scholarly journals Developmental Changes in the Late Larva of Calliphora Stygia Iv. Uptake of Plasma Protein by the Fat Body

1971 ◽  
Vol 24 (2) ◽  
pp. 291 ◽  
Author(s):  
Marjoryd Martin ◽  
Judith F Kinnear ◽  
JA Thomson
Keyword(s):  
Protein Content ◽  
Plasma Protein ◽  
Protein Concentration ◽  
Fat Body ◽  
Developmental Changes

The protein content of the larval fat body of O. stygia increases during late third-instar development from 1 �0 mg at day 7 to 3�4 mg in the white prepupa (day 11). This increase is due predominantly to uptake of protein from the haemolymph, in which there is a concurrent fall in protein concentration. The rate of uptake rises from 0�6 mg/day at days 8-9 to 0�9 mg/day at days 10-11.

scholarly journals RECIPROCAL CHANGES IN PLASMA PROTEIN AND PLASMA ACACIA AS RESULT OF HIGH AND LOW PROTEIN DIETS

1950 ◽  
Vol 91 (4) ◽  
pp. 425-431 ◽  
Author(s):  
R. E. Knutti ◽  
J. B. Goetsch ◽  
R. A. Warrick
Keyword(s):  
Protein Content ◽  
Plasma Protein ◽  
Protein Concentration ◽  
High Protein Diet ◽  
The Body ◽  
Protein Diet ◽  
Plasma Protein Concentration ◽  
Gum Acacia ◽  
Normal Limits ◽  
Low Protein

Dogs were made hypoproteinemic by repeated injections of gum acacia, and the acacia injections were discontinued. Diets of varying protein content were then given. When a high protein diet is provided the plasma protein concentration increases; with a low protein diet, or under conditions of fasting, the plasma protein concentration diminishes. Similarly, plasma acacia concentration shows increases and decreases which are reciprocal to the protein variations. Total circulating plasma protein and total circulating plasma acacia show similar changes. In all instances total circulating colloid (acacia plus protein) concentration adds up to an amount within normal limits for protein alone. The results indicate that under these conditions, acacia stored in the body (principally in the liver) can be removed from its site of deposit and returned to the blood. The data also show that dogs in which acacia is deposited in large quantities, require a larger amount of protein in the diet to maintain a constant plasma protein content than do normal dogs. It appears that the mechanism for maintenance of peripheral colloidal material may be dependent on differences in intracellular and extracellular colloidal osmotic pressure. The experiments also support the idea that plasma protein molecules, as well as gum acacia, may pass in and out of cells through the cell membranes.

Extraction and Partial Purification of Protease from Bilimbi (Averrhoa bilimbi L.)

2013 ◽  
Vol 10 (2) ◽  
pp. 29
Author(s):  
Normah Ismail ◽  
Nur' Ain Mohamad Kharoe
Keyword(s):  
Molecular Weight ◽  
Protein Content ◽  
Ammonium Sulfate ◽  
Molecular Weight Distribution ◽  
Weight Distribution ◽  
Protease Activity ◽  
Protein Concentration ◽  
Temperature Stability ◽  
Partial Purification ◽  
Ammonium Sulfate Precipitation

Unripe and ripe bilimbi (Averrhoa bilimbi L.) were ground and the extracted juices were partially purified by ammonium sulfate precipitation at the concentrations of 40 and 60% (w/v). The collected proteases were analysed for pH, temperature stability, storage stability, molecular weight distribution, protein concentration and protein content. Protein content of bilimbi fruit was 0.89 g. Protease activity of both the unripe and ripe fruit were optimum at pH 4 and 40°C when the juice were purified at 40 and 60% ammonium sulfate precipitation. A decreased in protease activity was observed during the seven days of storage at 4°C. Molecular weight distribution indicated that the proteases protein bands fall between IO to 220 kDa. Protein bands were observed at 25, 50 and 160 kDa in both the unripe and ripe bilimbi proteases purified with 40% ammonium sulfate, however, the bands were more intense in those from unripe bilimbi. No protein bands were seen in proteases purified with 60% ammonium sulfate. Protein concentration was higher for proteases extracted with 40% ammonium sulfate at both ripening stages. Thus, purification using 40% ammonium sulfate precipitation could be a successful method to partially purify proteases from bilimbi especially from the unripe stage. 

Changes in rat mesentery interstitial matrix due to superfusate

1993 ◽  
Vol 265 (3) ◽  
pp. H852-H856 ◽  
Author(s):  
B. J. Barber ◽  
R. A. Babbitt ◽  
S. Dutta ◽  
S. Parameswaran
Keyword(s):  
Protein Content ◽  
Normal Saline ◽  
Protein Concentration ◽  
Matrix Protein ◽  
Protein Transport ◽  
Albumin Concentration ◽  
Sprague Dawley ◽  
Tissue Samples ◽  
Rat Mesentery ◽  
Sprague Dawley Rats

Animal preparations for microscopy often require a superfusate solution to cover surgically exposed tissue. There are few, if any, data concerning the effects of this solution on extravascular protein concentration and hydration. The effect of superfusion on mesenteric tissue in anesthetized male Sprague-Dawley rats was studied. Tissue samples were taken from nonsuperfused and superfused tissue and analyzed for hydration, albumin, and transferrin content. The mesenteric tissue interstitial matrix was rapidly altered by normal saline superfusate. After superfusion, there was a decrease (P < 0.01) in tissue albumin concentration from 1.17 +/- 0.27 to 0.10 +/- 0.08 g/dl (n = 9). Tissue hydration increased from 4.98 +/- 0.8 micrograms water/microgram dry wt in controls to 7.38 +/- 1.2 micrograms water/micrograms dry wt after superfusion. When a range of superfusate albumin concentrations was used (0, 1, 2, and 3 g/dl), tissue albumin concentration changed 0.59 +/- 0.09 g/dl for each gram per deciliter change in superfusate concentration (P < 0.0001). The large changes in interstitial matrix protein content and hydration suggest that superfusate solution effects need to be considered in microvascular protein transport experiments.

scholarly journals CHANGES IN THE VOLUME OF PLASMA AND ABSOLUTE AMOUNT OF PLASMA PROTEINS IN NEPHRITIS

1924 ◽  
Vol 39 (6) ◽  
pp. 921-929 ◽  
Author(s):  
G. C. Linder ◽  
C. Lundsgaard ◽  
D. D. Van Slyke ◽  
E. Stillman
Keyword(s):  
Plasma Protein ◽  
Plasma Volume ◽  
Plasma Proteins ◽  
Protein Concentration ◽  
The Body ◽  
Absolute Amount ◽  
Low Protein

1. We have not observed gross increases in plasma volume in glomerulonephritis, nephrosis, or nephrosclerosis, even when the concentration of plasma proteins was much below normal. Our results indicate the probability that "hydremic plethora" does not occur. 2. The low protein concentration frequently observed in the plasma in nephritis is not due to increased plasma volume but to a decrease of the total amount of plasma protein in the body. 3. Changes in plasma volume showed no constant relationship to changes in edema.

Effects of hypoproteinemia-induced myocardial edema on left ventricular function

1998 ◽  
Vol 274 (3) ◽  
pp. H937-H944 ◽  
Author(s):  
M. Miyamoto ◽  
D. E. McClure ◽  
E. R. Schertel ◽  
P. J. Andrews ◽  
G. A. Jones ◽  
...  
Keyword(s):  
Plasma Protein ◽  
Protein Concentration ◽  
Systolic Dysfunction ◽  
Myocardial Edema ◽  
Left Ventricular ◽  
Control Group ◽  
Lv Function ◽  
Stroke Work ◽  
Plasma Protein Concentration ◽  
Total Plasma Protein

In previous studies, we observed left ventricular (LV) systolic and diastolic dysfunction in association with interstitial myocardial edema (IME) induced by either coronary venous hypertension (CVH) or lymphatic obstruction. In the present study, we examined the effects of myocardial edema induced by acute hypoproteinemia (HP) on LV systolic and diastolic function. We also combined the methods of HP and CVH (HP-CVH) to determine their combined effects on LV function and myocardial water content (MWC). We used a cell-saving device to lower plasma protein concentration in HP and HP-CVH groups. CVH was induced by inflating the balloon in the coronary sinus. Six control dogs were treated to sham HP. Conductance and micromanometer catheters were used to assess LV function. Contractility, as measured by preload recruitable stroke work, did not change in control or HP groups but declined significantly (14.5%) in the HP-CVH group. The time constant of isovolumic LV pressure decline (τ) increased significantly from baseline by 3 h in the HP (24.8%) and HP-CVH (27.1%) groups. The end-diastolic pressure-volume relationship (stiffness) also increased significantly from baseline by 3 h in the HP (78.6%) and HP-CVH (42.6%) groups. Total plasma protein concentration decreased from 5.2 ± 0.2 g/dl at baseline to 2.5 ± 0.0 g/dl by 3 h in the HP and HP-CVH groups. MWC of the HP (79.8 ± 0.25%) and HP-CVH groups (79.8 ±0.2%) were significantly greater than that of the control group (77.8 ± 0.3%) but not different from one another. In conclusion, hypoproteinemia-induced myocardial edema was associated with diastolic LV dysfunction but not systolic dysfunction. The edema caused by hypoproteinemia was more than twice that produced by our previous models, yet it was not associated with systolic dysfunction. CVH had a negative inotropic effect and no significant influence on MWC. IME may not have the inverse causal relationship with LV contractility that has been previously postulated but appears to have a direct causal association with diastolic stiffness as has been previously demonstrated.

scholarly journals THE INFLUENCE OF DIFFERENT MOLECULAR WEIGHT SEMINAL PLASMA PROTEIN CONTENT ON SOME FERTILITY PARAMETERS IN BOAR'S EJACULATES

2019 ◽  
Vol 56 (4) ◽  
Author(s):  
Ivan Blagoje Stančić ◽  
Igor Zdraveski ◽  
Saša Dragin ◽  
Jelena Blagoje Apić ◽  
Slobodanka Vakanjac ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Protein Content ◽  
Plasma Protein ◽  
Seminal Plasma ◽  
Different Molecular Weight
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