplasm and use them for inter-specific and intra-specific hybridization and produce new elite cotton cultivars. Therefore, the present study used ISSR molecular markers to illustrate genetic variability in 13 tetraploid cotton genotypes (Gossypium hirsutum L.) and to categorize these genotypes based on genetic affinity. 65 cotton plants were studied. The results identified private bands in the studied genotypes, while Network and STRUCTURE analyses of molecular data obtained grouped the genotypes with genetic affinity together. Some of the genotypes differed in their genetic content from the others; therefore, studying the genetic and agronomic variability within available cultivars is very important and produced data to broaden the gene pool for planning further hybridization in cotton.

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