Provision of high nitrogen roughage to sheep as a supplement to low nitrogen roughage

1975 ◽  
Vol 15 (74) ◽  
pp. 342 ◽  
Author(s):  
GE Robards ◽  
GR Pearce
Keyword(s):  
Poor Response ◽  
Control Group ◽  
In Vitro Digestibility ◽  
High Nitrogen ◽  
Wool Production ◽  
Wool Growth ◽  
Ad Libitum ◽  
Low Nitrogen ◽  
Basal Ration

Three feeding experiments and a digestibility study are reported in which the effects of supplementing low nitrogen roughages with roughages of high nitrogen content were studied. In each feeding experiment the effect of varying the frequency of supplementation was examined. During the first experiment intake of lucerne hay was greater (P < 0.05) than the intake of oaten hay and higher wool growth rates and liveweight changes were recorded. When the oaten and lucerne hays were alternated at intervals of one, two, three or four days, the average intake and rate of liveweight change was intermediate between the two previous levels, but the rate of wool production was similar to when lucerne hay was offered alone. There were no differences in total intake, clean wool production or liveweight change due to the frequency with which the two rations were alternated. The second experiment involved the feeding of a fixed quantity of lucerne hay with a. five times as much oaten hay or b. ad libitum oaten hay. The lucerne hay was given daily and every second, fourth and eighth days, and there was an unsupplemented control group. During the restricted feeding period frequent supplementation resulted in higher liveweight gain but there was no effect on wool growth. When oaten hay was provided ad libitum the poor response to supplementation and the large variation in intake within groups resulted in no significant differences in wool production or liveweight change between groups. The results of the third experiment indicate that the digestibility or degree of lignification of the basal ration affects the likelihood of a response to supplementation. It was shown that frequent supplementation of pasture hay resulted in higher intake, liveweight and wool production. On the other hand, substitute feeding with little change in animal production occurred when lucerne hay was offered to similar sheep consuming a basal ration of oaten hay which had a higher level of fibre and a lower in vitro digestibility than the pasture hay.

Supplementary feeding of Merino wethers grazing weed-free stubble pastures

1981 ◽  
Vol 21 (110) ◽  
pp. 272 ◽  
Author(s):  
LG Butler
Keyword(s):  
Drinking Water ◽  
Field Trial ◽  
Compensatory Growth ◽  
Supplementary Feeding ◽  
Control Group ◽  
Apparent Digestibility ◽  
Wool Production ◽  
Wool Growth ◽  
Wheat Stubble

A field trial was conducted in the Wimmera region of Victoria to investigate the effect of several supplements on 4-year-old Merino wethers grazing a weed-free wheat stubble for 3 months, The four treatments were: a control (no supplement), urea/molasses block, 100 g/head day-1 of lupin grain, and 2 g urea plus 0.5 g Na2SO4 l-1 in the drinking water. Twenty sheep per treatment were used, and liveweight change and wool growth were measured. Although all groups lost weight, the lupin group lost only 0.5 kg per head over the 3 months of the trial, and was 3.8 kg heavier (P <0.01) than the control group, while the urea/molasses group was 2.2 kg heavier (P <0.01) than the control group. There was no effect of the urea plus sulfur supplement in the drinking water on liveweight or wool production. The urea/molasses block did not affect wool production but lupin grain stimulated wool production by 0.08 kg (P <0.05). The sheep were weighed and shorn four and a half months after the trial (August), during which time all groups grazed together on green feed. The experimental liveweight differences had disappeared, apparently due to compensatory growth. In addition, a digestibility trial was conducted in an attempt to explain some of the field results. The treatments were designed to simulate those used in the field. Lupin grain stimulated intake by 129 g day-1 (P <0.05) but had no effect on apparent digestibility of straw. The treatment simulating the urea/ molasses block (urea plus sulfur plus sucrose in the drinking water) stimulated intake by 159 g day-l (P <0.05) and tended to increase digestibility (P > 0.05). It was concluded that supplementary feeding of Merino wethers grazing weed-free stubble is unlikely to be an economic farm practice.

scholarly journals mTOR Inhibition Rejuvenates the Aging Gingival Fibroblasts through Alleviating Oxidative Stress

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Yiru Xia ◽  
Mengjun Sun ◽  
Yufeng Xie ◽  
Rong Shu
Keyword(s):  
Oxidative Stress ◽  
Poor Response ◽  
Control Group ◽  
Oxygen Species ◽  
Gingival Fibroblasts ◽  
Periodontal Pathogens ◽  
Mtor Inhibition ◽  
Mechanistic Target Of Rapamycin ◽  
Beta Galactosidase

The aging periodontium may be vulnerable to periodontal pathogens and poor response to inflammation and susceptible to tumorigenesis. Human gingival fibroblasts (hGFs) through continuously replicative culture served as an in vitro surrogate for aging. To investigate the effects of the mechanistic target of rapamycin (mTOR) inhibition on the aging gingiva, we stimulated the high-passage hGFs with rapamycin (20 nmol/L) for 3 days and 30 days. The cellular and biological changes were examined by immunofluorescence, real-time PCR, ELISA, Western blotting, and flow cytometry. The data demonstrated that the inhibition of mTOR signaling led to fewer senescence-associated beta-galactosidase- (SA-β-Gal-) positive cells, delayed the onset of senescence, preserved the capability of proliferation, and lowered the expression levels of relevant senescence-associated markers, such as p16INK4a, p21CIP1a, interleukin-6 (IL-6), and IL-8. In addition, when infected by prominent periodontal pathogens, Porphyromonas gingivalis (ATCC 33277), rapamycin-pretreated groups decreased the expression of inflammatory cytokines (IL-6 and IL-8) compared with the control group. mTOR inhibition upregulated the gene expression of antioxidant components (Cat, Sod2, and Prdx3; P<0.05) and consequently neutralized the excessive reactive oxygen species (ROS). In conclusion, our results indicated that mTOR inhibition might rejuvenate the aging gingiva to some extent and relieve inflammation through eliminating oxidative stress.

Formaldehyde as a silage additive. II. The chemical composition and nutritive value of lucerne hay, lucerne silage, and formaldehyde and formic acid-treated lucerne silages

1973 ◽  
Vol 24 (6) ◽  
pp. 939 ◽  
Author(s):  
SC Valentine ◽  
DC Brown
Keyword(s):  
Formic Acid ◽  
Dry Matter ◽  
Nutritive Value ◽  
Similar Degree ◽  
Fresh Weight ◽  
Wool Production ◽  
Wool Growth ◽  
Ad Libitum ◽  
Ad Libitum Intake

Formaldehyde-treated silage, formic acid-treated silage, formaldehyde-formic acid-treated silage, untreated silage, and lucerne hay were made from a lucerne sward and offered to Merino wethers. The formaldehyde was applied at a rate of 0.9 % of the weight of the dry matter and formic acid at 0.5 % of the fresh weight of the lucerne. Formaldehyde-treated silage and formaldehyde-formic acid-treated silage had significantly lower concentrations of ammonia nitrogen and total and individual organic acids than untreated silage. Formic acid-treated silage had a similar degree of fermentation to untreated silage, but more acetic acid and less lactic acid were produced than in untreated silage. However, when formic acid was applied in combination with formaldehyde, the ensiling fermentation was inhibited more than when formaldehyde was applied alone. There was some apparent protection of protein by the formaldehyde treatment. Treatment with formic acid significantly increased the in vivo digestibility of both nitrogen and dry matter, but did not increase ad libitum intake or wool growth by sheep offered this silage. Compared to untreated silage, treatment with formaldehyde significantly reduced the in vivo digestibility of nitrogen, produced a non-significant increase in intake, and significantly increased wool growth. The treatment of lucerne with both formaldehyde and formic acid significantly increased in vivo digestibility of dry matter, ad libitum intake, and wool growth compared with untreated silage. The treatment of lucerne with a mixture of formaldehyde (0.9% of the dry matter) and formic acid (0.5% of the fresh weight) was a suitable method of controlling the ensiling fermentation in order to increase the ad libitum intake of lucerne silage by sheep and their subsequent wool production. However, the intake and wool production of sheep offered such silage was still significantly less than that of sheep offered lucerne hay.

EFFECT OF BARLEY SUPPLEMENT TO THE RATION OF DONOR ANIMALS USED IN IN VITRO DIGESTIBILITY DETERMINATION

1970 ◽  
Vol 50 (2) ◽  
pp. 265-267 ◽  
Author(s):  
F. W. CALDER
Keyword(s):  
Hordeum Vulgare ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Barley Grain ◽  
In Vitro Digestibility ◽  
Hordeum Vulgare L ◽  
Alfalfa Hay ◽  
Medicago Sativa L ◽  
Ad Libitum

Rumen fluid from two sheep was used to determine in vitro dry matter digestibilities of 25 samples each from alfalfa hay (Medicago sativa L.) and barley grain (Hordeum vulgare L.). The sheep were fed either a good quality hay ad libitum and 1 kg rolled barley per day, or only the hay ad libitum, in a switchback design. The in vitro dry matter digestibilities of both alfalfa hay and barley were lower (P < 0.01) when the donor animal was fed hay and barley than when fed only hay.

scholarly journals Comparison Effects of Ruminal Crabtree-Negative Yeasts and Crabtree-Positive Yeasts for Improving Ensiled Rice Straw Quality and Ruminal Digestion Using In Vitro Gas Production

2020 ◽  
Vol 6 (3) ◽  
pp. 109 ◽  
Author(s):  
Chanon Suntara ◽  
Anusorn Cherdthong ◽  
Suthipong Uriyapongson ◽  
Metha Wanapat ◽  
Pin Chanjula
Keyword(s):  
Rice Straw ◽  
Dry Matter ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Control Group ◽  
In Vitro Digestibility ◽  
Pichia Kudriavzevii ◽  
Yeast Strains ◽  
In Vitro Gas Production

The objective of this study was to compare the effects of Crabtree-negative ruminal yeast and Crabtree-positive yeast in ensiled rice straw (RS) on the ensilage quality, nutritive value, and microorganism composition, including the evaluation of the ensiled RS using the in vitro gas production technique. The experiment was conducted in a 4 × 3 factorial arrangement in a randomized complete design. Factor A was yeast species with no inoculant, Crabtree-negative yeasts (Pichia kudriavzevii KKU20 and Candida tropicalis KKU20), and Crabtree-positive yeast (Saccharomyces cerevisae), whereas factor B was ensilage times (7, 14, and 21 days). The rate of growth was revealed to be lower in Crabtree-positive yeasts than the other Crabtree-negative yeast strains (p < 0.01). RS ensiled with S. cerevisiae showed decreased dry matter (DM) content by 9.0% when compared to the sample without a yeast inoculant. In addition, organic matter (OM) content was greater (p < 0.01) for P. kudriavzevii KKU20 than C. tropicalis KKU20 and without an inoculant. Neutral detergent fiber (NDF) content was significantly decreased (p < 0.01) by yeast inoculants by about 2.75% when compared to the control group. Lactic acid bacteria (LAB) and aerobic bacteria were low (p < 0.05) when yeasts were added. However, no interaction was found between yeast and ensilage times on the quality of ensiled RS (p > 0.05). The P. kudriavzevii KKU20 addition was associated with the highest value (p < 0.01) of gas produced—an insoluble fraction (b), potential extent of gas production (a + b), and cumulative gas production at 96 h—when compared with S. cerevisiae or the control group. The highest in vitro dry matter digestibility and in vitro neutral detergent fiber digestibility was observed in RS ensiled with P. kudriavzevii KKU20 for 14 days (p < 0.01, p < 0.05). The maximum total volatile fatty acids (VFAs) at 4 and 8 h of incubation and the mean value were observed in RS ensiled with P. kudriavzevii KKU20 (p < 0.01). RS ensiled with all yeast strains showed an increased propionate concentration at 8 h (p < 0.01). In conclusion, ensiling RS with isolated Crabtree-negative ruminal yeasts could benefit feed digestion and in vitro gas production more than Crabtree-positive yeast does. P. kudriavzevii KKU20, an isolated Crabtree-negative ruminal yeast used to treat RS, had the highest potential for increasing cumulative gas production and enhancing in vitro digestibility.

A study of Merino sheep grazing a cotton-bush (Kochia aphylla)–grassland (Stipa variabilis–Danthonia caespitosa) community on the riverine plain

1968 ◽  
Vol 19 (6) ◽  
pp. 947 ◽  
Author(s):  
JH Leigh ◽  
AD Wilson ◽  
WE Mulham
Keyword(s):  
In Vitro Digestibility ◽  
Stocking Rate ◽  
Merino Sheep ◽  
Dietary Crude Protein ◽  
Wool Growth ◽  
And Performance ◽  
Main Component ◽  
Stocking Rates ◽  
Low Rate

A study was made of Merino sheep continuously grazing a cotton-bush (Kochia aphylla R.Br.)–grassland (Stipa variabilis Hughes–Danthonia caespitosa Gaudich.) community over a 2 year period. Observations were carried out at two stocking rates (0.75 and 0.375 sheeplacre) and comparisons were made with plots from which K. aphylla had been mechanically removed. The aim of the study was to define the contribution made by K. aphylla (a drought-resistant chenopodiaceous shrub) to animal production and to examine the effects of the seasonal variation in composition, availability, and food value of the pasture on animal behaviour and performance. At the low rate of stocking K. aphylla contributed little to the diet of the sheep (max. 2% in autumn) despite its being at no time less than 20% of the forage available. At the high stocking rate its maximum contribution to the diet was 7% in late autumn. The major constituents of the diet in spring were the annuals Hedypnois rhagadioloides (L.) Willd. and Hypochoeris spp. and in summer the perennials Danthonia caespitosa and Kochia pentagona R.H. Anderson. D. caespitosa was also the main component during autumn at the low stocking rate but at the high stocking rate it was replaced in the diet by a high proportion of dead matter and Medicago burr. Seasonal variations in wool growth were greater at the high stocking rate than at the low rate, the lowest production occurring in autumn. Body weight and wool growth were not significantly altered by the presence of K. aphylla at either of the stocking rates. The lowest recorded value for dietary crude protein was 8.8%. Wool growth was closely related to the in vitro digestibility of the forage eaten and to the availability of green feed within the pasture.

scholarly journals Combining Crude Glycerin with Chitosan Can Manipulate In Vitro Ruminal Efficiency and Inhibit Methane Synthesis

Animals ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 37
Author(s):  
Anuthida Seankamsorn ◽  
Anusorn Cherdthong ◽  
Metha Wanapat
Keyword(s):  
Volatile Fatty Acids ◽  
Gas Production ◽  
Nutrient Digestibility ◽  
Crude Glycerin ◽  
Control Group ◽  
In Vitro Digestibility ◽  
Ruminal Fermentation ◽  
Ch4 Production ◽  
In Vitro Gas Production

It was hypothesized that the combination of glycerin and chitosan improves ruminal fermentation efficiency via an enhanced propionate (C3) and reduces in vitro CH4 production. This was explored through in vitro gas production with substrates containing crude glycerin, which replaced cassava chips in the studied ration. The experimental design was organized following a 3 × 3 factorial in completely randomized design and the arrangement of treatments were different levels of crude glycerin supplementations 0, 10.5, and 21% of total mixed ration (TMR) and chitosan levels were added at 0, 1, and 2% dry matter (DM) of substrate. Then, 0.5 g of TMR substrates were added into 40 mL bottles, together with respective doses of chitosan and then incubated at 39 °C. The dietary treatments were performed in three replicates within the incubation, and incubations were repeated on three separate days (runs). No interactions were found between crude glycerin and chitosan doses in terms of theoretical maximum of asymptotic gas production (b), rate of gas production (c), the discrete lag time prior to gas production (L), or the cumulative gas production at 96 h of incubation (p > 0.05). Cumulative gas production at 96 h of incubation was similar among the doses of crude glycerin and levels of chitosan, which ranged from 64.27 to 69.66 mL/g DM basis of substrate (p > 0.05). The concentration of ruminal NH3-N after 2 and 4 h of incubation ranged from 14.61 to 17.10 mg/dL and did not change with the addition of crude glycerin with chitosan (p > 0.05). The concentration of CH4 after 2 h of incubation did not change among treatments (p > 0.05), whereas after 4 h of incubation, CH4 synthesis was significantly reduced by enhancing doses of crude glycerin and chitosan (p < 0.05). The combination of 21% of crude glycerin in TMR with 2% chitosan depressed CH4 production as much as 53.67% when compared to the non-supplemented group. No significant crude glycerin and chitosan interaction effect was detected for in vitro digestibility of nutrients after incubation for 12 and 24 h using the in vitro gas production technique (p > 0.05). In addition, no significant changes (p > 0.05) were observed in total volatile fatty acids, acetate (C2) or butyrate content among treatments and between the main effects of crude glycerin with chitosan. At 4 h of incubation, ruminal C3 content and the C2 to C3 ratio changed significantly when crude glycerin and chitosan was added (p < 0.05). The 21% crude glycerin incorporate into TMR, in combination with 2% additional chitosan, increased C3 content by 26.41%, whereas the ratio of C2 to C3 was reduced by 31% when compared to the control group. Propionate concentration increased by 11.75% when increasing levels of chitosan at 2% of substrate, whereas the C2 to C3 ratio decreased by 13.99% compared to the 0% chitosan group. The inclusion of crude glycerin at 21% in TMR diets with chitosan supplementation at 2% enhanced ruminal propionate concentration and reduced methane production without causing any detrimental effect on the gas kinetics or nutrient digestibility.

scholarly journals Combination of Xylan Depolymerizing and Debranching Enzymes Improves Digestion, Growth Performance and Intestinal Volatile Fatty Acid Profile of Piglets

2021 ◽  
Author(s):  
Weiwei Wang ◽  
Dawen Zheng ◽  
Zhenzhen Zhang ◽  
Qingyun Cao ◽  
Hui Ye ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Growth Performance ◽  
Fatty Acid Profile ◽  
Volatile Fatty Acid ◽  
Feruloyl Esterase ◽  
Control Group ◽  
In Vitro Digestibility ◽  
Final Body Weight ◽  
Debranching Enzymes

This study was aimed to investigate the effect of xylan depolymerizing enzyme namely endo-xylanase (Xyn) combined with debranching enzymes namely arabinofuranosidase (Afd) and feruloyl esterase (FE) on digestion, growth performance and intestinal volatile fatty acid profile of piglets. The in vitro experiments were firstly conducted to examine the enzymological properties of Xyn, Afd and FE, the synergy among these enzymes, together with the effect of combination of these enzymes on digestion of piglet diet. The in vivo experiment was then implemented by allocating 270 35-d-old postweaning piglets into 3 treatment groups: control group, Xyn group and (Xyn+Afd+FE) group. Each group had 6 replicates (15 piglets/replicate). The results revealed a satisfying thermostability and pH stability of Xyn, Afd and FE. Combination of Xyn, Afd and FE had a superiority (P &lt; 0.05) over Xyn alone and its combination with Afd or FE in promoting degradation of different bran fibers rich in arabinoxylan (Abx). Treatment with combination of Xyn, Afd and FE had advantages over Xyn alone to induce increasing trends (P &lt; 0.10) of in vitro digestibility of dietary nutrients (dry matter, crude protein, crude ash and gross energy) and piglet growth performance (average daily gain, final body weight and feed efficiency), concurrent with a reduction (P &lt; 0.05) of diarrhea rate and increases (P &lt; 0.05) in cecal acetic acid, butyric acid and total volatile fatty acids concentrations as well as pH value of piglets. Collectively, combination of Xyn, Afd and FE was efficient in benefiting degradation of Abx in brans, as well as improving digestion, growth performance and intestinal volatile fatty acid profile of piglets.

scholarly journals Rhodaneses Enzyme Addition Could Reduce Cyanide Concentration and Enhance Fiber Digestibility via In Vitro Fermentation Study

Fermentation ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 207
Author(s):  
Chanadol Supapong ◽  
Anusorn Cherdthong
Keyword(s):  
Volatile Fatty Acids ◽  
Potassium Cyanide ◽  
Insoluble Fraction ◽  
Ammonia Nitrogen ◽  
Gas Production ◽  
Nutrient Digestibility ◽  
Control Group ◽  
In Vitro Digestibility ◽  
Ruminal Fermentation

The use of cyanide-containing feed (HCN) is restricted because it causes prussic acid poisoning in animals. The objective of this study was to see how adding rhodanese enzyme to an HCN-containing diet affected gas dynamics, in vitro ruminal fermentation, HCN concentration reduction, and nutrient digestibility. A 3×4 factorial arrangement in a completely randomized design was used for the experiment. Factor A was the three levels of potassium cyanide (KCN) at 300, 450, and 600 ppm. Factor B was the four doses of rhodanese enzyme at 0, 0.65, 1, and 1.35 mg/104 ppm KCN, respectively. At 96 h of incubation, gas production from an insoluble fraction (b), potential extent (omit gas) (a + b), and cumulative gas were similar between KCN additions of 300 to 450 ppm (p > 0.05), whereas increasing KCN to 600 ppm significantly decreased those kinetics of gas (p < 0.05). Supplementation of rhodanese enzymes at 1.0 to 1.35 mg/104 ppm KCN enhanced cumulative gas when compared to the control group (p < 0.05). Increasing the dose of rhodanese up to 1.0 mg/104 ppm KCN significantly increased the rate of ruminal HCN degradation efficiency (DE) by 70% (p < 0.05). However, no further between the two factors was detected on ruminal fermentation and in vitro digestibility (p > 0.05). The concentration of ammonia-nitrogen (NH3-N) increased with increasing doses of KCN (p < 0.05), but remained unchanged with varying levels of rhodanese enzymes (p > 0.05). The in vitro dry matter digestibility (IVDMD) was suppressed when increasing doses of KCH were administered at 600 ppm, whereas supplementation of rhodanese enzymes at 1.0–1.35 mg/104 ppm KCN enhanced IVDMD (p < 0.05). Increasing doses of KCN affected reduced total volatile fatty acids (TVFA) concentration, which was lowest when 600 ppm was added (p < 0.05). Nevertheless, the concentration of TVFAs increased when rhodanese enzymes were included by 1.0–1.35 mg/104 ppm KCN (p < 0.05). Based on this study, it could be concluded that supplementation of rhodaneses enzyme at 1.0–1.35 mg/104 ppm KCN could enhance cumulative gas, digestibility, and TVAF, as well as lowering ruminal HCN concentration.

Nutrient partitioning in Merino rams with different wool growth rates

1994 ◽  
Vol 59 (1) ◽  
pp. 55-60 ◽  
Author(s):  
P. B. Cronjé ◽  
M. Smuts
Keyword(s):  
Growth Rate ◽  
Production Potential ◽  
Physiological Basis ◽  
Wool Production ◽  
Available Nutrients ◽  
Wool Growth ◽  
Nutrient Partitioning ◽  
Ad Libitum ◽  
Selection For ◽  
The Cost

AbstractThe aim of this experiment was to determine whether there is any physiological basis for concerns that selection for fleece mass may decrease fitness by increasing the partitioning of nutrients to wool at the expense of other body functions.Forty-five Merino rams (24 months old) were given food at an ad libitum and a maintenance level of nutrition. Animals were grouped into high, average or low clean fleece growth-rate categories on the basis of measurements made during the ad libitum feeding period. During ad libitum feeding, high producers grew proportionately 0·42 more clean fleece, and deposited proportionately 0·36 more energy and 0·42 more nitrogen (N) as greasy fleece than average producers. This was achieved by partitioning a higher proportion of N consumed to wool (0·08 v. 0·06), and not by increased energy or N retention. High producers adapted to the maintenance diet by decreasing clean wool growth rate proportionately by 0·32. There were no differences between high and average wool producers in clean wool growth rate or the percentage of N or energy intake partitioned to wool at the maintenance level of feeding. Although there were no differences in initial live mass between groups, low producers gained proportionately 0·45 more live mass during the experiment than average producers.It was concluded that Merino rams of high clean-wool production potential are not more efficient, but partition more of the available nutrients to wool production at the cost of body tissue deposition.

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