Proteolysis in Cotyledon Cells of Phaseolus vulgaris L.: Changes in Multiple Hydrolase Activities Following Germination

1979 ◽  
Vol 6 (4) ◽  
pp. 467 ◽  
Author(s):  
JA Crump ◽  
DR Murray
Keyword(s):  
Acid Phosphatase ◽  
Phaseolus Vulgaris ◽  
Hydrolytic Enzymes ◽  
Maximum Activity ◽  
Hydrolase Activity ◽  
Phaseolus Vulgaris L ◽  
Maximum Value ◽  
Cotyledon Cells

Changes in activity of several hydrolytic enzymes were measured in extracts prepared from the cotyledons following germination of bean seeds (Phaseolus vulgaris cv. Hawkesbury Wonder). Aminopeptidase AP2 (phenanthroline-sensitive leucyl β-naphthylamidase), N-carbobenzoxy-L- tyrosine-p-nitrophenol ester (N-CBZ-L-Tyr-pNP) hydrolase, N-benzoyl-L-tyrosineethyl ester (BTEE) hydrolase and acid phosphatase all increased in activity per cotyledon, reaching maximum values after 4, 6, 10 and 10 days respectively. By comparison, aminopeptidase AP1 (phenanthrolineinsensitive leucyl β-naphthylamidase) showed maximum activity after 2 days and declined immediately. α-N-benzoyl-D,L-arginine-p-nitroanilide hydrolase activity was maintained at a maximum value between days 2 and 10 before declining. Differences in maximum activity per cotyledon, initial presence or absence, and timing of both increase and decrease of N-CBZ-L-Tyr-pNP and BTEE hydrolase activities suggest that at least two enzymes contribute to these activities, and that neither activity indicates the presence of a chymotrypsin-like endopeptidase.

scholarly journals Comparative Study between Exogenously Applied Plant Growth Hormones versus Metabolites of Microbial Endophytes as Plant Growth-Promoting for Phaseolus vulgaris L.

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1059
Author(s):  
Mohamed A. Ismail ◽  
Mohamed A. Amin ◽  
Ahmed M. Eid ◽  
Saad El-Din Hassan ◽  
Hany A. M. Mahgoub ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Plant Growth ◽  
Common Bean ◽  
Plant Biomass ◽  
Hydrolytic Enzymes ◽  
Molecular Techniques ◽  
Phaseolus Vulgaris L ◽  
Plant Growth Hormones ◽  
Growth Promoting ◽  
Brevibacillus Agri

Microbial endophytes organize symbiotic relationships with the host plant, and their excretions contain diverse plant beneficial matter such as phytohormones and bioactive compounds. In the present investigation, six bacterial and four fungal strains were isolated from the common bean (Phaseolus vulgaris L.) root plant, identified using molecular techniques, and their growth-promoting properties were reviewed. All microbial isolates showed varying activities to produce indole-3-acetic acid (IAA) and different hydrolytic enzymes such as amylase, cellulase, protease, pectinase, and xylanase. Six bacterial endophytic isolates displayed phosphate-solubilizing capacity and ammonia production. We conducted a field experiment to evaluate the promotion activity of the metabolites of the most potent endophytic bacterial (Bacillus thuringiensis PB2 and Brevibacillus agri PB5) and fungal (Alternaria sorghi PF2 and, Penicillium commune PF3) strains in comparison to two exogenously applied hormone, IAA, and benzyl adenine (BA), on the growth and biochemical characteristics of the P. vulgaris L. Interestingly, our investigations showed that bacterial and fungal endophytic metabolites surpassed the exogenously applied hormones in increasing the plant biomass, photosynthetic pigments, carbohydrate and protein contents, antioxidant enzyme activity, endogenous hormones and yield traits. Our findings illustrate that the endophyte Brevibacillus agri (PB5) provides high potential as a stimulator for the growth and productivity of common bean plants.

Effect of phosphorus deficiency on acid phosphatase and phytase activities in common bean (Phaseolus vulgaris L.) under symbiotic nitrogen fixation

Symbiosis ◽  
2009 ◽  
Vol 47 (3) ◽  
pp. 141-149 ◽  
Author(s):  
Saber Kouas ◽  
Julien Louche ◽  
Ahmed Debez ◽  
Claude Plassard ◽  
Jean Jacques Drevon ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Acid Phosphatase ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Symbiotic Nitrogen Fixation ◽  
Phosphorus Deficiency ◽  
Phaseolus Vulgaris L

ĐIỀU TRA, TINH SẠCH VÀ TÌM HIỂU TÍNH CHẤT ĐẶC TRƯNG CỦA LECTIN MỘT SỐ GIỐNG ĐẬU CÔ VE (PHASEOLUS VULGARIS L.)

2013 ◽  
Vol 75 (6) ◽  
Author(s):  
Cao Đăng Nguyên ◽  
Nguyễn Thị Cẩm Hạnh
Keyword(s):  
Phaseolus Vulgaris ◽  
Phaseolus Vulgaris L ◽  
White Bean

Đã điều tra lectin của 6 giống đậu cô ve thấy rằng cả 6 giống đều có hoạt tính lectin trong đó giống đậu cove hạt trắng dạng bụi (white bean core bush type white seeds) có hoạt tính lectin mạnh nhất, đặc biệt đối với hồng cầu trâu, bò, lợn. Lectin của 6 giống này đều không có biểu hiện đặc hiệu nhóm máu. Lectin đậu cove hạt trắng dạng bụi hoạt động tốt nhất ở nhiệt độ 300C – 400C, pH 6,8 – 7,6. Các đường α-D-glucose, α-D-galactose, D-mannose, D-fructose, D-saccharide,  D-lactose, D-arabinose và D-manitose ở nồng độ 0,05 – 0,1 M có tác dụng kìm hãm hoạt tính của lectin đậu cove hạt trắng dạng bụi. Lectin này cũng bị kìm hãm bởi protein của một số huyết thanh người và động vật (trâu, bò, lợn). Đã tinh sạch lectin đậu cove hạt trắng dạng bụi có độ tinh sạch gấp khoảng 52 lần so với dịch thô ban đầu. Trên gel polyacrylamide thấy xuất hiện 5 band có khối lượng phân tử trong khoảng 30 – 97 kDa.

Análisis de la variabilidad genética en poblaciones primitivas y silvestres de Phaseolus vulgaris, mediante marcadores bioquímicos y moleculares

2006 ◽  
Author(s):  
◽  
Marta Zulema Galván
Keyword(s):  
Phaseolus Vulgaris ◽  
Phaseolus Vulgaris L

El poroto común (Phaseolus vulgaris L.) es una leguminosa originaria de América y representa un componente importante en la dieta de la población latinoamericana por su alto contenido en proteínas y carbohidratos. El poroto cultivado se originó de los porotos silvestres, que son plantas anuales, herbáceas y trepadoras, que se distribuyen desde el norte de México hasta el noroeste de la Argentina. Estudios basados en caracteres morfológicos, bioquímicos y moleculares revelaron que tanto dentro de las variedades cultivadas como de las silvestres existen dos acervos génicos principales, uno Andino y el otro Mesoamericano. Las provincias del Noroeste Argentino (NOA) albergan un gran número de porotos silvestres y de variedades locales tradicionales (primitivas o “landraces”) mantenidas durante años en un sistema de cultivo tradicional. Sin embargo muchas de estas poblaciones están en peligro de extinción debido a las presiones de explotación forestal y pastoreo existentes en la zona, por lo que resulta de fundamental importancia su recolección y estudio. En esta tesis se analizó la variabilidad genética de un grupo de poblaciones silvestres y primitivas de poroto común del NOA, recolectadas en diferentes sitios de las provincias fitogeográficas de las Yungas, Prepuna y del Chaco, entre los 1300 y 2900 msnm, utilizando marcadores bioquímicos (proteínas de reserva de las semillas: faseolinas) y moleculares (RAPD e ISSR).

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