An evaluation of ammonium sulphate as a potential NPN source for ruminants

1976 ◽  
Vol 16 (83) ◽  
pp. 838 ◽  
Author(s):  
CA Graham ◽  
RB Warner ◽  
SL Jenkins

Experiments were undertaken to examine the comparative toxicity to sheep of ammonium sulphate and urea given as single doses and the value of ammonium sulphate fed alone or in combination with urea as a supplement to low nitrogen roughages. Ammonium sulphate dosing produced significantly lower mean blood ammonia levels than urea dosing. Fifteen grams of nitrogen as ammonium sulphate were tolerated as a single dose but the equivalent level of urea was toxic. lnappetance and ruminal stasis was noted when sheep fed a low nitrogen roughage (0.5 per cent nitrogen) received ammonium sulphate supplement as the sole non-protein nitrogen source. Ruminal stasis and inappetance may have been due to sulphide toxicity. Ammonium sulphate when used at lower levels in varying combinations with urea was well utilized as a nitrogen and sulphur source even when supplying 50 per cent of the supplemented nitrogen.

1969 ◽  
Vol 49 (2) ◽  
pp. 135-141 ◽  
Author(s):  
L. P. Milligan ◽  
A. R. Robblee ◽  
J. C. Wood ◽  
W. C. Kay ◽  
S. K. Chakrabartty

The preparation of a polymer of urea and furfural containing 23.2% nitrogen is described. This product was converted by rumen microorganisms in vitro to ammonia at a rate approximately one-seventh that of conversion of urea to ammonia. Use of the polymer as a dietary supplement in a feeding trial with lambs improved nitrogen retention over that of unsupplemented controls by 3.45 g of nitrogen retained per day, while an isonitrogenous quantity of supplemental urea improved nitrogen retention by 0.51 g of nitrogen retained per day. The blood urea pattern, throughout the day, of lambs adapted to control, urea-supplemented and urea–furfural polymer-supplemented rations indicated a slow, prolonged production of ammonia from the latter supplement and very rapid, short-term degradation of urea in vivo.


2018 ◽  
Vol 280 ◽  
pp. S35-S36
Author(s):  
Elena Narcisa Pogurschi ◽  
Monica Paula Marin ◽  
Dumitru Dragotoiu ◽  
Tomita Dragotoiu

1962 ◽  
Vol 59 (3) ◽  
pp. 387-392 ◽  
Author(s):  
T. Z. Nowakowski

Italian rye-grass given ammonium sulphate or sodium nitrate at 56 or 112 lb. N/acre was analysed for total nitrogen, soluble nitrogen (non-protein-nitrogen) and soluble carbohydrates.Ten days after applying fertilizer the differences in total-N between the grass receiving 56 and grass receiving 112 lb. N/acre were very small. Total-N in grass decreased with growth, but the effect of the rate of nitrogen on total-N increased. At first the grass given sodium nitrate contained more soluble nitrogen than grass given ammonium sulphate, the difference being greater at 56 lb. N/acre; soluble nitrogen decreased with increasing growth. Ten days after applying fertilizer, the nitrate-N content of grass was very high (ranging from 0·1 to 0·9% in the D.M.) and it gradually decreased. At both levels of nitrogen application, grass given sodium nitrate contained much more nitrate-N than grass given ammonium sulphate. Forty days after applying nitrogen the nitrate-N contents of grass which received 56 and 112 1b. N/acre as ammonium sulphate were 0·039 and 0·222% of the dry matter, respectively; the grass supplied with sodium nitrate gave values of 0·082 and 0·438%.Total soluble carbohydrates in the grass were small early in growth and gradually increased. Nitrogen dressings had little effect on the content of soluble sugars (glucose + fructose + sucrose) but greatly decreased the fructosan. The pattern of changes in the total soluble carbohydrate content followed that in fructosan content. Early in growth, the total soluble carbohydrate/crude protein ratio was very small in grass from all treatments except the ‘control’. This ratio increased with growth and at the last sampling was 2·13 in grass receiving no nitrogen, and in grass supplied with 56 and 112 lb. N/acre as ammonium sulphate it was 1·44 and 0·72 respectively; the corresponding figures for grass receiving sodium nitrate were 1·13 and 0·66. The total soluble carbohydrate carbon/soluble nitrogen ratio in grass with no nitrogen was 18 at the first sampling and it increased gradually, reaching 70 at the last sampling. This ratio was considerably less with all nitrogen treatments than with ‘control’. The values obtained with 112 lb. N/acre were less than those obtained with 561b./acre, irrespective of the form of nitrogen used.The relationship between the soluble carbohydrate carbon content and the soluble nitrogen in grass is illustrated graphically and discussed.


2012 ◽  
Author(s):  
Wan Mohtar Wan Yusoff ◽  
Mohd. Mazmira Mohd. Masri ◽  
Choy Mei Chan

Kesan penambahan ammonium sulfat, (NH4)2SO, (sebagai sumber nitrogen bukan organik tunggal) terhadap pensporaan Bacillus thuringiensis subsp. aizawai strain SN2 dalam kultur kelompok telah dikaji. Peratus spora tertinggi (76 %) dikesan selepas 96 j pengkulturan dalam medium yang mengandungi 1.5 gL-1 (NH4)2SO4. Peratus spora tertinggi sebanyak 82 % juga dikesan dalam sampel 96 j apabila medium yang mengandungi 1.5 gL-1 (NH4)2SO4 ditambah dengan 3.0 gL-1 (NH4)2SO4 pada jam keenam selepas mula fermentasi. Peningkatan peratus spora didapati tidak berkaitan dengan jumlah kandungan nitrogen tetapi berkaitan dengan masa penambahan sumber nitrogen. Kata kunci: Bacillus thuringiensis subsp. aizawai strain SN2, masa penambahan nutrien, kultur kelompok The effect of ammonium sulphate (as the sole inorganic nitrogen source) on the sporulation of Bacillus thuringiensis subsp. aizawai strain SN2 was investigated in batch fermentation. The spore percentage of 76 % was achieved at 96 h after inoculation into a medium containing initial cencentration of 1.5 gL-1 ammonium sulphate. In another experiment, the maximum spore percentage of 82 % was obtained after 96 h inoculation in a medium with initial concentration of 1.5 gL-1 (NH4)2SO4 followed by an addition of 3.0 gL-1 (NH4)2SO4 after 6 h of fermentation. The increase in Bacillus thuringiensis spore percentage was not a function of total nitrogen content in the medium but was a function of the time nitrogen being added. Key words: Bacillus thuringiensis subsp. aizawai strain SN2, timing addition, batch fermentation


1973 ◽  
Vol 40 (3) ◽  
pp. 323-328
Author(s):  
G. Tanev

SummaryA 23-week trial was conducted using 4 groups each of 6 ewes to study the effects on milk yield and composition of substituting non-protein nitrogen (NPN) for 75 % of the dietary nitrogen. The control group received a winter ration which included silage followed by a summer ration containing lucerne. The test groups received substitutes of urea, ammonium sulphate, or urea+(NH4)2SO4. The milk yield of the test groups was lower (P< 0·01) than that of the control group. Depression of the fat content of the milk reached statistical significance (P< 0·01) in the groups receiving (NH4)2SO4and urea+(NH4)2SO4. In comparison with the control group, the protein content of the milk was greater in the group receiving urea and smaller in the other 2 test groups. The milks of the test groups had lower amounts of essential amino acids in the free state and higher amounts of non-essential amino acids than did the control milk. Milk-clotting time was increased in the test groups; the increase was greatest (P< 0·01) for the group receiving (NH4)2SO4and least (P< 0·05) for that receiving urea.


1959 ◽  
Vol 12 (4) ◽  
pp. 479 ◽  
Author(s):  
RG Wake

The liberation of non-protein nitrogen, soluble in 12 per cent. Trichloroacetic acid, from various milk protein fractions by rennin (0,1 fLg rennin NJml) at pH 6�7 and 25�C is examined. 1� 0 per cent. nitrogen is rapidly released from whole casein, 3� 4 per cent. from second-cycle casein-fraction S, and 6� 7 per cent. from K-casein. None is split from other milk protein fractions under the same conditions.


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