Naming species with no morphological indicators: species status of Galeolaria caespitosa (Annelida:Serpulidae) inferred from nuclear and mitochondrial gene sequences and morphology

2009 ◽  
Vol 23 (3) ◽  
pp. 205 ◽  
Author(s):  
Magdalena N. Halt ◽  
Elena K. Kupriyanova ◽  
Steven J. B. Cooper ◽  
Greg W. Rouse
Keyword(s):  
Cytochrome B ◽  
Mitochondrial Gene ◽  
Taxonomic Status ◽  
Molecular Data ◽  
Pairwise Distance ◽  
South Wales ◽  
Western Group ◽  
Mitochondrial Cytochrome B ◽  
Group Variation ◽  
Morphological Differences

Galeolaria caespitosa Lamarck, 1818 is an endemic, gregarious serpulid annelid, ubiquitous along the intertidal zone of southern Australia, occurring from Queensland to Western Australia. We sampled specimens across this range and utilised morphological features and sequences of mitochondrial (cytochrome-b) and nuclear (ITS2) markers to assess the taxonomic status of this morphospecies. No taxonomically significant morphological differences were observed across the range of G. caespitosa. However, the molecular data revealed the existence of the following two well supported clades that were also geographically concordant for the two markers: an eastern clade, consisting of New South Wales and southern Queensland samples, and a south-western group that encompassed samples from the rest of its range. The minimum pairwise distance between members of the two groups was more than 24% for cytochrome-b, with a maximum of 1% within-group variation. In addition, analysis of molecular variation showed a high proportion (97%) of the total variation distributed among the two groups, indicative of long-term isolation of the two clades. These results suggest that G. caespitosa comprises at least two cryptic species. Here, we discuss the merits of naming new Galeolaria species, given there were no consistent morphological differences detectable and the absence of details on the type locality for G. caespitosa. We conclude that a new species of Galeolaria is warranted and describe it here as G. gemineoa.

Notes on the molecular taxonomy of the Proclossiana eunomia complex (Lepidoptera, Nymphalidae: Argynnini): analysis of DNA barcodes

2018 ◽  
Vol 8 (1) ◽  
pp. 222-232 ◽  
Author(s):  
R. V. Yakovlev ◽  
N. A. Shapoval ◽  
G. N. Kuftina ◽  
A. V. Kulak ◽  
S. V. Kovalev
Keyword(s):  
Body Size ◽  
Molecular Taxonomy ◽  
Taxonomic Status ◽  
Molecular Data ◽  
Coi Gene ◽  
Wing Pattern ◽  
Molecular Approach ◽  
Dna Barcodes ◽  
Morphological Differences ◽  
Proclossiana Eunomia

The Proclossiana eunomia (Esper, 1799) complex is currently composed of the several subspecies distributed throughout Palaearсtic region and North America. Despite the fact that some of the taxa have differences in wing pattern and body size, previous assumptions on taxonomy not supported by molecular data. Therefore, the identity of certain populations of this complex has remained unclear and the taxonomic status of several recently described taxa is debated. Here, we provide insights into systematics of some Palaearctic members of this group using molecular approach, based on the analysis of the barcoding fragment of the COI gene taking into account known morphological differences.

The taxonomic status of the Castelo dos Sonhos Hantavirus reservoir, Oligoryzomys utiaritensis Allen 1916 (Rodentia: Cricetidae: Sigmodontinae)

Zootaxa ◽  
2012 ◽  
Vol 3220 (1) ◽  
pp. 1 ◽  
Author(s):  
RODRIGO AGRELLOS ◽  
CIBELE R. BONVICINO ◽  
ELIZABETH SALBÉ T. ROSA ◽  
APARECIDO A.R. MARQUES ◽  
PAULO S. D’ANDREA ◽  
...  
Keyword(s):  
Cytochrome B ◽  
Diploid Number ◽  
Phylogenetic Analyses ◽  
Taxonomic Status ◽  
Sympatric Species ◽  
Junior Synonym ◽  
Natural Reservoir ◽  
Nuclear Intron ◽  
Phylogenetic Reconstructions ◽  
Mitochondrial Cytochrome B

Species of the genus Oligoryzomys are commonly found accross Latin America, and several of them play important rolesas natural reservoirs of Hantaviruses. Here we demonstrate that O. utiaritensis, the natural reservoir of hantavirus Castelodos Sonhos in northwestwern Brazil and previously considered a junior synonym of O. nigripes or O. eliurus, is a validspecies. Morphology, morphometry, karyotyping, and phylogenetic reconstructions based on nuclear (intron 7 of the beta-fibrinogen gene) and mitochondrial (cytochrome b) DNA show that O. utiaritensis differs from O. nigripes and from otherforms of the genus, including the recently described taxon O. moojeni. Oligoryzomys utiaritensis differs in external (whit-ish ventral pelage and tail weakly bicolored) and cranial (incisive foramina never extending posteriorly the alveolus lineof M1) characters from sympatric species. It has the highest diploid number (2n=72) within Oligoryzomys, and is charac-terized by three putative synapomorphies in cytochrome b, and one in intron 7 of beta fibrinogen. We also point to theassignment of Oligoryzomys eliurus as a junior synonym of O. nigripes. Finally, we present phylogenetic analyses of in-trageneric relationships showing that O. utiaritensis is a member of a clade containing Amazonian and Cerrado taxa, including O. moojeni, O. rupestris, and O. delicatus.

scholarly journals A new species of Gonyosoma Wagler, 1828 (Serpentes, Colubridae), previously confused with G. prasinum (Blyth, 1854)

2021 ◽  
Vol 5 (1) ◽  
pp. 129-139
Author(s):  
Shuo Liu ◽  
Mian Hou ◽  
Ye Htet Lwin ◽  
Qiaoyan Wang ◽  
Dingqi Rao
Keyword(s):  
New Species ◽  
Bayesian Inference ◽  
Cytochrome B ◽  
Yunnan Province ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Pairwise Distance ◽  
Mitochondrial Cytochrome ◽  
Mitochondrial Cytochrome B ◽  
A New Species

A new species of the genus Gonyosoma Wagler is described from Yunnan Province, China. The new species closely resembles G. prasinum (Blyth), but it is differentiated from the latter species by the following characters: precloacal plate divided, iris blue and inside of mouth greyish-white in life. Based on phylogenetic analyses of mitochondrial cytochrome b sequence data, the new species is recovered as the sister species to G. prasinum by Bayesian Inference and Maximum Likelihood analyses. The uncorrected pairwise distance between the new species and other species of the genus Gonyosoma ranged from 11.78% to 17.07% calculated using the mitochondrial cytochrome b sequence. This discovery increases the number of Gonyosoma species to seven.

scholarly journals Phylogeography of a Darevskia (caucasica) complex (Lacertidae: Sauria) based on the cytochrome b mitochondrial gene analysis

2021 ◽  
Vol 325 (1) ◽  
pp. 49-66
Author(s):  
I.V. Doronin ◽  
P.A. Dzhelali ◽  
K.Yu. Lotiev ◽  
L.F. Mazanaeva ◽  
G.A. Mustafaeva ◽  
...  
Keyword(s):  
Cytochrome B ◽  
Mitochondrial Gene ◽  
Mitochondrial Cytochrome ◽  
Mitochondrial Marker ◽  
South Ossetia ◽  
Independent Form ◽  
Mitochondrial Cytochrome B ◽  
Short Period ◽  
Single Clade ◽  
Mitochondrial Cytochrome B Gene

The range of superspecific complex of rock lizards Darevskia (caucasica) (D. alpina, D. c. caucasica, D. c. vedenica, D. daghestanica) includes the Main Caucasian ridge and its spurs on the southern and northern macroslopes within heights from 500 m to 3200 m above sea level. A fragment of the mitochondrial cytochrome b gene (1096 bp) was analyzed in 48 specimens of these lizards. Specimens were collected in 23 localities. We have found 39 unique haplotypes. The minimum genetic diversity was found in D. c. vedenica, maximum in D. c. caucasica. All taxa of the complex, except for D. c. vedenica, demonstrate genetic heterogeneity. Four clades can be distinguished on the dendrogram of phylogenetic relationships. They correspond to D. alpina, D. c. caucasica, D. c. vedenica, D. daghestanica. D. c. caucasica forms two distinct haplogroups. On the phylogenetic tree, the sample of D. c. vedenica formed an independent clade close to D. c. caucasica. The Darevskia (caucasica) complex is polyphyletic according to the analysis of the mitochondrial marker. Nucleotide sequences of D. alpina formed a single clade with Darevskia (saxicola) complex representatives and combined with the sequences of D. brauneri into subclade. On the example of the population of D. daghestanica, found in a forest biotope without rocky outcrops and genetically not differing from individuals from the adjacent territory, the ability of rocky lizards to master biotopes unusual for them in a short period of time was demonstrated. This example demonstrates the ability of rock lizards to adapt to unusual biotopes in a short period of time. Genetic distance between D. c. vedenica and D. c. caucasica is comparable to the distance between D. c. caucasica and D. daghestanica. We assume the existence of an independent form of D. caucasica ssp. inhabiting South Ossetia and adjacent territories.

scholarly journals Molecular data indicate a cryptic species in Neotoma albigula (Cricetidae: Neotominae) from northwestern México

2015 ◽  
Vol 97 (1) ◽  
pp. 187-199
Author(s):  
Robert D. Bradley ◽  
Matthew R. Mauldin
Keyword(s):  
Dna Sequences ◽  
Phylogenetic Analyses ◽  
Nuclear Gene ◽  
Molecular Data ◽  
Genetic Distances ◽  
Dehydrogenase Gene ◽  
Mitochondrial Cytochrome B ◽  
Northwestern Mexico ◽  
Morphological Differences ◽  
Intron 2

Abstract DNA sequences from the mitochondrial cytochrome- b gene were obtained from 41 specimens of Neotoma albigula from the southwestern United States and northwestern México. Phylogenetic analyses depicted that samples of N. a. melanura from southern Sonora and northern Sinaloa formed a clade separate from representatives of the other sampled subspecies of N. albigula ( albigula , laplataensis , mearnsi , seri , sheldoni , and venusta ). Genetic distances detected between these clades (7.41%) approached divergence levels reported for other sister species of woodrats and indicated that N. a. melanura is presumably a cryptic and genetically differentiated species relative to N . albigula . Analyses of DNA sequences from a nuclear gene (intron 2 of the alcohol dehydrogenase gene, Adh 1-I2) indicated that samples of N. a. melanura formed a separate, monophyletic clade relative to the remainder of N. albigula . Further, habitat, geographic distinctions, and morphological differences were apparent between members of the 2 clades. Together, those data support the elevation of N. a. melanura to species status. Secuencias de ADN del gen mitocondrial citocromo- b se obtuvieron de 41 especímenes de Neotoma albigula provenientes del suroeste de los Estados Unidos y del noroeste de México. Análisis filogenéticos revelaron que las muestras de N. a. melanura del sureste de Sonora y del norte de Sinaloa formaron un clado separado de especímenes que representan a las otras subspecies de N. albigula (albigula, laplataensis, mearnsi, seri, sheldoni , y venusta ). Las distancias genéticas detectadas entre estos clados (7.41%) se aproximan a los valores de divergencia reportados para otras especies de ratas magueyeras, indicando que N. a. melanura es presumiblemente una especie críptica y relativamente diferenciada genéticamente de N. albigula . Los análisis de secuencias de ADN de un gen nuclear (intron 2 del gen alcohol deshidrogenasa, Adh -I2) indicaron que las muestras de N. a. melanura formaron un clado separado relativamente a especímenes de N. albigula . Adicionalmente, hábitat, distinciones geográficas, y diferencias morfológicas fueron aparentes entre miembros de los dos clados. Todos estos datos soportan la elevación de N. a. melanura al estatus de especie.

Albumin and mitochondrial DNA evolution: Phylogenetic implications for colubrine snakes (Colubridae: Colubrinae)

1996 ◽  
Vol 17 (3) ◽  
pp. 247-259 ◽  
Author(s):  
T.J. Lopez ◽  
L.R. Maxson
Keyword(s):  
16S Rrna ◽  
Cytochrome B ◽  
Sequence Data ◽  
Phylogenetic Signal ◽  
Mitochondrial Gene ◽  
Mitochondrial Cytochrome B ◽  
Nucleotide Sequence Variation ◽  
Phylogenetic Implications ◽  
Rrna Sequences ◽  
16S Rrna Sequences

AbstractPrevious molecular analyses of colubrine snake relationships have been based on estimates of amino acid sequence differences in the nuclear-encoded protein, serum albumin. Phylogenetic hypotheses based on albumin data are compared to new trees derived from nucleotide sequence variation in a 307-base pair (bp) region of the mitochondrial cytochrome b gene and a 384-bp region of the mitochondrial gene encoding the 16S ribosomal RNA (rRNA) subunit. There are so many multiple substitutions at degenerate sites in the cytochrome b sequences that little phylogenetic signal remains, leaving major conclusions based on the more informative 16S rRNA sequences. Analyses of the sequence data using both parsimony and genetic distance algorithms are largely congruent with earlier conclusions based on albumin, and corroborate (1) the monophyly of the ratsnakes, (2) the derivation of the ratsnake clade from within the racer lineage, and (3) the monophyly of the Nearctic racer clade (Coluber + Masticophis). The 16S rRNA data provide greater resolution of relationships within the racer lineage than either the cytochrome b or albumin data. The trees based on cytochrome b and 16S rRNA sequences do not support monophyly of the racers, in contrast to the albumin data.

Phylogenetic and biogeographic relationships of gerbil mice Eligmodontia (Rodentia, Cricetidae) in South America, with a description of a new species

Zootaxa ◽  
2008 ◽  
Vol 1753 (1) ◽  
pp. 1 ◽  
Author(s):  
MICHAEL A. MARES ◽  
JANET K. BRAUN ◽  
BRANDI S. COYNER ◽  
RONALD A. VAN DEN BUSSCHE
Keyword(s):  
New Species ◽  
Cytochrome B ◽  
Sequence Data ◽  
Molecular Data ◽  
Cytochrome B Gene ◽  
Minimum Evolution ◽  
Nucleotide Sequence Data ◽  
Biogeographic History ◽  
Mitochondrial Cytochrome B ◽  
A New Species

We present a systematic study of the genus Eligmodontia (sigmodontine rodents of the tribe Phyllotini) from Argentina, Bolivia, and Chile, based on molecular data. Phylogenetic relationships among 49 individuals were examined using nucleotide sequence data from the entire 1143 bp of the mitochondrial cytochrome-b gene. Unweighted parsimony, minimum evolution, maximum-likelihood (TrN+I+G), and Bayesian analyses revealed 2 major clades, representing Andean and non-Andean groups, and 6 minor clades, representing E. typus (lowland), E. typus (highland), E. morgani, E. puerulus, E. moreni, and E. hirtipes. E. typus (highland) is described as a new species based on combined data from the cytochrome-b gene, morphology, and karyology. The results indicate that the genus Eligmodontia is composed of a complex of species, most of which correspond to taxa that were described originally and each of which shows molecular cohesion within a limited geographic range. This species complex has relevance to current theories regarding the speciation patterns and the historical biogeography of South American sigmodontine rodents. The biogeographic history of the genus also is outlined.

scholarly journals Notes on Vampyressa thyone (Chiroptera: Phyllostomidae): distribution, genetics and hypopigmentation

2019 ◽  
Vol 19 (2) ◽  
Author(s):  
Giovani Hernández-Canchola ◽  
Yire A. Gómez-Jiménez ◽  
Iván Hernández-Chávez ◽  
Sara Carolina Lucero-Verdugo ◽  
Livia León-Paniagua
Keyword(s):  
Cytochrome B ◽  
Species Distribution ◽  
Tropical Rainforest ◽  
Taxonomic Status ◽  
Taxonomic Revision ◽  
The State ◽  
Mitochondrial Cytochrome ◽  
New Mexican ◽  
Neotropical Species ◽  
Mitochondrial Cytochrome B

Abstract: The frugivorous bats of the genus Vampyressa include five neotropical species, and some of them are considered relatively rare and uncommonly encountered. The northernmost distribution of this genus is in Mexico where only one species, the monotypic Vampyressa thyone, is found. Here, we report the Northwesternmost record for this species, extending its distribution range to the state of Guerrero, Mexico. This record extends the limit of the species distribution by more than 153 km. We also compared this specimens with other V. thyone, and using mitochondrial cytochrome-b sequences we detected three geographic clades within the species. The new Mexican specimen represent the most divergent cytochrome-b sequence within V. thyone. We recommend a taxonomic revision to validate the taxonomic status of the three groups detected within V. thyone, particularly the differentiated Mexican clade. Additionally, we report a case of hypopigmentation in another V. thyone collected in a mature tropical rainforest in Chiapas, Mexico.

Adult and larval associations of the alpine stonefly genus Riekoperla McLellan (Plecoptera:Gripopterygidae) using mitochondrial DNA

2011 ◽  
Vol 25 (1) ◽  
pp. 11 ◽  
Author(s):  
J. H. Mynott ◽  
J. M. Webb ◽  
P. J. Suter
Keyword(s):  
Mitochondrial Dna ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Aquatic Insects ◽  
Sequence Data ◽  
New South ◽  
Mitochondrial Gene ◽  
Life Stage ◽  
Molecular Data ◽  
South Wales

The current taxonomic understanding of the genus Riekoperla McLellan, 1971 (Gripopterygidae) is poor, with 15 of the 28 species and subspecies having unknown or uncertain larval associations. Sequences of a 657 bp fragment from the mitochondrial gene cytochrome c oxidase subunit 1 (CO1) were obtained from 122 specimens of 13 species collected throughout the alpine areas of New South Wales and Victoria, Australia. Of these, sequence data associated adults and larvae for the following 10 species: R. alpina McLellan, 1971, R. cf. intermedia, R. compressa Theischinger, 1985, R. hynesorum Theischinger, 1985, R. karki McLellan, 1971, R. montana Theischinger, 1985, R. reticulata (Kimmins, 1951), R. rugosa (Kimmins, 1951), R. trapeza Theischinger, 1985, and R. tuberculata McLellan, 1971. Adults of R. intermedia Theischinger, 1985, R. triloba triloba McLellan, 1971 and R. williamsi McLellan, 1971 were sequenced but no larvae were associated with them. The 13 species were reciprocally monophyletic and had minimum interspecific sequence divergences ranging from 7.2–19.5%, higher than the maximum intraspecific sequence divergences (0.6–5.8%). The combination of morphology and molecular data enabled rapid life stage association for alpine Riekoperla species and this method should be used more frequently for other environmentally significant aquatic insects.

Sign in / Sign up

Export Citation Format

Share Document