Frost Injury in Wheat: Ice Formation and Injury in Leaves

1979 ◽  
Vol 6 (4) ◽  
pp. 513 ◽  
Author(s):  
H Marcellos ◽  
MJ Burke

Freezing of water in leaves of a number of wheat cultivars was studied using pulsed nuclear magnetic resonance (n.m.r.) spectroscopy; these data were coordinated with measurements of the temperature dependance of freezing injury, and used to analyse the freezing process and its effects. The freezing temperature which resulted in 50% loss of cell electrolytes on thawing. T50, varied from -7 to -12.1°C in the material studied. Hardening treatments decreased T50 by 1.8° in the least hardy cultivar, Kite, and 3.3° in the most hardy cultivar, Cheyenne. Despite the contrast in hardiness level, freezing curves determined by n.m.r. were similar, the fraction of water remaining unfrozen, LT/L0, at temperature T having the relationship LT/L0 = ΔTm/T + b, where LT, L0 are the amounts of liquid water per unit dry weight at T and 0�C, respectively, ΔTm is the melting point depression and b the liquid water that does not freeze. The freezing curves were similar because ΔTm varied little among hardy and less hardy samples. At their T50, the least hardy sample of Kite had lost 77.2% of its freezable water to extracellular ice at - 7°C, whereas the corresponding value for the hardy Cheyenne was 86.8% at its T50 of - 12.1°C. Greater hardiness in samples was closely associated with an ability to withstand greater amounts of freeze-induced dehydration, and not with alterations in °Tm.

1964 ◽  
Vol 42 (6) ◽  
pp. 637-649 ◽  
Author(s):  
D. Siminovitch ◽  
H. Therrien ◽  
F. Gfeller ◽  
B. Rheaume

Amino acids and other soluble α-amino compounds are liberated into a leaching medium from tissues of black locust bark, alfalfa, and wheat that are frozen to temperatures which are injurious to the tissues. The amounts liberated increase with lowering in freezing temperature and are proportional to the loss in vital capacity of the tissue. Insignificant amounts of amino acids are released by leaching of non-frozen tissue while a maximum is reached at freezing temperatures which are completely lethal. The amino acids liberated from frozen and thawed tissues must originate from the destruction of living cells by the freezing process. The determination of the concentration of amino acids in the medium in which the plant tissues are leached after freezing can be used therefore as a quantitative method for the estimation of the injury sustained in the freezing.The use of the ninhydrin reaction for the purpose of this determination is described and its application to the estimation of freezing injury and resistance in a variety of hardy and non-hardy tissues of alfalfa, wheat, and black locust tree bark is shown. The extension of this procedure to the determination of injury produced by toxic and other detrimental agents is indicated.


1990 ◽  
Vol 20 (3) ◽  
pp. 280-284 ◽  
Author(s):  
Jarmo K. Holopainen

The responses of young Scots pine seedlings to mechanical apical meristem damage before and after 4 nights exposure to minimum night temperatures of −2.2 and −6.7 °C in controlled environment growth chambers were compared with control seedlings that were subjected or not to apical meristem damage and exposed to a minimum night temperature of 12 °C. The feeding damage caused by Lygus bugs was simulated by piercing the apical meristem of young pine seedlings with a hypodermic syringe needle and injecting a small drop of distilled water into the wound. At −6.7 °C increased numbers of dead seedlings were found. The proportion of seedlings with multiple leaders greatly increased after piercing, and about half of the seedlings subjected to the apical meristem damage had multiple leaders. The proportion of seedlings with multiple leaders and the number of leader shoots per seedling did not differ among seedlings subjected to apical meristem damage before or after the frost exposure. Short and twisted primary needles occurred in the basal parts of the new shoots in the seedlings with multiple leaders. Seedlings with necrotic needles were most often found after exposure to the night temperature of −6.7 °C. Shoot dry weight and length were significantly lower in seedlings subjected to apical meristem damage after frost exposure than in seedlings subjected to apical meristem damage before frost exposure or to no frost exposure. The results suggest that an increased reduction in growth is to be expected if Lygus bug attacks occur on pine seedlings that already suffer from frost injury.


2001 ◽  
Vol 13 (3) ◽  
pp. 271-279 ◽  
Author(s):  
Brent J. Sinclair ◽  
Heidi Sjursen

Cold tolerance of the springtail Gomphiocephalus hodgsoni Carpenter (Collembola: Hypogastruridae) was studied at Cape Bird, Ross Island, Antarctica (77°13′S, 166°26′E). Microclimate temperatures indicate a highly seasonal thermal environment, with winter minima <–39°C. Snow cover significantly buffers both minimum temperatures and cooling rates. Gomphiocephalus hodgsoni survives low temperatures by avoiding freezing. Mean low group supercooling points (SCPs) ranged from –35.4°C in October to –28.3°C in January. The lowest SCP measured was –38.0°C. The high SCP group was very small, making up only 18% of the population in January. In October, G. hodgsoni had a very high glycerol content (>80 μg mg−1 dry weight), although this declined rapidly to low levels (c. 7–10 μg mg−1 dry weight) in January. Quantities of glucose and trehalose were low during October, but steadily increased throughout the summer. Haemolymph osmolality was exceptionally high (up to 1755 mOsm kg−1) at the end of November, but this rapidly declined to c. 500 mOsm kg−1 by late December. The presence of thermal hystersis proteins was indicated by both osmometry on haemolymph samples and recrystallization inhibition studies of springtail homogenates. There was a strong relationship between glycerol content and SCP, but the relationship between haemolymph osmolality, SCP and carbohydrates is uncertain.


2009 ◽  
Vol 40 (1) ◽  
pp. 33-44 ◽  
Author(s):  
Nils Granlund ◽  
Angela Lundberg ◽  
James Feiccabrino ◽  
David Gustafsson

Ground penetrating radar operated from helicopters or snowmobiles is used to determine snow water equivalent (SWE) for annual snowpacks from radar wave two-way travel time. However, presence of liquid water in a snowpack is known to decrease the radar wave velocity, which for a typical snowpack with 5% (by volume) liquid water can lead to an overestimation of SWE by about 20%. It would therefore be beneficial if radar measurements could also be used to determine snow wetness. Our approach is to use radar wave attenuation in the snowpack, which depends on electrical properties of snow (permittivity and conductivity) which in turn depend on snow wetness. The relationship between radar wave attenuation and these electrical properties can be derived theoretically, while the relationship between electrical permittivity and snow wetness follows a known empirical formula, which also includes snow density. Snow wetness can therefore be determined from radar wave attenuation if the relationship between electrical conductivity and snow wetness is also known. In a laboratory test, three sets of measurements were made on initially dry 1 m thick snowpacks. Snow wetness was controlled by stepwise addition of water between radar measurements, and a linear relationship between electrical conductivity and snow wetness was established.


2021 ◽  
Vol 905 (1) ◽  
pp. 012002
Author(s):  
C Prayogo ◽  
C Muthahar ◽  
R M Ishaq

Abstract The cause of global warming is the increasing carbon concentration arising from industrial activities, burning of fossils, and land-use change. The purpose of this research was to find out the allometric equation to calculate the local bamboo biomass and then to be able to calculate how much carbon sequestration at bamboo riparian forest since this area was rarely being explored. The parameters observed were the height and diameter of the bamboo stem at 1.3 m height of 6 types of local bamboo using destructive sampling, along with the measurement of bamboo weight. The carbon content of the bamboo biomass, litter, and soil was measured to complement the estimation of total carbon sequestration. The results showed that the allometric equation for estimating local bamboo biomass is Y=0.6396 X1.6162 with R2=0.77, obtained from the relationship equations between dry weight and the diameter. Total carbon sequestration of this system ranged between 81 to 215 tons C ha−1.


2016 ◽  
Vol 8 (1) ◽  
pp. 20 ◽  
Author(s):  
M. Bänziger ◽  
G. O. Edmeades ◽  
J. Bolaños

The amount of dry matter produced during various stages of corn growth is a important variable to be taken into consideration. However, the lack of drying facilities makes its measurement a difficult task in the fields. A simple method to convert the fresh weight of a crop in the field into dry weight, could be an answer to that problem. In this study, we calculated the relationship between fresh and dry weight of corn stovers, over several, growth, stages of eight corn cultivars of different vigour and maturity period, at two Mexican locations. The differences between cultivars were for percent stover dry weight (%SDW) most evident in the second half of the grain growth stage, when late cultivars showed less humidity than the early ones. The % SDW was regressed against the phenological developmental stage and expressed as a ratio against antesis (R, days to sampling /days to 50% antesis). The equations (R2 = 0.97 - 0.99) with best results were: Early maturing cultivars: %SDW = 12.6 + 0.94R2 + 1.68R4; Late: %SDW = 16.1 - 4.00 R2 + 3.36R4. There were no consistant differences among cultivars with different vigour levels, even though certain differences were noted among the locations and they were attributed to differences in relative humidity. We describe a protocol for determining the dry weight of corn stover by area unit (t/ha) when drying conditions are not available, by utilizing only a scale and a ruler.We also suggest a method to calculate percent dry matter for a real plant parts (including grain).


2012 ◽  
Vol 184-185 ◽  
pp. 818-825 ◽  
Author(s):  
Xiao Yong Zhang ◽  
Yan Zhang ◽  
Dou Zhang

Porous Al2O3 ceramics with a lamellar pore microstructure have been fabricated by a directional freezing process using the micro-sized and nano-sized Al2O3 powders. At the same solid loading and freezing temperature, compared with the suspensions of micro-sized Al2O3, the porous ceramics prepared from the suspensions of nano-sized Al2O3 had the narrower lamellar pore channels and thicker channel walls, which were caused by the higher viscosity, stronger inter-particle attraction and more ice nucleation sites in the nano-sized Al2O3 suspensions. For both types of Al2O3 at a specific solid loading, with the increasing of freezing temperature, the wider pore channels and thinner channel walls were observed. These tendencies were weaker by using the nano-sized Al2O3 . The above results show the lower porosity in the ceramics using the nano-sized Al2O3. However, due to the higher sintering activity for the smaller particles, the pore channel walls by using the nano-sized Al2O3 was denser than using the micro-sized Al2O3, leading to the higher mechanical strength of ceramics.


1984 ◽  
Vol 62 (9) ◽  
pp. 1781-1790 ◽  
Author(s):  
S. I. Warwick ◽  
B. K. Thompson ◽  
L. D. Black

Thirteen populations of Sorghum halepense, Johnson grass, were sampled from fields in Ontario, Canada, and Ohio and New York, United States. Only four of these populations were reported to overwinter as rhizomes. The morphology, phenology, resource allocation patterns, and growth of seedling and mature plants of the overwintering and the non-overwintering populations were compared. Field-collected specimens from the nonoverwintering populations had wider culms and leaves and larger seeds and inflorescences. Analysis of material grown in a 5-month greenhouse trial indicated similar differences. Greenhouse plants from the nonoverwintering populations were also characterized by greater percent emergence, larger and faster growing seedlings, earlier flowering, larger culms and seeds, greater reproductive dry weight per plant, and about 1/10th the rhizome dry weight of overwintering plants. Differences between populations within a biotype were evident for both biotypes, although there was little within-population variation, except in rhizome production, where certain individuals of some nonoverwintering populations did not produce extended rhizomes. Among the five enzymes which were examined electrophoretically, only one, phosphoglucomutase (PGM), showed variable isozyme patterns. No differences in enzyme patterns were apparent between the overwintering and the nonoverwintering biotypes. The relationship of the nonoverwintering populations to the cultivated species, Sorghum bicolor and S. almum, an introgressant between S. halepense and S. bicolor, is discussed.


1979 ◽  
Vol 59 (3) ◽  
pp. 585-601 ◽  
Author(s):  
G. O. EDMEADES ◽  
T. B. DAYNARD

In an attempt to explain plant-to-plant variation in dry weight of maize (Zea mays L.), a computer program was developed to predict daily assimilation per plant and its distribution throughout the shoot at flowering. Inputs to the model were meteorological data, photosynthetic rate-irradiance curves, measurements of intraplant assimilate distribution at flowering, and the positions of individual leaves of plants grown in the field at three densities (50 000, 100 000 and 150 000 plants/ha). Dry weights were recorded on these same plants following black layer formation. Predicted effects of plant density on shoot growth compared favorably with available data. The correlation coefficient between predicted assimilation 1 day after anthesis and grain yield on the same plants, with treatment effects removed, was 0.67 (N = 360). The coefficient of variation of predicted assimilate flux per plant increased significantly with increasing density, and the fluxes were generally normally distributed. Results supported the concept of a threshold assimilation rate per plant below which grain would not normally form, and this appears to be the cause of the bimodal frequency distribution of grain yield per plant observed at high densities.


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