Salt tolerance of cowpea genotypes in the emergence stage

2001 ◽  
Vol 41 (1) ◽  
pp. 81 ◽  
Author(s):  
B. Murillo-Amador ◽  
E. Troyo-Diéguez ◽  
A. López-Cortés ◽  
H. G. Jones ◽  
F. Ayala-Chairez ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Vigna Unguiculata ◽  
Salt Tolerant ◽  
Root:Shoot Ratio ◽  
Salinity Effects ◽  
Class A ◽  
Class B ◽  
Early Seedling ◽  
Emergence Percentage ◽  
Germination Stage

The effect of NaCl salinity (0, 85 and 170 mmol/L) during emergence of 25 genotypes of cowpea [Vigna unguiculata (L.) Walp.] was studied under growth chamber conditions. Seed emergence percentage and rate, root:shoot ratio, and biomass per plant were affected by genotype, salinity and genotype salinity interaction; this interaction showed that salinity effects differed among genotypes. The criterion used to classify genotypes with respect to their salt tolerance was based on their germination percentages in both 85 and 170 mmol NaCl/L. One genotype was grouped into class ‘A’ (CB27) which had the highest salt tolerance at emergence, and was classified as salt tolerant. Another group consisting of Paceño, CB88, CB3, CB5, Tardón, Cuarenteño and CB46 was placed into class ‘B’; these genotypes showed total emergence percentages up to 75% in both 85 and 170 mmol NaCl/L. A third group of genotypes was placed into class ‘C’ which had the lowest emergence percentages in both 85 and 170 mmol NaCl/L. It was confirmed that salinity treatments affect the emergence of cowpea, delaying both emergence percentage and rate. We conclude that selection and classification for salt tolerance in cowpea genotypes can be successfully undertaken at early seedling stages, because the same genotypes were classified similarly during the germination stage in previous research.

scholarly journals Salinity Effects on Bentgrass Germination

HortScience ◽  
1993 ◽  
Vol 28 (1) ◽  
pp. 15-17 ◽  
Author(s):  
L.B. McCarty ◽  
A.E. Dudeck
Keyword(s):  
Salt Tolerance ◽  
Warm Season ◽  
Creeping Bentgrass ◽  
Salt Tolerant ◽  
Salinity Effects ◽  
Agrostis Tenuis ◽  
Seawater Salinity ◽  
Salt Tolerant Cultivars

Duplicate studies were conducted to determine salt tolerance during germination of eight bentgrass (Agrostis spp.) cultivars commonly used for overseeding warm-season turf species, such as bermudagrass (Cynodon spp.) putting surfaces. Bentgrass seeds were germinated on agar salinized with 0, 4000, 8000, 12,000, or 16,000 mg·liter-1, with the highest rate approaching one-half seawater salinity. Total germination decreased linearly or quadratically for specific cultivars as salinity increased. Time necessary to reach 50% germination across all salt concentrations was shortest for `Highland' colonial (Agrostis tenuis Sibth) and `Seaside' creeping (A. palustris Huds.) bentgrass (≈3.7 days); intermediate for `Kingstown' velvet (A. canina L.) and `Streaker' red top (A. alba L.) bentgrass (≈4.5 days); and longest for `Penneagle' creeping, `Penncross' creeping, `Exeter' colonial, and `Pennlinks' creeping bentgrass (≈5.3 days). Salt concentrations necessary to reduce germination to 90%, 75%, and 50% indicated that `Streaker' red top and `Seaside' creeping bentgrass were the most salt-tolerant cultivars. `Kingstown' velvet, `Exeter' colonial, and `Highland' colonial bentgrass were intermediate, while `Pennlinks', `Penncross', and `Penneagle' creeping bentgrass were the most salt-sensitive cultivars.

scholarly journals Comprehensive Evaluation of Salt Tolerance in Asparagus Germplasm Accessions (Asparagus officinalis L.) at Different Growth Stages

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Huimin Gao ◽  
Xuhong Zhang ◽  
Yuqin Liang ◽  
Lingdi Dong ◽  
Changzhi Han ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Adult Stage ◽  
Seedling Stage ◽  
Asparagus Officinalis ◽  
Growth Stages ◽  
Salt Tolerant ◽  
Aboveground Part ◽  
The Mean ◽  
Germplasm Accessions ◽  
Germination Stage

The screening and cultivation of salt-tolerant crops are becoming more and more important owing to the constant increase in the saline soil area worldwide. Asparagus (A. officinalis L.) is a highly nutritious vegetable crop and widely consumed globally for a long time; however, little research has been done on asparagus. In this study, the salt tolerance of 95 asparagus germplasm accessions was evaluated at three growth stages (germination, seedling, and adult stages) under both salt-stressed and control conditions. Results showed that the growth parameters of most germplasm accessions were obviously inhibited by salt stress. The mean value of the seed germination rate at the germination stage decreased by half under salt-stressed conditions, the mean salt-injury index at the seedling stage reached 57.68%, and the fresh weight of the aboveground part (FWA) and the dry weight of the aboveground part (DWA) decreased the most among the traits determined at the adult stage by more than 60%. Our study screened out 30, 19, and 18 tolerant germplasm accessions (including highly salt-tolerant and salt-tolerant germplasm accessions) at the germination stage, seedling stage, and adult stage, respectively. Among them, two germplasm accessions (Ji08-2 and Jx1502) were simultaneously identified to be tolerant in all three growth stages, while other germplasm accessions were tolerant only at one or two stages. Thus, the salt tolerance of asparagus has periodic characteristics and changes throughout the lifecycle, and the identification of salt tolerance at all the main growth stages facilitates adequate assessment and application of tolerant germplasm accessions.

scholarly journals Large-scale integrated evaluation of salt tolerance in japonica rice at the germination stage

2020 ◽  
Author(s):  
Xiu Jing ◽  
Ping Mi ◽  
Xianzhi Xie ◽  
Baoshan Wang
Keyword(s):  
Mathematical Model ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Large Scale ◽  
Crop Yields ◽  
Japonica Rice ◽  
Rice Cultivars ◽  
Salt Tolerant ◽  
Fresh Weight ◽  
Germination Stage

Abstract Background: Salt stress, one of the most important abiotic stresses, severely reduces crop yields. Identifying salt-tolerant rice germplasm resources at the germination stage, developing salt tolerance indicators, and cultivating salt-tolerant rice cultivars are crucial for improving rice production in saline soil.Results: We measured the germination parameters of 140 japonica rice cultivars on the 7 day after sowing (DAS) in 0 and 150 mmol L−1 NaCl. To accurately assess salt tolerance and identify reliable indicators of salt tolerance, we measured the shoot length (SL), root length (RL), root fresh weight (RFW), shoot fresh weight (SFW), total fresh weight (TFW) and salt tolerance (STI) index after 7 days of salt-stress treatment. The 140 rice cultivars were divided into four categories based on the mean MFVs: highly salt tolerant (HST: 19 cultivars), salt tolerant (ST: 74 cultivars), weakly salt tolerant (WST: 43 cultivars), and salt sensitive (SS: 4 cultivars). Based on the physiological indicators, we established a mathematical model to accurately evaluate salt tolerance in japonica rice cultivars. STI of TFW under 150 mmol L−1 NaCl treatment showed the highest correlation with salt tolerance during the germination stage.Conclusions: We determined the optimum NaCl concentration (150 mmol L−1) for evaluating salt tolerance in japonica rice at the germination stage. We identified 19 HST, 74 ST, 43 WST, and 4 SS japonica rice cultivars during the germination stage and proposed a mathematical model to evaluate salt tolerance. STI of TFW is a reliable, accurate indicator for evaluating salt tolerance in japonica rice. These findings should greatly facilitate the evaluation of japonica rice cultivars during seed germination and the breeding of salt-tolerant rice cultivars.

Evaluation of salt-tolerant germplasm and screening of the salt-tolerance traits of sweet sorghum in the germination stage

2018 ◽  
Vol 45 (10) ◽  
pp. 1073 ◽  
Author(s):  
Tonglou Ding ◽  
Zhen Yang ◽  
Xiaocen Wei ◽  
Fang Yuan ◽  
Shanshan Yin ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Sweet Sorghum ◽  
Development Process ◽  
Salt Tolerant ◽  
Comprehensive Index ◽  
Starting Point ◽  
High Salt Tolerance ◽  
Sorghum Bicolor L ◽  
Germination Stage ◽  
Selection Of

Soil salinity is one of the major abiotic stresses that reduces agricultural productivity and affects large terrestrial areas around the world. Germination is the starting point of the growth and development process of all crops, and it is severely affected by salt stress. Sweet sorghum (Sorghum bicolor (L.) Moench.) is one of the most promising crops that has a relatively high salt tolerance and biomass. However, few studies have evaluated the salt tolerance or screened the reliable evaluation traits of sweet sorghum. In this study, the membership function value of five traits was used as a comprehensive index for the evaluation and selection of salt tolerance in 300 sweet sorghum germplasms. After salt tolerance evaluation, 23 highly salt-tolerant, 38 salt-tolerant, 195 moderately salt-tolerant, 38 salt-sensitive and 6 highly salt-sensitive germplasms during the germination stage were screened. Moreover, the germination index under the 200 mM NaCl treatment showed the highest correlation with salt tolerance during the germination stage. This information can be used for effectively evaluating sweet sorghum during the germination stage. These results are important for the evaluation of the salt tolerance of sweet sorghum germplasms during the germination stage.

scholarly journals Genome-wide association and differential expression analysis of salt tolerance in Gossypium hirsutum L at the germination stage

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Yanchao Yuan ◽  
Huixian Xing ◽  
Wenguan Zeng ◽  
Jialing Xu ◽  
Lili Mao ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Candidate Genes ◽  
Upland Cotton ◽  
Differentially Expressed ◽  
Genome Wide Association ◽  
Rna Seq ◽  
Salt Tolerant ◽  
Genome Wide ◽  
Germination Stage

Abstract Background Salinity is a major abiotic stress seriously hindering crop yield. Development and utilization of tolerant varieties is the most economical way to address soil salinity. Upland cotton is a major fiber crop and pioneer plant on saline soil and thus its genetic architecture underlying salt tolerance should be extensively explored. Results In this study, genome-wide association analysis and RNA sequencing were employed to detect salt-tolerant qualitative-trait loci (QTLs) and candidate genes in 196 upland cotton genotypes at the germination stage. Using comprehensive evaluation values of salt tolerance in four environments, we identified 33 significant single-nucleotide polymorphisms (SNPs), including 17 and 7 SNPs under at least two and four environments, respectively. The 17 stable SNPs were located within or near 98 candidate genes in 13 QTLs, including 35 genes that were functionally annotated to be involved in salt stress responses. RNA-seq analysis indicated that among the 98 candidate genes, 13 were stably differentially expressed. Furthermore, 12 of the 13 candidate genes were verified by qRT-PCR. RNA-seq analysis detected 6640, 3878, and 6462 differentially expressed genes at three sampling time points, of which 869 were shared. Conclusions These results, including the elite cotton accessions with accurate salt tolerance evaluation, the significant SNP markers, the candidate genes, and the salt-tolerant pathways, could improve our understanding of the molecular regulatory mechanisms under salt stress tolerance and genetic manipulation for cotton improvement.

scholarly journals A Simple and Cost-effective Approach for Salt Tolerance Evaluation in Cowpea (Vigna unguiculata) Seedlings

HortScience ◽  
2019 ◽  
Vol 54 (8) ◽  
pp. 1280-1287
Author(s):  
Waltram Ravelombola ◽  
Jun Qin ◽  
Yuejin Weng ◽  
Beiquan Mou ◽  
Ainong Shi
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Chlorophyll Content ◽  
Plant Height ◽  
Vigna Unguiculata ◽  
Cost Effective ◽  
Seedling Stage ◽  
Leaf Biomass ◽  
Salt Tolerant ◽  
Leaf Injury

Little has been done with respect to breeding for salt-tolerant cowpea (Vigna unguiculata) cultivars despite of salt stress being a growing threat to cowpea production. Seedling stage is one the most susceptible stages to salt stress in cowpea. Establishing a streamlined methodology for rapidly screening a large number of genotypes will significantly contribute toward enhancing cowpea breeding for salt tolerance. Therefore, the objective of this study was to establish and validate a simple approach for salt tolerance evaluation in cowpea seedlings. A total of 30 genotypes including two controls (PI582468, a salt-tolerant genotype, and PI255774, a salt-sensitive genotype) were greenhouse-grown under 0 mm and 200 mm NaCl. A total of 14 above-ground traits were evaluated. Results revealed: (1) significant differences were observed in average number of dead plants per pot, leaf injury scores, relative salt tolerance (RST) for chlorophyll, plant height, and leaf and stem biomass among the 30 genotypes; (2) all PI255774 plants were completely dead, whereas those of PI582438 were fully green after 2 weeks of salt stress, which validated this methodology; (3) RST for chlorophyll content was highly correlated with number of dead plants and leaf injury scores; (4) RST for leaf biomass was moderately correlated with number of dead plants and leaf injury scores; and (5) RST in plant height was poorly correlated with number of dead plants and leaf injury scores Therefore, less number of dead plants per pot, high chlorophyll content, and less leaf injury scores were good criteria for salt tolerance evaluation in cowpea. This study provided a simple methodology and suggested straightforward criteria to evaluate salt tolerance at seedling stage in cowpea.

scholarly journals Determination of salt tolerance levels and genetic relationships of Vicia sativa cultivars using gene targeted functional markers

2022 ◽  
Vol 81 (1) ◽  
Author(s):  
Iskender Tiryaki ◽  
Nuray Isidogru
Keyword(s):  
Salt Tolerance ◽  
Genetic Relationships ◽  
Germination Rate ◽  
Principal Component ◽  
Germination Percentage ◽  
Functional Markers ◽  
Vicia Sativa ◽  
Salt Tolerant ◽  
Common Vetch ◽  
Germination Stage

The objectives of the present study were to determine salt tolerance levels of 12 different common vetch (Vicia sativa L.) cultivars at germination stage in the presence of 250 mM NaCl and to reveal genetic relationships based on gene targeted functional markers (GTFMs) associated with salt tolerance. The results revealed the presence of a significant genetic variation among the cultivars although s alt stress significantly reduced all germination parameters tested. The cultivar Ozveren was the most salt tolerant with 20.1% reduction in final germination percentage compared to control seeds while cultivars Alınoglu, Ayaz and Bakir did not germinate. The maximum delays in germination rate (G50 = 3.78 days) and synchrony (G10-90 = 3.45 days) were obtained from the cultivars Urkmez and Ozveren, respectively. The GTFMs provided a total of 53.1% polymorphism. The primers of MtSOS2 gene gave the highest numbers of alleles per primer pair while the highest polymorphism rate (77.8%) was obtained from the MtP5CS gene. The first three components of principal component analysis explained 57.63% of total variation. This study concluded that the cultivars determined to be salt tolerant and sensitive at germination stage distributed into three main clades determined by UPGMA analysis while the GTFMs associated with salt tolerance successfully determined the genetic relationships of common vetch cultivars.

scholarly journals Evaluation of Salt Tolerance at Germination Stage in Cowpea [Vigna unguiculata (L.) Walp]

HortScience ◽  
2017 ◽  
Vol 52 (9) ◽  
pp. 1168-1176 ◽  
Author(s):  
Waltram Second Ravelombola ◽  
Ainong Shi ◽  
Yuejin Weng ◽  
John Clark ◽  
Dennis Motes ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Seed Germination ◽  
Salinity Stress ◽  
Germination Rate ◽  
Correlation Coefficients ◽  
Human Consumption ◽  
Tolerance Index ◽  
Salt Tolerant ◽  
Salt Tolerance Index ◽  
Germination Stage

Cowpea is a leguminous and versatile crop which provides nutritional food for human consumption. However, salinity unfavorably reduces cowpea seed germination, thus significantly decreasing cowpea production. Little has been done for evaluating and developing salt-tolerant cowpea genotypes at germination stage. The objectives of this research were to evaluate the response of cowpea genotypes to salinity stress through seed germination rate and to select salt-tolerant cowpea genotypes. The seed germination rates under nonsalt condition and salinity stress (150 mm NaCl) were evaluated in 151 cowpea genotypes. Four parameters, absolute decrease (AD), the inhibition index (II), the relative salt tolerance (RST), and the salt tolerance index (STI) were used to measure salt tolerance in cowpea. The results showed that there were significant differences among the 151 cowpea genotypes for all parameters (P values <0.0001). The AD in germination rate was 5.8% to 94.2%; the II varied from 7.7% to 100%; the RST ranged from 0 to 0.92; and STI varied from 0 to 0.92. A high broad sense heritability (H2) was observed for all four parameters. High correlation coefficients (r) were estimated among the four parameters. PI582422, 09–529, PI293584, and PI582570 were highly salt tolerant at germination stage. In addition, genotypes from the Caribbean and Southern Asia exhibited better tolerance to salinity, whereas those from Europe and North America were the most salt-susceptible.

scholarly journals Comparing Salt Tolerance at Seedling and Germination Stages in Local Populations of Medicago ciliaris L. to Medicago intertexta L. and Medicago scutellata L.

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 526 ◽  
Author(s):  
Sonia Mbarki ◽  
Milan Skalicky ◽  
Pavla Vachova ◽  
Shokoofeh Hajihashemi ◽  
Latifa Jouini ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Salt Tolerant ◽  
Growth Physiology ◽  
Medicago Scutellata ◽  
Physiology And Biochemistry ◽  
Medicago Ciliaris ◽  
Early Seedling ◽  
Medicago Intertexta ◽  
Tolerance To Salinity

Salt stress is one of the most serious environmental stressors that affect productivity of salt-sensitive crops. Medicago ciliaris is an annual legume whose adaptation to agroclimatic conditions has not been well described. This study focused on the salinity tolerance of M. ciliaris genotypes compared to M. intertexta and M. scutellata in terms of plant growth, physiology, and biochemistry. Salt tolerance was determined at both germination and early seedling growth. Germination and hydroponic assays were used with exposing seeds to 0, 50, 100, 150, and 200 mM NaCl. Among seven genotypes of M. ciliaris studied, Pop1, 355, and 667, were most salt tolerant. Populations like 355 and 667 showed marked tolerance to salinity at both germination and seedling stages (TI ≤1, SI(FGP) > 0 increased FGP ≥ 20% and SI(DW) < 0 (DW decline ≤ 20%); at 100 mM); while Pop1 was the most salt tolerant one at seedling stages with (TI =1.79, SI(FGP) < 0 decline of FGP ≤ 40% and with increased DW to 79%); at 150 mM NaCl). The genotypes, 306, 773, and M. scutellata, were moderately tolerant to salt stress depending on salt concentration. Our study may be used as an efficient strategy to reveal genetic variation in response to salt stress. This approach allows selection for desirable traits, enabling more efficient applications in breeding methods to achieve stress-tolerant M. ciliaris populations.

scholarly journals NaCl Effects on In Vitro Germination and Growth of Some Senegalese Cowpea (Vigna unguiculata (L.) Walp.) Cultivars

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Mahamadou Thiam ◽  
Antony Champion ◽  
Diaga Diouf ◽  
Mame Ourèye SY
Keyword(s):  
Salt Tolerance ◽  
Vigna Unguiculata ◽  
Grain Legumes ◽  
Salt Tolerant ◽  
Cowpea Cultivars ◽  
Germination Process ◽  
Negative Effect ◽  
Salt Tolerant Cultivars ◽  
Protein Rich

Cowpea (Vigna unguiculata (L.) Walp.) is one of the most important grain legumes in sub-Saharian regions. It contributes to man food security by providing a protein-rich diet. However, its production is limited by abiotic stresses such as salinity. This study aims to evaluate the salt tolerance of 15 cowpea cultivars, at germination stage. The seed germination process consisted of sowing them in agarified water (8 g·L−1) supplemented with 6 different concentrations of NaCl (0, 10, 50, 100, 150, and 200 mM). Results highlighted that high salt concentrations drastically reduced germination and significantly delayed the process for all varieties. A cowpea varietal effect towards the salt tolerance was noticed. Genotypes Diongoma, 58-78, and 58-191 were more salt-tolerant cultivars while Mougne and Yacine were more salt-sensitive ones as confirmed in the three groups of the dendrogram. NaCl effects on the early vegetative growth of seedlings were assessed with a tolerant (58-191) and a susceptible (Yacine) cultivar. Morphological (length and dry biomass) and physiological (chlorophyll and proline contents) parameter measurements revealed a negative effect of high (NaCl). However, 58-191 was much more salt tolerant, and the chlorophyll and proline contents were higher than those of Yacine genotype at increasing salt concentrations.

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