Heat-labile glucose-6-phosphate dehydrogenase in cultured fibroblasts from patients with De Sanctis-Cacchione syndrome

1981 ◽  
Vol 271 (3) ◽  
pp. 341-346
Author(s):  
N. Okada ◽  
Y. Kitano
Keyword(s):  
Cultured Fibroblasts ◽  
Heat Labile ◽  
Glucose 6 Phosphate Dehydrogenase

scholarly journals Studies on Metatherian Sex Chromosomes VII. Glucose-6-phosphate Dehydrogenase Expression in Tissues and Cultured Fibroblasts of Kangaroos

1978 ◽  
Vol 31 (4) ◽  
pp. 415 ◽  
Author(s):  
PG Johnston ◽  
GB Sharman ◽  
Elizabeth A James ◽  
DW Cooper
Keyword(s):  
Sex Chromosomes ◽  
Cultured Fibroblasts ◽  
Glucose 6 Phosphate Dehydrogenase

Expression of the sex-linked enzyme glucose-6-phosphate dehydrogenase (G6PD) was examined electrophoretically in tissues and cultured fibroblasts of female kangaroo heterozygotes ranging in age from 26 days post part urn to adult. All tissues expressed only the maternally derived allele irrespective of which allele was maternal or paternal in origin.

scholarly journals Single Nucleotide Primer Extension (SNuPE) analysis of the G6PD gene in somatic cells and oocytes of a kangaroo (Macropus robustus)

2000 ◽  
Vol 75 (3) ◽  
pp. 269-274 ◽  
Author(s):  
DEBBIE WATSON ◽  
ANITA S. JACOMBS ◽  
DAVID A. LOEBEL ◽  
EDWARD S. ROBINSON ◽  
PETER G. JOHNSTON
Keyword(s):  
Somatic Cells ◽  
Primer Extension ◽  
Paternal Allele ◽  
Transcriptional Level ◽  
Specific Expression ◽  
Single Nucleotide ◽  
Cultured Fibroblasts ◽  
Preferential Expression ◽  
Single Nucleotide Primer Extension ◽  
Glucose 6 Phosphate Dehydrogenase

cDNA sequence analysis of the X-linked glucose-6-phosphate dehydrogenase (G6PD) gene has shown a base difference between two subspecies of the kangaroo, Macropus robustus robustus (wallaroo) and M. r. erubescens (euro). A thymine residue in the wallaroo at position 358 in exon 5 has been replaced by a cytosine residue in the euro, which accounts for the previously reported electrophoretic difference between the two subspecies. This base difference allowed use of the Single Nucleotide Primer Extension (SNuPE) technique to study allele-specific expression of G6PD at the transcriptional level. We began by examining G6PD expression in somatic cells and observed complete paternal X inactivation in all somatic tissues of adult female heterozygotes, whereas we found partial paternal allele activity in cultured fibroblasts, thus confirming previous allozyme electrophoresis studies. In late dictyate oocytes from an adult heterozygote, the assay also detected expression of both the maternal and paternal alleles at the G6PD locus, with the maternal allele showing preferential expression. Thus reactivation of the inactive paternally derived X chromosome occurs during oogenesis in M. robustus, although the exact timing of reactivation remains to be determined.

scholarly journals Altered heat-lability of a fraction of glutathione reductase in aging human lens

1973 ◽  
Vol 134 (4) ◽  
pp. 995-1000 ◽  
Author(s):  
John J. Harding
Keyword(s):  
Protein Synthesis ◽  
Glutathione Reductase ◽  
Phosphoglycerate Kinase ◽  
Intrinsic Property ◽  
Human Lens ◽  
The Core ◽  
Labile Fraction ◽  
Heat Labile ◽  
Glucose 6 Phosphate Dehydrogenase

An unusually heat-labile fraction of glutathione reductase appears in human lens at about an age of 32 years. No such change was found in glucose 6-phosphate dehydrogenase nor in 3-phosphoglycerate kinase. The change is an intrinsic property of glutathione reductase. A greater proportion of the altered glutathione reductase was found in the core, the older part, of the lens. No evidence of a second band of activity was obtained after electrophoresis. Possible interpretations of the results, including errors of protein synthesis, production of a new isoenzyme and post-synthetic changes, are discussed.

scholarly journals Studies on Metatherian Sex Chromosomes VIII. Evidence for an Absence of Dosage Compensation at the Glucose-6-phosphate Dehydrogenase Locus in Cultured Cells of Macropus rufogriseus

1978 ◽  
Vol 31 (4) ◽  
pp. 425 ◽  
Author(s):  
Kathie A Raphael ◽  
DW Cooper
Keyword(s):  
Dosage Compensation ◽  
Sex Chromosomes ◽  
Cultured Cells ◽  
Cultured Fibroblasts ◽  
Glucose 6 Phosphate Dehydrogenase

The level of the sex-linked enzyme glucose-6-phosphate dehydrogenase (G6PD) has been measured in cultured fibroblasts of M. rufogriseus and several other kangaroo species. The data obtained support the conclusion obtained from other evidence that there is a failure of dosage compensation at this locus in fibroblasts of females.

Intralysosomal Accumulation of Colloidal Gold-Low Density Lipoprotein Conjugates in Chloroquine-Treated Fibroblasts

1985 ◽  
Vol 43 ◽  
pp. 546-547 ◽  
Author(s):  
Dean A. Handley ◽  
Cynthia M. Arbeeny ◽  
Larry D. Witte
Keyword(s):  
Colloidal Gold ◽  
Cultured Cells ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Coated Vesicle ◽  
Low Density ◽  
Cholesterol Esters ◽  
Cultured Fibroblasts ◽  
Surface Receptors ◽  
Ldl Particles

Low density lipoproteins (LDL) are the major cholesterol carrying particles in the blood. Using cultured cells, it has been shown that LDL particles interact with specific surface receptors and are internalized via a coated pit-coated vesicle pathway for lysosomal catabolism. This (Pathway has been visualized using LDL labeled to ferritin or colloidal gold. It is now recognized that certain lysomotropic agents, such as chloroquine, inhibit lysosomal enzymes that degrade protein and cholesterol esters. By interrupting cholesterol ester hydrolysis, chloroquine treatment results in lysosomal accumulation of cholesterol esters from internalized LDL. Using LDL conjugated to colloidal gold, we have examined the ultrastructural effects of chloroquine on lipoprotein uptake by normal cultured fibroblasts.

Specificity of receptor-mediated signal transduction by E. coli type its heat-labile toxin

2001 ◽  
Vol 120 (5) ◽  
pp. A700-A700
Author(s):  
S WIMERMACKIN ◽  
R HOLMES ◽  
A WOLF ◽  
W LENCER ◽  
M JOBLING
Keyword(s):  
Signal Transduction ◽  
E Coli ◽  
Heat Labile

EFFECTS OF DEHYDROEPIANDROSTERONE AND ITS CONJUGATES UPON HUMAN GLUCOSE-6-PHOSPHATE DEHYDROGENASE

1969 ◽  
Vol 61 (1_Suppl) ◽  
pp. S46
Author(s):  
Georg W. Oertel ◽  
Peter Menzel ◽  
Ilse Rebelein
Keyword(s):  
Glucose 6 Phosphate Dehydrogenase
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