Alan Buchanan Wardrop 1921 - 2003

2007 ◽  
Vol 18 (1) ◽  
pp. 113
Author(s):  
Richard E. Williamson ◽  
Huntly G. Higgins ◽  
Bruce A. Stone
Keyword(s):  
Biological Sciences ◽  
Electron Microscopy ◽  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Polarized Light ◽  
Forest Products ◽  
Industrial Research ◽  
X Rays ◽  
Wood Fibres

Alan Buchanan Wardrop was one of Australia's most distinguished students of plant cell wall ultra-structure who made major contributions to our understanding of the structure of secondary walls and of how that structure affected the industrial uses of wood fibres. His work integrated information from observations with polarized light, X-rays and electron microscopy. Joining the Forest Products Division of Australia's Council for Scientific and Industrial Research in 1945, he became Foundation Professor of Biological Sciences at La Trobe University, Melbourne, in 1965.

scholarly journals A Simple Technique For The Removal Of Plant Cell Protoplasm To Facilitate Scanning : Electron Microscopy Of Fungal Haustoria And Plant Cell Wall Features

2001 ◽  
Vol 9 (3) ◽  
pp. 14-15 ◽  
Author(s):  
B. A. Richardson ◽  
C. W. Mims
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Wall ◽  
Plant Cell ◽  
Host Plants ◽  
Plant Cell Wall ◽  
Simple Technique ◽  
Plant Cells ◽  
Host Cell Wall ◽  
Scanning Electron

Several years ago Honegger (1985) described a simple technique for removing plant cell protoplasm in order to reveal details of interfaces between plant cells and fungal structures. This technique involves the use of Ariel a commercially available washing powder (Proctor and Gamble) containing a Bacillus substilis derived protease. We since have used this technique with excellent results to examine not only the morphology of fungal haustoria inside leaf cells of various host plants but also features of the inner surface of the host cell wall with scanning electron microscopy (SEM). Here we describe the procedure we have used to prepare samples for study and provide examples of the types of images we have obtained from our samples.

scholarly journals Characterisation of the Effect of the Spatial Organisation of Hemicellulases on the Hydrolysis of Plant Biomass Polymer

2020 ◽  
Vol 21 (12) ◽  
pp. 4360
Author(s):  
Thomas Enjalbert ◽  
Marion De La Mare ◽  
Pierre Roblin ◽  
Louise Badruna ◽  
Thierry Vernet ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Plant Biomass ◽  
Reducing Sugar ◽  
Intrinsic Activity ◽  
X Rays ◽  
Spatial Organisation ◽  
The Impact ◽  
Hydrolysis Of

Synergism between enzymes is of crucial importance in cell metabolism. This synergism occurs often through a spatial organisation favouring proximity and substrate channelling. In this context, we developed a strategy for evaluating the impact of the geometry between two enzymes involved in nature in the recycling of the carbon derived from plant cell wall polymers. By using an innovative covalent association process using two protein fragments, Jo and In, we produced two bi-modular chimeric complexes connecting a xylanase and a xylosidase, involved in the deconstruction of xylose-based plant cell wall polymer. We first show that the intrinsic activity of the individual enzymes was preserved. Small Angle X-rays Scattering (SAXS) analysis of the complexes highlighted two different spatial organisations in solution, affecting both the distance between the enzymes (53 Å and 28 Å) and the distance between the catalytic pockets (94 Å and 75 Å). Reducing sugar and HPAEC-PAD analysis revealed different behaviour regarding the hydrolysis of Beechwood xylan. After 24 h of hydrolysis, one complex was able to release a higher amount of reducing sugar compare to the free enzymes (i.e., 15,640 and 14,549 µM of equivalent xylose, respectively). However, more interestingly, the two complexes were able to release variable percentages of xylooligosaccharides compared to the free enzymes. The structure of the complexes revealed some putative steric hindrance, which impacted both enzymatic efficiency and the product profile. This report shows that controlling the spatial geometry between two enzymes would help to better investigate synergism effect within complex multi-enzymatic machinery and control the final product.

Origin of the Inner Intine in Pollen of Canna

1974 ◽  
Vol 32 ◽  
pp. 84-85 ◽  
Author(s):  
John R. Rowley ◽  
John J. Skvarla
Keyword(s):  
Electron Microscopy ◽  
Cell Wall ◽  
Cell Surface ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Amorphous Matrix ◽  
Light And Electron Microscopy ◽  
Pollen Wall ◽  
Cytoplasmic Surface ◽  
Canna Generalis

Observations of development in the nonaperaturate pollen of Canna generalis Bailey, based upon light and electron microscopy, indicate that the inner intine of this species is initiated by plasmalemmasomes originating at widely separated areas of the cell surface.The intine is that part of the pollen wall located between the sporopollenin exine and the cytoplasmic surface. It is often interbedded with the exine but is not itself composed of sporopollenin. In appearance and composition, the intine is comparable with the primary plant cell wall and has been characterized by Roland as an amorphous matrix of pectins with infrequent microfibrils.

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