Uncovering early response to drought by proteomic, physiological and biochemical changes in the almond × peach rootstock ‘Garnem'

Beatriz Bielsa; María Á. Sanz; María J. Rubio-Cabetas

doi:10.1071/fp19050

Uncovering early response to drought by proteomic, physiological and biochemical changes in the almond × peach rootstock ‘Garnem'

Functional Plant Biology ◽

10.1071/fp19050 ◽

2019 ◽

Vol 46 (11) ◽

pp. 994

Author(s):

Beatriz Bielsa ◽

María Á. Sanz ◽

María J. Rubio-Cabetas

Keyword(s):

Drought Stress ◽

Protein Function ◽

Proteomic Analysis ◽

Biochemical Analysis ◽

Stomatal Closure ◽

Early Response ◽

Short Term ◽

Water Losses ◽

Dehydration Avoidance ◽

Physiological And Biochemical

Drought affects growth and metabolism in plants. To investigate the changes in root protein function involved in the early response to drought stress, a proteomic analysis in combination to a physiological and biochemical analysis was performed in plants of ‘Garnem’, an almond × peach hybrid rootstock, subjected to short-term drought stress. Abscisic acid (ABA) accumulation levels increased during the drought exposure, which induced stomatal closure, and thus, minimised water losses. These effects were reflected in stomatal conductance and leaf water potential levels. However, ‘Garnem’ was able to balance water content and maintain an osmotic adjustment in cell membranes, suggesting a dehydration avoidance strategy. The proteomic analysis revealed significant abundance changes in 29 and 24 spots after 2 and 24 h of drought stress respectively. Out of these, 15 proteins were identified by LC-ESI-MS/MS. The abundance changes of these proteins suggest the influence in drought-responsive mechanisms present in ‘Garnem’, allowing its adaptation to drought conditions. Overall, our study improves existing knowledge on the root proteomic changes in the early response to drought. This will lead to a better understanding of dehydration avoidance and tolerance strategies, and finally, help in new drought-tolerance breeding approaches.

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Climate change due to heat and drought stress can alter the physiology of Maratheftiko local Cyprian grapevine variety

Journal of Water and Climate Change ◽

10.2166/wcc.2018.226 ◽

2018 ◽

Vol 9 (4) ◽

pp. 715-727 ◽

Cited By ~ 4

Author(s):

Antonios Chrysargyris ◽

Panayiota Xylia ◽

Omiros Antoniou ◽

Nikos Tzortzakis

Keyword(s):

Climate Change ◽

Drought Stress ◽

Control Treatment ◽

Stress Exposure ◽

Short Term ◽

Stress Markers ◽

P Content ◽

Moderate Drought ◽

Leaf Photosynthetic Rate ◽

Physiological And Biochemical

Abstract The effect of climate change (CC) on viticulture in Europe is of great concern and several international and indigenous grapevine cultivars have been examined for their adaptation to CC. In this study we focused on the short-term effects of light and moderate drought stress (DS) as well as heat stress (HS) on physiological and biochemical stress markers in Maratheftiko cultivar. We showed that leaf photosynthetic rate was decreased with DS and HS after 8 and 20 days. The leaf stomatal conductance was decreased in the case of DS after 8 days, while no differences could be found due to HS. Total phenols and flavonoids content and antioxidant capacity (FRAP and ABTS) were increased and seemed to be dependent on the relevant DS, HS and the period of stress exposure. Chlorophyll fluorescence was decreased in 50% volumetric water content (VWC) after 8 days of DS compared with the 100% VWC (control treatment). Leaf K and P content increased in moderate (50% VWC) irrigation stress and HS. Leaf hydrogen peroxide and lipid peroxidation increased after 8 days of DS, and this resulted in the increase of antioxidant enzymes activity. Overall, Maratheftiko performance against environmental stresses is related more to short-term DS than HS.

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Isoprene emission is not temperature-dependent during and after severe drought-stress: a physiological and biochemical analysis

The Plant Journal ◽

10.1111/j.1365-313x.2008.03538.x ◽

2008 ◽

Vol 55 (4) ◽

pp. 687-697 ◽

Cited By ~ 116

Author(s):

Alessio Fortunati ◽

Csengele Barta ◽

Federico Brilli ◽

Mauro Centritto ◽

Ina Zimmer ◽

...

Keyword(s):

Drought Stress ◽

Biochemical Analysis ◽

Severe Drought ◽

Temperature Dependent ◽

Isoprene Emission ◽

Physiological And Biochemical

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Proteomic Analysis of the Cyanobacterium Synechococcus cedrorum IU 1191 Under Short Term NaCl Exposure

Current Proteomics ◽

10.2174/157016461202150903112500 ◽

2015 ◽

Vol 12 (2) ◽

pp. 87-95

Author(s):

Durgesh Gupta ◽

Ragini Gothalwal ◽

Santosh Bhargava

Keyword(s):

Proteomic Analysis ◽

Short Term

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Morphological, Physiological and Biochemical Responses of Poplar Plants to Drought Stress

Journal of AgriSearch ◽

10.21921/jas.5.3.7 ◽

2018 ◽

Vol 5 (03) ◽

Author(s):

ARADHNA KUMARI ◽

IM KHAN ◽

ANIL KUMAR SINGH ◽

SANTOSH KUMAR SINGH

Keyword(s):

Water Stress ◽

Drought Stress ◽

Biochemical Parameters ◽

Sugar Content ◽

Soluble Sugar ◽

Field Capacity ◽

Drought Event ◽

Total Biomass ◽

Biochemical Responses ◽

Physiological And Biochemical

Poplar clone Kranti was selected to assess the morphological, physiological and biochemical responses under drought at different levels of water stress, as it is a common clone used to be grown in Uttarakhand for making paper and plywood. The cuttings of Populus deltoides L. (clone Kranti) were exposed to four different watering regimes (100, 75, 50 and 25% of the field capacity) and changes in physiological and biochemical parameters related with drought tolerance were recorded. Alterations in physiological (i.e. decrease in relative water content) and biochemical parameters (i.e. increase in proline and soluble sugar content and build-up of malondialdehyde by-products) occurred in all the three levels of water stress, although drought represented the major determinant. Drought treatments (75%, 50% and 25% FC) decreased plant height, radial stem diameter, harvest index, total biomass content and RWC in all the three watering regimes compared to control (100% FC). Biochemical parameters like proline, soluble sugar and MDA content increased with severity and duration of stress, which helped plants to survive under severe stress. It was analyzed that for better wood yield poplar seedlings should avail either optimum amount of water (amount nearly equal to field capacity of soil) or maximum withdrawal up to 75% of field capacity up to seedling establishment period (60 days). Furthermore, this study manifested that acclimation to drought stress is related with the rapidity, severity, and duration of the drought event of the poplar species.

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Arabidopsis MADS-box factor AGL16 negatively regulates drought resistance via stomatal density and stomatal movement

Journal of Experimental Botany ◽

10.1093/jxb/eraa303 ◽

2020 ◽

Vol 71 (19) ◽

pp. 6092-6106 ◽

Cited By ~ 3

Author(s):

Ping-Xia Zhao ◽

Zi-Qing Miao ◽

Jing Zhang ◽

Si-Yan Chen ◽

Qian-Qian Liu ◽

...

Keyword(s):

Drought Stress ◽

Drought Resistance ◽

Molecular Mechanisms ◽

Stomatal Density ◽

Stomatal Closure ◽

Negative Regulator ◽

Stomatal Development ◽

Mads Box ◽

Mobility Shift ◽

Aba Metabolism

Abstract Drought is one of the most important environmental factors limiting plant growth and productivity. The molecular mechanisms underlying plant drought resistance are complex and not yet fully understood. Here, we show that the Arabidopsis MADS-box transcription factor AGL16 acts as a negative regulator in drought resistance by regulating stomatal density and movement. Loss-of-AGL16 mutants were more resistant to drought stress and had higher relative water content, which was attributed to lower leaf stomatal density and more sensitive stomatal closure due to higher leaf ABA levels compared with the wild type. AGL16-overexpressing lines displayed the opposite phenotypes. AGL16 is preferentially expressed in guard cells and down-regulated in response to drought stress. The expression of CYP707A3 and AAO3 in ABA metabolism and SDD1 in stomatal development was altered in agl16 and overexpression lines, making them potential targets of AGL16. Using chromatin immunoprecipitation, transient transactivation, yeast one-hybrid, and electrophoretic mobility shift assays, we demonstrated that AGL16 was able to bind the CArG motifs in the promoters of the CYP707A3, AAO3, and SDD1 and regulate their transcription, leading to altered leaf stomatal density and ABA levels. Taking our findings together, AGL16 acts as a negative regulator of drought resistance by modulating leaf stomatal density and ABA accumulation.

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Evaluation of leaf physiological and biochemical traits during senescence of the wheat core collection in the southwest of China

Canadian Journal of Plant Science ◽

10.4141/cjps06018 ◽

2008 ◽

Vol 88 (2) ◽

pp. 331-337 ◽

Cited By ~ 2

Author(s):

Kai Shu ◽

Pei G Luo ◽

Huai Y Zhang ◽

Ben J Yan ◽

Jie Zhang ◽

...

Keyword(s):

Core Collection ◽

Biochemical Analysis ◽

Southwest China ◽

Heading Date ◽

Leaf Traits ◽

Triticum Aestivum L ◽

Malondialdehyde Content ◽

Good Potential ◽

Chinese Wheat ◽

Physiological And Biochemical

Agronomical, physiological, and biochemical analysis of the Chinese wheat (Triticum aestivum L.) core collection revealed abundant variation in the indices examined, both within and between ecotype regions. The genotypes from the Yellow-Huai Rivers (YH) region, the Middle and lower reaches of the Yangtze River (MY) region and the Southwest China (SWC) region showed good adaptation as measured by heading date, malondialdehyde content (MDA), and superoxide dismutase (SOD) and catalase (CAT) activities and would have good potential for wheat improvement in SWC. Key words: Adaptability, core collection, diversity, leaf traits, southwest China, wheat

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Development of Composite Indicators of Cyclical Response in Business Surveys

Voprosy statistiki ◽

10.34023/2313-6383-2021-28-2-24-41 ◽

2021 ◽

Vol 28 (2) ◽

pp. 24-41

Author(s):

L. A. Kitrar ◽

T. M. Lipkind

Keyword(s):

Large Scale ◽

Integrated Approach ◽

Early Response ◽

Business Environment ◽

Economic Behavior ◽

Retail Trade ◽

Composite Indicators ◽

Business Activity ◽

Short Term ◽

Current Output

The article proposes a new set of composite indicators-predictors in business tendency surveys, which allow identifying early information signals of a cyclical nature in the economic behavior of business agents. The main criterion for the efficiency of such indicators is their sensitivity to a cyclical pattern and changes in the dynamics of statistical referents. Property such as a statistically significant lead in time series or earlier publication allows them to be combined into indicators of early response. The composite Business Activity Indicator (BAI) in the basic sectors of the Russian economy is calculated by the authors for the first time based on the results of regular (monthly and quarterly) business surveys of Rosstat for 1998–2020 with a large-scale coverage of sampling units. In 2020, the number of survey respondents averaged about 20,000 organizations of all sizes. The index reflects the «common» profile in the dynamics of short-term fluctuations of the key parameters of the economic environment, which consists of the «balances of opinions» of respondents to the questions unified for all sectoral surveys and connected with the reference quantitative statistics with cross-correlation coefficients that are statistically significantly different from zero, with a lead at least one quarter. This is its main difference from the well-known indices of economic sentiment and entrepreneurial confidence. The main components of the BAI are the new composite indices of real demand, current output, real employment, total profits and economic situation. They aggregate the relevant «order» statistics for the basic sectors of the national economy, including the main kinds of industrial activities, retail trade, construction, and services.The article provides a methodological substantiation and an extended procedure for identifying the BAI components; their composition is formed for the entire set of retrospective results of business tendency monitoring in Russia. A new Aggregate Economic Vulnerability Indicator with a counterdirectional profile and varying degrees of symmetry of its dynamics relative to the short-term movement of the BAI is being introduced as the main limitation of business activity. Proactive monitoring of emerging vulnerabilities in the business environment is necessary to warn their large-scale accumulation, prevent the risks of economic downturns and ensure the highest possible macroeconomic stability. This integrated approach makes it possible to determine the novelty of the proposed measurements of short-term cyclical fluctuations in economic development.

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Short-term transcriptomic responses of Populus euphratica roots and leaves to drought stress

Journal of Forestry Research ◽

10.1007/s11676-020-01123-9 ◽

2020 ◽

Author(s):

Peipei Jiao ◽

Zhihua Wu ◽

Xu Wang ◽

Zhenbo Jiang ◽

Yanqin Wang ◽

...

Keyword(s):

Drought Stress ◽

Populus Euphratica ◽

Short Term ◽

Transcriptomic Responses

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Proteomic analysis of haem-binding protein from Arabidopsis thaliana and Cyanidioschyzon merolae

Philosophical Transactions of the Royal Society B Biological Sciences ◽

10.1098/rstb.2019.0488 ◽

2020 ◽

Vol 375 (1801) ◽

pp. 20190488 ◽

Cited By ~ 1

Author(s):

Takayuki Shimizu ◽

Rintaro Yasuda ◽

Yui Mukai ◽

Ryo Tanoue ◽

Tomohiro Shimada ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Proteomic Analysis ◽

High Performance ◽

Biochemical Analysis ◽

Cyanidioschyzon Merolae ◽

Genes Expression ◽

Retrograde Signalling ◽

Coordinated Expression ◽

Retrograde Signal ◽

Affinity Beads

Chloroplast biogenesis involves the coordinated expression of the plastid and nuclear genomes, requiring information to be sent from the nucleus to the developing chloroplasts and vice versa. Although it is well known how the nucleus controls chloroplast development, it is still poorly understood how the plastid communicates with the nucleus. Currently, haem is proposed as a plastid-to-nucleus (retrograde) signal that is involved in various physiological regulations, such as photosynthesis-associated nuclear genes expression and cell cycle in plants and algae. However, components that transduce haem-dependent signalling are still unidentified. In this study, by using haem-immobilized high-performance affinity beads, we performed proteomic analysis of haem-binding proteins from Arabidopsis thaliana and Cyanidioschyzon merolae . Most of the identified proteins were non-canonical haemoproteins localized in various organelles. Interestingly, half of the identified proteins were nucleus proteins, some of them have a similar function or localization in either or both organisms. Following biochemical analysis of selective proteins demonstrated haem binding. This study firstly demonstrates that nucleus proteins in plant and algae show haem-binding properties. This article is part of the theme issue ‘Retrograde signalling from endosymbiotic organelles’.

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