scholarly journals Ultrastructure and Topochemistry of Plant Cell Wall by Transmission Electron Microscopy

10.5772/60752 ◽  
2015 ◽  
Author(s):  
Xia Zhou ◽  
Dayong Ding ◽  
Jing Ma ◽  
Zhe Ji ◽  
Xun Zhang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Transmission Electron

scholarly journals Chemical composition, anatomy, lignin distribution, and cell wall structure of Malaysian plant waste fibers

BioResources ◽  
2006 ◽  
Vol 1 (2) ◽  
pp. 220-232 ◽  
Author(s):  
H. P. S. Abdul Khalil ◽  
M. Siti Alwani ◽  
A. K. Mohd Omar
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Cell Wall ◽  
Chemical Composition ◽  
Cell Wall Structure ◽  
Wall Structure ◽  
Anatomical Characteristics ◽  
Lignin Distribution ◽  
Transmission Electron ◽  
Palm Frond

The chemical composition, anatomical characteristics, lignin distribution, and cell wall structure of oil palm frond (OPF), coconut (COIR), pine-apple leaf (PALF), and banana stem (BS) fibers were analyzed. The chemical composition of fiber was analyzed according to TAPPI Methods. Light microscopy (LM) and transmission electron microscopy (TEM) were used to observe and determine the cell wall structure and lignin distribution of various agro-waste fibers. The results revealed differences in anatomical characteristics, lignin distributions, and cell wall structure of the different types of fibers investigated. Nevertheless, transmission electron microscopy (TEM) micrographs have confirmed that the well wall structure, in each case, could be described in terms of a classical cell wall structure, consisting of primary (P) and secondary (S 1 , S 2 , and S 3 ) layers.

Ultrastructure of hyperparasitism of Coniothyrium minitans on sclerotia of Sclerotinia sclerotiorum

1987 ◽  
Vol 65 (12) ◽  
pp. 2483-2489 ◽  
Author(s):  
H. C. Huang ◽  
E. G. Kokko
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Cell Wall ◽  
Sclerotinia Sclerotiorum ◽  
Cell Walls ◽  
Chemical Activity ◽  
Coniothyrium Minitans ◽  
Medullary Tissue ◽  
Transmission Electron

Transmission electron microscopy revealed that hyphae of the hyperparasite Coniothyrium minitans invade sclerotia of Sclerotinia sclerotiorum, resulting in the destruction and disintegration of the sclerotium tissues. The dark-pigmented rind tissue is more resistant to invasion by the hyperparasite than the unpigmented cortical and medullary tissues. Evidence from cell wall etching at the penetration site suggests that chemical activity is required for hyphae of C. minitans to penetrate the thick, melanized rind walls. The medullary tissue infected by C. minitans shows signs of plasmolysis, aggregation, and vacuolization of cytoplasm and dissolution of the cell walls. While most of the hyphal cells of C. minitans in the infected sclerotium tissue are normal, some younger hyphal cells in the rind tissue were lysed and devoid of normal contents.

Poly(β-hydroxybutyrate) extrusion from pleomorphic cells ofAzotobacter vinelandiiUWD

1995 ◽  
Vol 41 (13) ◽  
pp. 22-31 ◽  
Author(s):  
William J. Page ◽  
Luis D'elia ◽  
Richard Sherburne ◽  
Lori L. Graham
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Cell Wall ◽  
Azotobacter Vinelandii ◽  
Intact Cell ◽  
Viable Cell ◽  
Transmission Electron ◽  
Polymer Expansion ◽  
Chemical Dehydration ◽  
Pleomorphic Cells

Azotobacter vinelandii UWD cells fill with up to 80% (per dry mass) poly(β-hydroxybutyrate) (PHB) after 24 h growth in medium containing sugars and fish peptone. However, peptones were not usually added to Azotobacter culture as they induced pleomorphism and compromised cell wall strength. This study examines the morphology of these PHB-producing pleomorphic cells in the transmission electron microscope. PHB-producing cells incubated for 18–24 h were most frequently 2–3 μm diameter spheres containing up to 20 PHB inclusions/cross section, or a calculated ≈ 100 inclusions/cell volume. These inclusions tended to be of small size (≈ 0.5 μm diameter) and became fewer and larger in older cells. The most striking feature of these pleomorphic cells was the apparent extrusion of polymer from the cells. It is unlikely that PHB extrusion is an active process from a viable cell as there was considerable cell wall damage at the point of polymer extrusion. The results suggest that the extrusion of PHB may be the result of polymer expansion, caused by the dehydration of the specimen for transmission electron microscopy, coupled with the inability of the pleomorphic cell wall to retain the expanding polymer. Thus, freeze-substituted sections of similar cells that were prepared without chemical dehydration did not extrude PHB. However, lysed cells prepared for transmission electron microscopy by chemical dehydration also did not extrude PHB, which suggests differences in the fluidity of the PHB in intact cell inclusions and lysed cell granules.Key words: poly(β-hydroxybutyrate), inclusions, polymer expansion, dehydration artifact.

Transmission Electron Microscopy of Unfrozen and Frozen/Thawed Cells of Listeria monocytogenes Treated With Lipase and Lysozyme

1992 ◽  
Vol 55 (9) ◽  
pp. 687-696 ◽  
Author(s):  
SOUZAN E. EL-KEST ◽  
ELMER H. MARTH
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Cell Wall ◽  
Listeria Monocytogenes ◽  
Storage Time ◽  
Frozen Storage ◽  
Activity Type ◽  
Protoplast Formation ◽  
Transmission Electron ◽  
Three Stages

Unfrozen cells of Listeria monocytogenes typically contained no preplasma space exterior to the plasma membrane (PM) when viewed by transmission electron microscopy. Cells of L. monocytogenes strains Scott A, V7, and California (CA), after freezing and frozen storage, exhibited one or more of the following when viewed with transmission electron microscopy: (a) retraction of cytoplasm and infolding of the PM to form mesosomes, (b) extra-and intracellular rupture of the cell wall (CW), (c) formation of intracellular “bubbles,” and (d) damage to the CW and PM that could have resulted from autolysin activity. Type and degree of effect depended on frozen storage time and strain of L. monocytogenes. Lysozyme treatment of unfrozen or frozen/stored (19 d)/thawed cells of strains Scott A, V7, and CA resulted in protoplast formation and damage to the CW. Three stages of protoplast formation were observed when cells of strain CA were frozen, stored 2 weeks, thawed, and treated with lysozyme. More damage to the CW and PM occurred when frozen storage time was extended for up to 6 weeks before treatment with lysozyme. Lipase and lysozyme treatment of unfrozen or frozen/stored (19 d)/thawed cells of strain Scott A resulted in protoplast formation with some damage to the PM and irregularity in shape of cells. Damage to the PM increased with increasing frozen storage time for up to 6 weeks. Some cells of strain CA resisted freezing, frozen storage for 6 weeks, thawing, and treatment with lipase and lysozyme.

scholarly journals Swelling of Valonia cellulose microfibrils in amine oxide systems

2008 ◽  
Vol 86 (6) ◽  
pp. 520-524 ◽  
Author(s):  
Pierre Noé ◽  
Henri Chanzy
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Cell Wall ◽  
Electron Diffraction ◽  
Amine Oxide ◽  
Cellulose Microfibrils ◽  
Electron Diffraction Analysis ◽  
Oxide Systems ◽  
Transmission Electron ◽  
Valonia Ventricosa

Cellulose microfibrils from Valonia ventricosa cell-wall fragments were immersed into molten N-methylmorpholine-N-oxide monohydrate (NMMO·H2O), stabilized with n-propyl gallate and kept at 80 °C. The resulting ultrastructural modifications, which were followed by transmission electron microscopy and electron diffraction analysis, showed that within minutes the solvent slowly penetrated inside the crystalline microfibrils and progressed as a wedge in between the cellulose chains without cutting them. Prior to dissolution, a longitudinal subfibrillation of the initial microfibrils occurred, leading to the observation of highly swollen microfibrils, which could reach diameters up to three times larger than those of the initial samples. This mode of swelling is compared with those occurring in other systems, where the intracrystalline swelling of cellulose has been described at the ultrastructural level.Key words: cellulose swelling, Valonia cellulose, N-methylmorpholine-N-oxide.

scholarly journals A Simple Technique For The Removal Of Plant Cell Protoplasm To Facilitate Scanning : Electron Microscopy Of Fungal Haustoria And Plant Cell Wall Features

2001 ◽  
Vol 9 (3) ◽  
pp. 14-15 ◽  
Author(s):  
B. A. Richardson ◽  
C. W. Mims
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Wall ◽  
Plant Cell ◽  
Host Plants ◽  
Plant Cell Wall ◽  
Simple Technique ◽  
Plant Cells ◽  
Host Cell Wall ◽  
Scanning Electron

Several years ago Honegger (1985) described a simple technique for removing plant cell protoplasm in order to reveal details of interfaces between plant cells and fungal structures. This technique involves the use of Ariel a commercially available washing powder (Proctor and Gamble) containing a Bacillus substilis derived protease. We since have used this technique with excellent results to examine not only the morphology of fungal haustoria inside leaf cells of various host plants but also features of the inner surface of the host cell wall with scanning electron microscopy (SEM). Here we describe the procedure we have used to prepare samples for study and provide examples of the types of images we have obtained from our samples.

scholarly journals RrgA and RrgB Are Components of a Multisubunit Pilus Encoded by the Streptococcus pneumoniae rlrA Pathogenicity Islet

2006 ◽  
Vol 74 (4) ◽  
pp. 2453-2456 ◽  
Author(s):  
Julianna LeMieux ◽  
David L. Hava ◽  
Alan Basset ◽  
Andrew Camilli
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Cell Wall ◽  
Streptococcus Pneumoniae ◽  
Western Blotting ◽  
Surface Proteins ◽  
Domain Swapping ◽  
Cell Fractionation ◽  
Sorting Signal ◽  
Transmission Electron

ABSTRACT The rlrA pathogenicity islet in Streptococcus pneumoniae TIGR4 encodes three surface proteins, RrgA, RrgB, and RrgC, and three sortase enzymes. Using transmission electron microscopy, cell fractionation, cell wall sorting signal domain swapping, and Western blotting, we show that RrgA and RrgB are incorporated into a multisubunit pilus in S. pneumoniae.

scholarly journals A Three-Dimensional Model of the Yeast Transmembrane Sensor Wsc1 Obtained by SMA-Based Detergent-Free Purification and Transmission Electron Microscopy

2021 ◽  
Vol 7 (2) ◽  
pp. 118
Author(s):  
Natalia Voskoboynikova ◽  
Maria Karlova ◽  
Rainer Kurre ◽  
Armen Y. Mulkidjanian ◽  
Konstantin V. Shaitan ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Cell Wall ◽  
Transmembrane Domain ◽  
Three Dimensional ◽  
Water Soluble ◽  
Dimensional Model ◽  
Three Dimensional Model ◽  
Water Soluble Polymer ◽  
Transmission Electron

The cell wall sensor Wsc1 belongs to a small family of transmembrane proteins, which are crucial to sustain cell integrity in yeast and other fungi. Wsc1 acts as a mechanosensor of the cell wall integrity (CWI) signal transduction pathway which responds to external stresses. Here we report on the purification of Wsc1 by its trapping in water-soluble polymer-stabilized lipid nanoparticles, obtained with an amphipathic styrene-maleic acid (SMA) copolymer. The latter was employed to transfer tagged sensors from their native yeast membranes into SMA/lipid particles (SMALPs), which allows their purification in a functional state, i.e., avoiding denaturation. The SMALPs composition was characterized by fluorescence correlation spectroscopy, followed by two-dimensional image acquisition from single particle transmission electron microscopy to build a three-dimensional model of the sensor. The latter confirms that Wsc1 consists of a large extracellular domain connected to a smaller intracellular part by a single transmembrane domain, which is embedded within the hydrophobic moiety of the lipid bilayer. The successful extraction of a sensor from the yeast plasma membrane by a detergent-free procedure into a native-like membrane environment provides new prospects for in vitro structural and functional studies of yeast plasma proteins which are likely to be applicable to other fungi, including plant and human pathogens.

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