scholarly journals Studies on Metatherian Sex Chromosomes VII. Glucose-6-phosphate Dehydrogenase Expression in Tissues and Cultured Fibroblasts of Kangaroos

1978 ◽  
Vol 31 (4) ◽  
pp. 415 ◽  
Author(s):  
PG Johnston ◽  
GB Sharman ◽  
Elizabeth A James ◽  
DW Cooper
Keyword(s):  
Sex Chromosomes ◽  
Cultured Fibroblasts ◽  
Glucose 6 Phosphate Dehydrogenase

Expression of the sex-linked enzyme glucose-6-phosphate dehydrogenase (G6PD) was examined electrophoretically in tissues and cultured fibroblasts of female kangaroo heterozygotes ranging in age from 26 days post part urn to adult. All tissues expressed only the maternally derived allele irrespective of which allele was maternal or paternal in origin.

scholarly journals Studies on Metatherian Sex Chromosomes VIII. Evidence for an Absence of Dosage Compensation at the Glucose-6-phosphate Dehydrogenase Locus in Cultured Cells of Macropus rufogriseus

1978 ◽  
Vol 31 (4) ◽  
pp. 425 ◽  
Author(s):  
Kathie A Raphael ◽  
DW Cooper
Keyword(s):  
Dosage Compensation ◽  
Sex Chromosomes ◽  
Cultured Cells ◽  
Cultured Fibroblasts ◽  
Glucose 6 Phosphate Dehydrogenase

The level of the sex-linked enzyme glucose-6-phosphate dehydrogenase (G6PD) has been measured in cultured fibroblasts of M. rufogriseus and several other kangaroo species. The data obtained support the conclusion obtained from other evidence that there is a failure of dosage compensation at this locus in fibroblasts of females.

scholarly journals Studies on Metatherian Sex Chromosomes. IX. Sex Chromosomes of the Greater Glider (Marsupialia : Petauridae)

1979 ◽  
Vol 32 (3) ◽  
pp. 375 ◽  
Author(s):  
JD Murray ◽  
GM McKay ◽  
GB Sharman
Keyword(s):  
X Chromosome ◽  
Sex Chromosomes ◽  
National Park ◽  
Secondary Constriction ◽  
X Chromosomes ◽  
Cultured Fibroblasts ◽  
Multiple Sex Chromosomes ◽  
Eastern Australia ◽  
Xy Bivalent ◽  
Secondary Constrictions

The greater glider, currently but incorrectly known as Schoinobates vo/ans, is widely distributed in forested regions in eastern Australia. All animals studied from six different localities had 20 autosomes but there were four chromosomally distinct populations. At Royal National Park, N.S.W., all female greater gliders studied had 22 chromosomes including two large submetacentric X chromosomes with subterminal secondary constrictions in their longer arms. This form of X chromosome occurred also at Bondo State Forest, Myall Lakes and Coff's Harbour, N.S.W., and at Eidsvold, Qld. At Coomooboolaroo, Qld, the X chromosome was also a large submetacentric but a secondary constriction occurred in the shorter arm. Two chromosomally distinct types apparently occur in Royal National Park, one with XY m,ales as in all other populations, and one with XY1Y2 males. Y or Yb but not Y 2, chromosomes were eliminated from the bone marrow in all populations but were present in spermatogonia, primary sperrnatocytes and cultured fibroblasts. Animals from Bondo State Forest had three or more acrocentric or metacentric supernumerary chromosomes. [Other keywords: C-banding, eytotaxonomy, multiple sex chromosomes, XY bivalent.]

scholarly journals Single Nucleotide Primer Extension (SNuPE) analysis of the G6PD gene in somatic cells and oocytes of a kangaroo (Macropus robustus)

2000 ◽  
Vol 75 (3) ◽  
pp. 269-274 ◽  
Author(s):  
DEBBIE WATSON ◽  
ANITA S. JACOMBS ◽  
DAVID A. LOEBEL ◽  
EDWARD S. ROBINSON ◽  
PETER G. JOHNSTON
Keyword(s):  
Somatic Cells ◽  
Primer Extension ◽  
Paternal Allele ◽  
Transcriptional Level ◽  
Specific Expression ◽  
Single Nucleotide ◽  
Cultured Fibroblasts ◽  
Preferential Expression ◽  
Single Nucleotide Primer Extension ◽  
Glucose 6 Phosphate Dehydrogenase

cDNA sequence analysis of the X-linked glucose-6-phosphate dehydrogenase (G6PD) gene has shown a base difference between two subspecies of the kangaroo, Macropus robustus robustus (wallaroo) and M. r. erubescens (euro). A thymine residue in the wallaroo at position 358 in exon 5 has been replaced by a cytosine residue in the euro, which accounts for the previously reported electrophoretic difference between the two subspecies. This base difference allowed use of the Single Nucleotide Primer Extension (SNuPE) technique to study allele-specific expression of G6PD at the transcriptional level. We began by examining G6PD expression in somatic cells and observed complete paternal X inactivation in all somatic tissues of adult female heterozygotes, whereas we found partial paternal allele activity in cultured fibroblasts, thus confirming previous allozyme electrophoresis studies. In late dictyate oocytes from an adult heterozygote, the assay also detected expression of both the maternal and paternal alleles at the G6PD locus, with the maternal allele showing preferential expression. Thus reactivation of the inactive paternally derived X chromosome occurs during oogenesis in M. robustus, although the exact timing of reactivation remains to be determined.

scholarly journals Studies on Metatherian Sex Chromosomes I. Inheritance and Inactivation of Sex-linked Allelic Genes determining Glucose-6-phosphate Dehydrogenase Variation in Kangaroos

1975 ◽  
Vol 28 (6) ◽  
pp. 567 ◽  
Author(s):  
PG Johnston ◽  
GB Sharman
Keyword(s):  
Sex Chromosomes ◽  
Backcross Progeny ◽  
Parental Origin ◽  
X Chromosomes ◽  
Parental Phenotype ◽  
The Cross ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Electrophoretic Phenotype

Wallaroos (Macropus robustus robustus), which have the G6PD-F electrophoretic phenotype, crossed with euros (M. r. erubescens), ofG6PD-S phenotype, produced F 1 animals which had only the maternal G6PD type regardless of the direction of the cross. When F 1 hybrids were backcrossed to wallaroos or euros, backcross progeny of either parental phenotype resulted. Sex-linked inheritance of allelic G6PD genes is shown to occur in wallaroos, euros and red kangaroos (M. rufus). Dose compensation for X chromosomes at the G6PD locus in kangaroos is achieved by inactivation of the allele of male parental origin.

Intralysosomal Accumulation of Colloidal Gold-Low Density Lipoprotein Conjugates in Chloroquine-Treated Fibroblasts

1985 ◽  
Vol 43 ◽  
pp. 546-547 ◽  
Author(s):  
Dean A. Handley ◽  
Cynthia M. Arbeeny ◽  
Larry D. Witte
Keyword(s):  
Colloidal Gold ◽  
Cultured Cells ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Coated Vesicle ◽  
Low Density ◽  
Cholesterol Esters ◽  
Cultured Fibroblasts ◽  
Surface Receptors ◽  
Ldl Particles

Low density lipoproteins (LDL) are the major cholesterol carrying particles in the blood. Using cultured cells, it has been shown that LDL particles interact with specific surface receptors and are internalized via a coated pit-coated vesicle pathway for lysosomal catabolism. This (Pathway has been visualized using LDL labeled to ferritin or colloidal gold. It is now recognized that certain lysomotropic agents, such as chloroquine, inhibit lysosomal enzymes that degrade protein and cholesterol esters. By interrupting cholesterol ester hydrolysis, chloroquine treatment results in lysosomal accumulation of cholesterol esters from internalized LDL. Using LDL conjugated to colloidal gold, we have examined the ultrastructural effects of chloroquine on lipoprotein uptake by normal cultured fibroblasts.

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