Short-term temperature dependency of the photosynthetic and PSII photochemical responses to photon flux density of leaves of Vitis vinifera cv. Shiraz vines grown in field conditions with and without fruit

2019 ◽  
Vol 46 (7) ◽  
pp. 634 ◽  
Author(s):  
Dennis H. Greer
Keyword(s):  
Maximum Efficiency ◽  
Photon Flux ◽  
Photochemical Quenching ◽  
Photon Flux Density ◽  
Temperature Dependent ◽  
Psii Photochemistry ◽  
Acclimation Response ◽  
Summer Temperatures ◽  
Non Photochemical Quenching ◽  
The Impact

Shiraz vines grown outdoors with and without a crop load were used to determine photosynthetic and chlorophyll fluorescence responses to light across a range of leaf temperatures to evaluate the impact of presence/absence of a sink on these responses. Results indicate maximum rates of photosynthesis and light saturation in fruiting vines were biased towards higher temperatures whereas these processes in vegetative vines were biased towards lower temperatures. The maximum efficiency of PSII photochemistry was similarly biased, with higher efficiency for the vegetative vines below 30°C and a higher efficiency for the fruiting vines above. The quantum efficiency of PSII electron transport was generally higher across all temperatures in the fruiting compared with vegetative vines. Photochemical quenching was not sensitive to temperature in fruiting vines but strongly so in vegetative vines, with an optimum at 30°C and marked increases in photochemical quenching at other temperatures. Non-photochemical quenching was not strongly temperature dependent, but there were marked increases in both treatments at 45°C, consistent with marked decreases in assimilation. These results suggest demand for assimilates in fruiting vines induced an acclimation response to high summer temperatures to enhance assimilate supply and this was underpinned by comparable shifts in PSII photochemistry.

Photon flux density and temperature-dependent responses of photosynthesis and photosystem II performance of apple leaves grown in field conditions

2015 ◽  
Vol 42 (8) ◽  
pp. 782 ◽  
Author(s):  
Dennis H. Greer
Keyword(s):  
Electron Transport ◽  
Flux Density ◽  
Leaf Temperature ◽  
Field Conditions ◽  
Photon Flux ◽  
Photochemical Quenching ◽  
Photon Flux Density ◽  
Temperature Dependent ◽  
Light Responses ◽  
Non Photochemical Quenching

The process of photosynthesis depends on the light, and is modulated by leaf temperature and their interaction is important to understand how climate affects photosynthesis. Photosynthetic and PSII light responses at a range of leaf temperatures were measured on leaves of apple (Malus domestica Borkh. cv. Red Gala) trees growing in field conditions. The objective was to assess the interaction between photon flux density (PFD) and temperature on these processes. Results showed leaf temperature strongly modulated the PFD-dependent response of photosynthesis and PSII performance. An interaction on photosynthesis occurred, with temperature affecting saturated rates as well as PFDs saturating photosynthesis. The efficiency of PSII electron transport (operating and maximum in light) universally declined with increasing PFD but temperature strongly influenced the response. Rates of PSII electron transport at saturating PFDs were affected by temperatures. Both photochemical quenching and non-photochemical quenching also responded strongly to temperature but at high PFDs, photochemical quenching increased linearly with decreasing temperatures while non-photochemical quenching increased curvilinearly with increasing temperatures. Modelling revealed changes in photosynthesis were positively correlated with rates of electron transport. These results greatly enhance our understanding of photosynthesis and the underpinning processes and their responses to temperature and PFD.

Responses of Rainforest Understorey Plants to Excess Light during Sunflecks

1997 ◽  
Vol 24 (1) ◽  
pp. 17 ◽  
Author(s):  
Jenny R. Watling ◽  
Sharon A. Robinson ◽  
Ian E. Woodrow ◽  
C. Barry Osmond
Keyword(s):  
Tropical Rainforest ◽  
Photon Flux ◽  
Photochemical Quenching ◽  
Photon Flux Density ◽  
Leaf Angle ◽  
Low Light ◽  
Fourth Species ◽  
Excess Light ◽  
Understorey Plants ◽  
Non Photochemical Quenching

Responses of Alocasia macrorrhiza (L.) G. Don, Castanospora alphandii (F. Muell.) F. Muell. and Alpinia hylandii R. Smith, growing in a tropical rainforest understorey, to excess light during sunflecks were investigated using chlorophyll fluorescence techniques and by analysing xanthophyll cycle activity. A fourth species, the pioneerOmalanthus novo-guineensis (Warb.) Schum., growing in a small gap, was also studied. In all three understorey species there were large and rapid decreases in the proportion of open Photosystem II (PSII) centres, as indicated by qP, on illumination with saturating light and a concurrent increase in non-photochemical quenching. qP remained low (< 0.4) throughout the period of illumination (~15 min), although it did increase gradually, probably reflecting photosynthetic induction. Sustained declines (up to 120 min) in quantum yield, indicated by Fv/Fm, occurred in all three understorey species following exposure to saturating Photon flux density (PFD) during sunflecks. When ?PSII was monitored during sunflecks it was found to be very sensitive to changes in PFD, declining rapidly with even modest rises in the latter. There was rapid and continuing net conversion of violaxanthin (V) to antheraxanthin plus zeaxanthin (A+Z) on exposure of A. macrorrhiza and C. alphandii to saturating sunflecks. On returning to low light A. macrorrhiza retained its high levels of A+Z for up to 60 min, while C. alphandii rapidly converted back to V. O. novo- guineensis responded to high light by changing its leaf angle to reduce interception and showed no indication of photoinhibition during or after exposure.

A Chlorophyll-Less Barley Mutant “NYB” Is Insensitive to Water Stress

2007 ◽  
Vol 62 (5-6) ◽  
pp. 403-409 ◽  
Author(s):  
Shu Yuan ◽  
Wen-Juan Liu ◽  
Tao Lei ◽  
Ming-Hua Luo ◽  
Jun-Bo Du ◽  
...  
Keyword(s):  
Water Stress ◽  
Maximum Efficiency ◽  
Photochemical Quenching ◽  
Wild Type ◽  
Energy Capture ◽  
Barley Mutant ◽  
Core Proteins ◽  
Psii Photochemistry ◽  
Non Photochemical Quenching ◽  
Quantum Yield Of Psii

“NYB” is a chlorophyll-less barley mutant, which grows relatively slow and unhealthily. The effects of water stress on photosystem II (PSII) of NYB and its wild type (WT) were investigated. Unexpected results indicated that the mutant was more resistant to water stress, because: PSII core proteins D1, D2 and LHCII declined more in WT than in NYB under water stress, and the corresponding psbA, psbD and cab mRNAs also decreased more dramatically in WT; CO2 assimilation, stomatal conductance, maximum efficiency of PSII photochemistry (Fv/Fm), efficiency of excitation energy capture by open PSII reaction centres (Fv’/Fm’), quantum yield of PSII electron transport (φPSII) and DCIP photoreduction in NYB were less sensitive to water stress than in WT, although the non-photochemical quenching coefficient (qN) and the photochemical quenching coefficient (qP) were almost the same in NYB and WT. Effective chlorophyll utilization and improved PSII protein formation in the mutant may be the reason for the enhanced stress resistance. Other possible mechanisms are also discussed.

Phosphate Deficiency Increases the Rate Constant of Thermal Dissipation of Excitation Energy by Photosystem II in Intact Leaves of Sunflower and Maize.

1995 ◽  
Vol 22 (3) ◽  
pp. 417 ◽  
Author(s):  
J Jacob
Keyword(s):  
Excitation Energy ◽  
Rate Constant ◽  
Thermal Dissipation ◽  
Phosphate Deficiency ◽  
Photon Flux ◽  
Photochemical Quenching ◽  
Variable Fluorescence ◽  
Psii Photochemistry ◽  
Non Photochemical Quenching

Sunflower (Helianthus annuus L.) and maize (Zea mays L.) plants were grown in controlled environment chambers either with adequate supply or no external supply of inorganic phosphate. On the third fully-expanded leaves, chlorophyll fluorescence from photosystem II (PSII) was measured using a modulated fluorescence measuring system at various photon flux densities at room temperature. Phosphate deficiency resulted in an increase in the coefficient of non-photochemical quenching and a decrease in the coefficient of photochemical quenching of variable fluorescence. The efficiency of excitation energy capture by open PSII reaction centres and quantum yield of PSII photochemistry were decreased with phosphate deficiency. There was a significant effect of phosphate deficiency on in vivo PSII photochemistry which was independent of changes in thylakoid membrane energisation induced by the actinic light. An increase in the non-photochemical quenching of variable fluorescence with phosphate deficiency was due to an increased rate constant of thermal dissipation of excitation energy by PSII. Analyses of fluorescence signals suggest that phosphate deficiency decreased the rate constant of PSII photochemistry as well as the probability of excitation energy transfer from PSII antenna to PSII reaction centre. These effects were more apparent at low photon flux densities than at high photon flux densities. Regulation of energy transduction in the thylakoid and in vivo PSII activity in response to the physical environment of the plant are important aspects of environmental regulation of photosynthesis.

scholarly journals Assessment of Heat-tolerance Potential in QTL Introgressed Lines of Hybrid Rice Restorer, KMR-3R through PS-II Efficiency

2021 ◽  
pp. 258-266
Author(s):  
V. Jaldhani ◽  
D. Sanjeeva Rao ◽  
P. Beulah ◽  
B. Srikanth ◽  
P. R. Rao ◽  
...  
Keyword(s):  
High Temperature ◽  
Quantum Yield ◽  
Temperature Stress ◽  
Heat Tolerance ◽  
Photosynthetic Apparatus ◽  
High Temperature Stress ◽  
Maximum Efficiency ◽  
Photochemical Quenching ◽  
Psii Photochemistry ◽  
Heat Tolerant

Aims: To assess heat-induced PSII damage and efficiency in eight promising backcross introgression lines (BC2F6) of KMR-3R/N22 possessing qHTSF1.1 and qHTSF4.1. Study Design:  Randomized Complete Block Design (RCBD) with three replications. Place and Duration of Study: ICAR-Indian Institute of Rice Research, Hyderabad India during wet/rainy (Kharif) season 2018. Methodology: Eight ILs (BC2F6) and parents were evaluated for heat tolerance. The high- temperature stress was imposed by enclosing the crop with a poly cover tent (Polyhouse) just before the anthesis stage. The fluorescence parameters viz., maximum efficiency of PSII photochemistry (Fv/Fm), Electron transport rate (ETR), effective PSII quantum yield (ΦPSII), coefficient of photochemical quenching (qP) and coefficient of non-photochemical quenching (qN) were measured under ambient and high-temperature stress. Results: The heat-tolerance potential of ILs was assessed in terms of PSII activity. The results indicated that significant differences were observed between treatments (T), genotypes (G) and the interaction between T × G.  The physiological basis of introgressed QTLs controls the spikelet fertility by maintaining the productive and adaptive strategies in heat-tolerant QTL introgressed lines with stable photosynthetic apparatus (PSII) under high-temperature stress. Conclusion: The Fv/Fm ratio denotes the maximum quantum yield of PSII. The heat-tolerant QTL introgressed lines exhibited stable photosynthetic apparatus (PSII) and noted better performance under high-temperature stress. They may be used as donors for fluorescence traits in breeding rice for high-temperature tolerance.

scholarly journals Changes in Photo-Protective Energy Dissipation of Photosystem II in Response to Beneficial Bacteria Consortium in Durum Wheat under Drought and Salinity Stresses

2020 ◽  
Vol 10 (15) ◽  
pp. 5031 ◽  
Author(s):  
Mohammad Yaghoubi Khanghahi ◽  
Sabrina Strafella ◽  
Carmine Crecchio
Keyword(s):  
Chlorophyll Fluorescence ◽  
Energy Dissipation ◽  
Quantum Yield ◽  
Photosystem Ii ◽  
Excitation Energy ◽  
Durum Wheat ◽  
Photochemical Quenching ◽  
Plant Growth Promoting Bacteria ◽  
Psii Photochemistry ◽  
Non Photochemical Quenching

The present research aimed at evaluating the harmless dissipation of excess excitation energy by durum wheat (Triticum durum Desf.) leaves in response to the application of a bacterial consortium consisting of four plant growth-promoting bacteria (PGPB). Three pot experiments were carried out under non-stress, drought (at 40% field capacity), and salinity (150 mM NaCl) conditions. The results showed that drought and salinity affected photo-protective energy dissipation of photosystem II (PSII) increasing the rate of non-photochemical chlorophyll fluorescence quenching (NPQ (non-photochemical quenching) and qCN (complete non-photochemical quenching)), as well as decreasing the total quenching of chlorophyll fluorescence (qTQ), total quenching of variable chlorophyll fluorescence (qTV) and the ratio of the quantum yield of actual PSII photochemistry, in light-adapted state to the quantum yield of the constitutive non-regulatory NPQ (PQ rate). Our results also indicated that the PGPB inoculants can mitigate the adverse impacts of stresses on leaves, especially the saline one, in comparison with the non-fertilized (control) treatment, by increasing the fraction of light absorbed by the PSII antenna, PQ ratio, qTQ, and qTV. In the light of findings, our beneficial bacterial strains showed the potential in reducing reliance on traditional chemical fertilizers, in particular in saline soil, by improving the grain yield and regulating the amount of excitation energy.

scholarly journals Chlorophyll α Fluorescence Parameters as an Indicator to Identify Drought Susceptibility in Common Bush Bean

Agronomy ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 526 ◽  
Author(s):  
Alefsi David Sánchez-Reinoso ◽  
Gustavo Adolfo Ligarreto-Moreno ◽  
Hermann Restrepo-Díaz
Keyword(s):  
Water Deficit ◽  
Block Design ◽  
Reproductive Stage ◽  
Vegetative Stage ◽  
Initial Slope ◽  
Maximum Efficiency ◽  
Photochemical Quenching ◽  
Psii Photochemistry ◽  
Drought Conditions ◽  
Bush Bean

The common bean is susceptible to drought conditions and the evaluation of plant responses to low water availability can be difficult. The quantification of chlorophyll fluorescence as a sensitive trait to environmental stresses is an important alternative in the characterization of drought-susceptible genotypes. The objective of this study was to evaluate mainly the use of chlorophyll α fluorescence (maximum efficiency of PSII (Fv/Fm), photochemical quenching (qP), non-photochemical quenching (NPQ)) and rapid light-response curves (RLCs) (initial slope of the curve (α), minimum saturation irradiance (Ik) and maximum relative electron transport rate (ETRmax)) parameters as tools for the identification of susceptible or tolerant bush bean cultivars to water deficit stress conditions in two different phenological stages. Using a randomized block design in a factorial arrangement, five bush bean cultivars (Cerinza, Bachue, NUA35, Bacata and Bianca) were evaluated under water deficit conditions by the suspension of irrigation for 15 days from 40 to 55 Days after Emergence (DAE) (vegetative stage) or 50 to 65 DAE (reproductive stage). The results showed that Fv/Fm and NPQ recorded the highest variation due to water deficit conditions, especially in the vegetative stage. The greatest reductions in Fv/Fm (0.67) and NPQ (0.71) were evidenced in cultivar NUA35 compared to its control plants (0.78 and 1.07, respectively). The parameters obtained from RLCs showed that cultivar Bacata registered the lowest α (0.17) and Ik (838.19 μmol∙m−2∙s−1) values compared to its control plants (α 0.23; Ik 769.99 μmol∙m−2∙s−1). Differences were only obtained in ETRmax in the reproductive stage (50–65 DAE) in which cultivar NUA35 reached values of 158.5 in stressed plants compared to control plants (251.22). In conclusion, the parameters derived from RLCs such as α and Ik can be used as tools to identify drought susceptibility in the vegetative stage, whereas ETRmax can be used in the reproductive stage. In addition, PSII photochemistry (Fv/Fm and NPQ) can also help to understand the agronomic responses of common bush bean cultivars to drought conditions.

scholarly journals Photosynthetic responses of young cashew plants to varying environmental conditions

2005 ◽  
Vol 40 (8) ◽  
pp. 735-744 ◽  
Author(s):  
Rogéria Pereira de Souza ◽  
Rafael Vasconcelos Ribeiro ◽  
Eduardo Caruso Machado ◽  
Ricardo Ferraz de Oliveira ◽  
Joaquim Albenísio Gomes da Silveira
Keyword(s):  
Gas Exchange ◽  
Intercellular Co2 Concentration ◽  
Co2 Concentration ◽  
Co2 Assimilation ◽  
Photon Flux ◽  
Photochemical Quenching ◽  
Photon Flux Density ◽  
Effective Quantum Yield ◽  
Water Losses ◽  
Controlled Conditions

The aim of this study was to characterize gas exchange responses of young cashew plants to varying photosynthetic photon flux density (PPFD), temperature, vapor-pressure deficit (VPD), and intercellular CO2 concentration (Ci), under controlled conditions. Daily courses of gas exchange and chlorophyll a fluorescence parameters were measured under natural conditions. Maximum CO2 assimilation rates, under optimal controlled conditions, were about 13 mmol m-2 s-1 , with light saturation around 1,000 mmol m-2 s-1. Leaf temperatures between 25ºC and 35ºC were optimal for photosynthesis. Stomata showed sensitivity to CO2, and a closing response with increasing Ci. Increasing VPD had a small effect on CO2 assimilation rates, with a small decrease above 2.5 kPa. Stomata, however, were strongly affected by VPD, exhibiting gradual closure above 1.5 kPa. The reduced stomatal conductances at high VPD were efficient in restricting water losses by transpiration, demonstrating the species adaptability to dry environments. Under natural irradiance, CO2 assimilation rates were saturated in early morning, following thereafter the PPFD changes. Transient Fv/Fm decreases were registered around 11h, indicating the occurrence of photoinhibition. Decreases of excitation capture efficiency, decreases of effective quantum yield of photosystem II, and increases in non-photochemical quenching were consistent with the occurrence of photoprotection under excessive irradiance levels.

scholarly journals Hormetic Responses of Photosystem II in Tomato to Botrytis cinerea

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 521
Author(s):  
Maria-Lavrentia Stamelou ◽  
Ilektra Sperdouli ◽  
Ioanna Pyrri ◽  
Ioannis-Dimosthenis S. Adamakis ◽  
Michael Moustakas
Keyword(s):  
Photosystem Ii ◽  
Botrytis Cinerea ◽  
Defense Response ◽  
Gray Mold ◽  
Spore Suspension ◽  
Photochemical Quenching ◽  
Adjacent Area ◽  
Solanum Lycopersicum L ◽  
Psii Photochemistry ◽  
Non Photochemical Quenching

Botrytis cinerea, a fungal pathogen that causes gray mold, is damaging more than 200 plant species, and especially tomato. Photosystem II (PSII) responses in tomato (Solanum lycopersicum L.) leaves to Botrytis cinerea spore suspension application were evaluated by chlorophyll fluorescence imaging analysis. Hydrogen peroxide (H2O2) that was detected 30 min after Botrytis application with an increasing trend up to 240 min, is possibly convening tolerance against B. cinerea at short-time exposure, but when increasing at relative longer exposure, is becoming a damaging molecule. In accordance, an enhanced photosystem II (PSII) functionality was observed 30 min after application of B. cinerea, with a higher fraction of absorbed light energy to be directed to photochemistry (ΦPSΙΙ). The concomitant increase in the photoprotective mechanism of non-photochemical quenching of photosynthesis (NPQ) resulted in a significant decrease in the dissipated non-regulated energy (ΦNO), indicating a possible decreased singlet oxygen (1O2) formation, thus specifying a modified reactive oxygen species (ROS) homeostasis. Therefore, 30 min after application of Botrytis spore suspension, before any visual symptoms appeared, defense response mechanisms were triggered, with PSII photochemistry to be adjusted by NPQ in a such way that PSII functionality to be enhanced, but being fully inhibited at the application spot and the adjacent area, after longer exposure (240 min). Hence, the response of tomato PSII to B. cinerea, indicates a hormetic temporal response in terms of “stress defense response” and “toxicity”, expanding the features of hormesis to biotic factors also. The enhanced PSII functionality 30 min after Botrytis application can possible be related with the need of an increased sugar production that is associated with a stronger plant defense potential through the induction of defense genes.

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