The involvement of the mitochondrial peroxiredoxin PRXIIF in defining physiological differences between orthodox and recalcitrant seeds of two Acer species

2013 ◽  
Vol 40 (10) ◽  
pp. 1005 ◽  
Author(s):  
Ewelina Ratajczak ◽  
Elke Ströher ◽  
Marie-Luise Oelze ◽  
Ewa M. Kalemba ◽  
Stanisława Pukacka ◽  
...  
Keyword(s):  
Transcript Level ◽  
Climatic Conditions ◽  
Amino Acid Sequences ◽  
Initial Water Content ◽  
2D Electrophoresis ◽  
Acer Pseudoplatanus ◽  
Recalcitrant Seeds ◽  
Initial Water ◽  
Norway Maple ◽  
Physiological Differences

Norway maple (Acer platanoides L., orthodox) and sycamore (Acer pseudoplatanus L., recalcitrant) belong to the same genus and grow under similar climatic conditions, but their seeds differ in their tolerance to desiccation. The initial water content (WC) of the seeds used in this study was 50%, and they were dried to 40, 20 and 7%. The mitochondrial peroxiredoxin IIF (PRXIIF) was identified in seeds of both species by immunoblotting. Semiquantitative RT–PCR analyses indicated that the transcript level of PRXIIF in both types of seeds increased during different stages of desiccation and was higher in seeds of Norway maple than in sycamore. General proteome analyses showed important differences between orthodox and recalcitrant seeds. In sycamore seeds that had been desiccated to a 7% WC, the number of protein spots and the levels of those spots were lower than in desiccation-tolerant Norway maple seeds. Post-translational modifications of PRXIIF in seeds at a 50% WC were detected via 2D electrophoresis and subsequent western blot analysis. The detected shift in the pI values (± 0.3) in A. pseudoplatanus was possibly caused by phosphorylation because several potential phosphorylation sites were predicted in silico for that protein. The gene and amino acid sequences were obtained and aligned with known sequences of other plant PRXIIF genes and proteins. High values of sequence identity were noted between the PRXIIF protein sequences of Acer species, Populus trichocarpa Torr. & A. Gray and Arabidopsis thaliana (L.) Heynh. The involvement of PRXIIF in defining the physiological differences between desiccation-tolerant and desiccation-sensitive Acer seeds is discussed in the context of its role in mitochondrial redox homeostasis.

scholarly journals Changes in Proline Levels during Seed Development of Orthodox and Recalcitrant Seeds of Genus Acer in a Climate Change Scenario

Forests ◽  
2020 ◽  
Vol 11 (12) ◽  
pp. 1362
Author(s):  
Joanna Kijowska-Oberc ◽  
Aleksandra M. Staszak ◽  
Mikołaj K. Wawrzyniak ◽  
Ewelina Ratajczak
Keyword(s):  
Climate Change ◽  
Seed Development ◽  
Desiccation Tolerance ◽  
Seed Viability ◽  
Climatic Conditions ◽  
Proline Content ◽  
Change Scenario ◽  
Acer Pseudoplatanus ◽  
Recalcitrant Seeds ◽  
Seed Desiccation

In the present study, we examined the utility of proline usage as a biochemical indicator of metabolic changes caused by climate change (mean temperature and precipitation) during seed development of two Acer species differing in desiccation tolerance: Norway maple (Acer platanoides L.—desiccation tolerant—orthodox) and sycamore (Acer pseudoplatanus L.—desiccation sensitive—recalcitrant). In plants, proline is an element of the antioxidant system, which has a role in response to water loss and high temperatures. Our study considered whether proline could be treated as an indicator of tree seed viability, crucial for genetic resources conservation. Proline content was measured biweekly in developing seeds (between 11 and 23 weeks after flowering) collected in consecutive years (2017, 2018, and 2019). We showed that proline concentrations in recalcitrant seeds were positively correlated with mean two-week temperature. In contrast, in orthodox seeds no such relationship was found. Proline content proved to be sensitive to thermal-moisture conditions changes, which makes it a promising biochemical marker of seed desiccation tolerance in different climatic conditions.

scholarly journals Proposal Solar Drying With Heat Storage Applied to Medicinal Plants

2019 ◽  
Vol 11 (16) ◽  
pp. 80
Author(s):  
Valentin Silvera Diaz ◽  
Eduardo Gonçalves Reimbrecht ◽  
Tales Jahn ◽  
Oswaldo Hideo Ando Junior
Keyword(s):  
Climatic Conditions ◽  
Initial Water Content ◽  
Air Velocity ◽  
Total Energy Consumption ◽  
Initial Water ◽  
Drying Time ◽  
System A ◽  
Solar Fraction ◽  
The City ◽  
And Storage

In this work, a proposal of an indirect solar dryer with a vacuum solar collector and storage of water-sensitive heater is presented. A mathematical model is presented as the first approximation to evaluate the performance. The test climatic conditions are based on the city of Cascavel (Paraná, Brazil) and the resolution of the model was aided by the EES program. In order to analyze the performance of the system, a model load was created based on the references found in the literature, sample of 80 kg with initial water content of 75% w.b and final of 10% w.b, for drying time of 4 h and 3 h with air velocity of 0.7 m/s and 1.1 m/s respectively. It was possible to simulate the variation of the temperature of the reservoir during the day, as well as the response of the heat exchanger to the variation of temperature of entrance of the fluids and the climatic influence, radiation and ambient temperature in the participation of the solar energy in the total energy consumption of the drying. The simulations suggest good results with solar fraction between 20 and 47 %, meanwhile in the literature the values reported are between 10 and 25%.

Influence of initial water content and strain rate on remolded yield stress in marine clay

2021 ◽  
pp. 1-8
Author(s):  
Xiaobing Li ◽  
Jianpeng Chen ◽  
Xiuqing Hu ◽  
Hongtao Fu ◽  
Jun Wang ◽  
...  
Keyword(s):  
Strain Rate ◽  
Water Content ◽  
Yield Stress ◽  
Initial Water Content ◽  
Marine Clay ◽  
Initial Water

scholarly journals Application of the Calorimetric Methods to the Characteristics of Seeds from Olives

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 90
Author(s):  
Andrzej Bryś ◽  
Joanna Bryś ◽  
Marko Obranović ◽  
Dubravka Škevin ◽  
Szymon Głowacki ◽  
...  
Keyword(s):  
Olive Oil ◽  
Water Content ◽  
Cooling System ◽  
Waste Product ◽  
Normal Pressure ◽  
Ash Content ◽  
Initial Water Content ◽  
Scanning Calorimetry ◽  
Initial Water

The olive oil industry represents an important productive sector in the Mediterranean basin countries. Olive stone is an essential by-product generated in the olive oil extraction industries and it represents roughly 10% by weight of the olive fruit. The seeds of pickled olives are also a significant waste product. In the present study, we have investigated the possibility of the use of differential scanning calorimetry for the thermal characterization of seeds from green and black pickled olives from Croatia. The differential scanning calorimeter (DSC) with a normal pressure cell equipped with a cooling system was used to determine the thermal properties of seeds from olives. The following analyses were also performed: the determination of calorific values in a pressure bomb calorimeter, the determination of initial water content, the determination of changes of water content during drying at the temperatures of 30 °C, 50 °C and 80 °C, the determination of a percentage content of seeds mass to the mass of the whole olives, and the determination of ash content. Seeds from olives are characterized by very good parameters as a biomass. The analyzed olive seeds were characterized by low water content, low ash content, and a relatively high caloric value.

scholarly journals RESEARCH ON MODEL FITTING AND STRENGTH CHARACTERISTICS OF CRITICAL STATE FOR EXPANSIVE SOIL

2013 ◽  
Vol 19 (1) ◽  
pp. 9-15 ◽  
Author(s):  
Zhiqing Li ◽  
Chuan Tang ◽  
Ruilin Hu ◽  
Yingxin Zhou
Keyword(s):  
Critical State ◽  
Model Fitting ◽  
Expansive Soil ◽  
Dry Density ◽  
Initial Water Content ◽  
Test Results ◽  
Stress Strain ◽  
Initial Water ◽  
Curve Model ◽  
Shearing Strength

According to Mengzi expansive soil, consolidated drained tests and undrained tests are carried on under saturated and remoulded conditions. The stress-strain characteristics of saturated soil are researched systematically under different confining pressure, initial dry density, initial water content, shearing rate and drainage condition. The inherent unity of diversity of shearing strength for the same samples measured by different experimental methods is indicated according to the normalization of critical state test results. And the failure lines in p ‘- q - ν space of remoulded saturated expansive soil under consolidated drained and undrained conditions are attained. The hyperbolic curve model can fit well the weak hardening stress-strain curves and the exponential curve model can fit the weak softening stress-strain curves. The test results can provide technical parameters and theoretical help for shearing strength variation of slope during rainfall and strength state of soil structure in normal water level.

THREE-DIMENSIONAL SEEPAGE ANALYSIS OF A COAL REFUSE PILE RECLAMATION

2020 ◽  
Vol 9 (4) ◽  
pp. 44-66
Author(s):  
Iuri Lira Santos ◽  
Keyword(s):  
Mine Drainage ◽  
Characteristic Curve ◽  
Water Volume ◽  
Low Permeability ◽  
Initial Water Content ◽  
Initial Water ◽  
Seepage Analysis ◽  
Cover System ◽  
Coal Refuse ◽  
Refuse Pile

Abstract. A coal refuse pile located in Greenbrier County, West Virginia was studied to restrict generation of acid mine drainage through the use of a cap and cover system. This paper presents results of a finite element method seepage analysis on a proposed reclamation design. The proposed reclamation incorporates a cap and cover system with a 0.3-m thick surface vegetation cap layer over a 0.6-m thick low permeability layer. The low permeability layer is directly above the coal refuse. Unsaturated soil mechanics was utilized, adopting the Fredlund and Xing equation for soil-water characteristic curve (SWCC) estimation. SWCC fitting parameters were calculated using the Zapata and the Hernandez estimation techniques. Different precipitation events were used to evaluate seepage throughout the reclamation area and assess the effectiveness of the cap and cover system. A steep area (>4H:1V) and a flat area were considered. The water balance analysis showed a 50% to 88% reduction in water volume at the coal refuse layer and a reduction in the time for the refuse to return to initial water content due to the cap and cover system implementation. Moisture detainment was observed in the growth layer and is important for supporting vegetation persistence.

scholarly journals Effects of Initial Water Content on Microstructure and Mechanical Properties of Lean Clay Soil Stabilized by Compound Calcium-Based Stabilizer

Materials ◽  
2018 ◽  
Vol 11 (10) ◽  
pp. 1933 ◽  
Author(s):  
Chenglong Yin ◽  
Wei Zhang ◽  
Xunli Jiang ◽  
Zhiyi Huang
Keyword(s):  
Water Content ◽  
Soil Stabilization ◽  
Clay Soil ◽  
Soil Aggregates ◽  
Optical Microscope ◽  
Initial Water Content ◽  
Reaction Products ◽  
Unconfined Compression Test ◽  
Initial Water ◽  
X Ray

Initial water content significantly affects the efficiency of soil stabilization. In this study, the effects of initial water content on the compressibility, strength, microstructure, and composition of a lean clay soil stabilized by compound calcium-based stabilizer were investigated by static compaction test, unconfined compression test, optical microscope observations, environment scanning electron microscopy, energy dispersive X-ray spectroscopy, and X-ray diffraction. The results indicate that as the initial water content increases in the range studied, both the compaction energy and the maximum compaction force decrease linearly and there are less soil aggregates or agglomerations, and a smaller proportion of large pores in the compacted mixture structure. In addition, for specimens cured with or without external water supply and under different compaction degrees, the variation law of the unconfined compressive strength with initial water content is different and the highest strength value is obtained at various initial water contents. With the increase of initial water content, the percentage of the oxygen element tends to increase in the reaction products of the calcium-based stabilizer, whereas the primary mineral composition of the soil-stabilizer mixture did not change notably.

Effects of differential drying rates on viability retention of recalcitrant seeds of Ekebergia capensis

1998 ◽  
Vol 8 (4) ◽  
pp. 463-471 ◽  
Author(s):  
N. W. Pammenter ◽  
Valerie Greggains ◽  
J. I. Kioko ◽  
J. Wesley-Smith ◽  
Patricia Berjak ◽  
...  
Keyword(s):  
Water Content ◽  
Exponential Function ◽  
Time Course ◽  
Drying Rate ◽  
Recalcitrant Seeds ◽  
Tissue Water ◽  
Initial Water ◽  
Viability Loss ◽  
Drying Rates ◽  
Water Contents

AbstractThe drying rate of whole seeds of Ekebergia capensis (Meliaceae) was shown to influence the response to desiccation, with rapidly dried seeds surviving to lower water contents. Short-term rapid drying (to water contents higher than those leading to viability loss) actually increased the rate of germination. The form of the time course of decline of axis water content varied with drying rate; slow drying could be described by an exponential function, whereas with rapid drying initial water loss was faster than predicted by an exponential function. These observations suggest that slow drying brought about homogeneous dehydration and that the rapid drying was uneven across the tissue. This raised the possibility that the different responses to dehydration were a function of different distributions of water in the axis tissue under the two drying regimes. However, ultrastructural observations indicated that different deleterious processes may be occurring under the different drying treatments. It was tentatively concluded that a major cause of viability loss in slowly dried material was likely to be a consequence of aqueous-based processes leading to considerable membrane degradation. Uneven distribution of tissue water could not be rejected as a contributory cause of the survival of rapidly dried seeds to low bulk water contents. The differential response to dehydration at different drying rates implies that it is not possible to determine a ‘critical water content’ for viability loss by recalcitrant seeds.

scholarly journals Solidification of High Organic Matter Content Sludge by Cement, Lime and Metakaolin

2018 ◽  
Author(s):  
Ke Rui ◽  
Wang Hongxing ◽  
Tan Yunzhi ◽  
Wang Lehua
Keyword(s):  
Organic Matter ◽  
Water Content ◽  
Organic Matter Content ◽  
Microscopic Analysis ◽  
Chemical Components ◽  
Orthogonal Experimental Design ◽  
Initial Water Content ◽  
Initial Water ◽  
Lime Content ◽  
Sheet Structure

Based on orthogonal experimental design, the key solidification controlling technology of Solidified/Stabilized (S/S) sludge with high total organic content (TOC) by cement, lime and metakaolin was explored by macroscopic tests, chemical components measurements and microscopic analysis. The macroscopic tests show that, the permeability coefficient is mainly affected by initial water content and lime content, and the unconfined compression strength is mainly affected by cement content and lime content. The chemical components measurements show that, the solidification effect of S/S sludge with high TOC is controlled by organic matter consumption, and organic matter consumption is determined by the alkaline environment from the cement and lime hydration reactions, which is mainly affect by the initial water content and lime-metakaolin content ratio. The microscopic analysis results show that, lime consumes parts of organic matter while excess lime produces weak Ca(OH)2 crystal fluffy sheet structure, matakaolin produces pozzolanic reactions with cement and lime instead of soil particles, and consumes the weak Ca(OH)2 crystal fluffy sheet structure produced by superfluous lime. The research has confirmed key controlling points of S/S sludge in case of high TOC, which will provide theoretical guidance and technical support for S/S sludge promotion with high TOC.

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