Spatial and temporal regulation of a soybean (Glycine max) lectin promoter in transgenic cotton (Gossypium hirsutum)

2002 ◽  
Vol 29 (7) ◽  
pp. 835 ◽  
Author(s):  
Belinda J. Townsend ◽  
Danny J. Llewellyn
Keyword(s):  
Glycine Max ◽  
Gossypium Hirsutum ◽  
Embryo Development ◽  
Promoter Activity ◽  
Gene Promoter ◽  
Transgenic Cotton ◽  
Gus Activity ◽  
Mature Seed ◽  
Lectin Gene ◽  
Lectin Promoter

The activity of a soybean (Glycine max L. Merrill) lectin gene promoter was investigated in transgenic cotton plants (Gossypium hirsutum L.) with the view to using this promoter for the seed-specific alteration of gossypol, a secondary metabolite in cotton that has adverse effects on the nutritional value of cottonseed products like oil and protein-rich meal. Agrobacterium-mediated transformation generated stable transformants containing a construct with the lectin promoter fused to the β-glucuronidase reporter gene (pLeGUS). Fluorometric GUS assays and northern hybridization detected strong promoter activity during embryo development. GUS activity in developing embryos was detected as early as 10 d post-anthesis (dpa), peaking late in embryo maturation. Enzyme activity persisted in imbibed mature seed, and negligible activity remained detectable in the roots and cotyledons of 7-d-old seedlings. No GUS activity was detected in leaves and squares of mature plants. GUS transcripts increased during embryo development to peak about 35 dpa, declining to a low level in imbibed mature seed. No transcripts were detected in roots, cotyledons, leaves or squares. Histochemical GUS activity staining indicated promoter activity in all cells of the cotyledons, including the flattened cells of the gossypol glands, the presumed site of synthesis of gossypol. This study concluded that the soybean lectin gene promoter is a useful tool for the seed-specific expression of transgenes in cotton.

scholarly journals Fatty Acid Synthase Confers Tamoxifen Resistance to ER+/HER2+ Breast Cancer

Cancers ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 1132
Author(s):  
Javier A. Menendez ◽  
Adriana Papadimitropoulou ◽  
Travis Vander Steen ◽  
Elisabet Cuyàs ◽  
Bharvi P. Oza-Gajera ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Fatty Acid ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Promoter Activity ◽  
Fatty Acid Synthase ◽  
Endocrine Resistance ◽  
Gene Promoter ◽  
Her2 Positive ◽  
Her2 Breast Cancer

The identification of clinically important molecular mechanisms driving endocrine resistance is a priority in estrogen receptor-positive (ER+) breast cancer. Although both genomic and non-genomic cross-talk between the ER and growth factor receptors such as human epidermal growth factor receptor 2 (HER2) has frequently been associated with both experimental and clinical endocrine therapy resistance, combined targeting of ER and HER2 has failed to improve overall survival in endocrine non-responsive disease. Herein, we questioned the role of fatty acid synthase (FASN), a lipogenic enzyme linked to HER2-driven breast cancer aggressiveness, in the development and maintenance of hormone-independent growth and resistance to anti-estrogens in ER/HER2-positive (ER+/HER2+) breast cancer. The stimulatory effects of estradiol on FASN gene promoter activity and protein expression were blunted by anti-estrogens in endocrine-responsive breast cancer cells. Conversely, an AKT/MAPK-related constitutive hyperactivation of FASN gene promoter activity was unaltered in response to estradiol in non-endocrine responsive ER+/HER2+ breast cancer cells, and could be further enhanced by tamoxifen. Pharmacological blockade with structurally and mechanistically unrelated FASN inhibitors fully impeded the strong stimulatory activity of tamoxifen on the soft-agar colony forming capacity—an in vitro metric of tumorigenicity—of ER+/HER2+ breast cancer cells. In vivo treatment with a FASN inhibitor completely prevented the agonistic tumor-promoting activity of tamoxifen and fully restored its estrogen antagonist properties against ER/HER2-positive xenograft tumors in mice. Functional cancer proteomic data from The Cancer Proteome Atlas (TCPA) revealed that the ER+/HER2+ subtype was the highest FASN protein expressor compared to basal-like, HER2-enriched, and ER+/HER2-negative breast cancer groups. FASN is a biological determinant of HER2-driven endocrine resistance in ER+ breast cancer. Next-generation, clinical-grade FASN inhibitors may be therapeutically relevant to countering resistance to tamoxifen in FASN-overexpressing ER+/HER2+ breast carcinomas.

scholarly journals Haplotype Effects on Matrix Metalloproteinase-1 Gene Promoter Activity in Cancer Cells

2007 ◽  
Vol 5 (3) ◽  
pp. 221-227 ◽  
Author(s):  
Eve G. Pearce ◽  
Ross C. Laxton ◽  
Andresa C. Pereira ◽  
Shu Ye
Keyword(s):  
Matrix Metalloproteinase ◽  
Cancer Cells ◽  
Promoter Activity ◽  
Gene Promoter ◽  
Matrix Metalloproteinase 1

scholarly journals Repression of MHC class I gene promoter activity by two-exon Tat of HIV

Science ◽  
1993 ◽  
Vol 260 (5112) ◽  
pp. 1320-1322 ◽  
Author(s):  
T. Howcroft ◽  
K Strebel ◽  
M. Martin ◽  
D. Singer
Keyword(s):  
Mhc Class I ◽  
Promoter Activity ◽  
Gene Promoter ◽  
Class I ◽  
I Gene ◽  
Mhc Class I Gene

The nuclear orphan receptors COUP-TFII and Ear-2 act as silencers of the human oxytocin gene promoter

1997 ◽  
Vol 19 (2) ◽  
pp. 163-172 ◽  
Author(s):  
K Chu ◽  
HH Zingg
Keyword(s):  
Receptor Binding ◽  
Promoter Activity ◽  
Transcriptional Activity ◽  
Gene Promoter ◽  
Fine Tuning ◽  
Orphan Receptor ◽  
Expression Vectors ◽  
Mobility Shift ◽  
Interaction Sites

We have previously shown that COUP-TFII and Ear-2, two members of the nuclear orphan receptor family, are able to repress oestrogen-stimulated transcriptional activity of the human oxytocin (OT) gene promoter by binding to a site that overlaps with the oestrogen response element (ERE) present in the 5' flanking region of the gene. Although most nuclear receptor-mediated transcriptional repression conforms with the paradigm of passive repression and involves competitive binding to an activator site, active repression, i.e. silencing of basal promoter activity, has been observed in a limited number of cases. Here we show by co-transfection experiments using COUP-TFII and Ear-2 expression vectors and reporter constructs containing OT gene promoter fragments linked to the chloramphenicol acetyltransferase gene that both COUP-TFII and Ear-2 are capable of silencing basal OT gene promoter activity by 54 and 75% respectively. 5' Deletion and footprint analyses revealed two areas of functionally important interaction sites: (1) a direct TGACC(T/C) repeat overlapping the ERE and (2) a more promoter-proximal area centred at - 90 containing three imperfect direct repeats (R1-R3) spaced by four nucleotides each. Mutagenesis of reporter constructs as well as electrophoretic mobility-shift assays demonstrated that each of the three proximal repeats R1-R3 contributed to orphan receptor binding and the silencing effect. Inasmuch as the orphan receptor-binding sites are not involved in mediating basal transcriptional activity of the OT gene promoter, the observed effects are best interpreted as active repression or promoter silencing. Moreover, since COUP-TFII and Ear-2 are both co-expressed in OT-expressing uterine epithelial cells, the novel transcriptional effects described here are likely to be of functional importance in the fine-tuning of uterine OT gene expression in vivo.

Herbicidal Concentrations of Picloram in Cell Culture and Leaf Buds

Weed Science ◽  
1972 ◽  
Vol 20 (2) ◽  
pp. 185-188 ◽  
Author(s):  
F. S. Davis ◽  
A. Villarreal ◽  
J. R. Baur ◽  
I. S. Goldstein
Keyword(s):  
Cell Culture ◽  
Glycine Max ◽  
Gossypium Hirsutum ◽  
Cell Cultures ◽  
Tolerance Limit ◽  
Primary Leaf ◽  
Fresh Weight ◽  
Internal Concentration ◽  
Order Of Magnitude

Cell cultures of soybean(Glycine max(L.) Merrill ‘Acme’) were exposed to media containing 4-amino-3,5,6-trichloropicolinic acid (picloram) for 15 days. Picloram also was supplied once in droplets (water) to cotyledons of 10 to 13-day-old seedlings of cotton(Gossypium hirsutumL. ‘Champion’). The amounts of picloram necessary to reach and exceed the 50% tolerance limit (TL50) of the cell cultures (inhibition) and of the primary leaf buds (toxicity) were established, and internal picloram concentrations then were determined. Internal concentrations at the TL50were 0.17 nM/g fresh weight and 14.7 nM/g fresh weight for cell cultures and leaf buds, respectively. These values are approximately 10−7and 10−5molar. In leaf buds, concentrations increased rapidly for 36 hr after treatment and declined slowly thereafter. Primary leaf buds accumulated up to several times the lethal internal concentration of picloram when the dosage to the cotyledons was increased by one order of magnitude.

scholarly journals KERAPATAN GALUR HARAPAN KAPAS PADA SISTEM TUMPANGSARI DENGAN KEDELAI

2020 ◽  
Vol 9 (1) ◽  
pp. 11
Author(s):  
PRIMA DIARINI RIAJAYA ◽  
FITRININGDYAH TRI KADARWATI
Keyword(s):  
Glycine Max ◽  
Gossypium Hirsutum ◽  
Field Experiment ◽  
Key Words ◽  
Photosynthetic Efficiency ◽  
Cropping Systems ◽  
Cotton Yield ◽  
Systems Research ◽  
Crop Density ◽  
Plot Design

<p>Penelitian pengaturan kerapalan galur harapan kapas pada sistem tumpangsari dengan kedelai dilakukan di IPPTP Mojosari, Mojokerto, Jawa Timur pada lahan sawah sesudah padi dari bulan Mei sampai dengan Oktobcr 2000. Tujuan penelitian untuk mendapatkan kerapalan lanaman yang sesuai pada galur harapan kapas pada sistem tumpangsari dengan kedelai Percobaan disusun dalam rancangan petak tcrbagi dengan 4 varictas'galur (92016/6, 91001 29 2, 88003/16/2 dan Kanesia 7) sebagai pelak utama Anak petak terdiri atas 3 tata tanam yaitu (1) tala tanam 1(1); 3, yaitu I bans kapas (I tan 'lubang) dan 3 bais kedelai, (2) tata tanam 2 (1) 4 yaitu 2 baris kapas(l tan.'lubang) dan 4 baris kedelai, (3) tata tanam 1 (2)3 yaitu 1 baris (2 tan 'lubang dan 3 bais kedelai) Jarak lanam kapas dan kedelai pada (ala tanam 1(1) 3 adalah 150 x 20 cm dan 25 x 20 cm, pada tata tanam 2( I ):4 adalah 150 (60) cm x 30 cm dan 20 cm x 20 cm, dan tata tanam 1 (2) 3 adalah 150 cm x 30 cm dan 25 cm x 20 cm Hasil penelitian menunjukkan bahwa lata tanam yang sesuai pada galur varietas baru kapas adalah tata tanam 1(1)3 |1 baris kapas (1 tan lubang) dan tiga baris kedelai] Mengurangi jumlah lanaman kapas tiap lubang dari 2 menjadi I lanaman pada tata tanam 1 (2)3 (1 baris kapas (2 lan lubang) dan 3 bais kedelai) meningkatkan eisiensi fotosintcsis dai 59 x 10 menjadi 9.4 x 10"" mgC02.mgll20 sehingga produksi kapas meningkat dari 1 167 2 menjadi I 251 6 kgha, sedangkan produksi kedelai tidak berpengaruh yaitu rata-rata 846 kgha Apabila dialur dalam sistem 2:4 (2 baris kapas diantara 4 baris kedelai), maka eisiensi fotosintcsis hanya meningkat dari 5.9 x \0A menjadi 77 x 10 mg C02mg H20 sehingga produksi kapas hanya meningkat dari I 167 2 menjadi I 206 2 kgha Pada kedua sistem lanam tersebut produktivitas galur 8800316/2 (1 323.3 kgha) lidak berbeda dengan Kanesia 7 (I 365.2 kg/ha) dan nyata lebih tinggi daripada galur 920166 (1 096 9 kgha) maupun 91001.29/2 (1 048 0 kgha).</p><p>Kata kunci: Gossypium hirsutum. kapas. Glycine Max, kedelai, kerapatan lanaman, tumpangsari, hasil</p><p> </p><p><strong>ABSTRACT</strong></p><p><strong>Density of neyv cotton lines under intercropping system with soybean</strong></p><p>The ield trial on different crop densities for new cotton lines under intercropping system with soybean was conducted in Mojosari. East Java from May lo October 2000 on the rice ield ater harvest. The purpose of the study was to investigate die optimum population for new cotton lines under intercropping with soybean The field experiment was arranged in a Split Plot Design with three replications. Pour new cotton lines were allocated lo main plots 92016 6, 91001/29.2 (okra leal). 88003/16/2 and Kanesia 7 'Three crop arrangements were allocated to sub-plots: 1 (1 ):3 [1 cotton row (I plant/hole) in between 3 rows of soybean), 2(1 ):4 [ 2 coton rows (1 plant/hole) in between 4 rows of soybean, and 1(2):3 (1 cotton row (2 planlholc) in between 3 rows of soybean). Two replications for sole crops of cotton and soybean were included in this expeiment lo compare both cropping systems. Research showed that by keeping one cotton plant/hole under intercropping system wi(h soybean in arrangement of 1:3 11 conon row in between 3 rows of soybean), increased the photosynthetic efficiency from 5 9 x 10"* to 9.4 x 10"* mg C02/mg H20, causing cotton yield increased from 1167.2 to 1 251.6 kg/ha; however soybean yield did not differ between different propotions of cotton and soybean (846 kg/ha) Under arrangement of Iwo cotton rows * four rows of soybean, the photosynthetic efficiency increased from 5.9 x 10"1 to 7.7 x 10"* mg COj'mg HjO resulted in increased cotton yield from I 167.2 lo 1 206.2 kgha Ihe yield of line 88003/16 2 (1 323.3 kgha) did not differ with that on Kanesia 7 (I 365.2 kg/ha); both were higher than those on 92016/6 (1 096.9 kg/ha) and 91001 /29/2 (1 048.0 kgha).</p><p>Key words: Gossypium hirsutum, kapas. Glycine Max, soybean, crop density, intercropping, yield</p>

scholarly journals Upstream region of rat serum albumin gene promoter contributes to promoter activity: presence of functional binding site for hepatocyte nuclear factor-3

1999 ◽  
Vol 338 (2) ◽  
pp. 241-249 ◽  
Author(s):  
Chin-Hui HSIANG ◽  
Norman W. MARTEN ◽  
Daniel S. STRAUS
Keyword(s):  
Serum Albumin ◽  
Hepg2 Cells ◽  
Promoter Activity ◽  
Gene Promoter ◽  
Upstream Region ◽  
Nuclear Factor ◽  
Hepatocyte Nuclear Factor ◽  
Rat Serum ◽  
Albumin Gene ◽  
Rat Serum Albumin

Transcription of the serum albumin gene occurs almost exclusively in the liver and is controlled in part by a strong liver-specific promoter. The upstream region of the serum albumin gene promoter is highly conserved among species and is footprinted in vitro by a number of nuclear proteins. However, the role of the upstream promoter region in regulating transcription and the identity of the transcription factors that bind to this region have not been established. In the present study, deletion analysis of the rat serum albumin promoter in transiently transfected HepG2 cells demonstrated that elimination of the region between -207 and -153 bp caused a two-fold decrease in promoter activity (P< 0.05). Additional analysis of the -207 to -124 bp promoter interval led to the identification of two potential binding sites for hepatocyte nuclear factor-3 (HNF-3) located at -168 to -157 bp (site X) and -145 to -134 bp (site Y). Electrophoretic mobility-shift assays performed with the HNF-3 X and Y sites demonstrated that both sites are capable of binding HNF-3α and HNF-3β. Placement of a single copy of the HNF-3 X site upstream from a minimal promoter increased promoter activity by about four-fold in HepG2 cells, and the reporter construct containing this site could be transactivated if co-transfected with an HNF-3 expression construct. Furthermore, inactivation of the HNF-3 X site by site-directed mutagenesis within the context of the -261 bp albumin promoter construct resulted in a 40% decrease in transcription (P< 0.05). These results indicate that the positive effect of the -207 to -153 bp promoter interval is attributable to the presence of the HNF-3 X site within this interval. Additional results obtained with transfected HepG2 cells suggest that the HNF-3 Y site plays a lesser role in activation of transcription than the X site.

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