Studies on seed pelleting as an aid to legume seed inoculation. 3. Survival of Rhizobium applied to seed sown into hot, dry soil

1970 ◽  
Vol 10 (47) ◽  
pp. 739 ◽  
Author(s):  
J Brockwell ◽  
LJ Phillips

Seed of four legume species with various forms of lime pelleting and inoculation was sown into hot, dry soil at Katherine, Northern Territory, where it lay dormant for 7-9 weeks before germination commenced. Seedling nodulation was used as the index of inoculant survival. Where the seed was sown at a shallow depth, Rhizobium survival was poorer than on seed sown more deeply; this was attributed to the higher temperatures near the soil surface. Rhizobia survived best in those treatments in which peat inoculant was incorporated within the pellet. Rhizobium meliloti applied to Medicago sativa seed showed a high degree of tolerance of the conditions and seedling nodulation exceeded 90 per cent in several instances. Nodulation of Trifolium pratense and T. rueppellianum never exceeded 50 per cent and little nodulation occurred with Lotus pedunculatus. It is concluded that lime-pelleted Medicago seed with peat inoculant incorporated within the pellet can be sown into hot, dry soil with a strong expectation that the inoculant will survive and the seedlings nodulate.

1970 ◽  
Vol 10 (45) ◽  
pp. 455 ◽  
Author(s):  
J Brockwell ◽  
RDB Whalley

Seed of Medicago truncatula and Medicago littoralis, with and without lime pelleting and inoculation, was sown dry into medic-free, Rhizobim meliloti-free soils at 12 sites in the Western Division of New South Wales. At the different sites, periods between 1 and 88 days elapsed before germinating rain fell. Seedling nodulation was used as the index of inoculant survival. Nodulation was variable, but the variation was independent of the time between sowing and germination. Best nodulation occurred in treatments in which peat inoculant was incorporated within a lime pellet around the seed. These treatments were superior to others where broth inoculant was incorporated in the pellet or where peat inoculant was applied externally to pelleted or unpelleted seed. Nodulation was improved by incorporating an increased level of peat inoculant in the pellet. In storage, a very low survival rate was recorded in all treatments except those incorporating peat inoculant within the pellet. The results are discussed in relation to the practicalities of pre-inoculation techniques and of sowing inoculated medic seed into dry seed beds.


2007 ◽  
Vol 19 (4) ◽  
pp. 1-13
Author(s):  
Manaf Ezzldien Al-Sabbagh ◽  
Jasim Khalaf Shallal ◽  
Sabah Hussein Ali

Weed Science ◽  
1978 ◽  
Vol 26 (1) ◽  
pp. 32-36 ◽  
Author(s):  
J. Segura ◽  
S. W. Bingham ◽  
C. L. Foy

The phytotoxicity of glyphosate [N-(phosphonomethyl) glycine] on seeds (protected or non-protected with soil) and seedlings of Italian ryegrass(Lolium multiflorumLam.) and red clover(Trifolium pratenseL.) was studied in the greenhouse. Percent germination of Italian ryegrass seeds covered with soil was significantly reduced with glyphosate applied at 4 kg/ha. The direct treatment over the seeds on the soil surface was more toxic, reducing the number of established seedlings at dosages of 3 and 4 kg/ha. In the two red clover seed treatments, germination was significantly reduced with 2 kg/ha of herbicide. Even though germination was reduced at intermediate rates of glyphosate, shoot growth and tillering were improved and yields of dry weight were equivalent to that of untreated plants. Postemergence applications of glyphosate were phytotoxic to both species with the effects becoming more pronounced at increasing dosages. However, at equal dosage, red clover was less susceptible than Italian ryegrass. The patterns of foliar absorption and translocation of14C from14C-glyphosate was similar in Italian ryegrass and red clover.14C was translocated throughout the plants and exhibited apoplastic and symplastic movement.


1957 ◽  
Vol 37 (2) ◽  
pp. 108-112
Author(s):  
F. L. Banham ◽  
R. H. Handford

Emulsions of dieldrin, aldrin, isodrin, toxaphene and chlordane applied to the soil surface and incorporated to a depth of about 4 inches proved highly effective in controlling the red-back cutworm, Euxoa ochrogaster (Guen.) when tested in asparagus fields in the interior of British Columbia in the summer of 1953 and 1954. In 1953 aldrin emulsion mixed with the soil was much more effective than when it was left on the soil surface, Bran bait containing paris green, although giving fairly satisfactory control, was less effective and slower in action than the emulsions. In 1952, dieldrin, aldrin, and isodrin dusts, applied to the soil surface, were superior to and faster in action than bran baits containing aldrin or endrin; all of the 1952 treatments were apparently slower in action in dry soil than in relatively moist soil. A survey of asparagus fields treated by growers in 1953 but not in 1954 indicated that aldrin emulsion, mixed with the soil at about 4 lb. of toxicant per acre, protects asparagus for at least two years.


1991 ◽  
Vol 42 (5) ◽  
pp. 893 ◽  
Author(s):  
DC Edmeades ◽  
FPC Blamey ◽  
CJ Asher ◽  
DG Edwards

Ten temperate pasture legumes inoculated with appropriate rhizobia were grown for 31 days in flowing solution culture. Solution ionic strength was approximately 2700 8M and contained inorganic nitrogen (150 , 8M NO3-) only at the commencement of the experiment. Solution pH was maintained at 4.5, 5.0, 5.5 and 6.0. Also, five aluminium (Al) treatments were imposed, with nominal Al concentrations of 0, 3, 6, 12 and 24 8M (2.5, 7.1, 8.3, 11.2 and 24.7 8M Al measured) at pH 4.5. Solution pH <6 . 0 markedly reduced total dry mass (TDM) in all cultivars of white clover (Trifolium repens) cvv. 'Grasslands Pitau, Huia, G18 and Tahora' and red clover (Trifolium pratense) cvv. 'Grassland Turoa and Pawera', and to a lesser extent in the two subterranean clover (Trifolium subterraneum) cvv. 'Tallarook and Woogenellup'. In contrast, solution pH had no effect on the growth of Lotus corniculatus cv. Maitland, while Lotus pedunculatus cv. Maku grew best at pH 4.5. Lotus pedunculatus cv. Maku grew best in solution where the sum of the activities of the monomeric Al species {Alm} was maintained at 5.9 8M. The growth of all other species was decreased with Al in solution, a 50% reduction in TDM being associated with c. 6 8M {Alm] for white clover and subterranean clover, and c. 3 8M in red clover and Lotus corniculatus cv. Maitland.


1975 ◽  
Vol 15 (77) ◽  
pp. 780 ◽  
Author(s):  
J Brockwell ◽  
DF Herridge ◽  
RJ Roughley ◽  
JA Thompson ◽  
RR Gault

Samples of preinoculated legume seed were obtained from commercial outlets in southeastern Australia and compared with seed inoculated in the laboratory at currently recommended rates for the numbers of rhizobia associated with the seed and performance when grown in the field and in soil in a glasshouse. There were more than 100 times as many rhizobia on laboratory-inoculated seed as on preinoculated seed. There were no detectable rhizobia on three of the 48 samples of preinoculated seed examined, 22 other samples carried very low numbers, and all but one fell below standards derived from Australian lnoculant Research and Control Service requirements. When grown in soil containing naturally-occurring or added rhizobia, laboratory-inoculated seed was generally superior to preinoculated seed in percentage recovery of inoculant strains from nodules. The differences became greater as the size of soil populations of rhizobia increased. Where naturally-occurring rhizobia were few in number or absent nodulation was satisfactory provided that the seed carried viable rhizobia at time of sowing. The numbers of rhizobia associated with laboratory preparations of inoculated seed represented the potential standards that should be the commercial objective. The consistently lower numbers on preinoculated seed indicated that some stage (or stages) of the preinoculation process itself has a letha effect on the inoculant.


2011 ◽  
Vol 25 (2) ◽  
pp. 252-261 ◽  
Author(s):  
Silvia Fogliatto ◽  
Francesco Vidotto ◽  
Aldo Ferrero

Weedy rice is a problematic weed that infests paddy fields worldwide. Differing populations, with varying physiological and morphological traits, characterize this weed. In particular, seed dormancy makes its control difficult. The objective of this study was to evaluate the germination behavior of five Italian weedy rice populations (two awnless, two awned, and one mucronate) after exposure of seeds to different field storage conditions (flooding, burial, and dry soil surface) during winter in two sites (Grugliasco and Vercelli, Italy). Seed samples were taken from each population, storage condition, and site, every 15 d for petri dish germinability testing. The two sites displayed slightly different germination patterns, which were probably due to the differing climatic conditions. One of the awned populations showed the highest (always exceeding 80%) and fastest germination percentage in all field conditions and sites, compared with the other four populations. Although flooding promoted germination in one awnless population, it delayed germination in two others (one awned and one awnless), attaining only 20% germination after more than 100 d. In all populations, burial delayed germination, whereas seed placement on the dry soil surface enhanced it. Our study indicated that autumn tillage that promotes weedy rice seed burial should be discouraged; spring tillage that exposes seeds to the soil surface and cause their depletion should be encouraged. The tested technique of winter flooding can also improve weedy rice control, despite its varying efficacy among populations. Cycles of flooding and drying followed by spring tillage might improve weedy rice seed control.


1975 ◽  
Vol 55 (2) ◽  
pp. 555-563 ◽  
Author(s):  
H. T. KUNELIUS ◽  
UMESH C. GUPTA

Saranac alfalfa (Medicago sativa L.) was grown on Charlottetown fine sandy loam (CHFSL) and Culloden sandy loam (CSL) in the greenhouse and field. Uninoculated, inoculated with peat-based rhizobia (I), inoculated and molybdenum-treated (IMo), inoculated and lime-coated (ILC), and inoculated, lime-coated and Mo-treated (ILCMo) seeds were included. At soil pH 5.0 and 5.3 in the greenhouse, the dry weights of alfalfa ranged from 214 to 727 mg/plant in the four cuttings from the ILC and ILCMo seed. The dry weights of alfalfa from the I and IMo seed were 1.3 to 99.7% of those from the ILCMo seed. Alfalfa from the ILC and ILCMo seed was well nodulated, whereas I and IMo seed resulted in poor nodulation. The 1973 field experiment showed that ILC and ILCMo seed at soil pH 5.5 and 5.6 resulted in alfalfa dry matter (DM) yields of 4,050 and 4,830 kg/ha which were equal to DM yields from plots with a pH of 6.0 and 6.1 seeded with inoculated seed. In plots with pH 5.5 and 5.6, total DM yields from I and IMo seed were 26.0–49.7% lower than those from ILCMo seed. In 1972, seed treatments on CHFSL at pH 5.6 did not influence the DM yields of alfalfa, whereas on CSL at pH 5.2 the crop failed to establish except from ILC and ILCMo seed and in limed plots at pH 5.9. In the first cutting, high N concentrations of alfalfa tissue coincided with good growth and good nodulation. The Mo concentrations of alfalfa tissue were not influenced in a uniform manner by seed treatments and varied from 0.11 to 0.49 ppm in the first cut tissue.


Biologia ◽  
2009 ◽  
Vol 64 (2) ◽  
Author(s):  
Junyan Zhang ◽  
Genwei Cheng ◽  
Feihai Yu ◽  
Norbert Kräuchi ◽  
Mai-He Li

AbstractUnderstanding and predicting possible responses of grassland species to global change is of important meaning for adapting grassland management to a changed and changing environment. A laboratory clipping experiment was conducted to examine the interspecific responses in an ecological context of competition and environmental changes. Festuca rubra and Trifolium pratense, either in monoculture or two- and three-species mixtures, were grown in three environmental combinations (ambient and increased temperature, repetitive N supply, and simulated acid rain), respectively. After a growth time of three months, plants were clipped at the height of 1.0 cm above soil surface. Plant height and aboveground biomass prior clipping, and survival rate and regrowth (height and biomass) after clipping were analyzed. F. rubra and T. pratense responded differently in compensatory growth and competition intensity to environmental change and co-existing species. The differences in their physiological and ecological traits may account for species-dependent responses. The present study emphasizes that predicting the plant assemblage response in the face of global change requires in understanding the integrating effects of abiotic and biotic factors.


1997 ◽  
Vol 45 (4) ◽  
pp. 317-327 ◽  
Author(s):  
Yitzchak Gutterman ◽  
Shachar Shem-Tov

Groups of dry seeds of four annual plant species which occur in the Negev highlands were placed on a natural, dry or wet loess soil crust surface near Sede Boker on the Zin plateau during the autumn before the first rains, and on the first day with rain (1.15 mm). Ant nests ofMessor rugosuswere 8 to 14m from the experimental plot. The length of time it took these ants to collect the free or adhered seeds was observed. When the mucilaginous ombrohydrochoric seeds ofAnastatatica hierochuntica, Plantago coronopus, andCarrichtera annuaadhere to wet soil that remains moist, most of the seeds may have time to germinate in proper conditions before they are collected by ants. However, all but 5% of theReboudia pinnataseeds were collected within 2 h. The adhered seeds that had been moistened by wet soil crust and then dried, were collected by ants, in most cases, faster than when seeds and soil remained moist. Within 2 h none of the dry and free seeds situated on the dry soil surface remained. The first free seeds were collected after 7 min. Findings are discussed together with the mechanisms and strategies involved in seed dispersal by rain and germination of these plant species.


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