Content of acid and alkaline phosphatases in lymphocytes of peripheral blood and hematopoietic organs of intact rats and mice

1978 ◽  
Vol 85 (2) ◽  
pp. 150-151
Author(s):  
E. D. Gol'dberg ◽  
G. V. Karpova ◽  
E. V. Melik-Gaikazyan ◽  
G. N. Pakhryaeva
Keyword(s):  
Peripheral Blood ◽  
Alkaline Phosphatases ◽  
Acid And Alkaline Phosphatases ◽  
Hematopoietic Organs ◽  
Rats And Mice ◽  
Intact Rats

Progestation

1967 ◽  
Vol 56 (3_Suppla) ◽  
pp. S7-S45 ◽  
Keyword(s):  
Tritiated Thymidine ◽  
Succinic Dehydrogenase ◽  
Glandular Epithelium ◽  
Synthetic Activity ◽  
Cell Renewal ◽  
Pulse Labelling ◽  
Uterine Receptivity ◽  
Alkaline Phosphatases ◽  
Monocytic Cells ◽  
Acid And Alkaline Phosphatases

ABSTRACT Autoradiographic, enzymic and histologic studies on uteri of pregnant rats were carried out to follow the endometrial modifications which take place during progestation (days L0 – L4) and culminate in the state of uterine receptivity essential for ovum-implantation. Pulse labelling with tritiated thymidine (radioactive DNA precursor) on L0, L1 and L2 revealed a sequence of cell renewal in luminal and glandular epithelium and endometrial stroma. On L3 and L4 stromal cells showed extensive incorporation of tritiated thymidine. This synthetic activity was associated with endometrial preparation for decidualization and was evoked at least in part, by the surge of oestrogen on L3. All layers of the uterine wall were heavily infiltrated on L0 and resembled the site of an acute inflammatory reaction. Subsequently, polymorphonuclear infiltration diminished and monocytic cells predominated. On L3 a spatial arrangement was observed: eosinophiles were concentrated in the basal endometrium and monocytic cells in the subepithelial stroma. A comparison was made between such a shift in migratory cells in the uterus and similar phenomena which occur in inflammatory and immune reactions. Activities of acid and alkaline phosphatases, of ATP-ase and succinic dehydrogenase were low on L0 and L1 during the periods of infiltration, degeneration and regeneration of luminal and glandular epithelium. Enzymic activities increased on the following days, (L3 and L4). Vascular dilation and engorgement and endometrial oedema were observed near the blastocysts on L4. Most blastocysts incorporated tritiated thymidine after 14.00 h on L4, but some showed uptake before loss of the zona which occurs usually between 14.00 and 16.00 h; therefore, it was assumed that the permeability of the zona increases prior to being shed. Activities of succinic dehydrogenase and acid and alkaline phosphatases were demonstrable in blastocysts on L4 while they were still »free« in the uterine lumen.

Effects of Kanwa on Rat Gastrointestinal Phosphatases

2010 ◽  
Vol 3 (3) ◽  
pp. 1147-1152
Author(s):  
Jacob Bamaiyi ◽  
Omajali ◽  
Sanni Momoh
Keyword(s):  
Alkaline Phosphatase ◽  
Small Intestine ◽  
Acid Phosphatase ◽  
Sodium Carbonate ◽  
Elevated Level ◽  
Food Additive ◽  
Alkaline Phosphatases ◽  
Acid And Alkaline Phosphatases ◽  
Diagnostic Indices ◽  
High Level

This study investigates the effects of kanwa on rat gastrointestinal phosphatases. The rats were administered 7% w/v concentration of  trona (Kanwa) orally for a period of two weeks in order to investigate how this compound is being used as food additive in some homes in Nigeria. The Kanwa used in this study was the handpicked variety obtained from sellers from Anyigba market in eastern part of Kogi State, Nigeria. Kanwa, a hydrated sodium carbonate (Na2CO3NaHCO3.2H2O) was obtained as a dried lake salt. Acid phosphatase has the ability to dephosphorylate molecules containing phosphate group. The decreased and elevated level in serum or plasma acid and alkaline phosphatases serves as diagnostic indices for various diseases. Results showed that there was increase and decrease of acid phosphatase (ACP) activities in both the stomach and small intestine. The activities of alkaline phosphatase (ALP) fluctuated in the small intestine. However, in the stomach, an increase activity of ALP was noticed throughout the period of ‘Kanwa’ administration. We concluded that although the level of ‘Kanwa’ consumed in most homes may not be toxic if not taken continuously or repeatedly. Thus, continuous consumption should be discouraged as accumulation of high level of ‘Kanwa’ may cause damages or injuries to the various organs/tissues and may disrupt normal body function.

scholarly journals Effect of heating oil on the activity of soil enzymes and the yield of yellow lupine

2011 ◽  
Vol 52 (No. 5) ◽  
pp. 220-226 ◽  
Author(s):  
J. Kucharski ◽  
E. Jastrzębska
Keyword(s):  
Alkaline Phosphatase ◽  
Soil Enzymes ◽  
Urease Activity ◽  
Alkaline Phosphatases ◽  
Acid And Alkaline Phosphatases ◽  
Heating Oil ◽  
Average Activity ◽  
Yellow Lupine ◽  
Experimental Soil ◽  
Limed Soil

The aim of the study was to determine the response of soil enzymes such as dehydrogenases, urease and acid and alkaline phosphatases to heating oil contaminating (0.0, 0.25, 0.5, 0.75, 1.0, 1.5% of soil) the experimental soil supplemented with lime and used for cultivation of yellow lupine of the Markiz variety. An increasing contamination of soil with heating oil stimulated the activity of dehydrogenases and acid and alkaline phosphatases but had a toxic effect on yellow lupine. Lime supplements did not have a significant effect on an average activity of soil dehydrogenases. However, such soil treatment had a significant effect on urease. Increasing heating oil doses in lime-supplemented soil stimulated urease activity, whereas in lime-free soil urease activity was inhibited. The activity of acid and alkaline phosphatase was lower in limed soil than in lime-free soil. The activity of dehydrogenases, urease and alkaline phosphatase in the soil with lupine cultivation was significantly higher than in the unsown soil.

ACID AND ALKALINE PHOSPHATASES IN NORMAL AND DISEASED HUMAN MUSCLE

1958 ◽  
Vol 35 (4) ◽  
pp. 326-343 ◽  
Author(s):  
Evelyn B. Beckett ◽  
G.H. Bourne
Keyword(s):  
Human Muscle ◽  
Alkaline Phosphatases ◽  
Acid And Alkaline Phosphatases

The blood picture resulting from Nuttallia (= Babesia) rodhaini and Nuttallia (= Babesia) microti infections in rats and mice

Parasitology ◽  
1969 ◽  
Vol 59 (4) ◽  
pp. 991-1004 ◽  
Author(s):  
Frank Nowell
Keyword(s):  
Peripheral Blood ◽  
Prepatent Period ◽  
Babesia Microti ◽  
Blood Picture ◽  
Host Animal ◽  
Inoculum Dose ◽  
The University ◽  
Rats And Mice ◽  
Patent Infection

The parasitaemia and associated blood picture following infections of Nuttallia rodhaini in rats and mice, and N. microti in rats are described. After inoculation of the blood stages of these parasites, it is evident that only further blood stages occur in the host animal. Infections with both piroplasms follow a similar course, there being a prepatent period followed by a patent infection which reaches a peak and then disappears. The prepatent period is an artifact due to the rarity of the parasites in the peripheral blood. The patent infection of N. rodhaini in rats and mice rapidly reaches a peak of up to 90% R.B.C. infected. That of N. microti develops more slowly and rarely reaches 20%. Rats show age resistance to both piroplasmoses but none is shown by mice aged up to 1 year against N. rodhaini infection. Increase of the parasite inoculum dose decreases the prepatent period and also the time from infection to the peak. In rats there is a corresponding increase of the peak but in mice this remains constant. N. microti infections in rats are enhanced by splenectomy, the peak being higher but the time from infection to the peak not altering. The parasitaemia produced by both piroplasms results in a temporary anaemia and reticulocytosis. The anaemia is not prolonged which does not suggest autoimmune reactions. Both piroplasms preferentially invade reticulocytes as compared with mature erythrocytes although this is apparently reversed at the onset of reticulocytosis.An explanation of the mechanism of the parasitaemia of the piroplasms in rats and mice is suggested.This paper is a report of work carried out in part for a thesis submitted for the degree of Ph.D. in the University of London. The work was aided by an S.R.C. research studentship. I would like to thank Dr F. E. G. Cox for his supervision and also for performing the splenectomies.

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