Effect of salt stress on antioxidant defence system in soybean root nodules

1998 ◽  
Vol 25 (6) ◽  
pp. 665 ◽  
Author(s):  
María E. Comba ◽  
María P. Benavides ◽  
María L. Tomaro
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Glutathione Reductase ◽  
Ascorbate Peroxidase ◽  
Reduced Glutathione ◽  
Nitrogenase Activity ◽  
Reductase Activity ◽  
Saline Stress ◽  
Antioxidant Defence ◽  
Antioxidant Defence System

The antioxidant defence systems of soybean (Glycine max (L.) Merr) nodules responded differently to 50 and 200 mM NaCl. At 50 mM NaCl, leghaemoglobin content and nitrogenase activity remained unaltered but there was an overall increase in the antioxidant enzymes (ascorbate peroxidase, catalase, glutathione reductase and superoxide dismutase) and in reduced glutathione. After returning the salinised nodules to a non-saline environment (recovery), the enzymatic activities returned to the initial values but reduced glutathione remained high with respect to the controls measured at the end of the experiment (final controls). Severe salt treatment reduced the leghaemoglobin content and nitrogenase activity by 31% and 50%, respectively. Ascorbate peroxidase, catalase and glutathione reductase activities decreased between 30 and 100% while superoxide dismutase and reduced glutathione increased over the controls by 19% and 30% respectively. After recovery, glutathione reductase increased over the final controls and reduced glutathione remained as under 50 mM NaCl. Malondialdehyde content and total protein remained unchanged in nodules treated with the two salt concentrations. These results suggest that under mild saline stress, the elevated levels of the antioxidant enzymes and reduced glutathione protect nodules against the activated oxygen species thus avoiding lipid and protein peroxidation, and leghaemoglobin breakdown. However, severe saline treatment produced an irreversible decay in the leghaemoglobin content and nitrogenase activity despite the high reduced glutathione level and glutathione reductase activity.

scholarly journals The relationship of antioxidant enzymes and some physiological parameters in maize during chilling

2011 ◽  
Vol 50 (No. 1) ◽  
pp. 27-32 ◽  
Author(s):  
T. Takáč
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Glutathione Reductase ◽  
Ascorbate Peroxidase ◽  
Reductase Activity ◽  
Physiological Parameters ◽  
Maize Seedlings ◽  
Maize Cultivars ◽  
Inhibition Of Growth ◽  
Glutathione Reductase Activity

The changes in some physiological parameters of maize seedlings in response to chilling were studied. The emphasis was laid upon their relationship to chilling induced alterations in antioxidant enzymes (superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase) activity. The exposure of maize seedlings to chilling caused substantial defects in the 4-day-old seedlings and the seedlings with two fully developed leaves, respectively. The membrane semipermeability perturbations and the loss of viability in the young seedlings were observed. Similarly, we found a decrease of chlorophyll content, appearance of necrotic lesions and inhibition of growth in older plants. The measurements of superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase activities provide an evidence of reactive oxygen species formation, that is assumed to be a reason of the found damages. Significant differences between two cultivars were found in the studied parameters. The electrolyte leakage and viability test provided effective methods for the characterization of the chilling tolerance-level in maize cultivars.

Relationship between antioxidant defence systems and salt tolerance in Solanum tuberosum

2000 ◽  
Vol 27 (3) ◽  
pp. 273 ◽  
Author(s):  
María P. Benavídes ◽  
Patricia L. Marconi ◽  
Susana M. Gallego ◽  
María E. Comba ◽  
María L. Tomaro
Keyword(s):  
Superoxide Dismutase ◽  
Solanum Tuberosum ◽  
Salt Tolerance ◽  
Reduced Glutathione ◽  
Solanum Tuberosum L ◽  
Dehydroascorbate Reductase ◽  
Antioxidant Defence ◽  
Defence System ◽  
Sensitive Clone ◽  
Antioxidant Defence System

A relationship between the antioxidant defence system and salt tolerance in two clones of potato (Solanum tuberosum L.) differing in salt sensitivity was studied. The antioxidant defence system of the sensitive clone responded differently to 100 and 150 mM NaCl. At 100 mM NaCl, growth, dehydroascorbate reductase and catalase activities remained unaltered, but chlorophyll and reduced glutathione content decreased (23% and 35%, respectively), while ascorbate content and superoxide dismutase activity were increased 34% and 63%, with respect to the control (0 mM NaCl). The superoxide dismutase increment was higher under 150 mM NaCl treatment, while a general decrease (except for dehydroascorbate reductase and catalase activities) in all the antioxidant parameters studied was observed in the sensitive clone. Reduced glutathione and ascorbate, the main antioxidant soluble defences, and all antioxidant enzymes (except catalase) were significantly elevated in the tolerant clone compared to the sensitive one when both were grown in the absence of NaCl. Under 100 and 150 mM NaCl treatments, no changes in the antioxidant stress parameters were detected in the tolerant clone. These results suggest a relationship between salt tolerance and the antioxidant defence system in the two clones.

scholarly journals Study of activity of enzymes of antioxidant system of blood at HIV infection

2010 ◽  
Vol 56 (5) ◽  
pp. 596-601 ◽  
Author(s):  
V.V. Kostyushov ◽  
I.I. Bokal ◽  
S.A. Petrov
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Glutathione Peroxidase ◽  
Hiv Infection ◽  
Blood Serum ◽  
Glutathione Reductase ◽  
Oxidative Modification ◽  
Antioxidant Defence ◽  
Activity Of Enzymes

HIV infection is accompanied by activation of lipid peroxidation, oxidative modification of lipoprotein complexes, and a decrease in activity of antioxidant enzymes (superoxide dismutase (SOD), catalase and glutathione peroxidase (GP)) in blood serum. A significant increase of glutathione reductase observed under these conditions is considered as a function of the antioxidant defence. These changes were already seen at early (symptomless) stages of this disease, however, at manifested forms they were more pronounced.

scholarly journals A Short-Term Resistance Training Circuit Improved Antioxidants in Sedentary Adults with Down Syndrome

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
M. Rosety-Rodriguez ◽  
M. Bernardi ◽  
S. Elosegui ◽  
I. Rosety ◽  
A. J. Diaz ◽  
...  
Keyword(s):  
Down Syndrome ◽  
Resistance Training ◽  
Oxidative Damage ◽  
Glutathione Reductase ◽  
Reductase Activity ◽  
Community Support ◽  
Knee Extensors ◽  
Antioxidant Defence ◽  
Defence System ◽  
Antioxidant Defence System

Previous studies have found aerobic training improved oxidative damage in people with Down syndrome (DS). However, there is a lack of information regarding the influence of resistance training on redox imbalance in this population. Accordingly, this study was conducted to determine the effect of resistance training (RT) on antioxidant defence system in sedentary adults with DS. Thirty-six male adults with DS were recruited through different community support groups. Eighteen were randomly assigned to perform a circuit RT program with 6 stations, 3 days/week for 12 weeks. Plasma total antioxidant status (TAS), reduced glutathione (GHS), ascorbate, serum α-tocopherol, and erythrocyte glutathione reductase activity were assessed. Plasma malondialdehyde (MDA) and carbonyl groups (CG) were assessed as markers of oxidative damage. Muscle strength was also measured. Dynamic torque of knee extensors and flexors as well as maximal handgrip strength was significantly improved after the completion of the training program. Plasma levels of TAS and erythrocyte glutathione reductase (GR) activity were significantly increased. Conversely, MDA and CG levels were significantly reduced. It was concluded RT improved antioxidant defence system and reduced oxidative damage in adults with DS. Further, long-term studies are required to determine whether the increased antioxidant system may improve clinical outcomes of adults with DS.

scholarly journals Effect of Cross-Sex Hormonal Replacement on Antioxidant Enzymes in Rat Retroperitoneal Fat Adipocytes

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Israel Pérez-Torres ◽  
Verónica Guarner-Lans ◽  
Alejandra Zúñiga-Muñoz ◽  
Rodrigo Velázquez Espejel ◽  
Alfredo Cabrera-Orefice ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Enzymatic Activities ◽  
Glutathione S Transferase ◽  
Control Groups ◽  
Intact Female ◽  
Hormonal Replacement

We report the effect of cross-sex hormonal replacement on antioxidant enzymes from rat retroperitoneal fat adipocytes. Eight rats of each gender were assigned to each of the following groups: control groups were intact female or male (F and M, resp.). Experimental groups were ovariectomized F (OvxF), castrated M (CasM), OvxF plus testosterone (OvxF + T), and CasM plus estradiol (CasM + E2) groups. After sacrifice, retroperitoneal fat was dissected and processed for histology. Adipocytes were isolated and the following enzymatic activities were determined: Cu-Zn superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and glutathione reductase (GR). Also, glutathione (GSH) and lipid peroxidation (LPO) were measured. In OvxF, retroperitoneal fat increased and adipocytes were enlarged, while in CasM rats a decrease in retroperitoneal fat and small adipocytes are observed. The cross-sex hormonal replacement in F rats was associated with larger adipocytes and a further decreased activity of Cu-Zn SOD, CAT, GPx, GST, GR, and GSH, in addition to an increase in LPO. CasM + E2exhibited the opposite effects showing further activation antioxidant enzymes and decreases in LPO. In conclusion, E2deficiency favors an increase in retroperitoneal fat and large adipocytes. Cross-sex hormonal replacement in F rats aggravates the condition by inhibiting antioxidant enzymes.

scholarly journals Antioxidant Effect of MnTE-2-PyP on Lung in Asthma Mice Model

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Lyudmil Terziev ◽  
Violeta Dancheva ◽  
Veneta Shopova ◽  
Galya Stavreva
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Control Group ◽  
Mice Model ◽  
Antioxidant Defence ◽  
Group 4 ◽  
Antioxidant Defence System ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Aim. To investigate the effects of MnTE-2-PyP on some markers of antioxidant defence system in asthma mice model.Material and Methods. The animals were divided into four groups: group 1, controls; group 2, injected with ovalbumin, group 3, treated with MnTE-2-PyP, and group 4, treated with ovalbumin and MnTE-2-PyP. The activities of superoxide dismutase, catalase, glutathione peroxidase and nonprotein sulfhydryl groups content (NPSH) were determined in lung homogenate.Results. The activities of superoxide dismutase and catalase in group 2 decreased significantly as compared to control group. The decrease of the same enzymes in group 4 was lower and significant as compared to group 2. Changes in the glutathione peroxidase activity showed a similar dynamics. The NPSH groups content decreased in group 2. In group 4 this decrease was relatively lower as compared to group 2.Conclusions. The application of MnTE-2-PyP mitigated the effects of oxidative stress in asthma mice model.

scholarly journals Health benefits of new symbiotic “NAR”

2014 ◽  
Vol 2 ◽  
Author(s):  
Saule Saduakhasova ◽  
Almagul Kushugulova ◽  
Samat Kozhakhmetov ◽  
Gulnara Shakhabayeva ◽  
Adil Supiyev ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Glutathione Reductase ◽  
Inflammatory Mediators ◽  
Antioxidant Activities ◽  
Reductase Activity ◽  
Protein A ◽  
Intact Cells ◽  
Total Antioxidant

Introduction: The immune-modulatory effects of synbiotics and their ability to reduce free radical levels may be useful for functional food that is able to be active throughout whole period of colonization of the gastrointestinal tract.The aim of the present study was to investigate the immune-modulatory and antioxidant effects of the synbiotic product "NАR," a probiotic beverage.Methods: The presence of IL-2, IL-4, IL-6, IL-8, IL-10, αTNF, γIFN, Ig A, Ig M, and Ig E was studied in vitro using a solid immunosorbent analysis. The total antioxidant activities of superoxide dismutase and glutathione reductase were determined by a spectrophotometry using the Sigma-Aldrich sets.Results: Studies of the immune-modulatory properties of the synbiotic product NAR showed 1.7 fold increase of γINF levels (p<0.01) in blood after consumption of the synbiotic product “NAR” in comparison to control values, whereas the concentrations of IL-4 and Ig E decreased 2.0 times (treatment: 9.3; control: 18.7; p<0.01) and 1.3 times (p<0.1), respectively. The consumption of the synbiotic product “NAR” caused an increase in the proportion of γINF/IL 4 (treatment: 15.4; control: 4.4; p<0.01), which indicates a reduction in functional activity of Th2-type lymphocytes in comparison with the function of Th1 cells.Our study showed a high level of the total antioxidant activity of the synbiotic product (67.4 mmol/ml). The antioxidant activity of the intact cells of consortium (15.3 mM/ml), which was the basis for the preparation of the symbiotic product, is several times lower than the activity observed in the symbiotic samples.Expression of SOD is one of the mechanisms of antioxidant stress radicals inactivation by bacteria. The analysis identified a superoxide dismutase activity of synbiotic product (1.42 U/mg protein). A glutathione reductase activity of the synbiotic product was elevated (0.06 U/ml). Conclusion: The majority of the inflammatory mediators found in the blood after the consumption of symbiotic product NAR were inflammatory mediators that activate a cellular component of the resistance. Moreover, the symbiotic product has a high antioxidant activity. 

Relationship between Activities of Enzymes for the Removal of O2·- and H2O2 and Nitrogenase in Root Nodules of Phaseolus vulgaris L.

1995 ◽  
Vol 50 (7-8) ◽  
pp. 543-551
Author(s):  
Bernhard Epping ◽  
Alexander P. Hansen ◽  
Peter Martin
Keyword(s):  
Superoxide Dismutase ◽  
Phaseolus Vulgaris ◽  
Ascorbate Peroxidase ◽  
Peroxidase Activity ◽  
Rhizobium Leguminosarum ◽  
Nitrogenase Activity ◽  
Root Nodules ◽  
Hypersensitive Reaction ◽  
Guaiacol Peroxidase ◽  
Bacterial Strains

Abstract Nodules of Rhizobium leguminosarum bv. phaseoli in symbiosis with Phaseolus vulgaris were compared with regard to their nitrogenase activity and activities of enzymes involved in the removal of O2·- and H2O2 as well as total ascorbate content. Activities of catalase (EC 1.11.1.6), ascorbate peroxidase (EC 1.11.1.11), and total ascorbate content were consist­ently higher in nodules inhabited by bacterial strains with higher nitrogenase activity. Values for superoxide dismutase (EC 1.15.11), and guaiacol peroxidase activity did not differ for the bacterial strains compared. On the other hand, when different plant cultivars were inoculated with the same bacterial strain, high nitrogenase activity did not correlate with a higher activ­ity of the oxygen scavenging enzyms or a higher content of total ascorbate. In this case, values for guaiacol peroxidase activity were greatly enhanced in nodules with lower nitrogen­ ase activity. This may be part of a hypersensitive reaction of the plant cultivar against the bacterial symbiotic partner. Inhibition of catalase activity in the nodules by addition of triazole to the nutrient solution did not alter nitrogenase activity within the first nine hours after addition. It can be concluded that the activity of catalase, ascorbate peroxidase, and superoxide dismutase is not generally coupled to nitrogenase activity in root nodules of P. vulgaris.

Effect of pineal indoles on activities of the antioxidant defense enzymes superoxide dismutase, catalase, and glutathione reductase, and levels of reduced and oxidized glutathione in rat tissues

2000 ◽  
Vol 78 (4) ◽  
pp. 447-453 ◽  
Author(s):  
F Liu ◽  
T B Ng
Keyword(s):  
Superoxide Dismutase ◽  
Body Weight ◽  
Glutathione Reductase ◽  
Intraperitoneal Injection ◽  
Reduced Glutathione ◽  
Rat Tissues ◽  
Sprague Dawley ◽  
Pineal Indoles ◽  
Antioxidant Defense Enzymes ◽  
Reduced And Oxidized Glutathione

Male Sprague-Dawley rats were randomly divided into four groups. Two of the groups received a single intraperitoneal injection of melatonin and 5-methoxytryptamine (5 mg/kg body weight), respectively, at 9 PM. One group received an intraperitoneal injection of 5-methoxytryptophol (5 mg/kg body weight) at 9 AM. The remaining group received alcoholic saline (vehicle) and served as the control. All rats were sacrificed 90 min after injection and the livers, kidneys, and brains were dissected. The activities of superoxide dismutase, catalase, and glutathione reductase in the organs were measured. It was found that both melatonin and 5-methoxytryptamine were approximately equipotent in enhancing the activities of superoxide dismutase and glutathione reductase in the kidney and liver, while 5-methoxytryptophol displayed a weaker effect. Both melatonin and 5-methoxytryptamine augmented the level of reduced glutathione in the kidney and liver, while 5-methoxytryptophol did so only in the kidney. All three pineal indoles increased the activity of superoxide dismutase and lowered the ratio of oxidized to reduced glutathione in the brain.Key words: pineal indoles, catalase, superoxide dismutase, glutathione reductase.

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