Reproductive biology and intergeneric breeding compatibility of ornamental Portulaca and Calandrinia (Portulacaceae)

2009 ◽  
Vol 57 (8) ◽  
pp. 697 ◽  
Author(s):  
Priyanka Wickramasinghe ◽  
Dion K. Harrison ◽  
Margaret E. Johnston
Keyword(s):  
Pollen Tube ◽  
Reproductive Biology ◽  
Chromosome Numbers ◽  
Pollen Tube Growth ◽  
Reciprocal Crosses ◽  
Ornamental Horticulture ◽  
Portulaca Grandiflora ◽  
Intergeneric Hybridisation ◽  
Pot Plants ◽  
Novel Products

Portulaca grandiflora Hook and P. umbraticola Kunth (Portulacaceae) are popular garden annuals, and have been bred for improved ornamental value. However, limited research has been published on hybridisation of Portulaca, with no reports on intergeneric hybridisation. Calandrinia balonensis Lindley and Calandrinia sp. nov. (not yet fully classified) are floriferous Australian Portulacaceae species, with potential as novel flowering pot plants, and are potential candidates for breeding with ornamental Portulaca. We studied the reproductive biology of these four species and breeding compatibility for reciprocal crosses of P. grandiflora × C. balonensis (2n = 18) and P. umbraticola × C. sp. nov. (2n = 24). All four species produced seeds for intraspecific outcrosses. P. grandiflora and C. sp. nov. are partially self-compatible whereas P. umbraticola and C. balonensis are highly self-incompatible. Autogamy was detected only for P. grandiflora. Reciprocal crosses of P. grandiflora × C. balonensis and P. umbraticola × C. sp. nov. with similar chromosome numbers did not produce seeds, primarily because of pollen–pistil incompatibility that prevents pollen-tube growth within the stigmata. Methods to overcome hybridisation barriers of these species combinations need to be established to create novel products for ornamental horticulture.

Onset and duration of stigma receptivity in Kunzea pomifera (Myrtaceae)

2006 ◽  
Vol 54 (6) ◽  
pp. 559 ◽  
Author(s):  
T. Page ◽  
G. M. Moore ◽  
J. Will ◽  
G. M. Halloran
Keyword(s):  
Pollen Tube ◽  
Reproductive Biology ◽  
Peroxidase Activity ◽  
Pollen Tube Growth ◽  
Seed Set ◽  
Stigma Receptivity ◽  
Controlled Pollination ◽  
Germinated Pollen ◽  
New Crop ◽  
Days After Anthesis

The onset and duration of stigma receptivity in K. pomifera was evaluated by observing stigma peroxidase activity, pollen-tube growth and seed set, following controlled pollination of flowers of different ages. Peroxidase activity was negligible from 1 day before to 4 days after anthesis, increasing to a peak of 65% at Day 13. The percentage of pistils bearing germinated pollen and ovaries exhibiting pollen-tube entry increased when pollen was applied to the stigma from the day before anthesis, to a maximum at Days 6 and 7 after anthesis, respectively, followed by a decline by Day 9. Under greenhouse conditions the optimum pollination period, measured as the level of set seed after pollination, ranged from 2 to 11 days after anthesis. The knowledge of this aspect of the species reproductive biology is of considerable value in controlled pollinations for its improvement as a new crop.

COMPARATIVE GROWTH AND FUNCTION OF NORMAL AND DEFICIENT POLLEN IN A SUBSTITUTION MONOSOMIC OF CHROMOSOME 3D IN WHEAT

1964 ◽  
Vol 42 (10) ◽  
pp. 1329-1333
Author(s):  
J. G. Bhowal
Keyword(s):  
Pollen Tube ◽  
Competitive Advantage ◽  
Pollen Tube Growth ◽  
Initial Rate ◽  
Tube Growth ◽  
Reciprocal Crosses ◽  
Normal Pollen ◽  
Golden Ball ◽  
And Function ◽  
Monosomic Line

Normal pollen with chromosome 3D from Red Bobs had little competitive advantage over pollen deficient for chromosome 3D when a substituted monosomic line of wheat derived from the hybrid Rescue × Golden Ball was selfed. Although the initial rate of pollen tube growth of the 21-chromosome pollen was greater than that of the 20-chromosome pollen, ultimately the two kinds of pollen functioned in the proportions in which they were formed. The 21-chromosome pollen of the substitution monosomic had an advantage over 20-chromosome pollen in a cross with monosomic 3D of Red Bobs, but not with three other 3D monosomic varieties. However, normal pollen in all the reciprocal crosses showed a competitive advantage, affecting fertilization in up to approximately 90% of the cases. Thus chromosome 3D in the Rescue – Golden Ball medium gives the normal pollen little competitive advantage.

scholarly journals Reproductive biology in cassava: stigma receptivity and pollen tube growth

2019 ◽  
Vol 12 (1) ◽  
pp. 96-111 ◽  
Author(s):  
L.N. Ramos Abril ◽  
L.M. Pineda ◽  
I. Wasek ◽  
M. Wedzony ◽  
H. Ceballos
Keyword(s):  
Pollen Tube ◽  
Reproductive Biology ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Stigma Receptivity

scholarly journals Reproductive biology of Echinodorus grandiflorus (Alismataceae): evidence of self-sterility in populations of the state of São Paulo

Rodriguésia ◽  
2011 ◽  
Vol 62 (1) ◽  
pp. 213-221
Author(s):  
Emerson R. Pansarin ◽  
Ludmila M. Pansarin
Keyword(s):  
Pollen Tube ◽  
Reproductive Biology ◽  
Pollen Tube Growth ◽  
Pollen Viability ◽  
Sao Paulo ◽  
The State ◽  
Tube Growth ◽  
São Paulo ◽  
Natural Conditions ◽  
Effective Pollinators

Abstract The reproductive biology and the pollination of Echinodorus grandiflorus (Cham. & Schltdl.) Micheli were studied in populations native to interior of the State of São Paulo, Brazil. This species blossoms in summer and its flowers offer both nectar and pollen as rewards to pollinators. Nectar is produced in nectaries located at the base of the marginal carpels, opposite the petals. However, the effective pollinators (social and solitary bees), were recorded collecting pollen only. Visits, which can last from one or a few seconds to more than one minute, occur during the whole flower lifespan. Each flower opens at about 5:30 a.m. and lasts circa eight hours. All plants in the studied populations produce only hermaphrodite flowers. Percentage of pollen viability is 75%. The studied populations are self-incompatible and, as a consequence, pollinators are needed to transfer pollen among individuals. In natural conditions and after hand cross-pollinations, all receptacles presented mature achenes. Based on the analyses of pollen tube growth from hand self-pollinated flowers, and as a consequence of achenes abortion circa 30 days after self-pollinations, the populations of E. grandiflorus studied apparently presents a mechanism of late-acting self-sterility.

scholarly journals Proline-rich Extensin-like Receptor Kinases PERK5 and PERK12 are involved in Pollen Tube Growth

2021 ◽  
Author(s):  
Cecilia Borassi ◽  
Ana R. Sede ◽  
Martin A. Mecchia ◽  
Silvina Mangano ◽  
Eliana Marzol ◽  
...  
Keyword(s):  
Cell Wall ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Root Hairs ◽  
Pollen Tubes ◽  
Tube Growth ◽  
Loss Of Function ◽  
Reciprocal Crosses ◽  
Cell Wall Assembly ◽  
Production Of Hydrogen

AbstractBackgroundCell wall integrity plays an essential role during polarized cell growth typical of pollen tubes and root hairs. Proline-rich Extensin-like Receptor Kinases (PERK) belong to the hydroxyproline-rich glycoprotein (HRGP) superfamily of cell surface glycoproteins.ResultsHere, we identified two PERKs from Arabidopsis thaliana, PERK5 and PERK12 highly expressed in mature pollen. Pollen tube growth was impaired in the single and double perk5-1 perk12-1 loss of function mutants, with a moderate impact on seed production. When the segregation of self- and reciprocal-crosses of the perk5-1, perk5-2 and perk12-1 single mutants, and reciprocal-crosses of the perk5-1 perk12-1 double mutant were carried out, a male gametophytic defect was found, indicating that perk5-1 and perk12-1 mutants carry defective pollen tubes, resulting in deficient pollen transmission. Furthermore, double perk5-1 perk12-1 mutants show excessive accumulation of pectins and cellulose at the cell wall pollen of the tube tip. In addition, an upregulation of cytoplasmic ROS levels were detected by using 2,7-dichlorofluorescein diacetate probe (H2DCF-DA), and in agreement, similar results were obtained with HyPer, a genetically encoded YFP-based radiometric sensor, which is used to follow the production of hydrogen peroxide (H2O2). Single and double perk5-1 perk12-1 mutants show higher levels of cytoplasmic H2O2 in their pollen tube tips.ConclusionsTaken together, our results suggest that PERK5 and PERK12 are necessary for proper pollen tube growth highlighting their role on cell wall assembly and ROS homeostasis.

Inhibition of Pollen Tube Growth by Diffusate and Extract of Grape Pistils

1989 ◽  
Vol 58 (3) ◽  
pp. 515-521 ◽  
Author(s):  
Goro OKAMOTO ◽  
Ikuo SHIBUYA ◽  
Miwa FURUICHI ◽  
Kazuo SHIMAMURA
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Tube Growth
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