Factors affecting the potential contributions of N2 fixation by legumes in Australian pasture systems

2012 ◽  
Vol 63 (9) ◽  
pp. 759 ◽  
Author(s):  
M. B. Peoples ◽  
J. Brockwell ◽  
J. R. Hunt ◽  
A. D. Swan ◽  
L. Watson ◽  
...  
Keyword(s):  
N2 Fixation ◽  
Subterranean Clover ◽  
Pure Stand ◽  
Legume Species ◽  
Factors Affecting ◽  
Net Productivity ◽  
Annual Legumes ◽  
Soil Constraints ◽  
Annual Medics ◽  
Perennial Legumes

The amounts of foliage nitrogen (N) fixed by various annual and perennial legumes growing in Australian pastures range from <10 to >250 kg N/ha.year. Differences in N2 fixation result from variations in the proportion of the legume-N derived from atmospheric N2 (%Ndfa) and/or the amount of legume-N accumulated during growth. On-farm surveys of %Ndfa achieved by legumes growing in farmers’ paddocks in Australia indicated that N2 fixation contributed >65% of the legume’s N requirements in three-quarters of the annual legumes examined, but this decreased to two-thirds of lucerne (Medicago sativa; also known as alfalfa), and half of white clover (Trifolium repens) samples. Factors such as low numbers or the poor effectiveness of rhizobial strains in the soil, water stress, high soil concentrations of N, and nutrient disorders contribute to poor nodulation and %Ndfa values <65%, but there is also evidence that the observed %Ndfa can be dependent on the legume species present, and whether the legume is grown in a pure stand or in a mixed sward. The accumulation of legume-N relates primarily to the legume content and net productivity of the pasture. For many legume species, ~20 kg of shoot-N is fixed on average for every tonne of herbage dry matter produced. Legume productivity can be influenced by (i) sowing and establishment techniques and other strategies that enhance the legume content in pasture swards; (ii) the amelioration of soil constraints; (iii) the use of new legume species (and host–rhizobial strain combinations) that are more tolerant of hostile soil environments than subterranean clover (T. subterraneum) or annual medics (Medicago spp); and (iv) the inclusion of perennials such as lucerne to offset the year-to-year variability in productivity and N2 fixation that is a common occurrence with annual legumes.

Survey of the productivity, composition and estimated inputs of fixed nitrogen by pastures in central-western New South Wales

2004 ◽  
Vol 44 (12) ◽  
pp. 1165 ◽  
Author(s):  
A. M. Bowman ◽  
W. Smith ◽  
M. B. Peoples ◽  
J. Brockwell
Keyword(s):  
White Clover ◽  
N2 Fixation ◽  
Dry Matter ◽  
New South ◽  
Perennial Grass ◽  
New South Wales ◽  
Subterranean Clover ◽  
Legume Species ◽  
Forage Production ◽  
South Wales

Total productivity and legume nitrogen fixation (N2 fixation) in dryland pastures were examined in a 2 year study (1999–2001) on 118 farms in central-western New South Wales. Pasture exclosure cages, placed at 217 on-farm sites, were harvested on 7 occasions and the foliage hand-sorted according to species in order to measure shoot dry matter (DM). The separated legume shoot material collected in spring 1999 (52 different legume samples) and 2000 (76 different legume samples) from a subset of representative pastures (41 cages on 28 different farms in 1999, 32 cages on 25 different farms in 2000) was also analysed for concentration of nitrogen (%N) and 15N natural abundance. These data were subsequently used to calculate the proportion of the legume shoot N derived from atmospheric N (%Ndfa), comparative measures of the relative efficiency of N2 fixation (kg N fixed/t DM accumulated) and the amounts of shoot N fixed (kg N/ha). The survey encompassed 8 common pasture types, and 5 others that were less common, ranging from native perennial grass pastures with little legume content to lucerne (Medicago sativa L.) pastures with and without companion clovers. Fifteen legume species were found in the pastures, some only occasionally. Lucerne and white clover (Trifolium repens L.) were the only perennials. Mean spring estimates of %Ndfa were similar in 1999 and 2000 for lucerne (72 and 81%, respectively), rose clover (T. hirtum All., 82 and 77%) and annual medics (Medicago spp., 89 and 86%). For the remaining 12 legume species, measures of %Ndfa ranged from 64 to 95% and averaged 83%. Shoot %N contents were greater for lucerne than for the other 14 legumes and this was reflected in the comparative measures of N2 fixation which ranged from 14.5 kg N/t DM for rose clover to 25.7 kg N/t DM for lucerne in 2000. The most productive pasture type comprised lucerne plus balansa clover [T. michelianum Savi var. balansae (Boiss.) Azn.], white clover or arrowleaf clover (T. vesiculosum Savi), but all pasture types that contained lucerne were highly productive. Spring was the most productive season and summer the least. Lucerne was overwhelmingly the most productive legume and was responsible for >83% of the fixed N in those pastures that contained both lucerne and other legumes. Lucerne productivity was approximately uniform throughout the year whereas, for other pastures, especially those based on rose clover or subterranean clover (T. subterraneum L.), there were sharp peaks in spring and little or no dry matter production over summer. The presence of lucerne in pastures significantly (P<0.05) reduced broadleaf weeds. It was concluded that, where there are requirements in central-western New South Wales agriculture for uniform forage production throughout the year and a high input of fixed N, lucerne is substantially superior to other species.

Selection of reference plants for 15N natural abundance assessment of N2 fixation by crop and pasture legumes in south-west Australia

1994 ◽  
Vol 45 (1) ◽  
pp. 133 ◽  
Author(s):  
JS Pate ◽  
MJ Unkovich ◽  
EL Armstrong ◽  
P Sanford
Keyword(s):  
N2 Fixation ◽  
Subterranean Clover ◽  
15N Natural Abundance ◽  
Natural Abundance ◽  
Field Pea ◽  
Legume Species ◽  
Reference Species ◽  
Rooting Zone ◽  
South West ◽  
Reference Plants

The 15N natural abundance (S15N) of the shoot total N of a range of non-N2 fixing potential reference species was compared with that of nodulated field pea (Pisum sativum L.), narrow leafed lupin (Lupinus angustijolius L.) or subterranean clover (Trijolium subterraneum L.) across a range of field sites, to which N fertilizers had not been applied in the season of study. Shoot S15N values of reference species lay mostly within the range from +3 to +5%o and there was some evidence of lower S15N values in gramineaceous than dicotyledonous non-legume species. Continuous sampling within crops of each legume showed S15N values to differ consistently between and within potential reference species through the season. The S15N values of seedlings of four non legume species in a lupin crop were closer to that of soil N03-N (S15N = 4.2%o) than soil NH4-N (S15N = 7.9%0). Shoot S15N values of non-nodulated pea, lupin and subterranean clover, and a range of possible reference species all sand-cultured on a defined nitrate source (S15N = 7.5%), suggested little or no discrimination during utilization of nitrate. However, when four candidate reference species were sand cultured with nodulated actively fixing subterranean clover on the same nitrate source, the S15N of the ryegrass was lowered significantly below that of the NO3. Field plot comparisons of nine potential reference species with nodulated field pea showed certain species to resemble field pea more closely than others in terms of the S15N value of their shoots. Reference plants sampled within or well outside the rooting zone of an actively fixing legume (subterranean clover, field pea or lupin) showed significantly lower shoot S15N of mixed grass components when harvested in root contact with, as opposed to well distant from, subterranean clover. A similar effect was observed for barley within v. outside the rooting zone of pea, but no such effects were observed between capeweed and subterranean clover, pea and radish, or for any of five reference plants matched with lupin. The data are utilized to select appropriate reference plants for field assessments of N2 fixation under south-west Australian conditions.

scholarly journals Pasture legume establishment from oversowing in drought-prone hill country

1993 ◽  
pp. 101-104
Author(s):  
M.H. Awan ◽  
P.D. Kemp ◽  
M.A. Choudhary ◽  
D.J. Barker
Keyword(s):  
Plant Density ◽  
Subterranean Clover ◽  
Soil Conditions ◽  
Research Station ◽  
Legume Species ◽  
Hill Country ◽  
Herbage Yield ◽  
Definition Of ◽  
Perennial Legumes ◽  
Pasture Legume

Plant and environmental factors that affect pasture legume establishment from oversowing were examined in a series of experiments at Poukawa Research Station near Hastings. A combination of 2 sowing seasons and 2 soil conditions gave cool/ dry, cool/wet, warm/dry and warm/wet. The rainfall in the trial year (1992) was 933 mm compared with the mean of 771 mm. Eleven species (5 annual and 6 perennial legumes) from Lotus, Medicago and Tr$olium genera were used. Establishment was poor, less than 10% of total herbage yield (6 and 8 months after oversowing for the cool and warm seasons, respectively) being contributed by any of the legume species. The major cause of poor establishment was poor germination. Eighty percent of sown seed failed to produce a seedling. Although germination was worse under cool (8°C) conditions, particularly for subterranean clover, the major cause of poor germination was not identified. The usefulness of new legume species in dryland hill pastures will depend on the species being suited to establishment from oversowing and a better definition of the factors that affect successful oversowing. Keywords: drought, herbage yield, hill country, Lotus, Medicago, moisture, oversowing, pasture establishment, plant density, temperature, Trifolium

Response of subterranean clover, balansa clover, and gland clover to lime when grown in mixtures on an acid soil

2008 ◽  
Vol 59 (9) ◽  
pp. 824 ◽  
Author(s):  
R. C. Hayes ◽  
B. S. Dear ◽  
B. A. Orchard ◽  
M. B. Peoples ◽  
P. L. Eberbach
Keyword(s):  
Seed Yield ◽  
N2 Fixation ◽  
Acid Soil ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Moisture Stress ◽  
Soil Conditions ◽  
Pasture Production ◽  
Mn Toxicity ◽  
Annual Legumes

This study compared the relative tolerances of subterranean clover (Trifolium subterraneum L.), balansa clover (T. michelianum Savi.), and gland clover (T. glanduliferum Boiss.) to acid soil conditions. Seed yield, seedling density, herbage production, N2 fixation, and herbage mineral composition of the 3 legumes were assessed when grown on an acid soil (pHCa of 4.3 and 15% exchangeable Al [0–0.10 m]) with and without the addition of lime (CaCO3). Annual legume species were sown in a mixed sward together with burr medic (Medicago polymorpha L.), and in mixtures with either lucerne (Medicago sativa L.), chicory (Cichorium intybus L.), or phalaris (Phalaris aquatica L.). Due to drier than average seasonal conditions, none of the perennial species persisted beyond the first summer. Lime increased the herbage production of annual legumes by 18–22% and total pasture production by 14% in both 2002 and 2003. Subterranean clover was the most tolerant of the annual legumes to acid soil conditions, showing no visible toxicity symptoms and no response to lime in terms of seed yield. In contrast, both balansa and gland clovers exhibited visual symptoms of manganese toxicity in the absence of lime, with Mn concentrations in the shoots of 817 mg/kg and 626 mg/kg, respectively. Both species responded positively to lime with seed yields increasing by 45% and 124%, respectively. Lime increased the proportion of herbage N derived from N2 fixation by subterranean clover from 29 to 40% and by gland clover from 30 to 43%. Lime had no effect on the proportion of N2 fixed by balansa clover (29–31%), suggesting a suboptimal symbiosis of rhizobia with that species. Adding chicory or phalaris to the pasture mix increased sward herbage production in the establishment year by 39% and 21%, respectively. Based on leaf symptoms and herbage yield responses to lime, Mn toxicity was present in lucerne with tissue levels of up to 916 mg/kg, but no symptoms were observed in chicory (1129 mg/kg) or phalaris (403 mg/kg). Chicory and phalaris were more tolerant of acidity and high levels of Mn than lucerne, gland clover, and balansa clover. The study highlighted the value of the small-seeded annual legumes, balansa clover and gland clover, to the production of mixed pasture swards even in drier than average seasonal conditions. Although more sensitive to acid soils than subterranean clover, they set a greater number of seeds and, in the case of balansa clover, a greater weight of seed under moisture stress in the establishment year than the larger seeded subterranean clover.

Fungal diseases of temperate annual pasture legumes in southern Queensland

1999 ◽  
Vol 39 (6) ◽  
pp. 699 ◽  
Author(s):  
J. M. Mackie ◽  
D. L. Lloyd ◽  
M. J. Ryley ◽  
J. A. G. Irwin
Keyword(s):  
Rhizoctonia Solani ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Fungal Diseases ◽  
Colletotrichum Trifolii ◽  
Annual Legumes ◽  
Pasture Legumes ◽  
Colletotrichum Destructivum ◽  
Annual Medics ◽  
Annual Medicago

Summary. Diseases of temperate annual pasture legumes in subtropical southern Queensland were surveyed during 1992 and 1993. The following pathogenic organisms were recorded: Colletotrichum trifolii, Stemphylium vesicarium, Oidium sp., Uromyces anthyllidis, Uromyces striatus and Pseudopeziza medicaginis from annual Medicago spp.; Rhizoctonia solani and Colletotrichum destructivum from Ornithopus spp.; and Oidium sp. from Trifolium subterraneum. Three of these disease interactions had not been previously recorded in Queensland and 5 were new reports for Australia. Rust was the most frequently observed and widespread disease on annual medics (44% of M. polymorpha samples). All other diseases of annual medics were found infrequently (2–18% of samples). In contrast, both serradella and subterranean clover were relatively free from any diseases. The years during which the survey was conducted were dry (as low as 31% of mean March–October rainfall) and the expression of disease may have been restricted. Nevertheless, this improved knowledge of diseases of temperate annual legumes in southern Queensland will assist in the future selection and breeding of suitable cultivars for use in the subtropics.

scholarly journals Australian experience with cool season annual legumes - the challenge to develop environmentally sustainable farming systems

2003 ◽  
Vol 11 ◽  
pp. 51-59
Author(s):  
B.S. Dear
Keyword(s):  
Low Cost ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Farming Systems ◽  
Environmentally Sustainable ◽  
Change Of Direction ◽  
Annual Legumes ◽  
Native Pastures ◽  
Annual Medics ◽  
Australian Experience

Australian farming systems have traditionally relied on annual legumes such as subterranean clover (Trifolium subterraneum) and annual medics (Medicago spp.) in either short-term pastures in rotation with crops or permanent pastures to provide low cost biologically fixed N and a high quality forage for livestock. The role of legumes in farming systems is now being reassessed because of the recognition that their extensive use is associated with widespread soil acidification, loss of species diversity in native pastures and increasing dryland salinity. In the future, annual legumes are more likely to be sown in mixtures with deep-rooted perennial pasture species, both in permanent pastures and in rotation with crops, to improve hydrological balance in the landscape. As a result, there is a change of direction in annual legume selection and breeding programs within Australia with a greater focus on the ability of legumes to coexist with perennial species, as well as on characteristics such as an extended growing season and deeper rooting habit to exploit subsoil water. There is also a trend towards increasing the diversity of annual legume species sown in pasture mixes to better exploit paddock variation and variable seasonal conditions.

Evaluation of pasture legumes sown into a prepared seedbed at Tamworth, New South Wales. 2. Seed production and seed reserves

1993 ◽  
Vol 33 (3) ◽  
pp. 299 ◽  
Author(s):  
GM Lodge
Keyword(s):  
Seed Production ◽  
New South ◽  
New South Wales ◽  
Pasture Legumes ◽  
South Wales ◽  
Medicago Scutellata ◽  
Seed Reserves ◽  
Annual Medics ◽  
Perennial Legumes ◽  
Seed Yields

Plots sown in 1983 were used to examine the seed production and reserves (residual hardseeds) of 15 annual legumes over 5 years at Tamworth in northern New South Wales. Seed production characteristics were measured in 1983 for these annuals, and for 6 perennial legumes. After the annuals had set seed in 1983, an area of the plots was sprayed to prevent flowering in subsequent years, and the rates of decline in seed reserves were compared with those from areas that seeded annually. Seed yields of the perennials were often significantly lower than those of the annuals except Trifolium glomeratum. Seed yields of T. subterraneum var. subterraneum cvv. Seaton Park and Woogenellup were significantly higher than those of cv. Nungarin and T. subterrarzeum var. brachycalycinum cv. Clare. Seed numbers were lowest for Medicago scutellata cv. Sava among the annual medics, and for Nungarin among subterranean clovers. There was little relationship between the mean number of seeds produced from 1983-86 and maturity grading, and between seed numbers and relative dry matter yield. Seed reserves decreased over 5 years by more than 90% in the sprayed treatments of all species. This decrease was not continuous, with the largest declines occurring from December 1983 to August 1984. In the sprayed treatments of Seaton Park, Woogenellup, and Clare, and in both treatments of Astragalus hamosus cv. Ioman, Vicia dasycarpa var. villosa cv. Namoi, and T. hirtum cv. Hykon, none of the original 1983 seed was recovered in 1987. For the annual medics and Nungarin, the number of residual hardseeds in the sprayed treatments in 1987 was about 3-5% of the seed produced in 1983. From 1983 to 1987, seed numbers in the unsprayed treatments declined by 7040% for the annual medics and by 85-95% for Seaton Park, Woogenellup, and Clare. Long-term persistence of annual medics and Nungarin depended on seed production in most years and the maintenance of a high number of residual hardseeds in the soil. In contrast, the mid- and late-maturing subterranean clovers Woogenellup and Clare had low seed reserves and were dependent on seed production in most years for their continued regeneration.

The transference of nitrogen from pasture legumes to an associated grass under several systems of management in pot culture

1965 ◽  
Vol 16 (6) ◽  
pp. 915 ◽  
Author(s):  
JR Simpson
Keyword(s):  
White Clover ◽  
Subterranean Clover ◽  
Experimental Period ◽  
The Other ◽  
Temporary Increase ◽  
Winter Period ◽  
Pasture Legumes ◽  
Perennial Legumes ◽  
Intact Root ◽  
Pot Culture

The extent of underground transference of nitrogen from three pasture legumes, each growing in association with a grass, has been studied in pot culture under several systems of management during the first 12–18 months after sowing. The three legumes performed quite differently. Subterranean clover did not release any nitrogen until senescence and then produced a rapid transference. White clover was competitive for nitrogen until the autumn-winter period. Lucerne released nitrogen gradually over the whole experimental period. Frequent defoliation of the legumes reduced competition for nitrogen by white clover but also reduced transference from the other legumes. Killing the perennial legumes produced only a small temporary increase in transference. Wilting and temporary drying treatments also reduced the transference. Thus there was no evidence that the nitrogen transference from lucerne was due to a shedding or decay of nodules induced by defoliation; it could equally well be due to direct excretion of nitrogen from the intact root system. The significance of the results in pasture establishment in infertile areas is discussed.

Flower initiation in pasture legumes. I. Factors affecting flower initiation in Trifolium subterraneum L.

1955 ◽  
Vol 6 (2) ◽  
pp. 211 ◽  
Author(s):  
Y Aitken
Keyword(s):  
Low Temperature ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Cold Period ◽  
Effect Of Temperature ◽  
Flower Initiation ◽  
Temperature Level ◽  
Factors Affecting ◽  
Pasture Legumes ◽  
Precise Knowledge

The value of the annual legume Trifolium subterraneum L. (subterranean clover) in Australian agriculture warrants more precise knowledge of factors affecting flowering and prolific seeding. The effect of temperature and photoperiod on flower initiation in early and later flowering varieties has been investigated in an effort to determine the geographical limits of the use of subterranean clover in Australia. At any time of sowing, the length of the growing season of a variety depends greatly on the variety's response to the temperature level and to the photoperiod of the first few weeks after germination. In all varieties of subterranean clover so far examined flower initiation is accelerated by a period of low temperature. In the later varieties, flower initiation is prevented by an insufficient period of low temperature. The length of the necessary cold period is shortened under longer photoperiod. Early varieties are early flowering because they do not require so long a cold period or so low a temperature as late varieties.

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