Virus indexing in the New South Wales citrus improvement scheme

1992 ◽  
Vol 32 (4) ◽  
pp. 493 ◽  
Author(s):  
P Broadbent ◽  
CM Dephoff

A virus indexing program was initiated in the 1950s to test local citrus clones with commercial potential, for the presence of citrus exocortis viroid and psorosis. As indexing techniques improved, clones have also been tested for the presence of citrus cachexia viroid and other viroids, citrus infectious variegation virus, citrus tatter leaf virus, and strain severity of citrus tristeza virus. Of 123 candidate clones tested, only 2 were infected with psorosis virus. Crinkly leaf (infectious variegation virus) was widespread in lemons but has been eliminated by nucellar embryony. The widespread incidence of citrus exocortis and other lower molecular weight viroids has been reduced by screening mother trees on Poncirus trifoliata stocks, biological indexing, and, more recently, by sequential polyacrylamide gel electrophoresis of RNA extracts. Cachexia is rarely found in Australian citrus clones.

Author(s):  

Abstract A new distribution map is provided for Citrus exocortis viroid. Viroid: Pospiviroidae: Pospiviroid. Hosts: Citrus spp. and Trifoliate orange (Poncirus trifoliata). Information is given on the geographical distribution in Europe (Cyprus, France (Corsica), Italy (mainland Italy, Sardinia, Sicily), Portugal, Russia (European Russia), Serbia and Montenegro, Spain), Asia (China (Fujian, Hubei, Hunan, Sichuan, Zhejiang), India (Delhi, Maharashtra, Pubjab), Indonesia, Iran, Iraq, Israel, Japan (Honshu), Jordan, Korea Republic, Lebanon, Malaysia, Oman, Pakistan, Philippines, Saudi Arabia, Syria, Taiwan, Thailand, Turkey, United Arab Emirates, Vietnam, Yemen), Africa (Algeria, Cameroon, Cote d'Ivoire, Egypt, Ethiopia, Ghana, Libya, Madagascar, Mauritius, Morocco, Mozambique, Nigeria, Reunion, Sierra Leone, Somalia, South Africa, Sudan, Tunisia), North America (Mexico, USA (Arizona, California, Florida, Louisiana, Texas)), Central America and Caribbean (Cuba, Guadeloupe, Jamaica, Trinidad and Tobago), South America (Argentina, Bolivia, Brazil (Sao Paulo), Chile, Colombia, Peru, Suriname, Uruguay, Venezuela), Oceania (Australia (New South Wales, Northern Territory, Queensland, Victoria), Cook Islands, Fiji, French Polynesia, New Zealand, Papua New Guinea).


1989 ◽  
Vol 29 (2) ◽  
pp. 273 ◽  
Author(s):  
GAC Beattie ◽  
EA Roberts ◽  
LE Rippon ◽  
CL Vanhoff

The phytotoxicity of petroleum oil sprays to Vakncia orange (Citrus sinensis (L.) Osbeck) on Poncirus trifoliata (L.) Raf. rootstocks was investigated at Kulnura and at Griffith, New South Wales. Four narrow-range petroleum oils, designated as 206, 212, 214 and 238 oils, were applied as high volume sprays in 3 consecutive years. Annual sprays were applied during the spring, summer and autumn months of November, February and April. Some treatments were sprayed once each year, others twice. The single spray per year treatments involved concentrations of 1.2 or 2% of each oil in February or 2% of the 212, 214 and 238 oils in November or in April. A total concentration of 3.2% oil per year of the 2 12,2 14 and 238 oils was applied in February (2%) and in November of the previous year (1.2%) or in April (1.2%) to the double spray treatments. The effect of the sprays on yield variables, percentage juice, �Brix (w/w sucrose), anhydrous citric acid, rind colour and oil content was studied. Rind colour was not affected by any treatment at either location. Significant (P< 0.05), though inconsistent, effects on other characteristics of fruit quality were found. Increase in 50% distillation temperature and rate of application of oils tended to decrease �Brix. The oils did not significantly affect yield at Kulnura. At Griffith, 2% sprays of 238 oil significantly reduced yield by reducing the number of fruit. At both locations, trees sprayed with 2% oil in April tended to have lower yields than those sprayed in November or February at the same rate. The effect of sprays with total concentrations of 3.2% was variable. The 238 oil was the only oil considered to be potentially phytotoxic. This type of oil should not be used at concentrations > 2% from March to November or in consecutive years at any time. They can be used from late spring to early autumn in consecutive years at 1.2%. Variation in the phytotoxicity of oils between locations in south-eastern Australia appears to be related to variation in night temperatures in summer and early autumn which affect flower initiation.


Genetics ◽  
1998 ◽  
Vol 150 (2) ◽  
pp. 883-890
Author(s):  
D Q Fang ◽  
C T Federici ◽  
M L Roose

Abstract Resistance to citrus tristeza virus (CTV) was evaluated in 554 progeny of 10 populations derived from Poncirus trifoliata. A dominant gene (Ctv) controlled CTV resistance in P. trifoliata. Twenty-one dominant PCR-based DNA markers were identified as linked to Ctv by bulked segregant analysis. Of the 11 closest markers to Ctv, only 2 segregated in all populations. Ten of these markers were cloned and sequenced, and codominant RFLP markers were developed. Seven RFLP markers were then evaluated in 10 populations. Marker orders were consistent in all linkage maps based on data of single populations or on combined data of populations with similar segregation patterns. In a consensus map, the six closest marker loci spanned 5.3 cM of the Ctv region. Z16 cosegregated with Ctv. C19 and AD08 flanked Ctv at distances of 0.5 and 0.8 cM, respectively. These 3 markers were present as single copies in the Poncirus genome, and could be used directly for bacterial artificial chromosome library screening to initiate a walk toward Ctv. BLAST searches of the GenBank database revealed high sequence similarities between 2 markers and known plant disease resistance genes, indicating that a resistance gene cluster exists in the Ctv region in P. trifoliata.


1992 ◽  
Vol 32 (2) ◽  
pp. 205
Author(s):  
RA Sarooshi ◽  
P Broadbent

The yield, growth, fruit quality, and graft compatibility of 2 lemon cultivars Eureka and Lisbon [Citrus limon (L.) Bum. f.], budded onto several new rootstocks, were studied in replant ground.Promising rootstocks for Eureka lemon were 2 new hybrids bred at Gosford, New South Wales, 3798 (Scarlet mandarin x Poncirus trifoliata) and 4017 (Smooth Seville x P. trifoliata), and also Benton citrange and Nelspruit hybrid 639. Trees on the sour orange group of rootstocks including Xingshan, Dai Dai and Baggan yielded 44-63% less than trees on rough lemon rootstocks; but their fruit quality, as evidenced by juice per cent, OBrix, and citric acid (kg/t), was better. The performance of Lisbon lemon was tested on 10 selections of F'. trifoliata, and although trees on Flying Dragon were smaller and yielded about 50% less than on Swingle, Christiansen, Large Flower, and selection 22, results were not significantly (P<0.05) different.


Author(s):  

Abstract A new distribution map is provided for Citrus exocortis viroid. Pospiviroidae: Pospiviroid. Hosts: Citrus spp. and trifoliate orange (Poncirus trifoliata). Information is given on the geographical distribution in Europe (Austria, Belgium, Cyprus, Czech Republic, France, Germany, Greece, Italy, Sardinia, Sicily, Montenegro, Netherlands, Portugal, Russia, Slovenia and Spain), Asia (China, Chongqing, Fujian, Henan, Hubei, Hunan, Liaoning, Shandong, Sichuan, Zhejiang, India, New Delhi, Maharashtra, Indian Punjab, Indonesia, Iran, Iraq, Israel, Japan, Honshu, Jordan, Korea Republic, Lebanon, Malaysia, Oman, Pakistan, Philippines, Saudi Arabia, Syria, Taiwan, Thailand, Turkey, United Arab Emirates, Vietnam and Yemen), Africa (Algeria, Cameroon, Cote d'Ivoire, Egypt, Ethiopia, Ghana, Libya, Madagascar, Mauritius, Morocco, Mozambique, Nigeria, Reunion, Sierra Leone, Somalia, South Africa, Sudan and Tunisia), North America (Canada, New Brunswick, Mexico, USA, Arizona, California, Florida, Louisiana and Texas), Central America and Caribbean (Cuba, Guadeloupe, Jamaica and Trinidad and Tobago), South America (Argentina, Bolivia, Brazil, Sao Paulo, Chile, Colombia, Peru, Suriname, Uruguay and Venezuela) and Oceania (Australia, New South Wales, Northern Territory, Queensland, Victoria, Cook Islands, Fiji, French Polynesia, New Zealand, Papua New Guinea and Samoa).


2017 ◽  
Vol 46 (4) ◽  
pp. 351-355 ◽  
Author(s):  
Yan Zhou ◽  
Yinjie Liu ◽  
Kehong Liu ◽  
Fangyun Yang ◽  
Changyong Zhou

1997 ◽  
Vol 95 (4) ◽  
pp. 691-695 ◽  
Author(s):  
P. F. Mestre ◽  
M. J. Asíns ◽  
E. A. Carbonell ◽  
L. Navarro

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