scholarly journals The influence of ethanol as a solvent on the gibberellic acid-induced germination of Brachyscome and Allittia (Asteraceae) seeds

2018 ◽  
Vol 66 (2) ◽  
pp. 101
Author(s):  
Rina Aleman ◽  
Manfred Jusaitis ◽  
Joan Gibbs ◽  
Phil Ainsley ◽  
Fleur Tiver

Gibberellic acid (GA3) is routinely used as a germination stimulant for seeds. However, the methods used to dissolve GA3 powder – particularly if using organic solvents – have the potential to affect germination outcomes. In this study we examined the influence of the solvent ethanol, used to dissolve GA3, on the seed germination of 14 species of Brachyscome and two species of Allittia. These species are important Australian native composites with potential for use in habitat restoration. Seeds of 11 of these species were found to be particularly responsive to GA3. However, the use of a low concentration of ethanol (0.5%) to dissolve GA3 affected subsequent germination outcomes, with four species responding positively and eight negatively to this solvent. The pure effect of GA3 was therefore masked by the presence of small concentrations of ethanol, whose effects varied between the species examined. Because of these potentially confounding effects of ethanol, we recommend the use of pure water for dissolution of GA3 when testing seed germination responses in these genera.

2009 ◽  
Vol 37 (1) ◽  
pp. 33-41 ◽  
Author(s):  
B. Pascual ◽  
A. San Bautista ◽  
N. Pascual Seva ◽  
R. García Molina ◽  
S. López-Galarza ◽  
...  

2007 ◽  
Vol 59 (3) ◽  
pp. 227-231 ◽  
Author(s):  
S. Zivkovic ◽  
M. Devic ◽  
B. Filipovic ◽  
Z. Giba ◽  
D. Grubisic

The influence of high NaCl concentrations on seed germination in both light and darkness was examined in the species Centaurium pulchellum, C. erythraea, C. littorale, C. spicatum, and C. tenuiflorum. Salt tolerance was found to depend on the life history of the seeds. To be specific, seeds of all five species failed to complete germination when exposed to continuous white light if kept all the time in the presence of 100-200 mM and greater NaCl concentrations. However, when after two weeks NaCl was rinsed from the seeds and the seeds were left in distilled water under white light for an additional two weeks, all species completed germination to a certain extent. The percent of germination not only depended on NaCl concentration in the prior medium, but was also species specific. Thus, seeds of C. pulchellum, C. erythraea, and C. littorale completed germination well almost irrespective of the salt concentration previously experienced. On the other hand, seeds of C. tenuiflorum completed germination poorly if NaCl concentrations in the prior media were greater than 200 mM. When seeds after washing were transferred to darkness for an additional 14 days, they failed to complete germination if previously imbibed on media containing NaCl concentrations greater than 400 mM. However, the seeds of all species, even if previously imbibed at 800 mM NaCl, could be induced to complete germination in darkness by 1 mM gibberellic acid. .


2016 ◽  
Vol 3 (1) ◽  
pp. 27-37
Author(s):  
Zaynab Taghinezad ◽  
Massoud Dehdari ◽  
Amin Mirshekari ◽  
Hossain Zainali ◽  
◽  
...  

1977 ◽  
Vol 4 (6) ◽  
pp. 849 ◽  
Author(s):  
Y Esashi ◽  
Y Ohhara

Non-dormant, upper cocklebur (Xanthium pensylvanicum Wallr.) seeds, incapable of germinating under ordinary conditions, can germinate when previously subjected to anaerobiosis; this has been termed the anaerobic induction of seed germination. Aerobic presoaking of the seeds was also required for successful anaerobic induction, and exerted two counter-acting effects on seed germination. When the time period of aerobic presoaking was sufficiently prolonged, the increasing duration of an anaerobic treatment resulted in proportional increase of germination potential but, when it was short, the effect of the anaerobiosis was saturated in a few days. Prolonging the aerobic presoaking period caused less response of the seed to the anaerobic induction, suggesting the development of some germination-inhibiting system during the aerobic presoaking period. This system could not develop in the absence of O2 or at low temperature. Thus, low temperature during prolonged presoaking produced a maximal response to anaerobic induction. Various germination stimulants, CO2, ethylene, gibberellic acid and benzyl adenine, did not significantly alter the effects of the presoaking.


1976 ◽  
Vol 7 (36) ◽  
pp. no-no
Author(s):  
J. B. HEATHER ◽  
R. S. D. MITTAL ◽  
CHARLES J. SIH

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