Adult and larval associations of the alpine stonefly genus Riekoperla McLellan (Plecoptera:Gripopterygidae) using mitochondrial DNA

2011 ◽  
Vol 25 (1) ◽  
pp. 11 ◽  
Author(s):  
J. H. Mynott ◽  
J. M. Webb ◽  
P. J. Suter
Keyword(s):  
Mitochondrial Dna ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Aquatic Insects ◽  
Sequence Data ◽  
New South ◽  
Mitochondrial Gene ◽  
Life Stage ◽  
Molecular Data ◽  
South Wales

The current taxonomic understanding of the genus Riekoperla McLellan, 1971 (Gripopterygidae) is poor, with 15 of the 28 species and subspecies having unknown or uncertain larval associations. Sequences of a 657 bp fragment from the mitochondrial gene cytochrome c oxidase subunit 1 (CO1) were obtained from 122 specimens of 13 species collected throughout the alpine areas of New South Wales and Victoria, Australia. Of these, sequence data associated adults and larvae for the following 10 species: R. alpina McLellan, 1971, R. cf. intermedia, R. compressa Theischinger, 1985, R. hynesorum Theischinger, 1985, R. karki McLellan, 1971, R. montana Theischinger, 1985, R. reticulata (Kimmins, 1951), R. rugosa (Kimmins, 1951), R. trapeza Theischinger, 1985, and R. tuberculata McLellan, 1971. Adults of R. intermedia Theischinger, 1985, R. triloba triloba McLellan, 1971 and R. williamsi McLellan, 1971 were sequenced but no larvae were associated with them. The 13 species were reciprocally monophyletic and had minimum interspecific sequence divergences ranging from 7.2–19.5%, higher than the maximum intraspecific sequence divergences (0.6–5.8%). The combination of morphology and molecular data enabled rapid life stage association for alpine Riekoperla species and this method should be used more frequently for other environmentally significant aquatic insects.

Molecular and Ultrastructural Analysis of a Porocephalus sp. (Arthropoda: Pentastomida) Removed from a Rattlesnake

2021 ◽  
Vol Vol 66 (1) (January (1)) ◽  
pp. 1-5
Author(s):  
Jerome Goddard ◽  
Gerald Baker ◽  
Petra Jericke ◽  
Lawrence Birchman ◽  
Ethan Woodward ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Adult Female ◽  
Sequence Data ◽  
Molecular Data ◽  
Ultrastructural Analysis ◽  
Oxidase Subunit ◽  
Data Support ◽  
Partial 18S

Ultrastructural and molecular data are provided from a single adult female pentastomid opportunistically collected from a road-killed rattlesnake in Russell, KS. Ultrastructural data consisted of light and SEM microscopy of the pentastomid and its eggs, while molecular data consisted of partial 18S and 28S ribosomal sequences and a partial cytochrome c oxidase subunit 1 sequence from the same specimen used for SEM. Ultrastructural and molecular data support generic identification of the pentastomid as Porocephalus sp. These molecular data were also used with previously published pentastomid sequence data for a concatenated phylogenetic analysis, which support the current, morphology-based taxonomic placement of the genus.

Mitochondrial DNA replication and transcription are dissociated during embryonic cardiac hypertrophy

1991 ◽  
Vol 261 (6) ◽  
pp. C1091-C1098 ◽  
Author(s):  
J. M. Kennedy ◽  
S. R. Lobacz ◽  
S. W. Kelley
Keyword(s):  
Mitochondrial Dna ◽  
Cytochrome C ◽  
Cardiac Hypertrophy ◽  
Cytochrome C Oxidase ◽  
Oxidase Activity ◽  
Citrate Synthase ◽  
Incubation Temperature ◽  
Mitochondrial Gene ◽  
Gene Replication ◽  
Mitochondrial Dna Copy Number

Cardiac hypertrophy was produced in embryonic chicks by decreasing the incubation temperature from 38 degrees C to 32 degrees C on day 11. Increases in ventricular protein, RNA, and DNA support the cardiac enlargement. Cytochrome-c oxidase activity and citrate synthase activity were depressed in hypothermic ventricles by 63% and 56%, respectively. No significant differences were seen in enzyme activities in pectoralis muscles. The involvement of mitochondrial gene replication and transcription was evaluated using a cDNA clone for the mitochondrially encoded subunit III of cytochrome-c oxidase (CO III). Quantitative slot-blot analysis demonstrated that the relative CO III mRNA concentration was reduced in hypothermic ventricles. In contrast, the relative mitochondrial DNA concentration was increased in hypothermic ventricles. Taken together, these data indicate that a hypothermia-induced decrease in cytochrome-c oxidase activity is associated with a decrease in CO III mRNA, which is not coupled to a decrease in the mitochondrial DNA copy number. This dissociation of mitochondrial gene replication and transcription may provide a useful model for examining the regulation of mitochondrial biogenesis.

DNA barcodes for insect pest identification: a test case with tussock moths (Lepidoptera: Lymantriidae)

2006 ◽  
Vol 36 (2) ◽  
pp. 337-350 ◽  
Author(s):  
Shelley L Ball ◽  
Karen F Armstrong
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Sequence Data ◽  
Mitochondrial Gene ◽  
Insect Pest ◽  
Reference Sequence ◽  
Identification System ◽  
Pest Species ◽  
Dna Barcodes ◽  
Data Set

Reliable and rapid identification of exotic pest species is critical to biosecurity. However, identification of morphologically indistinct specimens, such as immature life stages, that are frequently intercepted at borders is often impossible. Several DNA-based methods have been used for species identification; however, a more universal and anticipatory identification system is needed. Consequently, we tested the ability of DNA "barcodes" to identify species of tussock moths (Lymantriidae), a family containing several important pest species. We sequenced a 617 base pair fragment of the mitochondrial gene cytochrome c oxidase 1 for 20 lymantriid species. We used these, together with other Noctuoidea species sequences from GenBank and the Barcoding of Life Database to create a "profile" or reference sequence data set. We then tested the ability of this profile to provide correct species identifications for 93 additional lymantriid specimens from a data set of mock unknowns. Of the unknowns, 100% were correctly identified by the cytochrome c oxidase 1 profile. Mean interspecific sequence (Kimura 2-parameter) divergence was an order of magnitude greater (14%) than mean intraspecific divergence (<1%). Four species showed deeper genetic divergences among populations. We conclude that DNA barcodes provide a highly accurate means of identifying lymantriid species and show considerable promise as a universal approach to DNA-based identification of pest insects.

The cosmopolitan spider Cryptachaea blattea (Urquhart 1886) (Araneae: Theridiidae): Redescription, including COI sequence, and new synonymy

Zootaxa ◽  
2009 ◽  
Vol 2133 (1) ◽  
pp. 55-63 ◽  
Author(s):  
COR J. VINK ◽  
NADINE DUPÉRRÉ ◽  
PIERRE PAQUIN ◽  
BRIAN M. FITZGERALD ◽  
PHIL J. SIRVID
Keyword(s):  
Mitochondrial Dna ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Dna Sequences ◽  
Mitochondrial Gene ◽  
Junior Synonym ◽  
New Combination ◽  
Morphological Evidence ◽  
Cosmopolitan Species ◽  
New Synonymy

Cryptachaea blattea (Urquhart 1886) new combination, a cosmopolitan species of the spider family Theridiidae, is redescribed, including notes on its distribution and DNA sequences from the mitochondrial gene cytochrome c oxidase subunit 1. Based on morphological evidence and mitochondrial DNA sequences, Cryptachaea acoreensis Berland 1932 is a junior synonym of Cryptachaea blattea.

Reuniting males and females: redescriptions of Nuisiana arboris (Marples 1959) and Cambridgea reinga Forster & Wilton 1973 (Araneae: Desidae, Stiphidiidae)

Zootaxa ◽  
2011 ◽  
Vol 2739 (1) ◽  
pp. 41 ◽  
Author(s):  
COR J. VINK ◽  
BRIAN M. FITZGERALD ◽  
PHIL J. SIRVID ◽  
NADINE DUPÉRRÉ
Keyword(s):  
Mitochondrial Dna ◽  
New Zealand ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Dna Sequences ◽  
Mitochondrial Gene ◽  
Junior Synonym ◽  
Morphological Evidence ◽  
Spider Species ◽  
Males And Females

Two New Zealand endemic spider species, Nuisiana arboris (Marples 1959) (Desidae) and Cambridgea reinga Forster & Wilton 1973 (Stiphidiidae), are redescribed, including notes on their distribution and DNA sequences from the mitochondrial gene cytochrome c oxidase subunit 1. Based on morphological evidence and mitochondrial DNA sequences, Matachia magna Forster 1970 is a junior synonym of Nuisiana arboris, and Nanocambridgea grandis Blest & Vink 2000 is a junior synonym of Cambridgea reinga. Two forms of male morph in C. reinga are recorded.

Four new species of the primitively segmented spider genus Songthela (Mesothelae, Liphistiidae) from Chongqing Municipality, China

Zootaxa ◽  
2022 ◽  
Vol 5091 (4) ◽  
pp. 546-558
Author(s):  
ZHAOYANG CHEN ◽  
FENGXIANG LIU ◽  
DAIQIN LI ◽  
XIN XU
Keyword(s):  
Mitochondrial Dna ◽  
New Species ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Dna Barcode ◽  
Morphological Characters ◽  
Oxidase Subunit ◽  
Chongqing Municipality ◽  
Males And Females

This paper reports four new species of the primitively segmented spider genus Songthela from Chongqing Municipality, China, based on morphological characters of both males and females: S. jinyun sp. nov., S. longbao sp. nov., S. serriformis sp. nov. and S. wangerbao sp. nov. We also provide the GenBank accession codes of mitochondrial DNA barcode gene, cytochrome c oxidase subunit I (COI), for the holotype of four new species for future identification.  

Isostenosmylus ammirabilis sp. nov., a remarkable new species of lance lacewing (Neuroptera: Osmylidae) from the Colombian Andes

Zootaxa ◽  
2020 ◽  
Vol 4803 (3) ◽  
pp. 561-575
Author(s):  
ADRIAN ARDILA-CAMACHO ◽  
CALEB CALIFRE MARTINS ◽  
JORGE ARI NORIEGA
Keyword(s):  
New Species ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Mitochondrial Gene ◽  
Dna Barcode ◽  
Neotropical Region ◽  
Andean Region ◽  
Oxidase Subunit ◽  
Colombian Andes ◽  
South Of Brazil

Isostenosmylus Krüger, 1913 is the richest genus of Osmylidae of the Neotropical region, with 17 described species so far, which are distributed mainly in the Andean region and in the South of Brazil and Paraguay. A new remarkable Colombian species of Isostenosmylus—I. ammirabilis sp. nov.—is herein described and illustrated. DNA barcode of mitochondrial gene cytochrome c oxidase subunit I (COI) for this species is also provided. Taxonomic keys for the genus are updated. 

scholarly journals Molecular analysis of populations of the invasive species Rhynchophorus ferrugineus Olivier, 1790 (Coleoptera: Curculionidae) in the Region of Murcia (Spain)

2017 ◽  
pp. 155-176
Author(s):  
Miguel Lozano-Terol ◽  
María Juliana Rodríguez-García ◽  
José Galián
Keyword(s):  
Invasive Species ◽  
Phylogenetic Analysis ◽  
Mitochondrial Dna ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Molecular Analysis ◽  
Cox1 Gene ◽  
Rhynchophorus Ferrugineus ◽  
Phylogeographic Analysis ◽  
Oxidase Subunit

En este estudio se analizan dos fragmentos del gen de la citocromo c oxidasa subunidad I (COX1) del ADN mitocondrial de 61 individuos del género Rhynchophorus colectados en la Región de Murcia a fin de determinar su procedencia. El análisis filogenético del fragmento 1 de las muestras de la Región de Murcia conjuntamente con las secuencias disponibles en GenBank indica que los individuos corresponden a la especie Rhynchophorus ferrugineus.Las secuencias de Murcia se colapsan en un único haplotipo (H8 mediterráneo) que aparece dentro del clado de R. ferrugineus. De los análisis filogeográficos se infiere que el origen de los individuos de Murcia es Egipto. Adicionalmente, se examinó una región contigua del COX1 (fragmento 2) en la que las secuencias se colapsaron en dos haplotipos. In this research two fragments of the cytochrome c oxidase subunit I (COX1) gene of the mitochondrial DNA were analyzed in 61 individuals of the genus Rhynchophorus collected in the Region of Murcia with the aim of determining their origin. Phylogenetic analysis of fragment 1 of the samples collected in the Region of Murcia together with the available sequences in GenBank, indicated that these individuals correspond to the species R. ferrugineus. Sequences from Murcia collapsed into the H8 Mediterranean haplotype, which cluster into the R. ferrugineus clade. Phylogeographic analysis shows that the origin of the individuals collected in the Region of Murcia is Egypt. Additionally, a contiguous fragment of COX1 (fragment 2) was analyzed and the sequences collapsed into two haplotypes.

scholarly journals Heteroplasmic Point Mutations of Mitochondrial DNA Affecting Subunit I of Cytochrome c Oxidase in Two Patients With Acquired Idiopathic Sideroblastic Anemia

Blood ◽  
1997 ◽  
Vol 90 (12) ◽  
pp. 4961-4972 ◽  
Author(s):  
Norbert Gattermann ◽  
Stefan Retzlaff ◽  
Yan-Ling Wang ◽  
Götz Hofhaus ◽  
Jürgen Heinisch ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mitochondrial Dna ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Respiratory Chain ◽  
Ferric Iron ◽  
Point Mutations ◽  
Polymorphism Analysis ◽  
Sideroblastic Anemia ◽  
Wide Range

Mitochondrial iron overload in acquired idiopathic sideroblastic anemia (AISA) may be attributable to mutations of mitochondrial DNA (mtDNA), because these can cause respiratory chain dysfunction, thereby impairing reduction of ferric iron (Fe3+) to ferrous iron (Fe2+). The reduced form of iron is essential to the last step of mitochondrial heme biosynthesis. It is not yet understood to which part of the respiratory chain the reduction of ferric iron is linked. In two patients with AISA we identified point mutations of mtDNA affecting the same transmembrane helix within subunit I of cytochrome c oxidase (COX I; ie, complex IV of the respiratory chain). The mutations were detected by restriction fragment length polymorphism analysis and temperature gradient gel electrophoresis. One of the mutations involves a T → C transition in nucleotide position 6742, causing an amino acid change from methionine to threonine. The other mutation is a T → C transition at nt 6721, changing isoleucine to threonine. Both amino acids are highly conserved in a wide range of species. Both mutations are heteroplasmic, ie, they establish a mixture of normal and mutated mitochondrial genomes, which is typical of disorders of mtDNA. The mutations were present in bone marrow and whole blood samples, in isolated platelets, and in granulocytes, but appeared to be absent from T and B lymphocytes purified by immunomagnetic bead separation. They were not detected in buccal mucosa cells obtained by mouthwashes and in cultured skin fibroblasts examined in one of the patients. In both patients, this pattern of involvement suggests that the mtDNA mutation occurred in a self-renewing bone marrow stem cell with myeloid determination. Identification of two point mutations with very similar location suggests that cytochrome c oxidase plays an important role in the pathogenesis of AISA. COX may be the physiologic site of iron reduction and transport through the inner mitochondrial membrane.

scholarly journals Mitochondrial Antisense RNA for Cytochrome C Oxidase (MARCO) Can Induce Morphologic Changes and Cell Death in Human Hematopoietic Cell Lines

Blood ◽  
1997 ◽  
Vol 90 (11) ◽  
pp. 4567-4577 ◽  
Author(s):  
Naoki Shirafuji ◽  
Satoshi Takahashi ◽  
Satoru Matsuda ◽  
Shigetaka Asano
Keyword(s):  
Molecular Weight ◽  
Cell Death ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Antisense Rna ◽  
Mitochondrial Gene ◽  
Neutrophilic Granulocytes ◽  
High Molecular Weight Dna ◽  
Tnf Α ◽  
Morphologic Changes

To identify essential molecules capable of inducing terminal morphologic maturation and cell death of myeloid progenitor cells, we isolated cDNA clones by functional expression cloning using a library constructed from all-trans retinoic acid (ATRA)-treated human promyelocytic HL-60 cells. Clones which induced morphologic changes in HL-60 cells from blastic cells to mature neutrophilic granulocytes were selected. The isolated positive cDNA clone was demonstrated to encode an antisense RNA for cytochrome c oxidase/serine tRNA derived from a mitochondrial gene (MARCO). When MARCO was expressed in HL-60 cells with the lac switch system, blastic cell morphology became neutrophilic after 48-hour incubation with IPTG, and cell death was observed after 3 days. Also, high molecular weight DNA fragmentation was observed after 36 hours in culture. Similar results were observed using transformants from human K562 cells and CMK cells. RT-PCR analysis revealed that MARCO was transcribed in both ATRA and TNF-α systems, and also in human blood neutrophilic granulocytes. Following transfection with cytochrome c oxidase expression plasmids, TNF-α–induced high molecular weight DNA fragmentation in U937 cells and HL-60 cells was inhibited in these transformants. These results indicate that maturational changes in hematopoietic cells and the process of cell death may be induced by mitochondrial respiratory insufficiency, and also that the mitochondrial gene MARCO may be used as one of the candidates for gene supplementation therapy for the acute leukemias.

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