Comparative proteomic analysis of drought response in roots of two soybean genotypes

2017 ◽  
Vol 68 (7) ◽  
pp. 609 ◽  
Author(s):  
Xingwang Yu ◽  
Aijun Yang ◽  
Andrew T. James
Keyword(s):  
Drought Stress ◽  
Proteomic Analysis ◽  
Molecular Mechanisms ◽  
Severe Drought ◽  
Soybean Genotype ◽  
Comparative Proteomic Analysis ◽  
Drought Tolerant ◽  
Soybean Genotypes ◽  
Soybean Leaves ◽  
Sensitive Genotype

Water deficit is a serious environmental stress during the soybean growth and production season in Australia. Soybean has evolved complex response mechanisms to cope with drought stress through multiple physiological processes. In this study, the roots of a previously identified drought-tolerant soybean genotype, G21210, and a sensitive genotype, Valder, were subjected to comparative proteomic analysis based on 2-dimensional electrophoresis, under mild or severe drought conditions. The analysis showed that the abundance of 179 protein spots significantly changed under stress. In total, 155 unique proteins were identified from these spots, among which 70 protein spots changed only in G2120 and 89 spots only in Valder, with 20 proteins changed in both soybean genotypes. Bioinformatics analysis revealed that these drought-induced changes in proteins were largely enriched in the biological function categories of defence response, protein synthesis, energy metabolism, amino acid metabolism and carbohydrate metabolism. For the drought-tolerant genotype, the differential abundance was decreased for 24 proteins and increased for 46 proteins. For the drought-sensitive genotype, the abundance was reduced for 46 proteins, increased for 40 proteins and changed differently for three proteins in mild and severe drought. The different patterns of change of these proteins in G2120 and Valder might be attributed to the difference in their drought-tolerance capacity. This study, combined with our previously reported proteomics study in soybean leaves, further clarifies the change in proteins under drought stress in different organs and provides a better understanding of the molecular mechanisms under drought stress in soybean production.

A comparative proteomic study of drought-tolerant and drought-sensitive soybean seedlings under drought stress

2016 ◽  
Vol 67 (5) ◽  
pp. 528 ◽  
Author(s):  
X. Yu ◽  
A. T. James ◽  
A. Yang ◽  
A. Jones ◽  
O. Mendoza-Porras ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Stress Proteins ◽  
Yield Reduction ◽  
Severe Drought ◽  
Protein Abundance ◽  
Differential Abundance ◽  
Soybean Seedlings ◽  
Drought Tolerant ◽  
Sensitive Genotype

Drought is a major factor limiting plant growth causing yield reduction in crops; hence the characterisation of drought tolerance and the development of drought-tolerant crop varieties have been a goal of many crop breeding programs. Using the proteomics approach, we compared the differential protein abundance of drought-tolerant and drought-sensitive soybean leaves subjected to mild or severe drought stress. Proteins were extracted and separated using two-dimensional electrophoresis. Those protein spots with significant and more than 2-fold difference in abundance, 174 in total, were further analysed and 102 proteins were positively identified. Around 38.5% of these proteins were related to energy metabolism and photosynthetic functions, followed by those associated with defence response (36.4%) and protein metabolism (25.2%). Severe drought resulted in a greater number of proteins with differential abundance. Genotypes responded differently to drought stress with the tolerant genotype showing a higher capacity for reactive oxygen species scavenging and maintaining energy supply than the sensitive genotype. The sensitive genotype had a greater number of proteins with significant differential abundance than the tolerant genotypes due to drought. The different patterns in protein abundance induced by drought stress may potentially be utilised to screen and select candidate soybean lines with improved drought tolerance.

scholarly journals Physiological and Comparative Proteomic Analysis Reveals Different Drought Responses in Roots and Leaves of Drought-Tolerant Wild Wheat (Triticum boeoticum)

PLoS ONE ◽  
2015 ◽  
Vol 10 (4) ◽  
pp. e0121852 ◽  
Author(s):  
Hui Liu ◽  
Muhammad Abdul Rab Faisal Sultan ◽  
Xiang li Liu ◽  
Jin Zhang ◽  
Fei Yu ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Wild Wheat ◽  
Comparative Proteomic Analysis ◽  
Drought Tolerant ◽  
Triticum Boeoticum

scholarly journals Pathogenic Variation of Phakopsora pachyrhizi Isolates on Soybean in the United States from 2006 to 2009

Plant Disease ◽  
2012 ◽  
Vol 96 (1) ◽  
pp. 75-81 ◽  
Author(s):  
M. Twizeyimana ◽  
G. L. Hartman
Keyword(s):  
United States ◽  
Resistance Genes ◽  
The United States ◽  
Soybean Rust ◽  
Phakopsora Pachyrhizi ◽  
Soybean Genotype ◽  
Pathogenic Variation ◽  
Differential Set ◽  
Soybean Genotypes ◽  
Soybean Leaves

The introduction of Phakopsora pachyrhizi, the cause of soybean rust, into the United States is a classic case of a pathogen introduction that became established in a new geographical region overwintering on a perennial host (kudzu, Pueraria lobata). The objective of our study was to classify the pathogenic variation of P. pachyrhizi isolates collected in the United States, and to determine the spatial and temporal associations. In total, 72 isolates of P. pachyrhizi collected from infected kudzu and soybean leaves in the United States were purified, then established and increased on detached soybean leaves. These isolates were tested for virulence and aggressiveness on a differential set of soybean genotypes that included six genotypes with known resistance genes (Rpp), one resistant genotype without any known characterized resistance gene, and a susceptible genotype. Three pathotypes were identified among the 72 U.S. P. pachyrhizi isolates based on the virulence of these isolates on the genotypes in the differential set. Six aggressiveness groups were established based on sporulating-uredinia production recorded for each isolate on each soybean genotype. All three pathotypes and all six aggressiveness groups were found in isolates collected from the southern region and from both hosts (kudzu or soybean) in 2008. Shannon's index based on the number of pathotypes indicated that isolates from the South region were more diverse (H = 0.83) compared with the isolates collected in other regions. This study establishes a baseline of pathogenic variation of P. pachyrhizi in the United States that can be further compared with variation reported in other regions of the world and in future studies that monitor P. pachyrhizi virulence in association to deployment of rust resistance genes.

scholarly journals Identification of Two Novel Wheat Drought Tolerance-Related Proteins by Comparative Proteomic Analysis Combined with Virus-Induced Gene Silencing

2018 ◽  
Vol 19 (12) ◽  
pp. 4020 ◽  
Author(s):  
Xinbo Wang ◽  
Yanhua Xu ◽  
Jingjing Li ◽  
Yongzhe Ren ◽  
Zhiqiang Wang ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Gene Silencing ◽  
Proteomic Analysis ◽  
Crop Yields ◽  
Stress Conditions ◽  
Virus Induced Gene Silencing ◽  
Knock Down ◽  
Comparative Proteomic Analysis ◽  
Related Proteins

Drought is a major adversity that limits crop yields. Further exploration of wheat drought tolerance-related genes is critical for the genetic improvement of drought tolerance in this crop. Here, comparative proteomic analysis of two wheat varieties, XN979 and LA379, with contrasting drought tolerance was conducted to screen for drought tolerance-related proteins/genes. Virus-induced gene silencing (VIGS) technology was used to verify the functions of candidate proteins. A total of 335 differentially abundant proteins (DAPs) were exclusively identified in the drought-tolerant variety XN979. Most DAPs were mainly involved in photosynthesis, carbon fixation, glyoxylate and dicarboxylate metabolism, and several other pathways. Two DAPs (W5DYH0 and W5ERN8), dubbed TaDrSR1 and TaDrSR2, respectively, were selected for further functional analysis using VIGS. The relative electrolyte leakage rate and malonaldehyde content increased significantly, while the relative water content and proline content significantly decreased in the TaDrSR1- and TaDrSR2-knock-down plants compared to that in non-knocked-down plants under drought stress conditions. TaDrSR1- and TaDrSR2-knock-down plants exhibited more severe drooping and wilting phenotypes than non-knocked-down plants under drought stress conditions, suggesting that the former were more sensitive to drought stress. These results indicate that TaDrSR1 and TaDrSR2 potentially play vital roles in conferring drought tolerance in common wheat.

scholarly journals Comparative Proteomics of Root Apex and Root Elongation Zones Provides Insights into Molecular Mechanisms for Drought Stress and Recovery Adjustment in Switchgrass

Proteomes ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 3 ◽  
Author(s):  
Zhujia Ye ◽  
Sasikiran Reddy Sangireddy ◽  
Chih-Li Yu ◽  
Dafeng Hui ◽  
Kevin Howe ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Drought Stress ◽  
Molecular Mechanisms ◽  
Root Zone ◽  
Root Apex ◽  
Root Tip ◽  
Leaf Water Content ◽  
Severe Drought ◽  
Proteomics Data ◽  
Drought Treatment

Switchgrass plants were grown in a Sandwich tube system to induce gradual drought stress by withholding watering. After 29 days, the leaf photosynthetic rate decreased significantly, compared to the control plants which were watered regularly. The drought-treated plants recovered to the same leaf water content after three days of re-watering. The root tip (1cm basal fragment, designated as RT1 hereafter) and the elongation/maturation zone (the next upper 1 cm tissue, designated as RT2 hereafter) tissues were collected at the 29th day of drought stress treatment, (named SDT for severe drought treated), after one (D1W) and three days (D3W) of re-watering. The tandem mass tags mass spectrometry-based quantitative proteomics analysis was performed to identify the proteomes, and drought-induced differentially accumulated proteins (DAPs). From RT1 tissues, 6156, 7687, and 7699 proteins were quantified, and 296, 535, and 384 DAPs were identified in the SDT, D1W, and D3W samples, respectively. From RT2 tissues, 7382, 7255, and 6883 proteins were quantified, and 393, 587, and 321 proteins DAPs were identified in the SDT, D1W, and D3W samples. Between RT1 and RT2 tissues, very few DAPs overlapped at SDT, but the number of such proteins increased during the recovery phase. A large number of hydrophilic proteins and stress-responsive proteins were induced during SDT and remained at a higher level during the recovery stages. A large number of DAPs in RT1 tissues maintained the same expression pattern throughout drought treatment and the recovery phases. The DAPs in RT1 tissues were classified in cell proliferation, mitotic cell division, and chromatin modification, and those in RT2 were placed in cell wall remodeling and cell expansion processes. This study provided information pertaining to root zone-specific proteome changes during drought and recover phases, which will allow us to select proteins (genes) as better defined targets for developing drought tolerant plants. The mass spectrometry proteomics data are available via ProteomeXchange with identifier PXD017441.

scholarly journals Integrated transcriptome and small RNA sequencing analyses reveal a drought stress response network in Sophora tonkinensis

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Ying Liang ◽  
Kunhua Wei ◽  
Fan Wei ◽  
Shuangshuang Qin ◽  
Chuanhua Deng ◽  
...  
Keyword(s):  
Drought Stress ◽  
Rna Sequencing ◽  
Small Rna ◽  
Stress Responses ◽  
Molecular Mechanisms ◽  
Differentially Expressed ◽  
Small Rna Sequencing ◽  
Severe Drought ◽  
A Genome ◽  
Sophora Tonkinensis

Abstract Background Sophora tonkinensis Gagnep is a traditional Chinese medical plant that is mainly cultivated in southern China. Drought stress is one of the major abiotic stresses that negatively impacts S. tonkinensis growth. However, the molecular mechanisms governing the responses to drought stress in S. tonkinensis at the transcriptional and posttranscriptional levels are not well understood. Results To identify genes and miRNAs involved in drought stress responses in S. tonkinensis, both mRNA and small RNA sequencing was performed in root samples under control, mild drought, and severe drought conditions. mRNA sequencing revealed 66,476 unigenes, and the differentially expressed unigenes (DEGs) were associated with several key pathways, including phenylpropanoid biosynthesis, sugar metabolism, and quinolizidine alkaloid biosynthesis pathways. A total of 10 and 30 transcription factors (TFs) were identified among the DEGs under mild and severe drought stress, respectively. Moreover, small RNA sequencing revealed a total of 368 miRNAs, including 255 known miRNAs and 113 novel miRNAs. The differentially expressed miRNAs and their target genes were involved in the regulation of plant hormone signal transduction, the spliceosome, and ribosomes. Analysis of the regulatory network involved in the response to drought stress revealed 37 differentially expressed miRNA-mRNA pairs. Conclusion This is the first study to simultaneously profile the expression patterns of mRNAs and miRNAs on a genome-wide scale to elucidate the molecular mechanisms of the drought stress responses of S. tonkinensis. Our results suggest that S. tonkinensis implements diverse mechanisms to modulate its responses to drought stress.

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